Jarosław TyburskiNicolaus Copernicus University | umk · Chair of Plant Physiology and Biotechnology
Jarosław Tyburski
PhD
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45
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Publications
Publications (45)
![Fig 2. Methylated DNA fraction [%] of CpG islands in promoter region or...](publication/351927670/figure/fig2/AS:1028350311165954@1622189228011/Methylated-DNA-fraction-of-CpG-islands-in-promoter-region-or-first-exon-of-the-genes_Q320.jpg)
Here we determined the impact of salt shock and salt stress on the level of DNA methylation in selected CpG islands localized in promoters or first exons of sixteen salt-responsive genes in beets. Two subspecies differing in salt tolerance were subjected for analysis, a moderately salt-tolerant sugar beet Beta vulgaris ssp . vulgaris cv. Huzar and...
The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas....
Increasing land salinization in recent decades has led to a decrease in crop productivity worldwide. We hypothesized that bioaugmentation of beetroot (Beta vulgaris) with halotolerant endophytic bacterial strains isolated from the obligatory halophytic plant Salicornia europaea L. may mitigate salt stress in new host plants. Therefore, we investiga...
Microbially assisted phytoremediation is considered as the most promising eco-friendly solution for recultivation of heavy metal polluted soils. In vitro experiments can be favorable systems that allow assessing compatibility and efficiency of both partners (e.g. plant-microorganism) which reduces time and space in the initial stages of this techno...
Background
Salicornia europaea, a succulent obligatory halophyte is the most salt-tolerant plant species in the world. It survives salt concentrations of more than 1 M. Therefore, it is a suitable model plant to identify genes involved in salt tolerance mechanisms that can be used for the improvement of crops. The changes in a plant’s gene expressi...
Background:
Sugar beet is a highly salt-tolerant crop. However, its ability to withstand high salinity is reduced compared to sea beet, a wild ancestor of all beet crops. The aim of this study was to investigate transcriptional patterns associated with physiological, cytological and biochemical mechanisms involved in salt response in these closely...
Recently it was reported that potato pulp, a side product of starch industry, is potentially applicable for the decontamination of phenol-polluted wastewater, due to its high peroxidase content. Regarding the toxicity and the persistence in the environment, the phenol is surpassed by its chlorinated derivatives, such as 2,4-dichlorophenol (2,4-DCP)...
This study found that extracts containing ionically bound proteins, isolated from potato pulp and brewers' spent grain, were characterized by high peroxidase activities. The kinetic parameters, namely Km and Vmax values, were typical for plant peroxidases. Seven peroxidase isoenzymes in potato pulp and two isoenzymes in brewers' spent grain were ob...
Bioaugmentation of soils with selected microorganisms during phytoextraction can be the key solution, but the actual efficacy of this technology should be confirmed for different physicochemical soil parameters and heavy metal availability to guarantee the universality of this method. Equally important is development of the accurate prediction tool...
The efficiency of phytoremediation might be highly affected by plant-associated microorganisms, and understanding of the underlying mechanisms is still a great challenge. The primary aim of this study was to evaluate the efficiency parameters for Cd2+ accumulation in the biomass of willow (Salix viminalis) as well as to define the biochemical respo...
Beta vulgaris ssp. maritima is a halophytic relative of cultivated beets. In the present work a transcriptome response to acute salt stress imposed to excised leaves of sea beet was investigated. Salt treatments consisted of adding NaCl directly to the transpiration stream by immersing the petioles of excised leaves into the salt solutions. Sequenc...
Plant peroxidases have strong potential utility for decontamination of phenol-polluted wastewater. However, large-scale use of these enzymes for phenol depollution requires a source of cheap, abundant, and easily accessible peroxidase-containing material. In this study, we show that potato pulp, a waste product of the starch industry, contains larg...
Salt tolerance is largely dependent on a plant’s ability to maintain optimal water status in leaves. The adjustment of water relations under salinity involves changes in the transcriptional activity of genes encoding plasma membrane aquaporins (PIPs). Here, we report the effects of long-term or short-term treatments with moderate or strong salt str...
BvpAPX is a full-length cDNA-encoding peroxisomal ascorbate peroxidase isolated from leaves of salt-stressed beet (Beta vulgaris) plants. A high level of identity has been reported between the deduced amino acid sequence of BvpAPX and other known ascorbate peroxidases. The genomic sequence of BvpAPX revealed a gene composed of 5 exons and 4 introns...
Differentially expressed transcript derived fragments (TDFs) of bovine pituitary gland tissue at different developmental ages of Limousine and Hereford bulls were identified by cDNA-AFLP technique. Study revealed comparatively higher differentially expressed transcripts in 6-month Limousine bulls and 12-month Hereford bulls. The BLASTn/p analysis i...
##Assembly-Data-START## Assembly Method :: CLC Sequence Viewer v. 5.1.1 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
The study utilized age-dependent breed-specific gene expression profiling by cDNA-AFLP technique in identification of differentially expressed (DE) candidate genes for growth and development trait. Results revealed 15579 transcript derived fragments (TDFs) expressed in bovine pituitary gland tissue using 96 unique PCR reactions with primer combinat...
Arabidopsis root system responds to phosphorus (P) deficiency by decreasing primary root elongation and developing abundant lateral roots. Feeding plants with ascorbic acid (ASC) stimulated primary root elongation in seedlings grown under limiting P concentration. However, at high P, ASC inhibited root growth. Seedlings of ascorbate-deficient mutan...
