Jason Kroll

Jason Kroll
Vivaltes

Molecular and Cell Biology PhD

About

17
Publications
1,562
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
328
Citations
Citations since 2017
12 Research Items
220 Citations
201720182019202020212022202301020304050
201720182019202020212022202301020304050
201720182019202020212022202301020304050
201720182019202020212022202301020304050
Additional affiliations
October 2015 - July 2018
AMOLF
Position
  • PostDoc Position
Description
  • Stochastic cell fate decisions in C. elegans.
August 2011 - May 2015
University of California, Berkeley
Position
  • Instructor
Description
  • Genetic suppression of epilepsy and seizures in Drosophila.
July 2010 - July 2011
Janelia Farm Research Campus
Position
  • Technician
Description
  • Establishing a map of the Drosophila larval brain and nerve cord using light microscopy.

Publications

Publications (17)
Article
Full-text available
One challenge is to control epilepsies that do not respond to currently available medications. Since seizures consist of coordinated and high frequency neural activity, our goal was to disrupt neurotransmission with a synaptic transmission mutant and evaluate its ability to suppress seizures. We find that the mutant shibire, encoding Dynamin, suppr...
Article
Full-text available
During development, cell fate decisions are often highly stochastic, but with the frequency of the different possible fates tightly controlled. To understand how signaling networks control the cell fate frequency of such random decisions, we studied the stochastic decision of the Caenorhabditis elegans P3.p cell to either fuse to the hypodermis or...
Article
Full-text available
Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey proteins are respectively fused to an intein N-terminal fragment (IN) and C-terminal fragment (IC) derived from...
Article
Full-text available
Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer important characteristics to tubular organs and need to be carefully controlled. Here, we identify the small coiled-co...
Article
Full-text available
Interactions among proteins are fundamental for life and determining whether two particular proteins physically interact can be essential for fully understanding a protein’s function. We present C. elegans light-induced co-clustering (CeLINC), an optical binary protein-protein interaction assay to determine whether two proteins interact in vivo. Ba...
Article
Full-text available
Crumbs proteins are evolutionarily conserved transmembrane proteins with essential roles in promoting formation of the apical domain in epithelial cells. The short intracellular tail of Crumbs proteins are known to interact with several proteins, including scaffolding protein PALS1 (protein associated with LIN7, Stardust in Drosophila). PALS1 in tu...
Article
Full-text available
Reorganization of the plasma membrane and underlying actin cytoskeleton into specialized domains is essential for the functioning of most polarized cells in animals. Proteins of the ezrin-radixin-moesin (ERM) and Na ⁺ /H ⁺ exchanger 3 regulating factor (NHERF) family are conserved regulators of cortical specialization. ERM proteins function as memb...
Preprint
Full-text available
Reorganization of the plasma membrane and underlying actin cytoskeleton into specialized domains is essential for the functioning of most polarized cells in animals. Proteins of the ezrin-radixin-moesin (ERM) and Na+/H+ exchanger 3 regulating factor (NHERF) family are conserved regulators of cortical specialization. ERM proteins function as membran...
Preprint
Full-text available
The apical domain of epithelial cells can acquire a diverse array of morphologies and functions, which is critical for the function of epithelial tissues. The Crumbs proteins are evolutionary conserved transmembrane proteins with essential roles in promoting apical domain formation in epithelial cells. The short intracellular tail of Crumbs protein...
Preprint
Full-text available
Interactions among proteins are fundamental for life and determining whether two particular proteins physically interact can be essential for fully understanding a protein’s function. We present C. elegans light-induced co-clustering (CeLINC), an optical binary protein-protein interaction assay to determine whether two proteins interact in vivo . B...
Preprint
Full-text available
Epithelial tubes are essential components of metazoan organ systems that control the flow of fluids and the exchange of materials between body compartments and the outside environment. The size and shape of the central lumen confer important characteristics to tubular organs and need to be carefully controlled. Here, we identify the small coiled-co...
Preprint
Cells in developing organisms must robustly assume the correct fate in order to fulfill their specific function. At the same time, cells are strongly affected by molecular fluctuations, i.e. 'noise', leading to inherent variability in individual cells. During development, some cells are thought to exploit such molecular noise to drive stochastic ce...
Article
Full-text available
Invertebrate and vertebrate nervous systems generate different types of dopaminergic neurons in distinct parts of the brain. We have taken a genetic approach to understand how the four functionally related, but lineally unrelated, classes of dopaminergic neurons of the nematode Caenorhabditis elegans, located in distinct parts of its nervous system...
Article
This paper reviews Drosophila voltage-gated Na(+) channel mutations encoded by the para (paralytic) gene and their contributions to seizure disorders in the fly. Numerous mutations cause seizure-sensitivity, for example, para(bss1), with phenotypes that resemble human intractable epilepsy in some aspects. Seizure phenotypes are also seen with human...
Article
Full-text available
We report the larval CNS expression patterns for 6,650 GAL4 lines based on cis-regulatory regions (CRMs) from the Drosophila genome. Adult CNS expression patterns were previously reported for this collection, thereby providing a unique resource for determining the origins of adult cells. An illustrative example re-veals the origin of the astrocyte-...
Article
Genetic factors that influence seizure susceptibility can act transiently during the development of neural circuits, or may be necessary for the proper functioning of existing circuits. We provide evidence that the Drosophila seizure-sensitive mutant easily shocked, (eas), represents a neurological disorder in which abnormal functioning of existing...
Article
Full-text available
Here, we describe the embryonic central nervous system expression of 5,000 GAL4 lines made using molecularly defined cis-regulatory DNA inserted into a single attP genomic location. We document and annotate the patterns in early embryos when neurogenesis is at its peak, and in older embryos where there is maximal neuronal diversity and the first ne...

Network

Cited By

Projects

Projects (3)
Project
Various approaches to understand cell polarity. Development of a novel protein-protein interaction (PPI) detection system.
Archived project
During development, cells must integrate signals and cues from their environment to obtain their proper cell fate. While most cells follow a highly regulated process leading to a single predetermined fate, there are examples of cellular decision making in which a cell may adopt one of many potential fates in a stochastic manner. To understand a stochastic cell fate decision, we will use a recently developed time-lapse microscope to quantify the gene expression and protein dynamics of components of the genetic pathway during the cell fate decision. In addition, we will perform single molecule FISH to quantify mRNA levels in these genes. Overall, the quantitative analysis of gene activity and expression will provide an understanding of how cells receive complex information and then transform that information into a binary outcome.