Janaína Dernowsek

• PhD
• Researcher and CEO at BioEdTech & QUANTIS

We established a proof-of-concept model system for the biological healing of periapical lesions using stem cell spheroids. Mesenchymal stem cells from human exfoliated deciduous teeth (SHED) were cultured in a 2D monolayer and then as 3D multicellular spheroids. An image of a periapical lesion of an upper lateral incisor tooth was obtained by compu...

Advances in 3D cell culture have developed more physiologically relevant in vitro models, such as tissue spheroids. Cells cultivated as spheroids have more realistic biological responses that resemble the in vivo environment. Due to their advantages, tissue spheroids represent an emerging trend toward superior, more reliable, and more predictive st...

Several techniques, such as additive manufacturing, have been used for the manufacture of polymer-ceramic composite scaffolds for bone tissue engineering. A new extruder head recently developed for improving the manufacturing process is an experimental 3D printer Fab@CTI that enables the use of ceramic powders in the processing of composite materia...

Background Besides controlling the expression of peripheral tissue antigens, the autoimmune regulator (AIRE) gene also regulates the expression of adhesion genes in medullary thymic epithelial cells (mTECs), an essential process for mTEC-thymocyte interaction for triggering the negative selection in the thymus. For these processes to occur, it is n...

This chapter provides a review of recent advances in understanding the importance of normal cell differentiation and the key regulators that orchestrate their fate into several cell types. The cell source is an essential factor that must be considered for achieving large quantities, having a fast proliferation rate, a significant expansion capabili...

The cultivation of cells in 3D has gained more interest in research once 3D architecture can be closer to full cell physiological functionality. The cultivation of the cells in a spheroid format has shown very promising results, further for bioprinting developing so fast during the last decade. The interaction of spheroids and the matrix, or bioink...

The aim of this study was to investigate the printability of different compositions of biomaterials to be potentially used in bioprinting of cartilaginous substitutes. Part of the compositions contained pulverized devitalized cartilage (DVC), which confers the necessary biochemical complexity for bioprinted scaffolds, and some compositions containe...

Besides controlling the expression of peripheral tissue antigens, the autoimmune regulator (AIRE) gene also regulates the expression of adhesion genes in medullary thymic epithelial cells (mTECs), an essential process for mTEC-thymocyte interaction for triggering the negative selection in the thymus. For these processes to occur, it is necessary th...

We established a proof of concept model system for the biological repair of periapical lesions using stem cell spheroids. A mesenchymal stem cell line isolated from the dental pulp of deciduous teeth (shed cells) was cultured in a 2D monolayer and then in 3D multicellular spheroids. An image of a periapical lesion of an upper lateral incisor tooth...

Recentes avanços na manufatura aditiva têm permitido a impressão 3D de materiais biocompatíveis, células e componentes estruturais. Um desses principais componentes é o colágeno tipo I que se tornou um grande propulsor das indústrias farmacêutica, médica, alimentícia e cosmética. Considerando as novas tendências em bioengenharia e métodos alternati...

Background Nerve injuries are debilitating, leading to long-term motor deficits. Remyelination and axonal growth are supported and enhanced by growth factor and cytokines. Combination of nerve guidance conduits (NGCs) with adipose-tissue-derived multipotent mesenchymal stromal cells (AdMSCs) has been performing promising strategy for nerve regenera...

Background: Nerve injuries are debilitating, leading to long-term motor deficits. Remyelination and axonal growth are supported and enhanced by growth factor and cytokines. Combination of nerve guidance conduits (NGCs) with adipose-tissue-derived multipotent mesenchymal stromal cells (AdMSCs) has been performing promising strategy for nerve regener...

O presente objeto possui aplicação na área de biotecnologia e saúde, mais especificamente nos segmentos farmacêuticos, cosméticos, estéticos e engenharia tecidual. O método para a produção de uma composição de proteínas de matriz extracelular.

Piezoelectric inkjet 3D bioprinting technology is a viable technique for ophthalmological applications. It provides versatility, high sensibility and accuracy, required in ophthalmological procedures. A process flow for biofabrication was described in detail and validated, using piezoelectric inkjet technology, for ophthalmological applications, in...

