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Janaína dos Santos Nascimento

Janaína dos Santos Nascimento
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro · Microbiology

PhD

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73
Publications
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Publications

Publications (73)
Article
Full-text available
Abstract Listeria monocytogenes is a gram-positive bacterium that can survive in food production environments and food products. This microorganism is associated with listeriosis, a serious infection caused by the consumption of contaminated food. The aim of this study was to conduct an integrative literature review on the regulation of L. monocyto...
Article
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Abstract It is estimated that infections caused by bacteria of to the genus Vibrio are sub notified, however it is possible to notice the increase in the vibriosis incidence associated to the consumption of contaminated water and food, specially by raw seafood. Vibrio cholerae, Vibrio parahaemolyticus e Vibrio vulnificus are the main pathogenic spe...
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Abstract The psychrotrophic bacteria count and the profile of Gram-negative bacteria present in commercial Brazilian organic dairy products (27 samples, pasteurized whole milk, Minas Frescal cheese, and yoghurt, equally distributed) as well as the biofilm-producing capacity and the production of deteriorating enzymes were investigated. Most of the...
Article
Introduction: Enterobacteriaceae are often reported as a typical bacterial population in raw milk from any mammalian origin. The frequent concern with bacteria, especially those related to this group of microorganisms, is their increasing resistance to antibiotics and the emergence of enzymes that degrade them. This study aimed to characterize iso...
Article
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Conventional culture media are expensive owing to their constituents. Thus, several studies have sought to develop and evaluate the efficacy of alternative, low-cost culture media, in most cases, using natural and easily accessible raw materials. The present study is a literature review, observing various formulations of culture media based on prod...
Article
Background and Objective: Bacteria of the Genus Kocuria are found in several environments and their isolation from foods has recently increased due to more precise identification protocols using molecular and instrumental techniques. This review describes biotechnological properties and food-linked aspects of these bacteria, which are closely a...
Article
Background Quantitative microbiological risk assessment (QMRA) is the most complex stage of risk analysis, an important management tool that considers the entire food production chain. Through safety limits, health authorities and producers are concerned with achieving the minimum risk to consumer health. Dairy products are a class of foods very su...
Article
The effect of ohmic heating (OH) (50, 55, and 60 °C, 6 V cm⁻¹) on the inactivation kinetics (Weibull model) and morphological changes (scanning electron microscopy and flow cytometry) of Salmonella spp. in infant formula (IF) was evaluated. In addition, thermal load indicators (hydroxymethylfurfural and whey protein nitrogen index, HMF, and WPNI) a...
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Macrococcus spp. are Gram-positive cocci that belong to the Staphylococcaceae family; they are closely related to staphylococci, but, unlike staphylococci, they are not considered as human pathogens. Macrococcus spp. are recognized as relevant veterinary pathogens, and their presence has been reported in food products of animal origin. Macrococcus...
Article
Most of the culture media used in bacterial growth is composed of complex ingredients, increasing the value of the product. This makes its acquisition unavailable by educational institutions without sufficient funding, making even more difficult the practical teaching of microbiology. Therefore, the development of an alternative medium of simple co...
Article
Some species of the genus Acinetobacter are admittedly important hospital pathogens. Additionally, various animal and plant foods have been linked to the presence of Acinetobacter, including resistant strains. However, due to isolation difficulties and the lack of official standard methods, there is a dearth of work and epidemiological data on food...
Article
Background: Milk is a reservoir for several groups of microorganisms, which may pose health risks. The aim of this work was to assess the antibiotic resistance and biofilm production in catalase-positive Gram-positive cocci isolated from Brazilian pasteurized milk. Methods: The bacteria were isolated using Baird-Parker agar and identified by Matrix...
Article
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Although quite controversial from the point of view of food safety, a considerable number of people across different age and socioeconomic groups consume raw goat’s milk. Even if refrigerated after milking, raw goat's milk may still be susceptible to the lipolytic and proteolytic actions of psychrotrophic microorganisms, such as Pseudomonas. In thi...
Article
RESUMO O consumo e a produção de leite caprino vêm aumentando nos últimos anos. Um dos motivos é o seu menor potencial alergênico, comparado ao leite bovino. Porém, em ambos os casos, a cadeia produtiva apresenta fatores de risco, sendo necessário tratamento o térmico antes do consumo. Neste trabalho, foram avaliadas 21 amostras de leite caprino cr...
