Jatin G Burniston

Jatin G Burniston
Liverpool John Moores University | LJMU · Research Institute for Sport and Exercise Sciences (RISES); Liverpool Centre for Cardiovascular Science (LCCS)

35.41
 · 
BSc (Hons), PhD, FECSS

About

97
Publications
9,327
Reads
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1,655
Citations
Introduction
CALL FOR PAPERS :: Striated Muscle Proteomics Vol 2 :: https://www.mdpi.com/journal/proteomes/special_issues/muscle_proteomics :: :: Please consider submitting an article if you have cardiac or skeletal muscle proteomics data.
Research Experience
September 2010 - present
Liverpool John Moores University
Position
  • Programme Leader
Description
  • Programme Leader MSc Sport & Exercise Physiology
January 2002 - December 2012
Liverpool John Moores University
Position
Education
November 2000 - November 2003
Liverpool John Moores University
Field of study
  • Muscle Physiology
July 1997 - September 2000
University of Portsmouth
Field of study
  • Sport & Exercise Sciences

Publications

Publications (97)
Article
Full-text available
Endurance exercise improves cardiac performance and affords protection against cardiovascular diseases but the signalling events that mediate these benefits are largely unexplored. Phosphorylation is an widely studied post-translational modification involved in intracellular signalling, and to discover novel phosphorylation events associated with e...
Article
It is generally accepted that muscle adaptation to resistance exercise (REX) training is underpinned by contraction-induced, increased rates of protein synthesis and dietary protein availability. By using dynamic proteome profiling (DPP), we investigated the contribution of both synthesis and breakdown to changes in abundance on a protein-by-protei...
Chapter
Full-text available
Proteomic investigations aim to achieve broad-scale characterisation of the protein complement of muscle and also perform non-targeted differential analysis of the muscle proteome under different conditions (e.g. health versus disease). In the majority, proteomic studies have generated new insight by linking patterns of protein abundance or post-tr...
Article
We investigated the repeatability of dynamic proteome profiling (DPP), which is a novel technique for measuring the relative abundance (ABD) and fractional synthesis rate (FSR) of proteins in humans. LC‐MS analysis was performed on muscle samples taken from male participants (n = 4) that consumed 4 × 50 ml doses of deuterium oxide (2H2O) per day fo...
Article
Full-text available
Muscle adaptations to exercise are underpinned by alterations to the abundance of individual proteins, which may occur through a change either to the synthesis or degradation of each protein. We used deuterium oxide (2H2O) labelling and chronic low-frequency stimulation (CLFS) in vivo to investigate the synthesis, abundance and degradation of indiv...
Article
Full-text available
Differences in the protein composition of fast- and slow-twitch muscle may be maintained by different rates of protein turnover. We investigated protein turnover rates in slow-twitch soleus and fast-twitch plantaris of male Wistar rats (body weight 412 ± 69 g). Animals were assigned to four groups (n = 3, in each), including a control group (0 d) a...
Article
This study aimed to develop a method for measuring the abundance and synthesis rate of human muscle proteins in molar rather than fractional (%/d) terms. Losses in proteostasis are implicated in ageing and chronic diseases but research is hindered by a lack of methods for studying protein dynamics in humans. Protein‐specific fractional synthesis ra...
Article
Physical activity increases muscle protein synthesis rates. However, the impact of exercise on the coordinated up- and/or downregulation of individual protein synthesis rates in skeletal muscle tissue remains unclear. The authors assessed the impact of exercise on mixed muscle, myofibrillar, and mitochondrial protein synthesis rates as well as indi...
Conference Paper
Full-text available
Adult muscle consists of multinucleated myofibers that originate from myogenic precursor cells during embryonic development. The gene programme of muscle differentiation is well described, but currently there is paucity of data regarding protein synthetic responses during muscle cell fusion. We used stable isotope (deuterium oxide, D2O) labelling o...
Article
Full-text available
Bi-directional selection for either high- or low-responsiveness to endurance running has created divergent rat phenotypes of high-response trainers (HRT) and low-response trainers (LRT). We conducted proteome profiling of HRT and LRT gastrocnemius of 10 female rats (body weight 279 ± 35 g; n=5 LRT and n=5 HRT) from generation 8 of selection. Differ...
Research
Full-text available
The lack of standardisation of methods and procedures have hindered agreement in the literature related to time-of-day effects on repeated sprint performance and needs clarification. Therefore, the aim of the present study was to investigate and systematically review the evidence relating to time-of-day based on performance measures in repeated-spr...
Book
This book is a collection of principles and current practices in omics research, applied to skeletal muscle physiology and disorders. The various sections are categorized according to the level of biological organization, namely, genomics (DNA), transcriptomics (RNA), proteomics (protein), and metabolomics (metabolite). With skeletal muscle as the...
