Jana Ziarovska

Slovak University of Agriculture · Department of Genetics and Plant Breeding (KGŠR)

The BBAP analysis (Bet v 1 based amplified polymorphism) used in the study was aimed to detect natural variation among thirteen samples of Golden Delicious and Granny Smith apple cultivars. These cultivars were selected due to their global commercial importance and their documented association with high concentrations of the allergen Mal d 1. All o...

The genetic variability in 13 selected genotypes of Vaccinium corymbosum using re-trotransposon-based iPBS markers system was characterized in this study. The four selected iPBS primers amplified 232 fragments, average 58 fragments per primer, approximately 53.16% of which were polymorphic. The polymorphism information content of iPBS marker was 0....

Medicinal plants have been a part of human life from a very early age. In the field of plant genetics, they are still widely investigated for their genomic variability. This study used two DNA marker techniques to obtain polymorphic profiles in selected species from Lamiaceae. Both are based on the variability of plant genes that code for allergens...

Apples are a popular fruit worldwide, with many health and nutritional benefits. However, this fruit is also among those that, particularly in Central and Northern Europe, are allergenic due to the Mal d 1 allergen. Mal d 1 is a homologous allergen to Bet v 1—the main pollen allergen of birch. In this study, two different approaches were used to id...

During evolution, plants have developed complex mechanisms to overcome biotic and abiotic stresses. Studying the interactions between heavy metals and plants, especially at the molecular-genetic level, helps to understand the accumulation of heavy metals in plants and their resistance to heavy metal-induced stress. The impact of cadmium toxicity of...

Nonspecific lipid transfer proteins (nsLTP) are common allergen discovered and described 40 years ago. They are present in many plant species and protect plants from stressors such as heat and drought. Studies on the peach nsLTP, Pru p 3, show that nsLTP are very cross-reactive trough presented IgE epitopes shared by nsLTP from other botanically re...

Frosty pod rot (FPR) disease, caused by Moniliophthora roreri, is the most important cocoa disease in the Western Hemisphere. In Guatemala, the presence of the pathogen is attributed to a rapid and clonal dissemination throughout Central America after one or very few introductions of M. roreri from South America. We analyzed the genetic diversity o...

Physiological and molecular marker-based changes were studied in the tissues of two-year-old Pyrus pyraster (L.) Burgsd. seedlings under salt treatment. For 60 days, 5 mL of 100 mM NaCl solution was applied to each plant per day to a cumulative volume of 300 mL in the substrate. In response to osmotic stress, the seedlings increased their water use...

In Poland, the mountainous and hilly regions until recently were territories with unbalanced and poor diet quality, especially in early spring. Wild edible plants were or remain influential in supplementing a human diet. One of the earliest wild edible plants to appear in the growing season, characteristic of a broad swath of Europe from Ukraine to...

Melissa officinalis L., a well-known herb with diverse industrial and ethnopharmacological properties. Although, there has been a significant lack in the breeding attempts of this invaluable herb. This study aimed to enhance the agronomical traits of M. officinalis through in vitro polyploidization. Nodal segments were micropropagated and subjected...

The aim of research was to verify variability of selected parameters of hygienic quality of chickpea spreads in relation to the used ingredients and storage conditions. Four basic groups of chickpea spreads (A, B, C, D) contained the same ingredients (boiled chickpea, salt, garlic). The decisive factor for assessing their hygienic quality was the a...

In Poland, the mountainous and hilly regions until recently were territories with unbalanced and poor diet quality, especially in early spring. Wild edible plants were or still are influential in supplementing a human diet. One of the earliest wild edible plants to appear in the growing season, characteristic of a broad swath of Europe from Ukraine...

The present study aimed to document the traditional use of medicinal plants used to treat Diabetes mellitus Type II in Bolivia. Based on the scientific literature were identified 35 medicinal plant species distributed in 21 botanical families, of which 52 % are native to Bolivia and 48 % are introduced. The botanical families with the highest repre...

Arachis hypogaea L. – peanut (syn. groundnut) belong botanically to annual herbs, more specifically to legumes (family Fabaceae). Cultivated from is characterized as allotetraploid specie and that has a large and genome with high level of complexity as a result of natural hybridization of wild diploid species Arachis cardenasii (nn) and Arachis bat...

Background. For the Guatemalan culture, the avocado ( Persea americana Mill) represents more than a crop, since it has religious, mythical, economical, and medicinal significance typical of the center of domestication of the species. Although there is speculation about the wide variability of wild avocado germplasm, there are no studies describing...

