Jean-Baptiste Manneville

Jean-Baptiste Manneville
French National Centre for Scientific Research | CNRS · Laboratoire Matière et Systèmes Complexes (MSC) CNRS Université Paris Cité (UMR 7057)

PhD

About

63
Publications
12,208
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3,609
Citations
Citations since 2016
31 Research Items
1810 Citations
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300

Publications

Publications (63)
Article
Full-text available
Background In vitro studies have shown that physical parameters, such as membrane curvature, tension, and composition, influence the budding and fission of transport intermediates. Endocytosis in living cells also appears to be regulated by the mechanical load experienced by the plasma membrane. In contrast, how these parameters affect intracellula...
Article
Full-text available
Solid tumors are often first diagnosed by palpation, suggesting that the tumor is more rigid than its surrounding environment. Paradoxically, individual cancer cells appear to be softer than their healthy counterparts. In this review, we first list the physiological reasons indicating that cancer cells may be more deformable than normal cells. Next...
Article
Full-text available
Significance Cell mechanics is crucial for many, if not all, cell functions. Although mechanics at the scale of the whole cell is extensively documented, there are only few methods to measure intracellular mechanics at the local scale. Here, we develop a technique that allows us ( i ) to map the spatial variations of intracellular mechanical parame...
Article
Full-text available
Centrosome positioning is crucial during cell division, cell differentiation, and for a wide range of cell-polarized functions including migration. In multicellular organisms, centrosome movement across the cytoplasm is thought to result from a balance of forces exerted by the microtubule-associated motor dynein. However, the mechanisms regulating...
Article
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Cytoplasmic coat proteins are required for cargo selection and budding of tubulovesicular transport intermediates that shuttle between intracellular compartments. To better understand the physical parameters governing coat assembly and coat-induced membrane deformation, we have reconstituted the Arf1-dependent assembly of the COPI coat on giant uni...
Preprint
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The mechanical properties of cell-cell junctions are critical for the stability of an epithelium. Cell-cell junction ablation experiments are classically used as a readout for junctional mechanics. However, without the knowledge of the viscoelastic properties of the microenvironment of the ablated junction, tensile junctional forces cannot be measu...
Article
Cells tend to soften during cancer progression, suggesting that mechanical phenotyping could be used as a diagnostic or prognostic method. Here we investigate the cell mechanics of gliomas, brain tumors that originate from glial cells or glial progenitors. Using two microrheology techniques, a single cell parallel plates rheometer to probe whole ce...
Preprint
Full-text available
Cancer cell softening increases with the progression of the disease, suggesting that mechanical phenotyping could be used as a diagnostic or prognostic method. Here we investigate the cell mechanics of gliomas, brain tumors that originate from glial cells or glial progenitors. We use two microrheology techniques, a single cell parallel plates rheom...
Article
Salmonella enterica is an intracellular bacterial pathogen. The formation of its replication niche, which is composed of a vacuole associated with a network of membrane tubules, depends on the secretion of a set of bacterial effector proteins whose activities deeply modify the functions of the eukaryotic host cell. By recruiting and regulating the...
Article
Membrane trafficking plays a crucial role in cell polarity by directing lipids and proteins to specific subcellular locations in the cell and sustaining a polarized state. The Golgi apparatus, the master organizer of membrane trafficking, can be subdivided into three layers that play different mechanical roles: a cytoskeletal layer, the so-called G...
Article
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Significance This study shows that an oncogenic mammary epithelial cell surrounded by normal cells can extrude basally in vivo and invade surrounding tissues without formation of a primary tumor. Here, we show that overexpression of the key polarity protein atypical protein kinase C ι (aPKCi) is sufficient for triggering basally oriented epithelial...
Article
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Molecular motors play important roles in force generation, migration and intracellular trafficking. Changes in specific motor activities are altered in numerous diseases. KIF20A, a motor protein of the kinesin-6 family, is overexpressed in bladder cancer, and KIF20A levels correlate negatively with clinical outcome. We report here a new role for th...
Preprint
Full-text available
Molecular motors play important roles in force generation, migration and intracellular trafficking. Changes in specific motor activities are altered in numerous diseases. KIF20A, a motor protein of the kinesin-6 family, is overexpressed in bladder cancer, and KIF20A levels correlate negatively with the clinical outcome. We report here a new role fo...