The demand for organ donations is still considerably higher than the number of donors. The development of techniques for biofabricating tissues can reduce the crisis of organ shortage. The biofabrication of organs is approached by Tissue Engineering and its objective is the assembly of functional tissues and organs. Bioprinting can generate complex...

O propósito da BioEdTech é a capacitação de estudantes, profissionais e empresas em bioimpressão de tecidos de forma prática, desmitificada e dinâmica, focada nos desafios da área, para acelerar o desenvolvimento produtivo da tecnologia e de novos produtos por meio de parcerias construtivas e integrativas.

CURSOS DE CAPACITAÇÃO EM BIOIMPRESSÃO "Construa a sua BioimPressora" BioEdTech e Merck acelerando o avanço da Bioimpressão no Brasil. Vamos apresentar os programas mais utilizados na MODELAGEM 3D e modelagem BIOLÓGICA, como configurar o FIRMWARE, como CALIBRAR e dar a MANUTENÇÃO adequada, como simular alguns processos reológicos de BIOTINTAS e...

The biofabrication of engineered tissues is an essential area to reconstruct tissues. Until now, to the best of our knowledge, it has not been possible to mimic the biological and biochemical properties. New approaches to developing a new tissue become an attractive target for bioprinting, which is emerging as an essential strategy to recreate the...

Recent research evidences substantial morphological and physiological differences between cells maintained in vitro in two‐dimensional (2D) and three‐dimensional (3D) culture conditions. The current consensus is that 3D culture is better because it precisely mimics the cellular microenvironment in vivo . The biomodulatory effect of low‐intensity la...

Hoje com o avanço tecnológico estamos conhecendo um mundo totalmente novo voltado para pesquisas medicinais com a engenharia tecidual, direcionada para formação de tecidos in silico, in vitro ou in vivo. Uma das abordagens da engenharia de tecidos implica a produção de scaffolds, suportes necessário para adesão, proliferação e diferenciação mantend...

Increasingly, biofabrication is seen as a promising strategy in the tissue engineering and regenerative medicine fields. It proves to be a good alternative for drug and cosmetics testing and even for transplantation tissues and organs in humans. However, long before we dream with this science impacting our daily lives, we need to know it more on a...

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A Engenharia Tecidual é uma área interdisciplinar, em que o conhecimento pode ser aplicado na criação, regeneração e aperfeiçoamento de técnicas na medicina para reposição de tecidos. A impressão 3D surge em meio à engenharia tecidual com novas possibilidades, como a impressão a partir de um modelo tridimensional virtual que mimetize uma estrutura...

Material produzido após o meetup do dia 17/01 em São Paulo. Conteúdo do E-book: 1.Introdução Biofabricação Bioimpressão 2.Biotinta 3.Etapas da Bioimpressão 4.Interdisciplinaridade da Bioimpressão 5.Tipos de estimulação celular 6.Desafios 7.Realidade X Futuro 8.Questões importantes https://docs.wixstatic.com/ugd/ec99be_697481f44fb74ed283b1be276823...

Este trabalho tem como finalidade estudar geometrias diferentes de scaffolds tridimensionais (3D), que são estruturas que dão suporte e sustentação para as células como estratégia 3D da engenharia tecidual. As células se desenvolvem quando estão dimensionadas em nichos tridimensionais, demonstrando aderência e funções biológicas próximas às reais,...

Bioprinting is the utilization of techniques derived from three-dimensional printing to generate complex biological structures which may replace natural tissues or organs. It employs high spatial resolution deposition of different cell types, growth factors and biomaterials. Those together form bioinks, which are the bioprinting inputs, analogously...

Increasingly, biofabrication is seen as a promising strategy in the tissue engineering and regenerative medicine fields. It proves to be a good alternative for drug and cosmetics testing and even for transplantation tissues and organs in humans. However, long before we dream with this science impacting our daily lives, we need to know it more on a...

Resumo. A demanda por doações de órgãos ainda é expressivamente maior que o número de doadores e o desenvolvimento de técnicas para biofabricar tecidos podem aliviar a crise de escassez de órgãos. A biofabricação de órgãos é abordada pela Engenharia de Tecidos e tem como objetivo a recuperação e regeneração de tecidos e órgãos. A bioimpressão pode...

