Jan Teuber

Universität Heidelberg · University Clinic for Neurosurgery

Purpose Lynch Syndrome (LS) is a cancer-predisposing condition resulting from hereditary mutation of DNA mismatch repair genes. Gastrointestinal, urogenital, and endometrial carcinomas are well-known to predominantly occur in LS patients. In contrast, there are only few reports on brain tumours in the context of LS and to date intracranial tumour m...

Integrin-mediated cell adhesion and signaling is of critical importance for neuronal differentiation. Recent evidence suggests that an “inside-out” activation of β1-integrin, similar to that observed in hematopoietic cells, contributes to the growth and branching of dendrites. In this study, we investigated the role of the hematopoietic adaptor pro...

Evidence suggests that regulated ubiquitination of proteins plays a critical role in the development and plasticity of the central nervous system. We have previously identified the ubiquitin ligase Praja1 as a gene product induced during fear memory consolidation. However, the neuronal function of this enzyme still needs to be clarified. Hence, we...

Meningeal abnormalities such as dural ectasia are seen in Marfan syndrome, but spinal meningeal cysts are rarely seen. These cysts usually asymptomatic and often found incidentally on magnetic resonance imaging, large cysts may cause neurological deficits and pain secondary to nerve root compression. Case reports. Two patients with Marfan syndrome...

Evidence suggests that regulated ubiquitination of proteins plays a critical role in the development and plasticity of the central nervous system. We have previously identified the ubiquitin ligase Praja1 as a gene product induced during fear memory consolidation. However, the neuronal function of this enzyme still needs to be clarified. Here, we i...

Confirmation of a neuronal phenotype. PC12 cells of each stably transfected line were stained with antibodies against the neuron-specific markers beta3-tubulin (also called TuJ1; red) or MAP2 (red), each in combination with DAPI (cyan) and anti-alpha-tubulin (blue). (A) through (C) show the separate and merged stainings for cells overexpressing Pra...

Praja1-induced polyubiquitination and changes of NRAGE levels in COS-7 cells. Immunoblot analysis shows an increase of total protein ubiquitination and reduction of endogenous NRAGE in cells overexpressing HA-tagged Praja1.1 and Praja1.2 (lane 2) or HA-tagged Praja1.2 alone (lane 3) compared to mock controls (lane 1). At the same time, levels of NR...

Substrate independence of Praja effects. (A) Reduced growth of neurites of ≥25 µm is observed in cells overexpressing Praja1.1/2, regardless of the substrate used. (B) Cell rounding is reduced on all tested substrates except for collagen IV. (C) However, the overall proportion of differentiated cells, showing a neuron-like morphology, is generally...

Specificity of anti-Praja1 serum. The expression of both Praja1.1 and Praja1.2 from HA-tagged praja1.1 (lane 3) is detected in HEK293T cells using Praja-specific serum. The apparent molecular weight of ca. 95 kD and 65 kD, respectively, differs clearly from the predicted molecular weight of the two isoforms but is in agreement with the previously r...

Induction of apoptosis in COS-7 cells through Praja1 expression. (A) COS-7 cells acutely transfected with praja1.1 or praja1.2 display cell rounding and microspike formation (arrow), and develop pyknotic nuclei, indicating an induction of apoptosis. EGFP and HA fusion proteins are equally effective in inducing this phenotype (data not shown), where...

Supplemental experimental procedures. (DOC)