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26
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Introduction
Skills and Expertise
Additional affiliations
June 2014 - June 2015
June 2013 - June 2014
February 2010 - August 2012
Publications
Publications (26)
Introduction: Oral carcinogenesis is a multistage process, featuring genetic and molecular alterations leading to rapid cell division, invasion, metastasis, and increased cell survival. Many of these alterations are due to perturbations in the cell signaling networks, which in turn lead to constitutive deregulation of the proteins involved in the r...
Simple Summary
Exosomes are vesicles that vary between 40 and 150 nm in diameter and are secreted by cells. These carry miRNAs that have various roles in intercellular signaling. They have different biological functions and are unique to the secreting cell. This provides information about the state of the cell and its involvement in pathological pr...
14-3-3s are a family of structurally similar proteins that bind to phosphoserine or phosphothreonine residues, forming the central signaling hub that coordinates or integrates various cellular functions, thereby controlling many pathways important in cancer, cell motility, cell death, cytoskeletal remodeling, neuro-degenerative disorders and many m...
Diabetes mellitus is one of the major causes of death worldwide, due to the complexity of the diseases associated with it, for which treatment is far from complete. Since synthetic hypoglycemic are often associated with side effects, the study was designed to evaluate the hypoglycemic activity of a herbal drug Semecarpus anacardium, as herbal drugs...
Successful metastasis depends on cell invasion, migration, host immune escape, extravasation, and angiogenesis. The process of cell invasion and migration relies on the dynamic changes taking place in the cytoskeletal components; actin, tubulin and intermediate filaments. This is possible due to the plasticity of the cytoskeleton and coordinated ac...
Abstract Connexin36 (Cx36) is the most abundant connexin in central nervous system neurons. It forms gap junction channels that act as electrical synapses. Similar to chemical synapses, Cx36-containing gap junctions undergo activity-dependent plasticity and complex regulation. Cx36 gap junctions represent multimolecular complexes and contain cytosk...
Calmodulin binding is a nearly universal property of gap junction proteins, imparting a calcium-dependent uncoupling behavior that can serve in an emergency to decouple a stressed cell from its neighbors. However, gap junctions that function as electrical synapses within networks of neurons routinely encounter large fluctuations in local cytoplasmi...
Recent reports highlight the potential tumorigenic role of Dentin Sialophosphoprotein (DSPP) and its cognate partner Matrix Metalloproteinase 20 (MMP-20) in Oral Squamous Cell Carcinomas (OSCCs). However, the function/mechanism of these roles is yet to be fully established. The present study aimed to investigate the effects of DSPP and MMP20 silenc...
Background
Matrix metalloproteinases‐20 (MMP20) expression is widely regarded as tooth specific, with expression limited to dental hard tissues. Recently, we reported MMP20 expression and interaction with dentin sialophosphoprotein (DSPP), a member of the Small Integrin Binding Ligand N‐linked Glycoproteins (SIBLINGs), in human oral squamous cell c...
Dentin sialophosphoprotein (DSPP) is upregulated in various human cancers, including head and neck squamous cell carcinoma. Cancer cells are commonly found under constant endoplasmic reticulum (ER) stress and exhibit increased levels of misfolded proteins, due to gene mutations and a stressful microenvironment. The present study examined the effect...
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases encoded by 24 distinct genes. Their functions have been implicated in numerous normal and pathologic processes, including uterine involution and organogenesis, inflammation and wound healing, vascular and autoimmune disease progression. Pertinent to this review, the role of MMPs in...
Equilibrative nucleoside transporters (ENTs) are integral membrane proteins, which reside in plasma membranes of all eukaryotic cells and mediate thermodynamically downhill transport of nucleosides. This process is essential for nucleoside recycling, and also plays a key role in terminating adenosine-mediated cellular signaling. Furthermore, ENTs m...
Glucose produced by gluconeogenesis and glycogenolysis plays an important role in aggravating hyperglycemia in diabetes, and altered mitochondrial function is associated with impaired energy production. The present study focuses on the effect of Semecarpus anacardium on carbohydrate metabolism and energy production in diabetic rats. Diabetes was in...
Leakage of cellular enzymes into the plasma is a clear indication of cell damage. When liver plasma membrane is damaged, a variety of enzymes normally located in the cytosol are released into the blood stream and their estimation is a quantitative marker for the extent of damage. The cytoprotective effect of Semecarpus anacardium was evaluated in r...
Alterations in lipid metabolism and lipoprotein disturbances have played an important role in increasing the risk of cardiovascular mortality and morbidity in diabetes. A drug that has hypoglycemic activity can be used for the treatment of hyperlipidemia also. The present study was carried out to evaluate the hypolipidemic activity of Semecarpus an...
Oxidative and nitrosative stress play an important role in the complications of diabetes mellitus. Free radicals are produced when there is an electron leak in the mito-chondria and a change in the mitochondrial membrane potential. The present study was undertaken to investigate the role of Semecarpus anacardium in protecting the mito-chondria by m...
Diabetes mellitus is one of the major causes of death worldwide, due to the complexity of the diseases associated with it, for which treatment is far from complete. Since synthetic hypoglycemic are often associated with side effects, the study was designed to evaluate the hypoglycemic activity of a herbal drug Semecarpus anacardium , as herbal drug...
Questions
Questions (60)
Hi All,
I would like to know if there is a way to superimpose serial sections of tissues after doing immunofluorescence. The problem is I want to see the co-localization between few proteins and the antibodies are either mouse or rabbit. (No other species available). So I can only add two antibodies at a time. I was thinking if it would be possible to do the IF on serial tissue sections and superimpose on each other like the way people do for cryoEM. Any input or help would be appreciated.
Thanks
hi,
Is there anyone out there who has used R18 inhibitor peptide for inhibiting 14-3-3 in vitro. If yes could you let me know the concentration and does it need any peptide transfection reagent for it to penetrate the cells. Thanks in advance.
Regards,
jaya
Hi,
Im trying to use the image segmentation tool in image J for my image and Iam not getting it correctly to find out the co-localization of two proteins. If anyone has used it, could you please help me.
Regards,
jaya
Hi,
Iam doing IF in HeLa cells and facing a weird problem. I use the standard protocol and when I expose the cell to laser light in the 488 channel, all the fluorescence fades the instant its exposed to light. Never faced this problem before. Used the same mounting medium and secondary antibody without any problem previously. Decreased the epifluorescence light even to 10%, but still the same thing happened thrice. No recovery of fluorescence later on. Gone once for all.
Has anyone faced this problem before. leads would be appreciated.
Thanks,
jaya
Hi,
Is anyone doing IP/Co-IP for actin. If yes could you please share the protocol.
Thanks
jaya
Hi,
Iam trying to do a co-IP for actin after Ip with PP2A antibody, but Iam not able to detect actin in the IP fraction. The detergent that I use is maltopyranoside. If I do the IP without the detergent, then iam able to see the actin bands. Is there any other detergent that can be used to IP/Co-IP actin. Iam going to use this for actin binding proteins. Any leads would be appreciated.
jaya
