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Introduction
Publications
Publications (157)
Key message:
A set of SNP markers associated to bunch compactness and related traits were identified in grapevine. Bunch compactness plays an important role in the sanitary status and perceived quality of table and wine grapes, being influenced by cultural practices and by environmental and genetic factors, which are mostly unknown. In this work,...
Grapevine cluster compactness has a clear impact on fruit quality and health status, as clusters with greater compactness are more susceptible to pests and diseases and ripen more asynchronously. Different parameters related to inflorescence and cluster architecture (length, width, branching, etc.), fruitfulness (number of berries, number of seeds)...
Bunch compactness (or density) is a grapevine specific trait that affects the commercial quality and sanitary status of wine and tablegrapes. Compact bunches are more susceptible to diverse pests and diseases such as Botrytis bunch rot and their berries ripen more heterogeneously, causing important economic losses through a reduction in crop yield...
Background: There are thousands of grapevine varieties that display a wide range of variation for traits like grape use (wine, table grape or both), color or ripening time, but little is known about their reproductive performance, especially flowering and fruitset (conversion from flower to fruit). Works focused at the study of these traits in grap...
Global viticulture has evolved following market trends, causing loss of cultivar diversity and traditional practices. In Montenegro, modern viticulture co-exists with a traditional viticulture that still maintains ancient practices and exploits local cultivars. As a result, this region provides a unique opportunity to explore processes increasing g...
Precise identification is crucial to study and for the valorisation of ancient grapevine varieties, with many synonyms and misidentifications hampering this task. SSR-Multiplex PCR and SNP genotyping combined with HRM were used, to identify 19 varieties present in an ancient Portuguese vineyard. The combination of the methodologies allowed the iden...
Authentication of grapevine cultivars, Vitis vinifera L., is difficult, especially when analytical specimens lack diagnostic ampelographic characters, which prevents the verification of traceability systems aimed at guaranteeing varietal integrity. This issue is problematic when viticultural strategies and regulations associated with high-value win...
Thanks to current advances in sequencing technologies, novel bioinformatics tools, and efficient modelling solutions, association mapping has become a widely accepted approach to unravel the link between genotype and phenotype diversity in numerous crops. In grapevine, this strategy has been used in the last decades to understand the genetic basis...
La apirenia de la mayoría de las variedades comerciales de la vid (Vitis vinifera L.)
procede de 'Sultanina', una variedad con origen en Asia Menor. El principal objetivo de este trabajo ha sido la caracterización de posibles fuentes alternativas de apirenia en el germoplasma armenio. Se han estudiado 40 accesiones apirenas de las colecciones armen...
Seedlessness is a highly valuable trait in the EU and global grape markets. Genotyping and phenotyping of seedless varieties are an efficient tool for assessing the genetic diversity, determining the type of seedlessness, and optimizing their use in table breeding programs. In this work, a total of 42 Armenian Vitis vinifera L. seedless grapevine a...
Grapevines are challenged by a range of diseases and pests, causing economic losses and requiring often costly approaches to mitigate damage. Public interest in reducing the use of chemicals is a related challenge, along with climate change. Yet, the Vitis gene pool provides vast resources for the development of genetic resistance in rootstock and...
More than 100 grapevine varieties are registered as suitable for wine production in “Douro” and “Trás-os-Montes” Protected Designations of Origin regions; however, only a few are actually used for winemaking. The identification of varieties cultivated in past times can be an important step to take advantage of all the potential of these regions gra...
The South Caucasus is recognised as the primary Vitis vinifera L. (grapevine) domestication centre and has a high diversity of wild and cultivated grapevines. Archaeological findings indicate that winemaking activities have existed in Armenia for more than 6,000 years, viticulture being one of the most important activities of the modern Armenian ag...
Grapevine cultivars are clonally propagated to preserve their varietal attributes. However, genetic variations accumulate due to the occurrence of somatic mutations. This process is anthropically influenced through plant transportation, clonal propagation and selection. Malbec is a cultivar that is well-appreciated for the elaboration of red wine....
Grapevine is one of the most valuable fruit crops in the world. Adverse environmental conditions reduce fruit quality and crop yield, so understanding the genetic and molecular mechanisms determining crop yield components is essential to optimize grape production. The analysis of a diverse collection of grapevine cultivars allowed us to evaluate th...
BACKGROUND: Monoterpenes and C13-norisoprenoids are key terpenoid compounds for wine aroma. The enzyme encoded by VviDXS1 participates in terpenoid biosynthesis in grapevine fruits and gain-of-function mutations in this gene lead to characteristic muscat aroma. OBJECTIVE: To assess for VviDXS1 contribution to aroma variation in Northwestern Iberian...