Cadmium (Cd) is a heavy metal that can cause damage to living organisms at different levels. Even at low concentrations, Cd can be toxic to plants, causing harm at multiple levels. As they are unable to move away from areas contaminated by Cd, plants have developed various defence mechanisms to protect themselves. Hyperaccumulators, which can accum...

Avena sativa L. is a very common specie for human consumption that provide different health benefits. As a dual purpose crop, oat provide a source of protein, fiber and minerals. Here, homologs of Bet v 1 allergen genes were used as DNA markers to analyse their utilization for genomic variability of oat varieties. The presence of Bet v 1 homologues...

Drought susceptible (Aladin, Dagmar) and drought tolerant (Seladon, Venturero) varieties of summer wheat were examined in experiment. Genotypes were tested under In vitro conditions on Murashige-Skoog culture medium with different concentrations (0, 5, 10, 15 and 20 %) of polyethylene glycol to induce dehydration stress conditions. Genomic response...

This study was designed to describe bacterial profiles of ejaculates collected following a long and short ejaculatory abstinence set in the context of changes in the conventional, oxidative, and immunological characteristics of semen. Two specimens were collected in succession from normozoospermic men (n = 51) following 2 days and 2 h, respectively...

This study aimed to characterize the bacterial profiles and their association with selected semen quality traits among two chicken breeds. Thirty Lohmann Brown and thirty ROSS 308 roosters were selected for semen quality estimation, including sperm motility, membrane and acrosome integrity, mitochondrial activity, and DNA fragmentation. The oxidati...

Melissa officinalis L. is well known for its lemon-scented aroma and various pharmacological properties. Despite these valuable properties, the genes involved in the biosynthetic pathways in M. officinalis are not yet well-explored when compared to other members of the mint family. For that, gene expression studies using quantitative real-time PCR...

Apples are popular fruit worldwide thanks to their many tastes and a nutritional value. On the other side, they share the allergenic epitopes of Mal d 1 allergen that is relevant in food allegies. Sharing the genetic information of the origin accelerated the crossbreeding of world-class genotype in terms of the genetic resource of different apple s...

A plant microbiome is an important factor in plant growth, stress resistance, health status, and consumer quality and safety. The rhizosphere microbiome evolves in a negotiation between microbial communities that inhabit soil and plant root tissue. In this study, the rhizosphere and root internal tissue microbiome of six varieties of lettuce were a...

The aim of the study was to analyse selected aspects of the natural variability of selected varieties of Vitis vinifera. Grapevine is one of the most popular and desirable crops in the world due to the great tradition of wine production, but grape extracts also have a wide range of pharmaceutical effects on the human body. It is important to identi...

Growers are trying to meet the requirements for low tolerance of nitrates in cultivated crops through appropriate management of cultivation practices, paying attention to the timing, speed and form of nitrogenous substances used. However, external conditions during crop development are also crucial, such as light intensity and quality or air temper...

In Guatemala, potato crop production is affected by the nematodes Globodera rostochiensis and Globodera pallida. The ability of both species to form cysts complicates their control and causes an increase in their populations. In Guatemala, both species of nematodes have been reported by morphological identification; however, molecular confirmation...

Since the molecular similarities and differences among physiological capacitation and cryocapacitation have not been studied in detail, this study was designed to assess the gene and protein expression levels of the Cation channel of sperm (CatSper) 1 and 2, sodium bicarbonate (Na+/HCO3−) cotransporter (NBC) and protein kinase A (PKA) in un-capacit...

This study served to elucidate the effects of three selected doses of epicatechin (EPI; 25, 50 or 100 μmol/L) on the motion and oxidative profile of bovine spermatozoa subjected to the process of cryopreservation. Furthermore, we focused on the impact of EPI on the expression patterns of heat shock proteins 90 and 70 as well as the BAX and Bcl-2 pr...

The objective of the current study was to assess the efficiency of oryzalin in inducing polyploids in Callisia fragrans (Lindl.) Woodson by in vitro polyploidization. Shoot tips were subjected to Murashige and Skoog (MS) medium containing oryzalin at concentrations 1, 5, and 10 μM for 4 and 8 weeks. Further, the ploidy levels of the plants were con...

The nuclear reactor accident in Chernobyl, Ukraine, resulted in effects both locally and farther away. Most of the contaminated areas were the agricultural fields and forests. Experimental fields were established near Chernobyl—radioactively contaminated fields localized 5 km from Chernobyl Nuclear Power Plant as well as the remediated soil that is...

This study focused on the identification of bacterial profiles of semen in normozoospermic men and their possible involvement in changes to the sperm structural integrity and functional activity. Furthermore, we studied possible fluctuations of selected cytokines, oxidative markers, and antibacterial proteins as a result of bacterial presence in th...

