Javier Roo

University of Las Palmas de Gran Canaria | ULPGC · Research Group of Aquaculture

In a time of unprecedented coral reef decline, improved aquaculture protocols and high-quality live feeds may contribute to the sustainability of the ornamentals industry producing new valuable and stronger specimens bred under controlled conditions, mitigating the pressure on wild natural stocks. In the present study, the effect of microalgae enri...

Most emerging species in marine aquaculture and the aquarium industry have a sensitive and small-mouthed larval stage, and their culture is very challenging due to a lack of appropriate early feeding protocols. The use of copepods has been widely recognized as a good alternative to traditional fish live feeds (rotifers and Artemia spp.), especially...

Most commercial products available for Artemia sp. enrichment include high levels of docosahexaenoic acid (DHA) to boost its nutritional value, though with limited success. In this regard, the present study evaluated the alternative utilization of eicosapen-taenoic acid (20 : 5n-3; EPA) oils to improve the n-3 long-chain highly unsaturated fatty ac...

Simple Summary Sustainable aquaculture expansion will be crucial for the food security of a growing population expected to reach ~10 billion by 2050. Larval rearing is one of the most complex phases of marine aquaculture due to the need for additional facilities and labor to produce zooplankton to feed the larvae. Therefore, establishing adequate p...

A 12-day experiment was conducted to investigate the effects of Artemia enrichment with two experimental microalgal emulsions (formulated with selected fatty acid contents) on Penaeus vannamei postlarvae. For this purpose, 405,000 postlarvae (stage 1) were obtained from a commercial hatchery in Santa Elena, Ecuador, and distributed into nine fiberg...

The fish body shape is a key factor that influences multiple traits such as swimming, foraging, mating, migrations, and predator avoidance. The present study describes the body morphological changes and the growth trajectories during the transformation from 24 to 54 days post-hatching in the golden grey mullet, Chelon auratus, using geometric morph...

Mullets (Mugilidae) present significant potential for sustainable aquaculture diversification due to their eurythermal, euryhaline, and low-trophic nature. However, the physiological differences and optimal cultured conditions among the diverse mullet species are quite unknown. For these reasons, the present study aimed to address two main objectiv...

The golden grey mullet (Liza aurata) is a promising species for aquaculture's sustainable expansion. However, the lack of sustainable juvenile provision, mainly related to the lack of reproductive control, is one of the most significant bottlenecks for further expanding the culture of this species. In many cases, mullet broodstock management needs...

The reverse osmosis seawater desalination plants open-intake requires different strategies of pre-treatment to reduce the biofouling and scaling. The UltraFiltration (UF) technology is a recent application to this process. In this work, some alternatives to chemical products reducing in chemically enhanced backwash (CEB) and clean in place (CIP) of...

For this experimental study, we set two sampling times for fish ages 24 and 54 days post hatching (dph). To characterize the morphology of body shape was used GMA methodology followed the landmarks-based method. The ETS activity was measured following the method modified by Gómez et al. (1996). To determine biomass in terms of protein was used the...

Vitamin D is an essential fat soluble micronutrient that helps in growth, bone development, calcium homeostasis and other metabolic process. The study on effect of vitamin D3 in marine fish larvae were very scarce irrespective of species. The present study determines the impacts of dietary vitamin D3 on growth performance, calcium absorption, miner...

Osmotic costs in teleosts are highly variable, reaching up to 50% of energy expenditure in some. In several species, environmental salinities close to the isosmotic point (~15 psu) minimize energy demand for osmoregulation while enhancing growth. The present study aimed to characterize the physiological status related to osmoregulation in early juv...

Optimizing the growth and survival of marine fish larvae, while reducing the cost of production, is important for the development of viable hatcheries on an industrial scale. This study was aimed to determine whether Artemia sp. use could be minimized while maintaining high growth and survival, and low skeleton anomalies occurrence in greater amber...

Among of the actual challenges for the sustainable aquaculture development are to lower the trophic level of the cultured species, and to increase the use of by-products from the primary sector. The golden mullet (Liza aurata) is a marine low trophic level consumer present in the Mediterranean and in the Canary Islands, where there was a significan...

This paper is based on the study for the evaluation of the processes of reuse and recycling of reverse osmosis components and membranes in the Canary Islands and Macaronesia, within the DESAL+ project and in the framework of the DESAL+ LIVING LAB platform, coordinated by the Canary Islands Technological Institute (ITC) and the Canary Islands Agency...

