Javier Montero-Pau

Javier Montero-Pau
University of Valencia | UV · Instituto Cavanilles de Biodiversidad y Biología Evolutiva

PhD

About

66
Publications
13,252
Reads
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1,218
Citations
Citations since 2017
50 Research Items
929 Citations
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Additional affiliations
September 2018 - present
University of Valencia
Position
  • Professor (Assistant)
February 2014 - August 2014
University of Valencia
Position
  • PostDoc Position
January 2012 - August 2018

Publications

Publications (66)
Article
Full-text available
The emergence of reproductive isolation is key in maintaining within- and between-species diversity and one of the initial steps of speciation. In the Iberian Peninsula, the diverging populations of the Brachionus plicatilis rotifer create an ideal system to shed light on the mechanisms that give rise to the emergence of reproductive isolation. Her...
Article
Full-text available
A comprehensive collection of 1254 tomato accessions, corresponding to European traditional and modern varieties, early domesticated varieties, and wild relatives, was analyzed by genotyping by sequencing. A continuous genetic gradient between the traditional and modern varieties was observed. European traditional tomatoes displayed very low geneti...
Article
Full-text available
The development of reproductive barriers promotes within-species divergence and is a requisite for speciation to occur. Mate recognition in the rotifer B. plicatilis is mediated through a surface glycoprotein called Mating Recognition Protein (MRP). Here we investigate the genetic variation of the mmr-b, MRP coding, gene in different natural popula...
Article
Full-text available
A novel haplotype-based approach that uses Procrustes analysis and automatic classification was used to provide further insights into tomato history and domestication. Agrarian societies domesticated species of interest by introducing complex genetic modifications. For tomatoes, two species, one of which had two botanical varieties, are thought to...
Article
Full-text available
Tomato leaf curl New Delhi virus (ToLCNDV) emerged in the Mediterranean Basin in 2012 as the first DNA bipartite begomovirus ( Geminiviridae family), causing severe yield and economic losses in cucurbit crops. A major resistance locus was identified in the wild melon accession WM-7 ( Cucumis melo kachri group), but the mechanisms involved in the re...
Chapter
In order to provide a citizen education in Environmental Health, it is necessary to know the current educational scenario and also to give functional meaning to what is understood by competence in Environmental Health. This analysis requires exploring the coherence between: a) the health and environmental problems presented; b) the teaching offered...
Preprint
Full-text available
A comprehensive collection of 1,254 tomato accessions corresponding to European heirlooms and landraces, together with modern varieties, early domesticates and wild relatives, were analyzed by genotyping by sequencing. A continuous genetic gradient between the vintage and modern varieties was observed. European vintage tomatoes displayed very low g...
Article
Full-text available
Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) play an important role in a variety of plant biological processes including growth, stress response, morphogenesis, signalling and cell wall biosynthesis. The GPI-anchor contains a lipid-linked glycan backbone that is synthesized in the endoplasmic reticulum (ER) where it is subsequentl...
Preprint
Full-text available
A novel haplotype-based approach that uses Procrustes analysis and automatic classification was used to provide further insights into tomato history and domestication. Agrarian cultures domesticated species of interest by introducing complex genetic modifications. For tomato, two species, and for one of them two botanical varieties: are thought to...
Article
Full-text available
Background: Summer squash (Cucurbita pepo: Cucurbitaceae) are a popular horticultural crop for which there is insufficient genomic and transcriptomic information. Gene expression atlases are crucial for the identification of genes expressed in different tissues at various plant developmental stages. Here, we present the first comprehensive gene exp...
Article
Full-text available
Trichomes are a common morphological defense against pests, in particular, type IV glandular trichomes have been associated with resistance against different invertebrates. Cultivated tomatoes usually lack or have a very low density of type IV trichomes. Therefore, for sustainable management of this crop, breeding programs could incorporate some na...
Preprint
Full-text available
Trichomes are a common morphological defense against pests, in particular, type IV glandular trichomes have been associated with resistance against different invertebrates. Cultivated tomatoes usually lack or have a very low density of type IV trichomes. Thus, specific breeding programs to incorporate these natural defences, that are common within...
Article
Full-text available
A collection of 163 accessions, including Solanum pimpinellifolium, Solanum lycopersicum var. cerasiforme and Solanum lycopersicum var. lycopersicum, was selected to represent the genetic and morphological variability of tomato at its centers of origin and domestication: Andean regions of Peru and Ecuador and Mesoamerica. The collection is enriched...