Article
The inactivation kinetics of Listeria monocytogenes in whey dairy beverage submitted to ohmic heating processing (OH, 3 V/cm, 57.5–60 °C for 38-4 min) was investigated. Besides, the physicochemical characterization and sensory analysis of the whey dairy beverage processed by OH were performed. OH at 65 °C resulted in a reduction rate (RR) of 2.10 l...
Article
Introdução: Bactérias do grupo dos coliformes têm sido empregadas ao longo dos anos como micro-organismos indicadores da qualidade microbiológica. No entanto, diversos estudos relatam a falta de correlação direta entre sua presença e a de patógenos na avaliação da segurança de alimentos, levando a sugestão de novas propostas de análise, como a pesq...
Article
This study aimed to study the behavior of Salmonella submitted to domestic microwave through the use of predictive microbiology. The results showed reductions of 9.22, 9.59, 8.23, and 8.57 log CFU/mL in Salmonella counts after exposure to microwave heating at 20 W (750 s), 40 W (90 s), 60 W (120 s), and 80 W (120 s), respectively, with a maximum te...
Article
Reducing salt content in foods such as cheeses, while limiting the growth of spoilage microorganisms and foodborne pathogens, is a difficult challenge. One method that may prove useful is use of staphylococcins, which are bacteriocins produced by staphylococci. Therefore, staphylococcin antimicrobial activity against six strains of S. aureus isolat...
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Goat’s milk has been suggested as an alternative to cow’s milk, being a better digestible and hypoallergenic option. However, the presence of contaminating bacteria may significantly affect the safety of the product. In this research, we reported the isolation and characterization of Acinetobacter spp. isolates from raw goat milk samples purchased...
Article
Introduction: Antimicrobial substances (AMS) produced by bacteria may reduce or prevent the growth of pathogenic and spoilage microorganisms in food. In this study, 16 isolates of Acinetobacter baumannii/calcoaceticus (ABC) complex, previously obtained from reconstituted infant milk formula (IMF) samples and the preparation and distribution utensi...
Article
The aim of this study was to determine the occurrence, counts, and diversity of spore-forming bacteria in three types (full fat, low fat, and flavored full fat) of “requeijão cremoso”. In addition, the growth potential (δ) of three prevalent species of spore-forming bacteria was determined in samples stored at three different temperatures. The occu...
Article
In September 2005, the Sanitary Surveillance Service of Rio de Janeiro (SSS/RJ), Brazil, investigated a case of gastroenteritis involving a 13-year-old teenager hospitalized because of bloody diarrhea and severe abdominal pain. Owing to the severity of the symptoms, an epidemiological investigation was conducted in two states of Brazil. Escherichia...
Article
In this study, the thermal inactivation kinetics of Paenibacillus sanguinis 2301083PRC and Clostridium sporogenes JCM1416MGA were determined in full and low fat “requeijão cremoso”. Requeijão cremoso” is a type of processed cheese widely produced and consumed in Brazil. From a total of 79 strains of six different species of sporeforming bacteria is...
Article
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Dairy products are the base of the diet of many families in all social classes worldwide. However, the high content of milk nutrients and their derivatives, in addition to the near neutral pH and high water activity, provide an ideal environment for the growth of many microorganisms in dairy products (Oladipo and Omo-Adua, 2011). Moreover, during i...
Article
The effect of partial substitution of NaCl with KCl and the flavor enhancers addition (arginine, yeast extract and oregano extract) on Probiotic Prato cheese processing with (L. casei 01, 7 log CFU/mL) was investigated. Microbiological (lactic acid bacteria and probiotic counts), physicochemical (proximate composition, pH, proteolysis), bioactivity...
Article
Sheep milk has a high nutritional value and high concentrations of proteins, fats, minerals, and vitamins, as compared to the milks of other domestic species. The physicochemical and nutritional characteristics of sheep milk can be advantageous for the manufacture of products containing prebiotic ingredients and/or probiotic bacteria, which are maj...
Article
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The increasing prevalence of foodborne diseases observed in developing countries has been linked to a rise in the consumption of raw foods. However, unlike the classical pathogens that are commonly implicated in foodborne illnesses, members of the genus Acinetobacter are rarely associated with diarrheal disease, probably because of the difficulty i...
Article
Background Processed cheese is produced by blending natural cheese of different ages and degrees of maturity in the presence of emulsifying salts and other dairy and nondairy ingredients, followed by heating and continuous mixing to form a homogeneous product with an extended shelf life. However, due to the thermal processing applied and the extend...
Article
Infant milk formulas are not sterile products, and pathogenic bacteria can survive and multiply in these products. This study was performed, initially, to detect the presence of Salmonella spp. in reconstituted infant milk formula and on utensils previously sanitized used in their preparation or distribution in a nursery of a public hospital in Rio...