Grapevine ( Vitis vinifera L.) cultivars are clonally propagated to preserve their varietal attributes. However, novel genetic variation still accumulates due to somatic mutations. Aiming to study the potential impact of clonal propagation history on grapevines intra-cultivar genetic diversity, we have focused on ‘Malbec’. This cultivar is apprecia...
Cluster compactness is a trait with high agronomic relevance, affecting crop yield and grape composition. Rachis architecture is a major component of cluster compactness determinism, and is a target trait toward the breeding of grapevine varieties less susceptible to pests and diseases. Although its genetic basis is scarcely understood, a prelimina...
The domesticated grapevine spread along the Mediterranean basin from the primary Near East domestication area, where the greatest genetic diversity is found in its ancestor, the wild vine populations. Portuguese wild populations are on the southwestern fringe of the distribution of the Vitis vinifera L. ssp. sylvestris (C.C. Gmel.) Hegi in Europe....
Paradoxically, continuous vegetative multiplication of traditional grapevine cultivars aimed to maintain cultivar attributes in this highly heterozygous species ends in the accumulation of considerable somatic variation. This variation has long contributed to cultivar adaptation and evolution under changing environmental and cultivation conditions...
Paradoxically, continuous vegetative multiplication of traditional grapevine cultivars aimed to maintain cultivar attributes in this highly heterozygous species ends in the accumulation of considerable somatic variation. This variation has long contributed to cultivar adaptation and evolution under changing environmental and cultivation conditions...
Previous studies showed that the number of berries is a major component of the compactness level of the grapevine clusters. Variation in number of fruits is regulated by events occurring in the fruitset, but also before during the flower formation and pollination, through factors like the initial number of flowers or the gametic viability. Therefor...
List of significantly differentially expressed genes (twofold ratio, p-value < 0.05) between at least two clones at E-L 18–19 or E-L 26.
List of variant polymorphisms between clones. Dark color: homozygous SNP, light color: heterozygous SNP. White: unclear polymorphism.
A) Principal component analysis at E-L 18–19 with the differentially expressed genes of all replicates of the four Tempranillo clones. B) Principal component analysis at E-L 26 with the differentially expressed genes of all replicates of the four Tempranillo clones.
Clustering of the differentially expressed genes at E-L 26 using QT+HCL method with a threshold of 0.2. Red background: lower expression in VP11, blue: higher expression in VP25 vs. compact clones. Cluster 14 corresponds to leftover genes that did not fit any of the profiles.
Clustering of the differentially expressed genes at E-L 18–19 using QT+HCL method with a threshold of 0.2. Red background: lower expression in VP11, green higher expression in VP11, blue: higher expression in VP25 vs. compact clones. Cluster 30 corresponds to leftover genes that did not fit any of the profiles.
Todavía hoy, algunas variedades blancas de vid
tienen un origen incierto y siguen siendo objeto
de estudio para muchos enólogos, botánicos y
genetistas. Se puede decir que hay dos mecanismos
fundamentales que pueden dar lugar a
la aparición de nuevas variedades de uva blanca:
1) la vía sexual, tras el cruzamiento de
parentales portadores de determi...
Vitis vinifera L. Bobal blanco no constituye una variedad genuina. Según los resultados de nuestra investigación, cuando se emplea el nombre de Bobal Blanco realmente se está empleando un sinónimo de Airén y en ningún caso se refiere a una mutación originaria de la variedad tinta Bobal.
Este libro se compone de dos partes, la primera parte introductoria, se desarrollan en tres capítulos. El primer capítulo describe los profundos cambios que se han producido en la viticultura gallega en los últimos 30 años. El segundo capítulo aborda el origen y las relaciones de parentescos de las variedades de cultivo tradicional en Galicia. El t...
El presente trabajo se ha centrado sobre la Cuadrilla de Ayala (Álava), donde se han localizado ocho poblaciones relictas de vid silvestre en diversos hábitats. Se ha comprobado su carácter dioico durante el periodo de floración. Se han identificado las principales especies parásitas existentes sobre estas parras y las principales componentes de su...
Seedlessness is greatly prized by consumers of fresh grapes. While stenospermocarpic seed abortion determined by the SEED DEVELOPMENT INHIBITOR (SDI) locus is the usual source of seedlessness in commercial grapevine (Vitis vinifera) cultivars, the underlying sdi mutation remains unknown. Here, we undertook an integrative approach to identify the ca...
Grapevine is a very important crop species that is mainly cultivated worldwide for fruits, wine and juice. Identification of the genetic bases of performance traits through association mapping studies requires a precise knowledge of the available diversity and how this diversity is structured and varies across the whole genome. An 18k SNP genotypin...
Eurasian wild grapewine, a threatened phytogenetic resource bound to humanity history.
The present article, focused on the Eurasian wild grapevine, the dioecious parental of the present cultivars, shows the ancient uses of this vine in the past and its importance as a plant genetic resource in the genetic
improvement of existing varieties and to r...