Article
Full-text available
Extracellular matrix (ECM) mechanical cues have powerful effects on cell proliferation, differentiation and death. Here, starting from an unbiased metabolomics approach, we identify synthesis of neutral lipids as a general response to mechanical signals delivered by cell–matrix adhesions. Extracellular physical cues reverberate on the mechanical pr...
Article
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Uveal melanoma (UM) is an aggressive tumor in which approximately 50% of patients develop metastasis. Expression of the PTP4A3 gene, encoding a phosphatase, is predictive of poor patient survival. PTP4A3 expression in UM cells increases their migration in vitro and invasiveness in vivo. Here, we show that CRMP2 is mostly dephosphorylated on T514 in...
Article
The amphipathic lipid packing sensor (ALPS) motif of ArfGAP1 brings this GTPase activating protein to membranes of high curvature. Phospholipases are phospholipid-hydrolyzing enzymes that generate different lipid products that alter the lateral organization of membranes. Here, we evaluate by fluorescence microscopy how in-situ changes of membrane l...
Preprint
Full-text available
Lipid packing defects favor the binding of proteins to cellular membranes by creating spaces between lipid head groups that allow the insertion of amphipathic helices or lipid modifications. The density of packing defects in a lipid membrane is well known to increase with membrane curvature and in the presence of conical-shaped lipids. In contrast,...
Data
Appendix S1. Supplemental text (material and methods not necessary to understand the overall message of the paper, but required to reproduce the experiments) and supplemental references. Figure S1. Control experiment to demonstrate that RAB and GST proteins are only recruited to membranes if they are prenylated. Figure S2. Graphical illustration...
Article
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Specific intracellular localization of RAB GTPases has been reported to be dependent on protein factors but the contribution of the membrane physicochemical properties to this process has been poorly described. Here, we show that three RAB proteins (RAB1/RAB5/RAB6) preferentially bind in vitro to disordered and curved membranes, and that this featu...
Data
Movie S1. EndoA2 N-BAR Domain Does Not Induce Scission of Tubular Membranes, Related to Figure 1 and Figure S1 Confocal fluorescence time-lapse during injection of an N-BAR domain of endoA2 near a tube pulled from a micropipette-aspired GUV. Movie shows spontaneous tubulation of the GUV and the formation of a scaffold on the tube (causing it to bu...
Data
Confocal fluorescence time lapse showing a scission event of a kinesin-pulled tube connected to a GUV seconds after endoA2 injection into the system. Shown is a different example from Movie S4. Scale bar, 2 μm.
Data
Movie shows extension of a tube partially scaffolded by endoA2 N-BAR domain leading up to scission at the tube-GUV interface. Shown are the confocal fluorescence time lapse (left) and the tube retraction force, f, both changing with time, t. Top left: N-BAR (green); center left: lipids (red); bottom left: overlay
Data
Movie shows extension of a tube initially fully scaffolded by endoA2 N-BAR domain leading up to scission. After initial extension, gaps form in the scaffold making fully and partially scaffolded tubes equivalent in FDS. Shown are confocal fluorescence time lapse (left) and tube retraction force, f, both changing with time, t. Top left: N-BAR (green...
Data
Confocal fluorescence time lapse showing two scission events of kinesin-pulled tubes connected to a GUV seconds after endoA2 injection into the system. Scale bar, 2 μm.
Article
Full-text available
Membrane scission is essential for intracellular trafficking. While BAR domain proteins such as endophilin have been reported in dynamin-independent scission of tubular membrane necks, the cutting mechanism has yet to be deciphered. Here, we combine a theoretical model, in vitro, and in vivo experiments revealing how protein scaffolds may cut tubul...
Article
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Tight regulation of integrin activity is paramount for dynamic cellular functions such as cell matrix adhesion and mechanotransduction. Integrin activation is achieved through intracellular interactions at the integrin cytoplasmic tails and through integrin–ligand binding. In this study, we identify the metabolic sensor AMP-activated protein kinase...
Data
Full-text available
The mechanical properties of cells impact on their architecture, their migration, intracellular trafficking, and many other cellular functions and have been shown to be modified during cancer progression. We have developed an approach to map the intracellular mechanical properties of living cells by combining micropatterning and optical tweezers-ba...
Article
Full-text available
Weibel-Palade body (WPB)-actin interactions are essential for the trafficking and secretion of von Willebrand factor, yet, the molecular basis for this interaction remains poorly defined. Myosin Va (MyoVa) is recruited to WPBs by a Rab27A-MyRIP complex and is thought to be the prime mediator of actin binding, however, direct MyRIP-actin interaction...