The main objective of this study was to determine the effect of two feeding additives (cMOS: Actigen®, Alltech Inc., USA) and a phytogenic specifically designed for reducing ectoparasite incidence, combining garlic and labiatae herbal extracts (HERB; Delacon, Austria) on growth performance, stress and immune response of greater amberjack (Seriola d...

Abstract In previous studies, combined inclusion of Zn, Mn and Se in early weaning diets improved larval growth, but suggested a potential toxicity by one of these elements. The present study aimed to determine the effect of the single inclusion of Zn, Mn, Se or Cu, their combination (Control+) or their absence (Control−) on larval diets. At the en...

Dietary lipids and fatty acids composition play an important role in the reproductive processes, embryo development and larval survival in marine fish. Long-chain polyunsaturated fatty acids (LC-PUFA) are especially important for egg and larval quality. The main objective of the present study was to evaluate the effects of increasing dietary n-3 LC...

The Aquaculture Group (GIA/IU-ECOAQUA) of the University of Las Palmas de Gran Canaria (ULPGC), in Gran Canaria Island, Spain has been working on greater amberjack (Seriola dumerili) culture since 2006 with important contributions in different R&D programs at regional, national and international levels. GIA is currently coordinating the SERIOLA pro...

Four different wreckfish (Polyprion americanus) broodstock batches were maintained in research facilities under different photo and thermo-period conditions, one in Greece, the Helenic Center for Marine Research (HCMR, n = 3) and three in Spain: Instituto Español de Oceanografía (IEO, n = 13) in Vigo, Aquarium Finisterrae (MC2, n = 21) in A Coruña...

To characterise the progression of lipid digestion capacity in gilthead seabream across life cycle, the activities of bile salt-activated lipase (BAL) and phospholipase A2 (PLA2) were determined in the digestive tracts of cultured gilthead seabream from first feeding to marketable size (49 μg to 300 g). Four trials were undertaken with gilthead sea...

Abstract To characterise the progression of lipid digestion capacity in gilthead seabream across life cycle, the activities of bile salt-activated lipase (BAL) and phospholipase A2 (PLA2) were determined in the digestive tracts of cultured gilthead seabream from first feeding to marketable size (49 μg to 300 g). Four trials were undertaken with gil...

Elevated mortality during early stages and high occurrence of skeletal anomalies are major concerns in Greater amberjack (Seriola dumerili) juvenile's production. Identification of nutritional requirements during live prey feeding stages, particularly those related with n-3 HUFA would help to formulate specific diets for this species improving larv...

A well-balanced diet adapted to fulfil the specific nutritional requirements of greater amberjack (Seriola dumerili) would contribute to optimize reproduction and spawning quality. The main objective of the present study was to examine the effects of supplemental histidine, taurine and protein in broodstock diets, on egg quality of greater amberjac...

Production of sufficient high‐quality eggs of greater amberjack (Seriola dumerili) still constitutes the main bottleneck for commercial production of this species. The main objective of this study was to compare the quality of spontaneous spawn of greater amberjack with those obtained by either GnRHa injection or GnRHa implant protocols. Captive am...

The aim of present work is to report the advances in the last three years of studies, such as the obtention of viable spawns and first larval rearing trials applying both intensive and semi-intensive techniques for larval rearing of this specie.

Five isoproteic (54.8%) and isolipidic (24.1%) microdiets, which varied in their docosahexaenoic acid (DHA) content (0.25%, 0.75%, 1.64%, 1.99% and 3.17%; dw), were manufactured to determine its effects on longfin yellowtail Seriola rivoliana larvae in terms of fish biological performance, whole body fatty acid profile and incidence of skeletal ano...

This presentation will review the most recent advances obtained in broodstock and larval rearing management of greater amberjack (Seriola dumerilli) during the last three years at the Aquaculture Research Group (GIA) from the University of Las Palmas de Gran Canaria. A series of studies on hormonal induction strategies, broodstock nutrition, larval...

Skull, pectoral and pelvic fin bone structures in longfin yellowtail Seriola rivoliana were studied from 3.43 ± 0.15 to 16.20 ± 0.73 mm standard length (SL) specimens. The S. rivoliana skull started to mineralize with the appearance of the parasphenoid and maxillary by 3.43 ± 0.15 mm SL at the neurocranium and jaw regions, respectively. The first p...

AQUACULTURE EUROPE 2016

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Levels of the oxidative stress-related minerals selenium (Se), zinc (Zn) and manganese (Mn) that should be supplied in microdiets for marine fish larvae depend on the availability of the molecular form of these minerals. The objective of this study was to determine how effectively Se, Zn and Mn in organic, inorganic and nanoparticle forms promote l...