A biofabricação como área interdisciplinar está promovendo novos conhecimentos e integração de áreas como nanotecnologia, química, biologia, física, ciência dos materiais, sistemas de controle, bioinformática, engenharias, as quais são fundamentais para realizar o desafio da bioengenharia de tecidos complexos Funcionais. O surgimento de plataformas...

E hoje vamos falar dos grandes desafios presentes na área de bioimpressão tecidual. Por que ainda não temos tecidos funcionais complexos "bioimpressos"? Os desafios presentes na bioimpressão de tecidos e órgãos são inúmeros. Podemos dividir esses desafios em Desafios tecnológicos, biológicos, químicos, físicos, de materiais, éticos e de regulamenta...

Resumo Este trabalho tem como finalidade estudar geometrias diferentes de scaffolds tridimensionais (3D) que são estruturas que dão suporte e sustentação para as células como estratégia 3D da engenharia tecidual. As células se desenvolvem quando estão dimensionadas em nichos tridimensionais, demonstrando aderência e funções biológicas próximas às r...

A Engenharia Tecidual é uma área interdisciplinar, em que o conhecimento pode ser aplicado na criação, regeneração e aperfeiçoamento de técnicas na medicina para reposição de tecidos. A impressão 3D surge em meio à engenharia tecidual com novas possibilidades, como a impressão a partir de um modelo tridimensional virtual que mimetize uma estrutura...

3D Bioprinting of tissues and organs: new technologies aimed at medicine 4.0

BioengenhariaTecidual: Biological Computer Aided Engineering -BIOCAE

BioCAE is a new computational approach to understanding complex biological tissue systems using a combination of methods such as multiscale mathematical modeling, computational simulations, functional biological data mining, simulation of structure, material and cell behavior, regulatory network, machine learning of omic data, integrated with syste...

Bio3Data, startup de tecnologia que desenvolve soluções SaaS para a área biológica com o intuito de minimizar o tempo, os custos e o uso de animais nas pesquisas. Desenvolvemos BIOMODELOS PREDITIVOS de fenômenos biológicos utilizando dados ômicos, inteligência artificial e simulação computacional para a educação, engenharia tecidual, biofabricação...

Increasingly, biofabrication is seen as a promising strategy in the tissue engineering and regenerative medicine field. It proves to be a good alternative for drug and cosmetics testing and even for transplantation tissues and organs in humans. However, long before we dream with this science impacting our daily lives, we need to know it more on a s...

The understanding of the biomechanical and biological phenomena in the bioengineering studies needs for innovation and specific computational methods development. Computer simulations for bioengineering purposes are an alternative method for better understanding and potential reduction of investments of large amount of money and time with in vitro...

The goal is to analyze the impact of stress on the hierarchical layers of the cartilage using finite element method. his work presents an approach that allows predicting the behavior of biological processes such as angiogenesis, molecules diffusion and cells interactions, besides to provide a new outlook in the biofabrication of tissues and organs.

Significant efforts have been made to develop various molds for application in tissue engineering and biofabrication. A range of factors affect the behaviors of cells on a substrate, including surface chemistry, feature geometry, and elastic modulus. The ability to control the position of cells in an organized pattern on a substrate has become incr...

A medicina 4.0, fazendo uma alusão à indústria 4.0, engloba fortemente a biofabricação de tecidos e órgãos, baseando-se numa miríade de emergentes tecnologias, especialmente no campo da medicina regenerativa, tratamento e diagnósticos personalizados e na descoberta de novos fármacos. Assim sendo, o avanço da impressão 3D, bioimpressão, microfluídic...

Este trabalho propõe o desenvolvimento de um modelo virtual preditivo para os processos da biofabricação e bioimpressão, por meio da integração das áreas biológica, exata e computacional. O uso de biomodelos preditivos na bioimpressão aumentarão a compreensão dos sistemas vivos, relacionando o comportamento básico das moléculas a complexas interaçõ...

The biofabrication of engineered tissues is an essential area to reconstruct tissues. Until now, to the best of our knowledge, it has not been possible to mimic the biological and biochemical properties. New approaches to developing a new tissue become an attractive target for bioprinting, which is emerging as an essential strategy to recreate the...