Here, the specific natural variability of iPBS (Inter Primer Binding Sites Polymorphism) fingerprints in thirteen varieties of Vitis vinifera, L. was performed. All of the analysed biological material was collected in the vineyard of Sabo vinery for describing of the existing genetic polymorphism. Young leaves from a total of thirteen grapevine var...

Even though Guatemala is considered one of the three domestication centres of the avocado (Persea americana Mill), little is known about the genetic diversity of its native populations. This is the first study to explore avocado genetic diversity and population structure in native dispersal areas using microsatellite markers. We sampled 189 avocado...

Oyster mushroom (Pleurotus ostreatus) is still one of the most cultivated edible and medicinal mushrooms. Despite its frequent cultivation around the world, there is currently just a little information available on the variability of strains in terms of the content of β-glucans in them. This work presents an extensive study of 60 strains in terms o...

PR-10 is an ubiquitous part of plant proteomes that are reported as homologues proteins to the Bet v 1, that was found previously to be a major birch-pollen allergen. In many plant species, PR-10 proteins are involved in allergic cross-reactions of sensitive patients. Despite their clinically relevance, PR-10 proteins are still not reported and cha...

Bacterial contamination of semen is an often overlooked, yet important, factor contributing to decreased sperm vitality. Understanding the impact of bacterial presence on sperm structural integrity and functional activity may assist the development of effective strategies to prevent, or manage, bacteriospermia in the breeding practice. The aim of t...

Phosphorus (P) is an essential element in agricultural production, but due to its complex dynamics in the soil, only a tiny amount is usable by plants. This is because most P is in insoluble forms, especially in volcanic Andisol soils. Microorganisms with phosphorus solubilizing capacity (MSF) are an alternative for transforming P into soluble form...

The significant healing effect of Tilia platyphyllos Scop. and Tilia cordata Mill. flowers are well known. However, the flowers of Tilia tomentosa Moench. are not suitable for harvest due to their toxic effects. To investigate the diverse background of this effect, we applied a functional miRNA-based marker, mannose expression analysis and determin...

STORAGE OF MARINATED MEAT INTENDED FOR GRILLING FROM THE FOOD SAFETY POINT OF VIEW Lucia Zeleňáková, Jana Žiarovská, Anna Kolesárová Abstract: The aim of the work was to evaluate selected aspects of food safety and quality of fresh and marinated poultry meat intended for grilling. We focused on microbiological and physico-chemical analysis of fresh...

Vitis vinifera L. is a well-known cultural plant, which is grown on thousands of hectares throughout the world. However, it has the potential to cause allergic reactions. Even allergy to grapes is not a common allergic reaction, the prevalence of this type of allergy is growing, particularly in southern Europe countries. Here, we focused on the dif...

Bacterial contamination of semen is an important factor connected to the health status of bulls that may significantly affect semen quality for artificial insemination. Moreover, some important bovine diseases may be transmitted through semen. Up to now, only a very limited number of complex studies describing the semen microbiome of bulls have bee...

Different types of allergies became a part of life of many people around the world. The research activities connecting to allergens are actually not oriented only for protein and immunological interactions, but to the genomic and transcriptomic background of them, too. Analysis and description of genomic variability of allergens in plant food resou...

Several types of allergies are currently known and are characterized by an exaggerated response of the immune system to substances from various sources called allergens. One of them is a food allergy, which is becoming more common in the population. For this reason, it is necessary to describe the issue from several aspects including genomic variab...

Although bacterial contamination of ejaculates may cause difficulties in cattle reproduction, standard protocols for a routine microbiological analysis of bovine semen are still missing. Understanding of the mechanisms of bacterial damage to spermatozoa may contribute to the prevention and management of bacteriospermia in the future. Therefore, thi...

Nowadays, various approaches for the precise DNA methylation analysis are known. This study applied an advanced MethyLight approach for precise detection of cytosine methylation. Chernobyl area is still contaminated by long-lived radioisotopes thus the potential risk of DNA damage by radiation remains relatively high. Plants as sessile organisms ar...

Pollen allergies are an important and relevant question in the personal life of millions of people. They are well characterized on the genomic and proteomic level of allergens per se and on medicinal level, but the very few information exists about the natural variability and background of pollen allergen transcription patterns. Here, the variabili...

The aim of study was to determine the antimicrobial activity of ten essential oils against ten microorganisms isolated from fish. The following essential oils were tested: Cymbopogon nardus Rendle, Citrus limon, Leptospermum petersonii Bailey, Gaultheria procumbens, Pelargonium graveolens, Citrus paradisi, Citrus aurantifolia, Litsea cubeba Pers.,...