Preprint
Full-text available
There has been an increase in the number of publications related to time-of-day variation on repeated sprint performance, the exact mechanisms related to this observed diurnal variation in human performance are still, yet, unknown (Pullinger et al. 2014). In addition, the large differences between exercise protocols currently present in the current...
Article
Full-text available
Purpose:Investigate football-induced fatigue during hypoxia on RS and perceptual-cognitive skills. Methods: Ten semi-professional footballers underwent a control session (0-m) to quantify RS in a non-fatigued state; and three hypoxia sessions (0-m;1500-m;3000-m) examining RS and perceptual-cognitive skills for a given physical workload. The mean n...
Article
Full-text available
We have investigated the effects that sleep restriction (3-h sleep during two consecutive nights) have on an evening (17:00 h) submaximal weightlifting session; and whether this performance improves following a 1-h post-lunch powernap. Fifteen resistance-trained males participated in this study. Before the experimental protocol commenced, 1RM bench...
Article
The present study investigated the magnitude of diurnal variation in back squat and bench press using the MuscleLab linear encoder over three different loads and assessed the benefit of an active warm-up to establish whether diurnal variation could be negated. Ten resistance-trained males underwent (mean ± SD: age 21.0 ± 1.3 yrs, height 1.77 ± 0.06...
Article
We have investigated the magnitude of diurnal variation in back squat and bench press performance using the MuscleLab force velocity transducer. Thirty resistance-trained males (mean ± SD: age 21.7 ± 1.4 years; body mass 80.5 ± 4.5 kg; height 1.79 ± 0.06 m) underwent two sessions at different times of day: morning (M, 07:30 h) and evening (E, 17:30...
Article
Full-text available
The present study investigated whether increasing morning rectal temperatures (Trec) to evening levels, or increasing morning and evening Trec to an “optimal” level (38.5°C), resulting in increased muscle temperatures (Tm), would offset diurnal variation in repeated sprint (RS) performance in a causal manner. Twelve trained males underwent five ses...
Article
Full-text available
The present study investigated whether increasing morning rectal temperatures (Trec) to resting evening levels, or decreasing evening Trec or muscle (Tm) temperatures to morning values, would influence repeated sprint (RS) performance in a causal manner. Twelve trained males underwent five sessions [age (mean ± SD) 21.8 ± 2.6 yr, peak oxygen uptake...
Article
Full-text available
We performed a systematic review and meta-analysis of proteomics literature that reports human skeletal muscle responses in the context of either pathological decline associated with obesity/T2DM and physiological adaptations to exercise training. Literature was collected from PubMed and DOAJ databases following PRISMA guidelines using the search t...
Article
Full-text available
To investigate the feasibility of using isolated knee performance to measure the f-v profile of human muscle in vivo, we were using isokinetic dynamometry (IKD) technique that span the entire f-v profile of skeletal muscle. With institutional ethics approval, eleven healthy males (mean ± SD: age, 24.9 ± 3.1 years; body mass, 80.2 ± 10.1 kg; height,...
Article
Full-text available
Understanding how exercise causes muscles to adapt is fundamental to improving our health, quality-of-life and longevity. This article is protected by copyright. All rights reserved.
Conference Paper
Full-text available
Resistance exercise is a potent stimulus for muscle adaptation and involves selective changes in protein abundance. Our objective was to use Dynamic Proteome Profiling to calculate the contributions of synthesis and degradation to changes in abundance on a protein by protein basis. After 3 days of dietary control (65% Carbohydrate, 20% Fat, 15% Pro...
Article
Full-text available
Mechanical ventilation (MV) is a life-saving intervention for many critically ill patients. Unfortunately, prolonged MV results in the rapid development of diaphragmatic atrophy and weakness. Importantly, endurance exercise training results in a diaphragmatic phenotype that is protected against ventilator-induced diaphragmatic atrophy and weakness....
Data
Proteomic analysis of detergent soluble diaphragm muscle proteins. Permanent DOI: https://figshare.com/articles/JGB_Progensis_QIP_Diaphragm_analysis_xlsx/4272818/1. (XLSX)
Data
Proteomic analysis of mitochondrial enriched fraction of diaphragm muscle. Permanent DOI: https://figshare.com/articles/JGB_Progensis_QIP_Diaphragm_analysis_xlsx/4272818/1. (XLSX)
Article
Full-text available
Traditional methods for phenotyping skeletal muscle (e.g., immunohistochemistry) are labor-intensive and ill-suited to multixplex analysis, i.e., assays must be performed in a series. Addressing these concerns represents a largely unmet research need but more comprehensive parallel analysis of myofibrillar proteins could advance knowledge regarding...