Increasingly, biofabrication is seen as a promising strategy in the tissue engineering and regenerative medicine field. It proves to be a good alternative for drug and cosmetics testing and even for transplantation tissues and organs in humans. However, long before we dream with, science impacting our daily lives, we need to know it more on a small...

A secondary cervical thymus (CT) is present in the neck region in about 50% of human and mice. CT in mice is an independent and functional organ, which can be colonized by T lymphocyte progenitors and generate thymocytes that are selected by the T cell receptor repertoire following the positive and negative selection. However, CT and the main thora...

Impressão 3D de um modelo de traqueia utilizando material biocompatível e biodegradável para a engenharia tecidual. Com os avanços da biofarbicação, poderemos num futuro próximo, produzir tecidos biológicos funcionais. Quer saber mais!!! Encontre mais conteúdo no site de divulgação científica www.biofabricacao.com

Abstract Computer Simulation for biological and bioengineering purposes is being referred today also as In silico as a first approach for the In Vitro and In Vivo expensive and time-consuming tests. It is a necessary and fast-growing field of interdisciplinary knowledge to fulfill today's and future demands, ranging from bioengineering modeling and...

This review proposes to present how materials at nanolevel scale can contribute to the development of three-dimensional (3D) structures, human tissues, and organs which have macrolevel organization. Specific nanomaterials such as nanofibers and nanoparticles are presented and discussed in their application for biofabricating 3D human tissues and or...

Osteogenesis is a biological process involving the specification of mesenchymal stem cells and their maturation and differentiation into osteoblasts and osteocyte cells. Complex regulatory networks are required during these events in osteogenesis, and their imbalance can cause disastrous disordering and consequently diseases, such as cancer. Bone-r...

Métodos de cultivo celular Cultivo de células em 3D Moléculas de adesão Matrix extracelular Produção de esferoides Diferenças entre esferoides e organoides

Perspectivas do uso de esferoides para a bioimpressão e biofabricação de tecidos e órgãos.

Production of scaffolds using 3d printing technology

Scaffolds are 3D matrices capable of functioning as a 3D substrate for the cells and physical support for the development of a new tissue. This is the most commonly used strategy in tissue engineering and relies heavily on materials and production processes. These scaffolds can be produced through various techniques.

The micro-molds were created at Renato Archer Center for Information Technology. First of all, the molds were designed using Rhinoceros software generating a Stereolithographic (STL) file. After that, the micro-molds were materialized by additive manufacturing (AM) or also 3D printing. The STL file was carried out into the 3D printer machine. The A...

The purpose of this work is to present different approaches to predict the development and behavior of several biological processes, such as molecular networks, gene interactions, diffusion, cell differentiation, tissue and organ development, beyond to provide new perspectives and strategies in the biofabrication of tissues and organs. Results and...

Statement of Purpose:3D bioprinting process can be adapted to produce tissues in a variety of formats, structural complexities, such as material types, cell types, growth factors and differentiation, extracellular matrix composition, mechanical properties, macro and microvasculatureand technical challenges associated with the creation of biomodelst...

A very complex design (blueprint) will be fundamental to reach a 3D bioprinted organ in the future. Since many biological and mechanical aspects are involved the project of an organ encompasses an over wide range of variables. In vitro and in vivo experiments require the investment of large sums of money and time, besides being specific and complex

O que é Biofabricação de órgãos? A construção automatizada de produtos biologicamente funcionais com organização estrutural de células vivas, biomoléculas, biomateriais e agregados celulares, por meio da bioimpressão 3D e subsequente maturação em biorreatores. Objetivos • Produzir microtecidos e organoides para testes e desenvolvimento de drogas; •...

BOOK - Recent Trends in Cancer Biology: Spotlight on Signaling Cascades and microRNAs Editors: Fayyaz, Sundas, Farooqi, Ammad Ahmad (Eds.) Cancer is a multifaceted disease and overwhelmingly increasing experimental evidence has helped us to develop a deeper understanding of the role of signal transduction cascades in cancer development and progres...