Article
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In this study, 15 Gram-negative isolates from Minas Frescal cheese sold in commercial establishments in Rio de Janeiro, Brazil, were able to produce antimicrobial substances (AMSs). Seven, four, two, one, and one isolates identified as Yersinia, Acinetobacter, Enterobacter, Escherichia, and Hafnia genera, respectively, were considered potentially p...
Article
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Salmonella enterica é um dos mais importantes patógenos associados a fórmulas lácteas infantis (FLI). Neste trabalho, foi avaliada a sobrevivência e crescimento de uma estirpe padrão de Salmonella enterica sorotipo Typhi em duas FLI reconstituídas (F1 e F2) sob diferentes condições de preparo e armazenamento similares às utilizadas em lactários hos...
Article
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O Programa de Aquisição de Alimentos (PAA) possui uma modalidade na qual os agricultores familiares vendem sua produção para o Governo e entregam diretamente em rede socioassistencial, atendendo populações em situação de insegurança alimentar e nutricional. No ano de 2012, o município de Cardoso Moreira participou do PAA e, nesta ocasião, foi const...
Article
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Neste trabalho, a produção de substâncias antimicrobianas por estirpes de Pseudomonas sp. isoladas de leite pasteurizado e seu potencial de ação contra bactérias associadas a alimentos foram investigados. As amostras de leite pasteurizado foram adquiridas em estabelecimentos comerciais, arbitrariamente escolhidos, da cidade do Rio de Janeiro-RJ, Br...
Article
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The standard most used in testing laboratories, ISO/ IEC 17025:2005, describes the participation of laboratories in periodic proficiency testing (PT), as a criteria for quality assurance of analytical results. The analyses used in PT are reference materials (MR) from the same lot, and must have characteristics of homogeneity and stability. This stu...
Article
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The use of reference materials (RM) is one of the principal tools used for assurance and quality control in food microbiology laboratories. In Brazil, Anvisa RDC nº 12/01 specifies the enumeration of coagulase-positive staphylococcus (CPS) as one of the parameters for evaluating cheese quality. The main challenge in the production of RM destined fo...
Article
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  The main aim of this study was to analyse the genetic relationship amongst 46 Staphylococcus aureus Bac(+) strains isolated in Brazil from 12 geographically distant dairy herds, including 34 isolates that produce the antimicrobial peptide aureocin A70.   The comparison of 46 Staph. aureus Bac(+) strains was performed by pulsed-field gel electroph...
Article
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Aureocin A53 is an antimicrobial peptide produced by Staphylococcus aureus A53. The genetic determinants involved in aureocin A53 production and immunity to its action are organized in at least four transcriptional units encoded by the 10.4-kb plasmid pRJ9. One transcriptional unit carries only the bacteriocin structural gene, aucA. No immunity gen...
Article
O objetivo deste trabalho consistiu na investigacao da producao de substâncias antimicrobianas por estirpes de Bacillus, isoladas de frutas, e verificar o potencial dessas substâncias na inibicao de patogenos, associados a deterioracao ou a transmissao de doencas por alimentos, como fungos e bacterias Gram-negativas ou Gram-positivas. Dez estirpes...
Article
The present work aimed to investigate the production of a new antimicrobial substances by Bacillus sp. isolated from fruit, and verify its potential as a pathogen inhibitor associated to deterioration and food disease transmission, such as fungus and Gram-positive and Gram-negative bacteria. Ten strains were submitted to Gram stain and showed to be...
Article
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Nukacin 3299 (formerly designated simulancin 3299), produced by a Staphylococcus simulans strain involved in bovine mastitis in Brazil, is the first peptide bacteriocin described in this staphylococcal species. With the intent to elucidate some aspects of its biology, nukacin 3299 was purified and characterized. The mass of the purified bacteriocin...
Article
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S.S. DE OLIVEIRA, J. DOS SANTOS NASCIMENTO, D.C. PÓVOA, S.A. DE ARAÚJO, M.R. GAMON AND M C DE FREIRE BASTOS. 1998. pRJ6 and pRJ9, small Staphylococcus aureus plasmids which code for bacteriocins, exhibited a bactericidal activity against several lactic acid bacteria and strains of Listeria monocytogenes, an important food-borne pathogen. Filter-mat...
Article
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In the present study, the bacteriocins produced by Staphylococcus aureus 4185, a strain isolated from bovine mastitis, were purified and partially characterized. After purification by ammonium sulfate precipitation, cation-exchange chromatography, and five runs of high-performance liquid chromatography (HPLC), antimicrobial activity was recovered w...