Article
Full-text available
This paper focuses on the competencies in environmental health acquired by students during compulsory education. Questionnaires addressing environmental health problems were completed by 923 students of primary and secondary schools from five different Spanish regions. The results for five challenging situations related to hunger, consumerism, clim...
Article
Full-text available
The early secretory pathway involves bidirectional transport between the endoplasmic reticulum (ER) and the Golgi apparatus and is mediated by coat protein complex I (COPI)-coated and coat protein complex II (COPII)-coated vesicles. COPII vesicles are involved in ER to Golgi transport meanwhile COPI vesicles mediate intra-Golgi transport and retrog...
Article
Full-text available
Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite whitefly transmitted begomovirus, responsible since 2013 of severe damages in cucurbit crops in Southeastern Spain. Zucchini (Cucurbita pepo) is the most affected species, but melon (Cucumis melo) and cucumber (Cucumis sativus) are also highly damaged by the infection. The virus has spread a...
Article
Full-text available
Cucurbita pepo contains two cultivated subspecies each of which encompasses four fruit-shape morphotypes (cultivar-groups). The Pumpkin, Vegetable Marrow, Cocozelle, and Zucchini Groups are of subsp. pepo and the Acorn, Crookneck, Scallop, and Straightneck Groups are of subsp. ovifera. Recently, a de novo assembly of the C. pepo subsp. pepo Zucchin...
Preprint
Full-text available
Plant's exquisite variation in floral traits at a macroevolutionary level is often interpreted as the result of adaptations to pollinators. However, field studies measuring pollinator-mediated evolution of flowers often find little evidence for strong selection. A possible explanation is the prevalence of periods of stasis, when selection on flower...
Article
Full-text available
Populations of passively dispersed organisms in continental aquatic habitats typically show high levels of neutral genetic differentiation despite their high dispersal capabilities. Several evolutionary factors, including founder events, local adaptation, and life cycle features such as high population growth rates and the presence of propagule ban...
Preprint
Full-text available
Populations of passively dispersed organisms in continental aquatic habitats typically show high levels of neutral genetic differentiation, despite their high dispersal capabilities. Several evolutionary factors, including founder events and local adaptation, and life cycle features such as high population growth rates and the presence of propagule...
Preprint
Populations of passively dispersed organisms in continental aquatic habitats typically show high levels of neutral genetic differentiation, despite their high dispersal capabilities. Several evolutionary factors, including founder events and local adaptation, and life cycle features such as high population growth rates and the presence of propagule...
Article
Full-text available
Objective: The current Organic Law for the Improvement of Educational Quality (LOMCE) incorporates substantial changes from the previous law, especially a new curricular configuration with a higher prevalence of optionality, which may affect the development of health competencies by students. The objective was to evaluate the treatment of Health E...
Article
Full-text available
Background: The current Organic Law for the Improvement of Educational Quality (LOMCE) incorporates substantial changes from the previous law, in particular a new curricular configuration with a higher prevalence of optionality, which may affect the development of health competencies by students. The objective was to address health education in the...
Data
Figure S2 Distribution of N50 for contigs (A) and scaffolds (B) for different k‐mer size values.
Data
Figure S4 Summary of the structural annotation of C. pepo genome.
Data
Table S3 Scaffolds of genome assembly v.3.2. containing chloroplastic and mitochondrial regions. The pseudochromosomes were built from the version 3.2 scaffolds.
Data
Table S8 List of genes that are duplicated in Cucurbita pepo. Predicted functions are also shown.
Data
Figure S5 Transcriptome GO annotation statistics A) by levels and B) at level 6.
Data
Figure S6 Genome GO annotation statistics (A) by levels and (B) at level 6.
Data
Table S1 Accessions of domesticated and wild Cucurbita spp. used for transcriptomic and phylogenetic analyses. The number of reads used for assembly the transcriptomes, and number of genes and transcripts obtained are also shown.
Data
Table S2 NGS library statistics. Numbers of raw reads, percentage of nucleotides over 30 quality, coverage, % of reads filtered out during the cleaning process, % of reads without adaptor, % of chimeric reads, number of cleaned reads, coverage of cleaned reads, and percentage of nucleotides over 30 quality in the clean reads.
Data
Table S4 Genome v.4.1 pseudochromosome configuration. The order, orientation and size of genome v. 3.2 scaffolds grouped in each pseudochromosome is shown. Equivalence of pseudochromosomes and linkage groups of Montero‐Pau et al. (2017) genetic map is also shown.
Data