3D bioprinting process can be adapted to produce tissues in a variety of shapes, structural complexities. In recent years, in silico approach, has been practiced in several fields, and offers new opportunities for medical investigation and discovery, help in and improving the generation of new data and the storage, organization, and classification...

CTI) janaina.dernowsek@cti.gov.br Resumo Avanços na manufatura aditiva têm permitido a impressão 3D de materiais biocompatíveis, células e componentes de suporte em alguns tecidos funcionais. O processo de bioimpressão 3D pode ser adaptado para produzir tecidos em uma variedade de formatos e complexidades, além de obter específicas propriedades bio...

3D bioprinting process can be adapted to produce tissues in a variety of shapes, structural complexities. In recent years, in silico approach has been prac­ticed in several fields, and offers new opportunities for medical investigation and discovery, helping and improving the generation of new data and the storage, organization, and classification...

http://profissaobiotec.com.br/biofabricacao-de-orgaos-um-campo-multidisciplinar/ O que antes era apenas ficção científica, hoje é realidade e um dos temas multidisciplinares mais recorrentes na mídia internacional e brasileira. Ainda não temos órgãos funcionais "bioimpressos", mas a tecnologia para essa realização já está sendo aprimorada e em pl...

Este trabalho propõe um blueprint interdisciplinar para a biofabricação de órgãos que utiliza as ideias comuns de simulações - como métodos probabilísticos preditivos e cálculos de energia - aplicados a dois frameworks: o mecânico e o biológico. Em resumo, o mecânico utilizaria a análise de elementos finitos para observar comportamentos fluidodinâ...

1. O que é Biofabricação 2. Simulação computacional na Biofabricação 3. Vantagens e Desvantagens 4. A Simulação como ferramenta de suporte à decisão 5. Por que Simular? Quando Simular? 6. Métodos usados para simular 7. Aplicações na Engenharia Tecidual

Tissue maturation - Bioreactors The third step, 3D tissues are subject to controlled physical and chemical conditions so that the living material has time to undergo cell differentiation. It is a maturation period that precedes the in vivo implantation of the solution. The equipment that guarantees this process is known as a bioreactor. YouTube - h...

The biofabrication line comprehends mainly of the following equipment: cell sorters, microfluidic devices, tissue spheroid fabricator and encapsulator, bioprinter, bioreactor among others, as can be seen in this video. YpuTube --> https://www.youtube.com/watch?v=-xPAcX5yVgk&list=PLs_Es7-NwvIwlyt0T0ueJFJE_hJIcZj6O

The bioprinting step is an emerging variant of the biomedical application of additive manufacturing or 3D printing. This step depends on the other aspects, such as the design of the organ (BioCAD) and the biomaterial for processing. YouTube --> https://www.youtube.com/watch?v=ok5EjiFZ5_Q&list=PLs_Es7-NwvIzpxcrHw3VprEzMQQZalVoB&index=3 - http://www...

Just like any product one would like to manufacture, a bioprinted organ also needs a project. In biofabrication, this project is named blueprint. Being a biological product, in addition to the physical properties, the project of an organ encompasses a wider range of variables. In this regard, tissue engineering assists in the modeling of biological...

Introduction: Nowadays, 3D bioprinting processes can be used to produce biocompatible products in a variety of formats, structural complexities, based on different properties and materials, cell types, growth factors, and differentiation stage. Moreover, the extracellular matrix composition, mechanical properties, macro and microvasculature, and th...

Cell Signaling Pathways and microRNAs in Cancer Biology - Editors: Fayyaz, Sundas, Farooqi, Ammad Ahmad (Eds.) Features cutting-edge coverage and translational perspective; Provides mechanistic insights and most recent ‘bench to bedside and back’ breakthroughs; Different chapters of the book are written by an international panel of researchers, sp...

3D bioprinting process can be adapted to produce tissues in a variety of shapes, structural complexities. In recent years, in silico approach has been prac­ticed in several fields, and offers new opportunities for medical investigation and discovery, helping and improving the generation of new data and the storage, organization, and classification...