This study aimed to determine the effects of increasing docosahexaenoic acid (DHA) levels in Sparus aurata diets supplemented with antioxidants vitamins (vitamins C and E). Six isoproteic and isolipidic formulated diets, which varied in the DHA content (38–78 g kg−1 TFA), were fed to 19 dph S. aurata larvae. No significant differences were found in...

Linked in: https://plus.google.com/u/0/photos/107903469917070748871/albums/6090955946921181505/6090955944405626290

Review of Striped Jack culture in Canary Islands from broodstock management to pilot scale juvenile production.

The present study aimed to evaluate the effect of the supplementation of different crab zoeas to enriched Artemia basal diet for Octopus vulgaris paralarvae during the first month of life. Paralar- vae were fed using enriched Artemia nauplii alone and Artemia co-fed either first zoea stages of Grapsus adscensionis or Plagusia depressa. The experime...

Eighteen mature meagre (Argyrosomus regius) broodstock, born in captivity, were divided in 3 experimental groups in ratios of 3 ♀ and 3 ♂ each. One group was inducted by injections of hCG, the other with GnRHa and the last group with implants of GnRHa. In all three treatments all females responded positively to the Hormonal treatment. Statistically...

In the present study, the effect of three different hormonal treatments were tested (GnRHA and hCG injected and GnRHa implanted), in meagre (Argyrosomus regius) broodstock born in captivity, and their production. The highest productions per kg of female and spawn: number of eggs, fertilized eggs, hatched larvae, and larvae 3 days old after hatching...

In this experiment, quality spawns were compared, in mature meagre broodstock (Argyrosomus regius), born in captivity, treated with three hormonal treatments: injection GnRHa and hCG, and implants of GnRHa. The best results in all quality parameters, were obtained in the spawns of the broodstock injected with GnRHa, having 98,7 + 1,4% of fertilized...

For this study, a total of 19 greater amberjack (Seriola dumerili) broodstock were used. Females with oocites bigger than 600µ, and fluent males with a mean weight of 10.72±1.22 kg, fameles, and 10.77±2.33 kg males. The experimental tanks were circular tanks of 40 m3 capacity. One of the tanks were not induced and the other two were with GnRHa, one...

Greater amberjack (Seriola dumerili) broodstock, with a median weight of 10.94±1.21 kg, for females and 11.69±2.35 kg, males, were distributed in two circular tanks of 40 m3. Tank 1, three males and three females were intramuscular injected with GnRHa at a dose of 20 µg.kg-1body weights. The three males and three females of tank 2 were induced with...

For this study, a total of 19 grater amberjack (Seriola dumerili) broodstock were used. Females with, oocytes bigger than 600µ, and fluent males with a mean weight of 10.72±1.22 kg, females, and 10.77±2.33 kg males. The experimental tanks were circular tanks of 40 m3 capacity. One of the tanks was not induced and the other two were with GnRHa, one...

The present study provides a description of the morphological, histological and histochemical development, and the ecological implications of red porgy larvae reared in mesocosm, from hatching to 30 days after hatching (DAH). Four development stages were defined by body total length (TL), in agreement with major patterns of larval organogenesis. Th...

The present study aimed to determine the spawning efficacy, egg quality and quantity of captive breed meagre induced with a single gonadotrophin-releasing hormone agonist (GnRHa) injection of 0, 1, 5, 10, 15, 20, 25, 30, 40 or 50 μg kg–1 to determine a recommended optimum dose to induce spawning. The doses 10, 15 and 20 μg kg–1 gave eggs with the h...

Bone and fins development in Seriola rivoliana were studied from cleared and stained specimens from 3 to 33 days after hatching. The vertebral column began to mineralize in the neural arches at 4.40 ± 0.14 mm Standard Length (SL), continued with the haemal arches and centrums following a cranial-caudal direction. Mineralization of the caudal fin st...

To optimize broodstock management for Seriola rivoliana, a survey over 5 years was performed to evaluate the effects of successive hormonal inductions with GnRHa or dietary regime on spawning quality. Running males and females with an oocyte diameter > 500 μm were injected with GnRHa. The spawning quality was compared among consecutive years and di...

Aquaculture development in marine fish is mainly based on a few species, therefore diversification is a priority. The aim of the present study was to obtain spawns of great amberjack (Seriola dumerili) by induction with gonadotropin releasing hormone analogue (GnRHa) injection or implant and determine their effects on eggs quality and economic perf...

