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Jana Ambrožič-Dolinšek

Jana Ambrožič-Dolinšek
University of Maribor, Faculty of natural sciences and mathematics and Faculty of education, Slovenia · Department of Biology and Department of primary education

Assistant Professor, PhD

About

29
Publications
12,574
Reads
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247
Citations
Additional affiliations
December 1990 - present
University of Maribor
Position
  • Professor (Assistant)
Description
  • Development of in vitro procedures for protection, micropropagation, storage and cryopreservation of endangered, rare or voulnerable wild species. Investigation of socio-scientific issues from the field of biotechnology.
December 1990 - present
University of Maribor
Position
  • Professor
Description
  • University teacher of Plant Physiology, several subjects from the field of Plant Physiology, Plant Biotechnology and Natural Sciences – Biology.
Education
September 1990 - October 2003
University of Ljubljana
Field of study
  • plant biotechnology, tissue culture, micropropagation, secondary methabolism

Publications

Publications (29)
Article
Regeneration fromPlatycerium bifurcatumscalesin vitrowas observed for the first time. Regeneration was obtained from scales detached fromin vitrogrown juvenile shoots. Regeneration began as rhizoids or as a proliferation of one or more scale cells into undifferentiated outgrowths distinguishable from adjacent cells by their shape and colour. Outgro...
Article
Full-text available
The induction, origin, morphology, and ploidy of aposporous gametophytes produced on juvenile leaves of the fern Platycerium bifurcatum (Cav.) C. Chr. were studied. Leaf explants were grown on modified Murashige and Skoog medium with 0%, 0.01%, 0.1%, 1%, or 2% sucrose. A low sucrose concentration (0.01%) and wounding of the adaxial side of the leaf...
Article
The aim of the work was to infect pyrethrum (Tanacetum cinerariifolium (Trevir.) Schultz-Bip.), an important secondary metabolite pyrethrin producing plant, with MA phytoplasma (16S rRNA group III, subgroup B) to initiate tissue culture of phytoplasma-infected plants, and to examine possible effects of infection on pyrethrin content, morphology, an...
Chapter
Full-text available
The staghorn fern genus, Platycerium, is an epiphytic genus, consisting of 15–18 species cultivated worldwide because of their unique appearance. Its economic value is great, since it stands out among ferns as one of the most commonly grown ornamental ferns. In vitro studies of some Platycerium species have revealed its great morphogenetic potentia...
Article
The objective of this study was to investigate knowledge, opinions, and attitudes toward, as well as readiness to accept genetically modified organisms (GMOs) among prospective primary and secondary Slovene teachers. Our findings are that prospective teachers want to take an active role in rejecting or supporting individual GMOs and are aware of th...
Article
The application of highly porous and 3D interconnected microcellular polyelectrolyte polyHIPE (PE-PH) monoliths based on (3-acrylamidopropyl)-trimethylammonium chloride as soilless cultivation substrates for in vitro embryo culture is discussed. The embryo axes isolated from chickpea seeds are inoculated onto the surface of the monoliths and allowe...
Book
Priročnik je namenjen študentom na študijskih programih s področja biologije, ekologije z naravovarstvom in bodočim učiteljem biologije, ki bi se radi spoznali z eksperimentalnim delom s področja fiziologiji rastlin. Sestavlja ga osem vsebinskih sklopov, ki obravnavajo rastlinske pigmente, dihanje rastlin in fotosintezo, encimsko aktivnost rastlin,...
Article
Full-text available
H. pluvialis is a unicellular freshwater alga containing many bioactive compounds, especially carotenoids, which are the strongest antioxidants among the pigments. This study evaluates the composition and content of carotenoids and other pigments in both stages of algae life cycle, especially in the green vegetative stage, less studied in compariso...
Article
Full-text available
Haematococcus pluvialis, a unicellular green microalga that produces a secondary metabolite under stress conditions, bears one of the most potent antioxidants, namely xanthophyll astaxanthin. The aim of our study was to determine the content of astaxanthin and its esterified forms using three different solvents—methyl tert-butyl ether (MTBE), hexan...
Article
Full-text available
School-based learning has a long tradition in Slovenia and is now a globally recognized movement. We present the operation of school gardens in the most agricultural region of Slovenia, the Pomurje region, and their inclusion in the pedagogical process of science subjects. Half (19 out of 38) of the primary schools in Pomurje have school gardens. T...
Chapter