Simulação Computacional na Engenharia de Tecidos - minicurso apresentado no 14° Congresso da Sociedade Latino Americana de Biomateriais, Orgãos Artificiais e Engenharia de Tecidos – SLABO / 5ª Edição do Workshop de Biomateriais, Engenharia de Tecidos e Orgãos Artificiais – OBI (20/08/2017) Maresias-SP Tópicos apresentados: O que é simulação computa...

Assim como qualquer produto que deseja-se fabricar, um órgão também precisa de um projeto - seu blueprint. Aqui, ele é proposto na forma de simulações in silico utilizando ferramentas CAD e CAM integradas a modelos probabilísticos preditivos e cálculos de energia por meio de dois frameworks: o mecânico e o biológico

Biofabrication as an interdisciplinary area is fostering new knowledge and integration of areas like nanotechnology, chemistry, biology, physics, materials science, control systems, among many others, necessary to accomplish the challenge of bioengineering functional complex tissues. The emergence of integrated platforms and systems biology to unde...

Disponível: http://www.3dprinting.com.br/entrevistas/janaina-dernowsek-um-panorama-da-bioimpressao-3d-no-brasil/ Janaina Dernowsek é pesquisadora e autora de artigos científicos envolvendo uma área do conhecimento pouco difundida no Brasil: a biofabricação. Em nossas andanças por aí em busca de pautas sobre bioimpressão, eis que somos agraciados co...

Palestra ministrada para os cursos de Ciências Biomédicas e Informática Biomédica da USP.

PALESTRA SOBRE BIOFABRICAÇÃO REALIZADA DURANTE A VII Jornada de Sistemas Biomédicos- SisBio 2017 EM SOROCABA - SP, no dia 07/06/2017

Investigation on functional genome research may contribute to the knowledge of functional roles of different mRNAs and miRNAs in bone cells of osteoporotic animals. Currently, few studies indicate the changes in gene modulation that osteoporosis causes in osteoblastic cells from different sites. Thus, the purpose of this investigation was to evalua...

O termo Biofabricação tem sido amplamente usado por uma diversidade de áreas do conhecimento, e reavaliado em artigos científicos recentes, entretando, o conceito mais atual e aceito pela sociedade científica é ”a construção automatizada de produtos biologicamente funcionais, com organização estrutural de células vivas, biomoléculas, agregados celu...

La fabricación por manufactura aditiva (MA) ha sido investigada y desarrollada desde los últimos 20 años. Esto ha conllevado también al avance de otras áreas como la ingeniería de tejidos. La novedad y la ventaja de estas técnicas recaen en el hecho de que en lugar de fundamentar el proceso en la eliminación de material, ésta crea piezas tridimensi...

We demonstrate that the interaction between miR-450a-5p and miR-28-5p and signal transducer and activator of transcription 1 (STAT1) mRNA correlates with the osteoblastic differentiation of mesenchymal stem cells from human exfoliated deciduous teeth (shed cells). STAT1 negatively regulates runx-related transcription factor 2 (RUNX2), which is an e...

Review Information technology (IT) is ubiquitous in recent human existence. The aim of this article is to present some basic concepts and specific demands that biofabrication may place on IT. Some of these technologies are already available, with a need for improvement, while others will need to be newly developed. Technologies that clearly, precis...

Aggregates of cells form structures called tissue spheroids (TS) that have been widely used in the field of tissue engineering. The contact of the cells with the culture medium is related to cell viability and to the increase of proliferation rate. Nutrition, organization and growth in cells are largely determined by diffusion mechanisms. Due to th...

Cardiovascular disease remains as one of the main problems in contemporary health care worldwide. Several studies of the cardiac prostheses have been held since 60s with the advent of cardiopulmonary bypass. The mechanical properties of blood vessels, arteries and valves depend on collagen and elastic fibers, as well as on smooth muscle cells and g...

Understanding the tissue growth and predicting the mechanical properties of the prosthetic valves, will be a giant leap forward in optimizing the designs of tissue-engineered heart valves that will last for a lifetime.

Multi scale systems - is sort of an intrinsic part of the study of systems biology • Molecular components to sbcellular machines – transcriptional machinery, cell motility machinery • subcellular machines to cells • Cells to tissues and organs • Organs to whole organisms Increasing levels of organization give rise to new properties and capabilities