Article
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Phosphoinositides play a central role in many physiological processes by assisting the recruitment of proteins to membranes through specific phosphoinositide-binding motifs. How this recruitment is coordinated in space and time is not well understood. Here we show that BIN1/M-Amphiphysin2, a protein involved in T-tubule biogenesis in muscle cells a...
Article
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Sorting of membrane proteins within the secretory pathway of eukaryotic cells is a complex process involving discrete sorting signals as well as physico-chemical properties of the transmembrane domain (TMD). Previous work demonstrated that tail-anchored (TA) protein sorting at the interface between the Endoplasmic Reticulum (ER) and the Golgi compl...
Article
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Microrheology is the investigation of the mechanical deformation of a given material in response to an applied stress at the micrometer scale. Microrheology allows to probe the microenvironment inside the cell in passive or active manner. Here we measure the cytoplasm viscoelastic properties using intracellulaer active microrheology technique. We u...
Article
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Arfaptin2 contains a Bin/Amphiphysin/Rvs (BAR) domain and directly interacts with proteins of the Arf/Arl family in their active GTP-bound state. It has been proposed that BAR domains are able to sense membrane curvature and to induce membrane tubulation. We report here that active Arf1 is required for the recruitment of Arfaptin2 to artificial lip...
Article
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The last step of cell division, cytokinesis, produces two daughter cells that remain connected by an intercellular bridge. This state often represents the longest stage of the division process. Severing the bridge (abscission) requires a well-described series of molecular events, but the trigger for abscission remains unknown. We found that pulling...
Article
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In recent years, the interest for proteins that exert key functions in vesicular trafficking through their ability to sense or induce positive membrane curvature has expanded. In this chapter, we first present simple protocols to determine whether a protein targets positively curved membranes with liposomes of well-defined size. Next we describe mo...
Article
Membrane transport between intracellular compartments, entry or exit out of the cell, imply similar sequential events: membrane deformation and lipid/protein sorting during the formation of the transport intermediate (vesicle or tube), fission from the donor compartment, transport and eventually fusion with the acceptor membrane. The mechanisms beh...
Article
Indole is a very important biological molecule being produced by more than 85 Gram positive and Gram negative bacterial species, including Escherichia coli. Among its several physiological functions indole is shown to stop bacterial cell division when secreted in large quantities. Here we study the effect of indole on the electrical properties of t...
Article
Full-text available
Coat proteins orchestrate membrane budding and molecular sorting during the formation of transport intermediates. Coat protein complex I (COPI) vesicles shuttle between the Golgi apparatus and the endoplasmic reticulum and between Golgi stacks. The formation of a COPI vesicle proceeds in four steps: coat self-assembly, membrane deformation into a b...
Article
Full-text available
The generation of membrane curvature in intracellular traffic in-volves many proteins that can curve lipid bilayers. Among these, dynamin-like proteins were shown to deform membranes into tubules, and thus far are the only proteins known to mechanically drive membrane fission. Because dynamin forms a helical coat circling a membrane tubule, its pol...
Article
Full-text available
The generation of membrane curvature in intracellular traffic involves many proteins that can curve lipid bilayers. Among these, dynamin-like proteins were shown to deform membranes into tubules, and thus far are the only proteins known to mechanically drive membrane fission. Because dynamin forms a helical coat circling a membrane tubule, its poly...
Article
Full-text available
ArfGAP1, which promotes GTP hydrolysis on the small G protein Arf1 on Golgi membranes, interacts preferentially with positively curved membranes through its amphipathic lipid packing sensor (ALPS) motifs. This should influence the distribution of Arf1-GTP when flat and curved regions coexist on a continuous membrane, notably during COPI vesicle bud...
Article
Full-text available
Sorting of lipids and proteins is a key process allowing eukaryotic cells to execute efficient and accurate intracellular transport and to maintain membrane homeostasis. It occurs during the formation of highly curved transport intermediates that shuttle between cell compartments. Protein sorting is reasonably well described, but lipid sorting is m...
Article
Full-text available
Sorting of lipids and proteins is a key process allowing eukaryotic cells to execute efficient and accurate intracellular transport and to maintain membrane homeostasis. It occurs during the formation of highly curved transport intermediates that shuttle between cell compartments. Protein sorting is reasonably well described, but lipid sorting is m...