Znanstvena monografija o poučevanju slovenščine kot drugega in tujega jezika (DTJ) za otroke priseljence, ki so zaradi svojih jezikovnih in kulturoloških posebnosti v neugodnem položaju v primerjavi z vrstniki, je zasnovana transdisciplinarno in prinaša razprave s področja humanistike, družboslovja, naravoslovja in umetniških disciplin, ki se z raz...
Article
Full-text available
Tissue culture of Berula erecta is suitable cultivation system for research purposes connected with contamination and phytoremediation studies. In previous investigation we determined the optimal dose concentration at which Se stimulates plant growth and positively affects the antioxidative status in this experimental system. In current study, we i...
Article
Full-text available
The objective of the present study was the cryopreservation of monotypic endemic Hladnikia pastinacifolia Rchb. shoot tips from an in vitro culture, via encapsulation-dehydration (ED) or encapsulation-vitrification (EV). For all tested genotypes, the highest rates of shoot regrowth and multiplication were obtained after overnight preculture in 0.4...
Article
Full-text available
Macrophyte Berula erecta, grown in tissue culture, was exposed to various low concentrations of NaCl in the water (1-100 mg L-1). Added NaCl had a positive effect on plant's growth and development. The number of shoots increased, as well as the length of the roots. The lowest concentration (1 mg L-1) increased photochemical efficiency of photosyste...
Article
Studies are scarce regarding the physiological effects of selenium treatment on antioxidant capacity of plants. In the presented in vitro experiment, Apium repens was used to investigate its ability to absorb Se(IV) at different concentrations (0.01, 0.1, 1, 10 and 50 mg L⁻¹) and its physiological response to treatment. These plants possessed a hig...
Article
Full-text available
In the presented in vitro experiment, the effect of selenite treatment (0.01, 0.1, 1, 10 and 50 mg L−1 Se(IV)) on Berula erecta was investigated with respect to growth, photochemical efficiency, photosynthetic pigments, anthocyanins and low molecular weight thiols. Lower Se(IV) concentrations (0.01, 0.1 and 1 mg L−1) promoted growth, while higher S...
Article
Full-text available
The monotypic Hladnikia pastinacifolia Rchb. is a narrow endemic species, with an extremely small distribution area in Slovenia, prone to any kind of threat that could lead to species extinction. Tissue culture techniques are proposed as a conservation measure for rapid propagation and ex-situ conservation. Tissue culture was initiated from seeds a...
Article
Full-text available
In recent times exchange of students between countries became popular. As teacher educators we have to prepare our students to teach efficiently in fast changing world and to be effective in future careers with educational technologies not yet discovered. Because of mobility and especially fast development of information and communication technolog...
Article
Full-text available
Genetically modified organisms cannot be regarded as merely a topic for academic debate, since these have serious implications as a research field and for production based on genetic engineering. Public debates rarely base their arguments on elements rooted in scientific arguments and knowledge but are heavily loaded with emotions, opinions and inf...
Article
Full-text available
The objective of this study was to investigate knowledge about, opinions on and attitudes toward and finally readiness to accept genetically modified organisms (GMO) among Slovenian teachers. On average, they have higher levels of knowledge in classical genetics, and poor levels of knowledge about modern issues in biotechnology, and their attitudes...
Article
Full-text available
The objective of this study was to investigate knowledge about, opinions on and attitudes toward and finally readiness to accept genetically modified organisms (GMO) among Slovenian teachers. On average, they have higher levels of knowledge in classical genetics, and poor levels of knowledge about modern issues in biotechnology, and their attitudes...
Article
Full-text available
Pyrethrum [Tanacetum cinerariifolium (TREVIR.) SCHULTZ-BIP., Asteraceae] is a source of pyrethrins, natural insecticides that are widely used in agriculture and for domestic purposes. In callus cultures of pyrethrum microbial contaminants were observed routinely, therefore it was necessary to use antibiotics to maintain aseptic cultures. The antibi...
Article
Full-text available
A new and unique shoot regeneration from the scales of Platycerium bifurcatum is described, and the effect of 1, 2, or 3% sucrose on regeneration is studied. Scales detached from 3-5 month old plants grown in vitro on medium without growth regulators, were used as initial explants. After 30 days of culture, 45 to 60% of scales developed adventitiou...