Article
Full-text available
The turnover of muscle protein is responsive to different (patho)-physiological conditionsbut little is known about the rate of synthesis at the level of individual proteins or whether thisvaries between different muscles. We investigated the synthesis rate of eight proteins (actin, albumin,ATP synthase alpha, beta enolase, creatine kinase, myosin...
Poster
Full-text available
The turnover of muscle protein is responsive to different (patho)-physiological conditions but little is known about the rate of synthesis at the level of individual proteins or whether this varies between different muscles. We investigated the synthesis rate of 8 proteins (actin, albumin, ATP synthase alpha, beta enolase, creatine kinase, myosin e...
Article
Full-text available
Muscle force production and power output are greater in the evening than in the morning but it is not clear which aspect of muscle performance exhibits the greatest amount of diurnal variation. Here we investigate the magnitude of diurnal variation using a selection of different techniques that span the entire force–velocity profile of skeletal mus...
Conference Paper
Full-text available
To discover signalling events associated with acute exercise we investigated the cardiac phosphoproteome response to a standardised exercise test. Male Wistar rats (346 ± 18 g, n = 6 per group) performed a treadmill test to measure their VO2peak and were killed either immediately after or 3 h after cessation of the exercise; control rats did not pe...
Article
Full-text available
Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects w...
Article
Divergent selection has created rat phenotypes of high- and low-capacity runners (HCR and LCR, respectively) that have differences in aerobic capacity and correlated traits such as adiposity. We analysed visceral adipose tissue of HCR and LCR using label-free HDMSE profiling. The running capacity of HCR was 9-fold greater than LCR. Proteome profili...
Article
Full-text available
Ageing is associated with several physiological declines to both the cardiovascular (e.g. reduced aerobic capacity) and musculoskeletal system (muscle function and mass). Ageing may also impair the adaptive response of skeletal muscle mitochondria and redox-regulated stress responses to an acute exercise bout, at least in mice and rodents. This is...
Article
We report automated and time efficient (2 h per sample) profiling of muscle using ultra-performance liquid chromatography (LC) coupled directly with high-definition mass spectrometry (HDMSE). Soluble proteins extracted from rat gastrocnemius (n = 10) were digested with trypsin and analysed in duplicate using a 90 min RPLC gradient. Protein identifi...
Article
Background Obstructive sleep apnoea (OSA) is a common complication of obesity and can have a substantial negative impact on a patient's quality of life and risk of cardiovascular disease. The aim of this case-control study was to undertake discovery profiling of urinary peptides using capillary electrophoresis-mass spectrometry (CE-MS) in obese sub...
Article
Full-text available
Several redox-regulated responses to an acute exercise bout fail in aged animal skeletal muscle including the ability to up regulate the expression of antioxidant defence enzymes and Heat Shock Proteins (HSPs). These findings are generally derived from studies on sedentary rodent models and thus may be related to reduced physical activity and/or in...
Article
Full-text available
Unlabelled: Profiling of protein species is important because gene polymorphisms, splice variations and post-translational modifications may combine and give rise to multiple protein species that have different effects on cellular function. Two-dimensional gel electrophoresis is one of the most robust methods for differential analysis of protein s...
Article
Full-text available
While 2DGE offers robust comparative analysis of protein species, standard gel systems have limited ability to resolve proteins larger than ~150 kDa or proteins at the extremes of the pI scale (i.e., <pH 4 or >pH 9). Bottom-up workflows overcome these issues by digesting the sample into peptides, which have fewer solubility issues. Typically, pepti...
Article
Full-text available
In active males, muscle force production and short-term (<6 s) anaerobic performance are significantly greater in the evening compared with the morning. This diurnal variation is attributed to motivational, peripheral and central factors, and possibly the higher core and muscle temperatures observed in the evening. However, little is known regardin...
Article
Full-text available
Two-dimensional gel electrophoresis provides robust comparative analysis of skeletal muscle, but this technique is laborious and limited by its inability to resolve all proteins. In contrast, orthogonal separation by SDS-PAGE and reverse-phase liquid chromatography (RPLC) coupled to mass spectrometry (MS) affords deep mining of the muscle proteome,...
Article
Full-text available
Muscle force production and power output in active males, regardless of the site of measurement (hand, leg, or back), are higher in the evening than the morning. This diurnal variation is attributed to motivational, peripheral, and central factors and higher core and, possibly, muscle temperatures in the evening. This study investigated whether dec...
Article
Full-text available
Muscle force production and power output in active males, regardless of the site of measurement (hand, leg, or back), are higher in the evening than the morning. This diurnal variation is attributed to motivational, peripheral, and central factors and higher core and, possibly, muscle temperatures in the evening. This study investigated whether dec...