The prerequisite for shoot, root or somatic embryo formation in plant in vitro culture is the development of meristem from
dedifferentiated cells of the explant tissue. Auxin and cytokinin levels and their relative ratios play a decisive role in
inducing the morphogenetic pathways leading to shoot, root or somatic embryo formation in plant in vitro...
To study the relationship between glutathione and rooting, tomato seedling cuttings, grown on basal- or on auxin-supplemented
media, were treated with the reduced (GSH) or oxidized (GSSG) form of this antioxidant. In turn, the consequences of the depletion
of GSH pool on rooting were tested using l-buthionine sulfoximine (BSO), a specific inhibitor...
ABSTRACT
Ascorbic acid (ASC), a small molecule antioxidant, is commonly found in plant tissues. Antioxidative functions of that compound are implemented in Halliwell – Asada cycle, which comprises a group of biochemical processes, providing detoxification of H2O2 and regeneration of the reduced form of ascorbate. Apart from its pivotal role in oxi...
The developmental response of the Arabidopsis root system to low phosphorus (P) availability involves the reduction in primary root elongation accompanied by the formation of numerous lateral roots. We studied the roles of selected redox metabolites, namely, radical oxygen species (ROS) and ascorbic acid (ASC) in the regulation of root system archi...
The developmental response of the Arabidopsis root system to low phosphorus (P) availability involves the reduction in primary root elongation accompanied by the formation of numerous lateral roots. We studied the roles of selected redox metabolites, namely, radical oxygen species (ROS) and ascorbic acid (ASC) in the regulation of root system archi...
The aim of this study was to test the effect of auxin treatment on selected parameters of the redox metabolism in roots. We
found that auxin application results in a reduction in the H2O2 level in roots. The hormone stimulated CuZn-superoxide dismutase, but simultaneously increased the activities of catalase,
cell wall bound ferulic acid peroxidase...
Ascorbate levels and redox states, as well
as the activities of the enzymes of ascorbate metabolism,
were analyzed in roots of tomato seedlings during
the culture on a medium supplemented with auxin and
compared to the control cultured on an auxin-free
medium. Biochemical parameters were determined
separately in the distal part of the root where th...
Arabidopsis plants responding to phosphorus (P) deficiency increase lateral root formation and reduce primary root elongation. In addition
the number and length of root hairs increases in response to P deficiency. Here we studied the patterns of radical oxygen
species (ROS) in the roots of Arabidopsis seedlings cultured on media supplemented with h...
Ascorbate levels and redox states, as well as the activities of the enzymes of ascorbate metabolism, were analyzed in roots of tomato seedlings during the culture on a medium supplemented with auxin and compared to the control cultured on an auxin-free medium. Biochemical parameters were determined separately in the distal part of the root where th...
Real-time PCR has become one of the most widely used methods of gene quantitation in molecular biology and medical diagnostics. This technique combines PCR amplification and the detection of the PCR product into a single step. In real-time PCR, the amount of product formed is measured during the course of the experiment by monitoring the fluorescen...
The accuracy of real-time PCR (RT-PCR) experiment is strongly dependent on the mathematical models of data analysis, on which the quantitative methods are based. In this review, we discuss the key steps of analysing data from real-time PCR experiments. These are the treshold cycle determination, estimation of real-time PCR amplification efficiency...
The changes in ascorbate (ASC) and dehydroascorbate (DHA) levels and the activities of ascorbate metabolising enzymes were
examined during adventitious root formation in cuttings of tomato (Lycopersicon esculentum Mill. cv. Paw) seedlings. The effects of ASC, DHA and the immediate ascorbate precursor – galactono-γ-lactone (GalL) supplemented to the...
A relationship between light conditions, auxin transport and adventitious root formation by hypocotyls of tomato seedling cuttings was demonstrated. Effective rooting of tomato seedling cuttings was observed under continuous white light (WL) irradiation. However, root formation was reduced in darkness or under red (RL) or blue light (BL). At least...
Conventional methods used in bacteriological examinations of foodstuffs are time-consuming and do not always result in the correct identification of the microorganism. For this reason laboratories more and more frequently employ highly sensitive, molecular techniques. One of them is the microarray DNA method. The aim of the study was to check the u...
A relationship between light conditions, auxin transport and adventitious root formation by hypocotyls of tomato seedling cuttings was demonstrated. Effective rooting of tomato seedling cuttings was observed under continuous white light (WL) irradiation. However, root formation was reduced in darkness or under red (RL) or blue light (BL). At least...
Metodą DD (ang. differential display) PCR porównano populację RNA pochodzącego z liścieni, kalusa otrzymanego z liścieni oraz kalusa otrzymanego z eksplantatów hypokotyli roślin Pharbitis nil. Obraz elektroforetyczny badanych RNA jest różny, co wskazuje na zmianę wzorca ekspresji genów wielokopiowych, w tym rRNA w tkance kalusowej.
Stosując technik...
The effect of white (WL) and red (RL) light on organogenesis in vitro was studied using explants isolated from seedlings of wild-type (WT) and two photomorphogenic mutants of tomato (Lycopersicon esculentum Mill.) - aurea (au) and high pigment (hp). Explants excised from hypocotyls and cotyledons of green and etiolated seedlings were cultured on sh...
Projects
Projects (3)
The main research hypothesis of the project is that endophytic microbiome of halotolerant plant may contribute to drought tolerance. Further we think that the microbiome may function and exert its effect on non-native plants as well, which might be termed 'compatibility'. The aim of planned investigations is to evaluate the contribution of endophytic microorganisms to drought stress tolerance in sugar beet.
Determining kinetic properties of peroxidases from new sources, fractionation of crude extracts obtained from potato pulp and brewers' spent grain.