This study was designed to investigate chemical and antioxidant properties, as well as the antimicrobial and antibiofilm behaviour of Cinnamomum cassia essential oil (CCEO). MALDI-TOF MS Biotyper mass spectrometry was applied to evaluate the biofilms of Stenotrophonomonas maltophilia and Bacillus subtilis, while the antibiofilm ability of CCEO was...

Plants are considered one of the toughest organisms in the world because of their ability to survive under the harsh conditions. Chernobyl offers a unique environment to study plant adaptation. Two experimental fields were established in the area near Chernobyl to closely study the effect of radioactive contamination on flax. Radioactivity severely...

Bet v 1 is a highly immunogenic protein, which is the main cause of sensitivity to birch pollen and is described as the main birch allergen. Despite the structural similarity, Bet v 1 homologs show different properties and immunoreactivity. Here, the bioinformatic algorithms were applied for known Bet v 1 homologous nucleic acids sequences to find...

Staphylococcus spp. is not only a commensal bacteria but also a major human pathogen that causes a wide range of clinical infections. Recent evidence suggests that Staphylococcus has the ability to colonize the reproductive system and to affect its structure and functions. The objective of this study was to determine the chemical properties and ant...

El aguacate es un cultivo de gran consumo a nivel mundial, localizándose su lugar de origen en la región de la Sierra Nevada en California hace ocho a cinco millones de años. En Guatemala se ha reportado una alta diversidad genética mediante caracterizaciones morfológicas, pero debido al comportamiento de polinización cruzada e hibridaciones interr...

The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasi...

Phosphorus (P) is an essential element in agricultural production. However, due to its complex dynamics, only a small portion of the total present in the soil can be used by plants. This is because most are found in insoluble forms, especially in Andisol soils of volcanic origin. Phosphate solubilising microbes (PSMs) are an alternative to transfor...

The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of Citrus aurantium essential oil (CAEO). The biofilm profile of Stenotrophonomonas maltophilia and Bacillus subtilis were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of Citrus auran...

The aim of this study was to compare the natural variability of the expression of class I chitinase gene in grapes of Vitis vinifera L. varieties as well as investigate their antimicrobial activity. Further, the effect of antimicrobial activity in chitinase expression was analyzed. Matured berries of thirteen grape varieties were used in the study—...

Work aimed to investigate the antimicrobial activity of medicinal plants against selected species of food industry pathogens in vitro conditions. The detection of antibacterial properties was examined by the disc diffusion method and the method of the minimum inhibitory concentrations (MIC). The cultivation of microorganisms after the 24 h was perf...

Recent studies reported a significant contribution of microRNAs (miRNAs) in plant response to elicitors. The aim of our study was to analyse the effect of exogenously applied plant-derived elicitor cobalt-diglycyrrhizinate on Triticum aestivum L. genome based on the functional characterization of stress associated miRNAs. Our results confirmed the...

This study aimed to analyze the genetic diversity of selected cultivars and clones of Lonicera sp. (edible honeysuckle) from two countries with different climatic and soil conditions. Accordingly, the determination of total polyphenols content (TPC) and total antioxidant activity (TAA) in fruits of Lonicera sp. in 2018–2019 was performed. Applied s...

Ionizing radiation in environment comes from various natural and anthropogenic sources. The effect of radioactivity released after the CNPP (Chernobyl Nuclear Power Plant) on plant systems remains of great interest. Even now, more than three decades after the nuclear accident, the long-lived radionuclides represent a strong stress factor. Herein, t...

The aim of this study was to identify lactic acid bacteria (LAB) in grapes, must and wines. A total amount of 90 samples including grape ( n = 30), must (no = 30) and wine (no = 30) were collected from vineyards in Slovakia. LAB were used cultured on MRS agar with subsequent confirmation with MALDI-TOF mass spectrometry (Bruker Daltonics). Altogeth...

Vitis vinifera L. is adapted to a very variable range of climates but it mostly grows in the temperate regions of continental Europe. In the Slovak Republic, the Tokaj wine region is one of the territories, where wine production is concentrated. Grape is a popular fruit and when processed, it is used as musts, juices or wine. Some people may suffer...

Phytoremediation efficiency depends on the ability of plants to accumulate, translocate and resist high levels of metals without symptoms of toxicity. This study was conducted to evaluate the potential of grain amaranth for remediation of soils contaminated with Cd. Three grain amaranth varieties, “Pribina” (A. cruentus), “Zobor” (A. hypochondriacu...