Jan Lorenzen

Jan Lorenzen
Danish Technological Institute · Chemistry and Biotechnology

About

71
Publications
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313
Citations

Publications

Publications (71)
Article
Full-text available
Background: The aim of this prospective study was to assess the diagnostic value of nuclear imaging with 18F-FDG PET/CT (FDG PET/CT), combined 111In-WBC/99mTc-Nanocoll, and 99mTc-HDP SPECT/CT (dual-isotope WBC/bone marrow scan) for patients with chronic problems related to knee or hip prostheses (TKA or THA) scheduled by a structured multidiscipli...
Article
Full-text available
Background A major perspective for the use of circulating tumor DNA (ctDNA) in the clinical setting of non-small cell lung cancer (NSCLC) is expected as predictive factor for resistance and response to EGFR TKI therapy and, especially, as a non-invasive alternative to tissue biopsy. However, ctDNA is both highly fragmented and mostly low concentrat...
Article
Full-text available
Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non‐healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds...
Article
Full-text available
The predominant indications for revision surgery after total hip (THA) or knee arthroplasty (TKA) are an aseptic failure (AF) and prosthetic joint infection (PJI). Accurate diagnosis is crucial. Therefore, we evaluated prospectively a multidisciplinary diagnostic algorithm including multi-modal radionucleid imaging (RNI) and extended microbiologica...
Article
Full-text available
Background: Unrecognized periprosthetic joint infections are a concern in revision surgery for aseptic failure (AF) after total hip (THA) or knee (TKA) arthroplasties. A gold diagnostic standard does not exist. The aim of the current study was to determine the prevalence of unrecognized periprosthetic joint infection (PJI) in a cohort of revision...
Article
Full-text available
Activating mutations in codon 12 and codon 13 of the KRAS (Kirsten rat sarcoma viral oncogene homolog) gene are implicated in the development of several human cancer types and influence their clinical evaluation, treatment and prognosis. Numerous different methods for KRAS genotyping are currently available displaying a wide range of sensitivities,...
Data
Example showing how to set the threshold when analyzing SensiScreen® real-time PCR data. The threshold is set at 10% of the normalized fluorescence signal of the reference assay at cycle 45 (black line). The threshold is subsequently used to read the Ct of the ref and the mutation-specific assay (mut). The difference in threshold cycle DCt is calcu...
Data
Cell lines used for sensitivity studies. *kindly provided by Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy. (PDF)
Data
MyGo Pro real-time PCR amplification plots of SensiScreen® KRAS exon 2 multiplex assays using serial dilutions of mutated DNA in a wild type background. 50 ng and/or approximately 16,000 copies of DNA was added to each reaction. The threshold was set at 10% of the average fluorescence signal of the reference assay at cycle 45. Legend describes the...
Data
Sequences of oligonucleotides used for construction of model templates for DS, ME-PCR and SensiScreen® assays. Proprietary modifications not shown. DS, direct sequencing; ME-PCR, mutant-enriched PCR. (PDF)
Data
Clinical-pathological patient data of cohort 3 used for analysis by cobas® KRAS mutation test and SensiScreen® multiplex. TNM, classification of malignant tumours (T, tumor; N, lymph nodes; M, metastasis); F, female; M, male. (PDF)
Data
KRAS exon 2 codon 12/13 mutational status of patients in cohort 1 determined by DS, mutant-enriched PCR ME-PCR and SensiScreen® simplex and multiplex. Mutated cases found only by SensiScreen® are underlined (sample 99) while cases found both by SensiScreen® and ME-PCR are in bold (n = 15). *As determined by both SensiScreen® simplex and multiplex....
Data
MyGo Pro real-time PCR amplification plots of SensiScreen® KRAS exon 2 simplex assays using serial dilutions of mutated DNA in a wild type background. 50 ng and/or approximately 16,000 copies of DNA was added to each reaction. The threshold was set at 10% of the average fluorescence signal of the reference assay at cycle 45. Legend describes the fr...
Data
Clinical-pathological patient data of cohort 1 used for analysis by direct sequencing, mutant-enriched PCR and SensiScreen® simplex and multiplex. TNM, classification of malignant tumours (T, tumor; N, lymph nodes; M, metastasis); F, female; M, male. (PDF)
Data
Clinical-pathological patient data of cohort 2 used for analysis by direct sequencing, therascreen® KRAS test and SensiScreen® simplex (patient 1–79). TNM, classification of malignant tumours (T, tumor; N, lymph nodes; M, metastasis); F, female; M, male. (PDF)
Data
KRAS exon 2 codon 12/13 mutational status of patients in cohort 3 determined by cobas® and SensiScreen® simplex and multiplex. Mutated cases identified by SensiScreen® but not by cobas® are underlined (sample 5, 53, 131, 170, 189, and 214). n, number; WT, wild- type. (PDF)
Data
KRAS exon 2 codon 12/13 mutational status of patients in cohort 2 determined by DS, therascreen® and SensiScreen® simplex and multiplex. Mutated cases found both by SensiScreen® and therascreen® are in bold (sample 59+70). n, number; DS, direct sequencing; WT, wild- type; NE: not evaluable. (PDF)
Article
Medical device-related infections cause undue patient distress, increased morbidity and mortality and pose a huge financial burden on healthcare services. The pathogens are frequently distributed heterogeneously in biofilms, which can persist without being effectively cleared by host immune defenses and antibiotic therapy. At present, there is no ‘...
Chapter
Traditional culture-dependent methods and a number of culture-independent molecular methods including 16S rRNA gene polymerase chain reaction, construction of clone libraries, sequencing, phylogeny, fingerprinting, fluorescence in situ hybridization and quantitative PCR were used to describe the microbial composition of two types of biofilm-related...
Article
Formation of biofilm is a prominent feature of prosthetic joint infections (PJIs) and constitutes a challenge to current sampling procedures and culture practices. Molecular techniques have a potential for improving diagnosis of biofilm-adapted, slow-growing and non-culturable bacteria. In this exploratory study we investigated the bacterial divers...
Article
Full-text available
During inspection of AISI316 stainless steel plate heat exchangers in a district heating peak load unit, localised corrosion attacks along with indications of microbiological activity were found on the boiler side beneath patches of sturdy black deposits. Bacteria and sulphide were detected within black deposits. Thorough investigation of the boile...
Article
Full-text available
High-resolution NO3- profiles in freshwater sediment covered with benthic diatoms were obtained with a new microscale NO3- biosensor characterized by absence of interference from chemical species other than NO2- and N2O. Analysis of the microprofiles obtained indicated no nitrification during darkness, high rates of nitrification and a tight coupli...
Article
Full-text available
Although O2 microsensors used for study of benthic photosynthesis have tip diameters of less than 10 pm and stem diameters of about 150 pm at a distance of 0.5 cm from the tip, they do change the local flow pattern in the overlying water and thereby reduce the thickness of the diffusive bound-ary layer (DBL). The reduced DBL resulted in a lower dif...

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