Article
Full-text available
Background: The aim of the present study was to determine the influence of age and habitual activity level, at rest and following a single bout of high-intensity exercise, on the levels of three proteins poly(ADP-ribose) polymerase-1 (PARP-1), cleaved-PARP-1 and poly(ADP-ribose) glycohydrolase (PARG), involved in the DNA repair and cell death respo...
Article
Few studies have investigated heterogeneity of selection response in replicate lines subjected to equivalent selection. We developed 4 replicate lines of mice based on high levels of voluntary wheel running (high runner or HR lines) while also maintaining 4 non-selected control lines. This led to the unexpected discovery of the HR mini-muscle (HR(m...
Article
Full-text available
The aim of the present study was to examine the effects of ageing and training status on (1) markers of skeletal muscle mitochondrial content and (2) the ability to activate the acute signalling pathways associated with regulating exercise-induced mitochondrial biogenesis. Muscle biopsies were obtained from the vastus lateralis muscle of young untr...
Article
Intramuscular triglyceride (IMTG) utilization is enhanced by endurance training (ET) and is linked to improved insulin sensitivity. This study first investigated the hypothesis that ET-induced increases in net IMTG breakdown and insulin sensitivity are related to increased expression of perilipin 2 (PLIN2) and perilipin 5 (PLIN5). Secondly, we hypo...
Article
The lipid droplet (LD)-associated protein perilipin 2 (PLIN2) appears to colocalize with LDs in human skeletal muscle fibres, although the function of PLIN2 in the regulation of intramuscular triglyceride (IMTG) metabolism is currently unknown. Here we investigated the hypothesis that the presence of PLIN2 in skeletal muscle LDs is related to IMTG...
Article
Selection on running capacity has created rat phenotypes of high-capacity runners (HCRs) that have enhanced cardiac function and low-capacity runners (LCRs) that exhibit risk factors of metabolic syndrome. We analysed hearts of HCRs and LCRs from generation 22 of selection using DIGE and identified proteins from MS database searches. The running ca...
Article
Full-text available
Regular exercise is effective in the prevention of chronic diseases and confers a lower risk of death in individuals displaying risk factors such as hypertension and dyslipidemia. Thus, knowledge of the molecular responses to exercise provides a valuable contrast for interpreting investigations of disease and can highlight novel therapeutic targets...
Conference Paper
Full-text available
Selection on running capacity has created rat phenotypes of high capacity runners (HCR) that have enhanced cardiac function and low capacity runners (LCR) that exhibit features of metabolic syndrome. Proteins that regress with differences in running capacity may be mechanistically responsible for this divergence in disease risk. Hearts of 6 HCR and...
Article
Full-text available
The expression, regulation and function of mammalian Hippo pathway members in skeletal muscle is largely unknown. The aim of this study was thus to test the hypothesis that core members of the mammalian Hippo pathway are expressed in skeletal muscle and that the transcriptional co-factor Yap, a core member of the Hippo pathway, regulates C2C12 myog...
Article
Full-text available
No previous study has used proteomics to investigate the effects of exercise training on human skeletal muscle. Five recreationally active men completed a 6-wk training programme involving three sessions per week, utilising six 1-min bouts at maximum oxygen uptake (V O(2)max) interspersed with 4 min at 50% V O(2)max. Vastus lateralis was biopsied a...
Article
Full-text available
This study investigates the effects of short-term endurance training on heat shock protein (HSP) adaptations of male and female human skeletal muscle. The data demonstrate that females did not respond to continuous or interval training in terms of increasing HSP content of the vastus lateralis muscle. In contrast, males displayed HSP adaptations to...
Article
Endurance training improves cardiac function and protects against heart disease. The rodent intensity-controlled running model replicates endurance exercise in humans and can be used to investigate molecular adaptations in the heart. Rats (n = 6, 280 +/- 3 g) performed exercise tests to measure their peak oxygen uptake (VO2peak) and training was pr...
Article
Proteomics investigations using 2-dimensional electrophoresis (2-DE) cannot resolve the entire cardiac proteome because some proteins, including myosin heavy chains (MyHC), are insoluble in the buffers required for isoelectric focusing. Here, we report an automated mass spectrometry (MS) method complementary to 2-DE and capable of yielding importan...
Article
The adaptation of skeletal muscle to endurance exercise has not previously been investigated using proteomic techniques. Such work could improve our understanding and generate novel information regarding the effects of exercise. Plantaris muscles were investigated from rats exercised on treadmills at 70-75% peak oxygen uptake (V O(2)peak) for 30 mi...