Aquaculture development in marine fish is mainly based on a few species, therefore diversification is a priority. The aim of the present study was to obtain spawns of great amberjack (Seriola dumerili) by induction with gonadotropin releasing hormone analogue (GnRHa) injection or implant and determine their effects on eggs quality and economic perf...

The objective of the present study was to compare the effectiveness of dietary marine phospholipids (MPL) obtained from krill and soybean lecithin (SBL) on the rearing performance and development of seabream (Sparus aurata) larvae. Larvae were fed from 16 to 44 day posthatching (dph) five formulated microdiets with three different levels (50, 70 an...

Although dietary marine phospholipids are able to improve culture performance of marine fish larvae in a further extend than soybean lecithin, both types of phospholipids (PL) markedly increase oxidative risk. The inclusion of a fat-soluble antioxidant such as the vitamin E α-tocopherol could allow a better control of oxidative stress. The objectiv...

The inclusion of complementary antioxidative factors, such as selenium (Se), could counteract the high oxidation risk in early weaning diets high in polyunsaturated fatty acids (PUFA). The present study investigated the effects of graded levels of Se derived yeast with krill phospholipids (KPL) on skeletal development, survival, stress resistance,...

Linked in: https://plus.google.com/u/0/photos/107903469917070748871/albums/6090955946921181505/6090955944405626290. This video summarizes results of the project : improvements of larval rearing techniques for seriola culture (METCSER) 2011-2014. Cofunded by the Canary Goberments and EU(FEDER).

Meagre (. Argyrosomus regius) have good potential for aquaculture. Culture characteristics include controlled spawning in captivity, relatively easy larval rearing, fast growth, good feed conversion ratios and no maturation during ongrowing. Whilst characteristics of the final product include good processing yield, low fat content, good taste and f...

The lack of specific compound diets for cephalopods is limiting the industrial development of some species. In this study, four agglutinated moist diets were tested in individually reared Octopus vulgaris (979 ± 151 g) for 8 weeks. All diets were based on bogue Boops boops, accidentally reared in fish farms (aquaculture by-product), and agglutinate...

Most nutritional studies on Octopus vulgaris were based on males to avoid interaction with reproductive processes, focusing on muscle and digestive gland tissues. This study intends to evaluate the effect of two fresh diets, a fish diet (bogue Boops boops) and a mixed diet (600 g kg−1 crab Portunus pelagicus and 400 g kg−1 bogue), on the biochemica...

Reliable reproduction and sufficient amounts of juveniles obtained in captivity constitute a main bottleneck for mass production of greater amberjack. The aim of the present study was to combine several aspects of broodstock management and hormonal induction, to obtain a large number of high quality spawns. Captured Seriola dumerili sub-adults were...

Two females bearing oocytes larger than 500μ and four fluent males were selected for injection of 20 μg.kg-1 GnRHa (Sigma-Aldrich Co. St. Louis, Missouri, USA). Broodstock, kept in 10 m3 tanks, under natural temperature and photoperiod conditions in the Canary Islands produced a high spawn quality: 92.28% fertilized eggs, 55.48% viable eggs, 77.76%...

El objetivo del presente estudio fue la obtención de puestas viables mediante inducción con GnRHa, de reproductores, capturados en el medio natural y mantenidos en condiciones de cultivo de S. rivoliana, y de la determinación de la calidad de las mismas.

Two females bearing oocytes larger than 500μ and four fluent males were selected for induction with successive injections of 20 μg.kg-1 GnRHa (Sigma-Aldrich Co. St. Louis, Missouri, USA). Broodstock kept in 10 m3 tanks, under natural temperature and photoperiod conditions in the Canary Islands. A total of twenty-two spawns were obtained with fiftee...

Two females with oocytes bigger than 500μ, and four fluent males, originally captured from the wild and acclimated to the broodstock of the GIA were used for this study. Fish were acclimated in tanks of 10 m3, and they were injected three times per month (June till November) with 20μg.kg-1 of GnRha. A total of 33 spawns with 15 injections were obta...

El objetivo del presente estudio fue la obtención de puestas viables, mediante inducción con GnRHa, de reproductores, capturados en el medio natural y mantenidos en condiciones de cultivo de S. dumerili, y de la determinación de su producción

Two females with oocytes bigger than 500μ, and four fluent males, were acclimated in a tank of 10 m3, and were injected three times per month (June till November) with 20μg.kg-1 of GnRha. The number of eggs obtained ranged from a minimum of 1,680,500 in June and a máximum of 6,699,000 in September, and the total was of 20,189,700. A positive relati...