Table S5 Summary of repetitive elements found in Cucurbita pepo, Cucumis melo, Cucumis sativus and Citrullus lanatus. All results are expressed in bp.
Data
Table S9 List of genes that are duplicated in Cucurbita pepo but not in Cucumis melo, Cucumis sativus or Citrullus lanatus. Predicted functions are also shown.
Data
Figure S1 Distribution of sequences of k‐mer size 41 for different levels of coverage.
Data
Table S7 List of genes that are single copy in Cucurbita pepo. Predicted functions are also shown.
Data
Figure S3 Correlation between genetic and physical distances for each pseudochromosome. Color scale represents fraction of repetitive DNA.
Data
Figure S7 Repetitive elements. Fraction of the genome covered by different types of repetitive elements in C. pepo and four Cucurbita genomes (A). Maximum likelihood phylogenetic trees of C. pepo elements of Copia (B) and Gypsy (C) LTR superfamilies based on a fragment of the reverse transcriptase.
Data
Figure S8 Results of the GO enrichment test. Treemaps for the results of the GO enrichment tests on single‐copy genes in Cucurbita pepo, all duplicated genes in C. pepo and genes that are duplicated in C. pepo but not in other cucurbit genomes. The area of the rectangles represents the negative logarithm of the enrichment test FDR.
Data
Table S6 Gene family (orthogroups and paralogs in OrthoMCL) identification.
Data
Table S10 GO term enrichment tests. The results are shown for single‐copy genes in Cucurbita pepo, duplicated genes, and genes that are exclusively duplicated in C. pepo when compared with other cucurbit genomes.
Article
Full-text available
The Cucurbita genus (squashes, pumpkins and gourds) includes important domesticated species such as C. pepo, C. maxima and C. moschata. In this study, we present a high-quality draft of the zucchini (C. pepo) genome. The assembly has a size of 263 Mb, a scaffold N50 of 1.8 Mb, and 34,240 gene models. It includes 92% of the conserved BUSCO core gene...
Preprint
Full-text available
Populations of passively dispersed organisms in continental aquatic habitats typically show high levels of neutral genetic differentiation, despite their high dispersal capabilities. Several evolutionary factors, including founder events and local adaptation, and life cycle features such as high population growth rates and the presence of propagule...
Article
Full-text available
In recent decades, the use of molecular techniques in rotifers has revealed the existence of many cryptic species. Although strong competition is expected among cryptic species, these species are often sympatric. Here, we present a review of sympatric cryptic rotifer species, focusing on those cases in which niche differentiation has been investiga...
Article
Full-text available
Diapausing egg banks are reservoirs of ecological and genetic diversity in continental zooplankton. However, although habitat size has often been used as a proxy for population size, the relationship between diapausing egg bank size and genetic diversity has not been explicitly tested in zooplankton. We estimated the density and size of diapausing...
Conference Paper
Full-text available
Inquiry-based science education (IBSE) focuses on the development of science process skills. This teaching methodology has been shown to be especially effective during Primary School as it allows children to explore and confront their own ideas about Nature. Also, a methodology based on process skills is coherent with the main purpose of this educa...
Preprint
Full-text available
The Cucurbita genus (squashes, pumpkins, gourds) includes important domesticated species such as C. pepo, C. maxima and C. moschata. In this study, we present a high-quality draft of the zucchini (C. pepo) genome. The assembly has a size of 263 Mb, a scaffold N50 of 1.8 Mb, 34,240 gene models, includes 92% of the conserved BUSCO core gene set, and...
Article
Full-text available
Background Cucurbita pepo is a cucurbit with growing economic importance worldwide. Zucchini morphotype is the most important within this highly variable species. Recently, transcriptome and Simple Sequence Repeat (SSR)- and Single Nucleotide Polymorphism (SNP)-based medium density maps have been reported, however further genomic tools are needed f...
Conference Paper
Full-text available
Teaching science by inquiry allows students to develop scientific skills, which is key during primary school, and enhances learning. In this study we present the results of a survey to 63 primary school teachers in Spain about their practices in science class and their knowledge about inquiry-based science education (IBSE). Our results show that co...
Chapter
Cucurbita pepo is one of the main crops of the Cucurbitaceae family. Despite its agricultural and biological importance, genomic research on this species has started later and progresses slowerthan in other cucurbits. Here we review the latest genetic and genomic tools developed for C. pepo. A whole-genome shotgun strategy based on pair-end and mat...
Conference Paper
Full-text available
Este estudio analiza cómo es la enseñanza de las ciencias en educación primaria a través de entrevistas realizadas a maestros de diferentes cursos. El objetivo principal es diagnosticar el estado de la situación y entender qué espacio de mejora hay según los propios maestros. Finalmente, se pretende que reflexionen sobre la necesidad de una enseñan...
Article
Full-text available