Article
In the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in Rio de Janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. Thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to n...
Article
Full-text available
In the present study, 257 Staphylococcus spp. strains were isolated from bovine mastitis cases in 56 different Brazilian dairy herds located in the southeast region of the country and tested for antimicrobial substance (AMS) production. Forty-six strains (17.9%) exhibited AMS production and their identification as Staphylococcus aureus was based on...
Article
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Bacteriocins are bacterial antimicrobial peptides with bactericidal activity against other bacteria. Staphylococcins are bacteriocins produced by staphylococci, which are Gram-positive bacteria with medical and veterinary importance. Most bacteriocins produced by staphylococci are either lantibiotics (e.g., Pep5, epidermin, epilancin K7, epicidin 2...
Article
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A salada é um dos alimentos mais consumidos em restaurantes “self service”, entretanto, pode atuar como um importante veículo de transmissão de bactérias do grupo dos coliformes. Neste trabalho, foi realizada a quantificação de coliformes totais e termotolerantes presentes em saladas cruas e cozidas comercializadas em restaurantes do tipo “self-ser...
Article
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The inhibitory activity of seven bacteriocins produced by Staphylococcus aureus (aureocins A70, A53, and 215FN) and Staphylococcus epidermidis (Pep5, epidermin, epilancin K7 and epicidin 280) was tested against strains of both S. aureus (165 strains) and Streptococcus agalactiae (74 strains) isolated from udders of cows suffering from bovine mastit...
Article
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To investigate the activity of seven staphylococcins, bacteriocins produced by staphylococci, against multiresistant Staphylococcus aureus and coagulase-negative staphylococci (CNS) involved in human infections. Four bacteriocins produced by Staph. epidermidis (Pep5, epidermin, epilancin K7 and epicidin 280) and three produced by Staph. aureus (aur...
Article
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Among 363 strains of Staphylococcus aureus, 21 were shown to produce bacteriocins (Bac), antimicrobial peptides with potential biotechnological applications. This collection includes strains which are either isolated from food, patients and healthy cattle, or are involved in subclinical bovine mastitis. From these 21 strains, 17 were shown to carry...
Article
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In the present study, 188 coagulase-negative Staphylococcus (CNS) strains were isolated from bovine mastitis cases from 56 different Brazilian dairy herds, located in the Southeast region of the country, and were tested for antimicrobial substance production. Twelve CNS strains (6.4%) exhibited antagonistic activity against a Corynebacterium fimi i...
Article
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The influence of growth parameters on the production of bacteriocins (aureocins) by five strains of Staphylococcus aureus was investigated. These aureocins have a broad spectrum of activity and can inhibit important human and animal pathogens. All strains produced large inhibition zones upon the indicator strain when they were grown in rich media s...
Article
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Fifty strains of Staphylococcus spp. isolated from bovine mastitis cases in several herds from different Argentinian provinces were screened for antimicrobial substances. Twelve strains exhibited a high antagonistic activity against the indicator strain (Corynebacterium fimi) and were chosen for further characterisation. The antimicrobial substance...
Article
Fifty strains of Staphylococcus spp. isolated from bovine mastitis cases in several herds from different Argentinian provinces were screened for antimicrobial substances. Twelve strains exhibited a high antagonistic activity against the indicator strain (Corynebacterium fimi) and were chosen for further characterisation. The antimicrobial substance...
Article
Full-text available
Staphylococcus aureus A70 produces a heat-stable bacteriocin designated aureocin A70. Aureocin A70 is encoded within a mobilisable 8 kb plasmid, pRJ6, and is active against Listeria monocytogenes. Experiments of transposition mutagenesis and gene cloning had shown that aureocin A70 production and immunity were associated with the HindIII-A and B fr...
Article
Full-text available
pRJ6 and pRJ9, small Staphylococcus aureus plasmids which code for bacteriocins, exhibited a bactericidal activity against several lactic acid bacteria and strains of Listeria monocytogenes, an important food-borne pathogen. Filter-mating experiments using plasmid derivatives tagged with either Tn551 or Tn917-lac showed that pRJ6, but not pRJ9, cou...
Article
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Among 46 isolates of Staphylococcus aureus obtained from cattle in the State of Paraíba, Brazil, four were shown to produce antimicrobial substances (AMS). The two best AMS producers carried single plasmids of about 8.0 kbp and 50 kbp, respectively, which were designated pRJ34 and pRJ35. Curing experiments and molecular analysis associated the AMS...