Article
Several studies have shown that lipids are sorted at every step of intracellular trafficking [1], for example in [2] it has been shown that COPI-coated vesicles have a different lipid composition than the Golgi apparatus they originate from. But general principles governing lipid sorting are not fully understood yet. In particular, general physical...
Article
Full-text available
Centrosome positioning is tightly controlled throughout the cell cycle and probably shares common regulatory mechanisms with spindle-pole positioning. In this article, we detail the possible mechanisms controlling centrosome and spindle positioning in various organisms both in interphase and mitotic cells, and discuss recent findings showing how mi...
Article
Full-text available
Total internal reflection fluorescence (TIRF) is the technique of choice to visualize and quantify cellular events localized at the basal plasma membrane of adherent cells. By selectively illuminating the first 200 nm above the basal membrane, it allows maximal resolution in the vertical z-axis. In this chapter, I describe a prism-based TIRF setup...
Article
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Cell polarization is essential in a wide range of biological processes such as morphogenesis, asymmetric division, and directed migration. In this study, we show that two tumor suppressor proteins, adenomatous polyposis coli (APC) and Dlg1-SAP97, are required for the polarization of migrating astrocytes. Activation of the Par6-PKCzeta complex by Cd...
Article
Full-text available
The role of cytoskeletal elements in regulating transport and docking steps that precede exocytosis of secretory organelles is not well understood. We have used Total Internal Reflection Fluorescence (TIRF) microscopy to visualize the three-dimensional motions of secretory organelles near the plasma membrane in living endothelial cells. Weibel-Pala...
Article
Full-text available
Autosomal dominant GH deficiency type II (IGHDII) is often associated with mutations in the human GH gene (GH1) that give rise to products lacking exon-3 ((Deltaexon3)hGH). In the heterozygous state, these act as dominant negative mutations that prevent the release of human pituitary GH (hGH). To determine the mechanisms of these dominant negative...
Article
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There is considerable interest in the mechanisms that drive and control the spread of morphogens in developing animals. Although much attention is given to events occurring after release from expressing cells, release itself could be an important modulator of range. Indeed, a dedicated protein, Dispatched, is needed to release Hedgehog from the sur...
Article
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We present a detailed analysis of the micropipet experiments recently reported by J-B. Manneville et al., [Phys. Rev. Lett. 82, 4356 (1999)], including a derivation of the expected behavior of the membrane tension as a function of the areal strain in the case of an active membrane, i.e., containing a nonequilibrium noise source. We give a general e...
Article
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Endothelium of the cerebral blood vessels, which constitutes the blood-brain barrier, controls adhesion and trafficking of leukocytes into the brain. Investigating signaling pathways triggered by the engagement of adhesion molecules expressed on brain endothelial cells using two rat brain endothelial cell lines (RBE4 and GP8), we report in this pap...
Article
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We report the first experimental evidence of the effect of the activity of transmembrane proteins on shape fluctuations of a lipid membrane. We incorporate a light-driven proton pump, the bacteriorhodopsin (BR), inside the phospholipid bilayer of fluctuating giant vesicles. Using the micropipet technique, we measure the excess surface area due to t...
Article
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Membranes in thermal equilibrium are well known to exhibit Brownian motion type shape fluctuations. Membranes containing active force centers -- such as chemically active membrane proteins -- suffer additional non-equilibrium shape fluctuations due to the activity of these force centers. We demonstrate, using scaling arguments, that non-equilibrium...
Article
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Laboratory experiments of thermal convection in rotating spherical shells exhibit symmetric bands and alternating zonal jets in a thin layer near the outer boundary at high rotation rates and at Rayleigh numbers far in excess of the critical value. The bands result from large-scale ordering of small-scale columnar convection deeper in the shell and...

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Projects (5)
Project
Cdc42 is an evolutionaryly conserved RhoGTPase protein in organisms ranging from yeasts to mammals. It's role in the plasma membrane is well studied and explores. Whereas there exists a distinct organellar pool of Cdc42 in the Golgi apparatus whose role is of interest to several scientist in the field now. My current project aims at better understanding the regulation and functions of this distinct pool.