Competition for resources can lead to species exclusion. However, this exclusion may be avoided if species show differential adaptation to physical environment. Empirical studies on competition are difficult when species are phylogenetically close and have complex life cycles. This is the case of B. plicatilis and B. manjavacas, two cryptic rotifer...
Article
Trade-offs are important in life-history evolution and coexistence of competitors. However, how alternative life-history optima might promote competitor coexistence has received little attention. The rotifers Brachionus plicatilis and Brachionus manjavacas are two cryptic species with a considerable ecological overlap in relation to biotic factors....
Article
Full-text available
Life-history traits may have an important role in promoting species coexistence. However, the complexity of certain life cycles makes it difficult to draw conclusions about the conditions for coexistence or exclusion based on the study of short-term competitive dynamics. Brachionus plicatilis and B. manjavacasare two cryptic rotifer species co-occu...
Article
Full-text available
Background: Domestication modifies the genomic variation of species. Quantifying this variation provides insights into the domestication process, facilitates the management of resources used by breeders and germplasm centers, and enables the design of experiments to associate traits with genes. We described and analyzed the genetic diversity of 1,...
Article
The evolution of specialist and generalist strategies is a central topic in ecology with strong implications for the biodiversity and structure of communities. Environmental unpredictability has been suggested as a key factor affecting the relative advantages of generalist species. However, life cycle features, like diapause, can also play a major...
Article
Full-text available
The intrinsic rate of population increase (r) is a common performance measure in many ecological and evolutionary studies. However, in life cycles with diapause investment resources are split into a short-term (current population growth) and a long-term (population survival through periods of unsuitable habitat conditions) component, which complica...
Article
Full-text available
Co-occurrence of cryptic species raises theoretically relevant questions regarding their coexistence and ecological similarity. Given their great morphological similitude and close phylogenetic relationship (i.e., niche retention), these species will have similar ecological requirements and are expected to have strong competitive interactions. This...
Article
Full-text available
Despite their high morphological similarity, cryptic species often coexist in aquatic habitats presenting a challenge in the framework of niche differentiation theory and coexistence mechanisms. Here we use a rotifer species complex inhabiting highly unpredictable and fluctuating salt lakes to gain insights into the mechanisms involved in stable co...
Article
Full-text available
The increasing evidence of coexistence of cryptic species with no recognized niche differentiation has called attention to mechanisms reducing competition that are not based on niche-differentiation. Only sex-based mechanisms have been shown to create the negative feedback needed for stable coexistence of competitors with completely overlapping nic...
Article
Full-text available
Sexual reproduction is density-dependent in several cyclically parthenogenetic rotifer species and induced by a chemical signal produced by the rotifers. As population density increases, the chemical accumulates in the medium until reaching a threshold that triggers sex. The density threshold for sex initiation is of ecological and evolutionary imp...
Article
There has been a substantial leap forward in the quantity and quality of genomic resources available for research on rotifers in recent years. We used available genomic and bioinformatics resources to identify variable regions of the genome to design PCR and sequencing primers for the Brachionus plicatilis complex. We then tested their suitability...
Article
1. Aquatic invertebrates display a wide array of alternative reproductive modes from apomixis to hermaphroditism and cyclical parthenogenesis. These have important effects on genetic diversity and population structure. Populations of the ‘living fossil’Triops cancriformis display a range of sex ratios, and various reproductive modes are thought to...
Article
We describe the application of a simple, low-cost, and effective method of DNA extraction (hot sodium hydroxide and Tris, HotSHOT) to the diapausing propagules of continental aquatic invertebrates for its use in PCR amplification. We illustrate the use of the technique in cladocerans, rotifers, anostracans, notostracans, and copepod diapausing eggs...
Article
Recent phylogeographical assessments have consistently shown that continental zooplankton display high levels of population subdivision, despite the high dispersal capacity of their diapausing propagules. As such, there is an apparent paradox between observed cosmopolitanism in the zooplankton that is associated with long-distance dispersal, and st...

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Projects (4)
Project
Salud Ambiental. Significado del concepto, competencias, presencia en el currículo de la enseñanza obligatoria, actividades de innovación.