Javier Parra

Productos Citrosol S.A. · Department of Postharvest Technology

Bioconjugate preparation is a fundamental step for antibody generation and immunoassay development to small chemical compounds. For analytical targets holding in their structure an aryl halogen atom, cross-coupling reactions may be a simple and efficient way to obtain functionalized derivatives; thus offering great potential to elicit robust and se...

Carbon nanotubes (CNTs) are nanomaterials with interesting emerging applications. Their properties makes CNTs excellent candidates for use as new nanovehicles in drug delivery, immunization and diagnostics. In the current study, we assessed the immune-response-amplifying properties of CNTs to haptens by using azoxystrobin, the first developed strob...

Potassium sorbate increases citrus weight loss on postharvest treatments while decay control efficacy when added to wax is poor

Picoxystrobin is a new generation fungicide primarily developed to be used in cereal crops. In the present study a novel collection of specific monoclonal antibodies has been produced using different immunizing haptens based on a carboxy-functionalized identical spacer arm attached to alternative positions of the target pesticide molecule. Two comp...

Azoxystrobin is a modern strobilurin fungicide used around the world to combat prime diseases affecting highly valuable crops. Accordingly, residues of this chemical are frequently found in food, even though mostly under maximum tolerated levels. We herein describe the development of an indirect competitive immunoassay for the determination of azox...

The relevance of the linker tethering site in haptens was investigated for antibody generation and immunoassay development. Three derivatives of the strobilurin fungicide picoxystrobin were synthesized with the same functionalized spacer arm located at three different positions. Protein conjugates of those haptens were employed as immunogens, and n...

The use of agrochemicals for crop protection may result in the presence of toxic residues in soils and aquatic environments, besides in foodstuffs. Most often just the parent compound is included in the definition of pesticide residue, even though chemicals resulting from biotransformation and degradation routes might also be of toxicological relev...

Amplificación de la respuesta inmune hacia haptenos empleando conjugados con nanotubos de carbono. Estudio de la relación entre el tamaño y forma de los nanotubos y sus propiedades inmunogénicas

The immune response to regioisomeric haptens of azoxystrobin with varied derivatization sites was studied. Based on the Sonogashira and Suzuki–Miyaura couplings and following a straightforward modular design, we have synthesized four haptens with the same linker anchored through C–C bonds and located at different sites of the molecule. The most ste...

This paper describes the original synthesis of a functionalized derivative of the fungicide picoxystrobin and the generation of the first reported monoclonal antibodies against this strobilurin pesticide. The synthetic hapten was prepared by total synthesis from commercial chemicals and incorporating the spacer arm through a carbon-carbon single bo...

Click-like synthesis of haptens with carboxylic spacer arms to be used in the production of monoclonal antibodies against azoxistrobin

[2-Percarboxyethyl]-functionalized silica reacts with ketones in supercritical carbon dioxide at 250 bar and 40 °C under flow conditions to yield the corresponding esters and lactones. The solid reagent can be easily recycled through treatment with 70% hydrogen peroxide in the presence of an acid at 0 °C. This procedure not only simplifies the isol...

Síntesis de haptenos de picoxistrobin: produccion de anticuerpos

Towards the development of a monoclonal immunoassay to azoxystrobin: synthesis and evaluation of immunizing haptens

Síntesis de análogos de azoxistrobin con brazo espaciador ácido como haptenos para la produccion de anticuerpos monoclonales