Background
Low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. It is usually evaluated by the quantification of the number of viable and non-viable pollen grains that are present in a sample after an adequate pollen grain staining procedure. Although the manual counting of both...
Grape color somatic variants that can be used to develop new grapevine cultivars occasionally appear associated to deletion events of uncertain origin. To understand the mutational mechanisms generating somatic structural variation in grapevine, we compared the Tempranillo Blanco (TB) white berry somatic variant to its black berry ancestor, Tempran...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to cell cycle. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all comparisons are in...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to plant–pathogen interaction. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all co...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to ethylene signaling. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all comparison...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to regulation of actin cytoskeleton. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to phenylpropanoids biosynthesis. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to flavonoids biosynthesis. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all compa...
Phenotypic data and pair-wise t-tests of the selected clones during three seasons.
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to R proteins from plant–pathogen interaction. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to cell wall metabolism. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all comparis...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to anthocyanin biosynthesis. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all comp...
Adapted Cytoscape networks including transcript differentially expressed in flowers between loose and compact clones related to ethylene signaling. Genes over-expressed in compact clones in all comparisons are in dark red. Genes over-expressed in compact clones in 2 or 3 comparisons are in red. Genes over-expressed in loose clones in all comparison...
Gene expression values data. Sheet 1: normalized data for each samples, value are expressed as log2 of intensity. Sheet 2: functional annotation. Sheet 3: significance of the differential expression, ratio of transcripts expression in each comparison, genes meeting the cutoff of fold change >2 and P < 0.05 are reported as 1 for compact clones and −...
Background and Aims: Grapevine cultivation has a long tradition in Portugal. Presently, 343 cultivars are legally accepted for wine production. Two hundred and eighty eight accessions from the Portuguese National Ampelographic Collection (including 27 wild grapevines) were genotyped using single-nucleotide polymorphisms (SNP) and compared with the...
Core collections are nowadays widely employed in diverse studies on plant genetics. The more extensively used method to build core collections (maximization strategy) is based on the selection, from a global collection, of those accessions which maximize the number of different alleles and phenotypic classes (classes’ richness). However, different...
BACKGROUND: Grapevine cluster morphology influences the quality and commercial value of wine and table grapes. It is routinely evaluated by subjective and inaccurate methods that do not meet the requirements set by the food industry. Novel 2D and 3D machine vision technologies emerge as promising tools for its automatic and fast evaluation.
RESULTS...
Abstract Spain has a very old viticulture history, and it is the country with the largest vineyard surface in the world. This chapter reviews the grapevine genetic improvement efforts made in Spain, which have mainly followed two alternative routes, depending on the grape use. Wine varieties have been improved through clonal selection, while there...
Background:
Domestication and selection of Vitis vinifera L. for table and wine grapes has led to a large level of berry size diversity in current grapevine cultivars. Identifying the genetic basis for this natural variation is paramount both for breeding programs and for elucidating which genes contributed to crop evolution during domestication a...
Seedlessness is a relevant trait in grapevine cultivars intended for fresh consumption or raisin production. Previous DNA marker analysis indicated that Corinto bianco (CB) is a parthenocarpic somatic variant of the seeded cultivar Pedro Ximenes (PX). This study compared both variant lines to determine the basis of this parthenocarpic phenotype. At...
Background and AimsGrapevine bunch compactness is an important trait with impact on fruit quality, mainly affecting the susceptibility to bunch rot. Many and different variables have been reported to have a significant influence on the variation of bunch compactness in particular cultivars, but little is known about the role of such variables in a...
Background and AimsBunch compactness is a key feature determining grape and wine composition because tight bunches show a less homogeneous ripening, and are prone to greater fungal disease incidence. The Organisation Internationale de la Vigne et du Vin descriptor, the most recent method for the assessment of bunch compactness, requires visual insp...
The use of Single Nucleotide Polymorphism (SNP) markers allows genetic identification and parentage analysis of grapevine cultivars. Ancient cultivars like 'Hebén' were widely cultivated in the Iberian Peninsula along its history and likely contributed to the origin of varieties which are still cultivated today. The goal of our study was to search...
The grapevine cultivar known in Portugal as 'Al-frocheiro', and in Spain as 'Bastardo Negro', 'Bruñal' or 'Baboso Negro', plays a central role in the genetic network of the Iberian Peninsula grapevine cultivars. Three sets of different molecular markers, SNPs, nS-SRs and cpSSRs, revealed more than twenty parent-offspring links with this cultivar. '...
The Near East and the Caucasus regions are considered as gene and domestication centre for grapevine. In an earlier project "Conservation and Sustainable Use of Grapevine Genetic Resources in the Caucasus and Northern Black Sea Region" (2003-2007) it turned out that 2,654 accessions from autochthonous cultivars maintained by Armenia, Azerbaijan, Ge...