Jan Kopcewicz

Jan Kopcewicz
Nicolaus Copernicus University | umk · Chair of Plant Physiology and Biotechnology

About

155
Publications
14,099
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1,427
Citations
Citations since 2016
35 Research Items
504 Citations
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
Introduction
Skills and Expertise

Publications

Publications (155)
Chapter
The timing of the transition from vegetative to reproductive development is of great fundamental and practical interest. Physiological event is a subject to strict and precise control. Among the environmental factors, the most important is photoperiod defined as the following changes in the light and darkness duration in the daily cycle, while the...
Article
The abscission of plant organs is a phytohormone-controlled process. Our study provides new insight into the involvement of gibberellic acid (GA3) in the functioning of the flower abscission zone (AZ) in yellow lupine (Lupinus luteus L.). Physiological studies demonstrated that GA3 stimulated flower abortion. Additionally, this phytohormone was abu...
Article
This paper presents a comprehensive study on the mechanisms of tree responses to pathogens. We describe natural resistance concerning the presence of different types of barriers protecting plants from invasion and also give them strength after a pathogen attack. We put emphasis on induced resistance functioning both locally and systemically and inv...
Article
Full-text available
The involvement of gibberellins (GAs) in the control of flower induction in the short-day plant Ipomoea nil has been investigated. To clarify the molecular basis of this process, we identified the full-length cDNAs of the InGA20ox3 and InGA2ox1 genes, which encode enzymes responsible for GA biosynthesis and catabolism, respectively. We studied the...
Data
qPCR validation of differential expression patterns of selected unigenes. The relative quantification of the gene expression level was determined with qPCR assay using ACTIN as a reference gene. The values presented on top of each bar indicate the expression level derived from the RNA-Seq data.
Data
Annotation of L. luteus DEGs from an FAB vs. FNAB library comparison by KEGG database. (A) Distribution of DEGs into KEGG biological categories. The numbers of KEGG pathways belonging to a category are shown in brackets. (B) Classification of DEGs into the KEGG “Metabolism” category. The numbers of DEGs belonging to a pathway are shown.
Data
Annotation of L. luteus DEGs from an FPAB vs. FPNAB library comparison by KEGG database. (A) Distribution of DEGs into KEGG biological categories. The numbers of KEGG pathways belonging to a category are shown in brackets. (B) Classification of DEGs into the KEGG “Metabolism” category. The numbers of DEGs belonging to a pathway are shown.
Data
Statistical analysis of differentially expressed gene in compared libraries of L. luteus.
Data
Differential expression patterns of cell wall-related genes in library comparison of FAB vs. FNAB, FPAB vs. FPNAB, PAB vs. PNAB.
Data
Annotation of L. luteus DEGs from comparison of PAB vs. PNAB libraries by KEGG database. (A) Distribution of DEGs into KEGG biological categories. The numbers of KEGG pathways belonging to a category are shown in brackets. (B) Classification of DEGs into the KEGG “Metabolism” category. The numbers of DEGs belonging to a pathway are shown in bracket...
Data
Differential expression patterns of plant hormone signaling-related genes in library comparison of PAB vs. PNAB.
Data
List and annotation of differentially expressed genes (DEGs) between FAB and FNAB with PPDE < 0.05.
Data
The enriched pathways (KEGG) of DEGs from library comparison of FPAB vs. FPNAB.
Data
Quality filtering and statistics of raw reads obtained for eight transcriptome libraries of L. luteus.
Data
List and annotation of differentially expressed genes (DEGs) between FPAB and FPNAB with PPDE < 0.05.
Data
List and annotation of common DEGs identified in all three library comparisons of FAB vs. FNAB, FPAB vs. FPNAB, PAB vs. PNAB with FPKM (Fragment Per Kilobase of exon per Million fragments mapped) in each of the libraries.
Data
The enriched pathways (KEGG) of DEGs from library comparison of PAB vs. PNAB.
Data
List of primers used for the RT-qPCR validation and the RT-qPCR validation results.
Data
Differential expression patterns of plant hormone signaling-related genes in library comparison of FAB vs. FNAB.
Data
Differential expression patterns of plant hormone signaling-related genes in library comparison of FPAB vs. FPNAB.
Data
List and annotation of differentially expressed genes (DEGs) between PAB and PNAB with PPDE < 0.05.
Data
The enriched pathways (KEGG) of DEGs from library comparison of FAB vs. FNAB.
Article
Ent-kaurene oxidase (EKO) catalyze three sequential oxidations in the early steps of gibberellin biosynthesis pathway. In this research, a cDNA sequence of InEKO1 gene in the model short-day plant Ipomoea nil was identified. Our studies revealed that inductive conditions for flowering caused an increase in the transcriptional activity of examined g...
Article
Full-text available
Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant’s productivity. Generative organ detachment takes place at the basis of...
Article
The abscission of certain organs from the plant is part of the fulfilment of its developmental programs. The separation process occurs in a specialized abscission zone usually formed at the base of detached organ. The changing level of phytohormones, particularly ethylene, is the element responsible for coordinating anatomical and physiological tra...
Article
Full-text available
Interactions among jasmonates and auxin in the photoperiodic flower induction of a short-day plant Ipomoea nil were examined. Therefore, we measured changes in jasmonic acid (JA) and jasmonic acid methyl ester (JAMe) levels in the cotyledons of I. nil during the inductive night, as well as the effects of indole-3-acetic acid (IAA) on their content....
Article
Flower abscission is a highly regulated developmental process activated in response to exogenous (e.g. changing environmental conditions) and endogenous stimuli (e.g. phytohormones). Ethylene (ET) and abscisic acid (ABA) are very effective stimulators of flower abortion in Lupinus luteus, which is a widely cultivated species in Poland, Australia an...
Article
Full-text available
Jasmonic acid methyl ester (JAMe) has been recently shown to play a crucial role in many physiological processes. In this paper, we focused on cotyledon senescence in Ipomoea nil and revealed that JAMe and darkness are the main factors stimulating the process examined. What is more, we showed that mefenamic acid (a jasmonate biosynthesis inhibitor)...
Article
Full-text available
Allene oxide synthase (AOS) encodes the first enzyme in the lipoxygenase pathway, which is responsible for jasmonic acid (JA) formation. In this study we report the molecular cloning and characterization of InAOS from Ipomoea nil. The full-length gene is composed of 1662 bp and encodes for 519 amino acids. The predicted InAOS contains PLN02648 moti...
Article
Full-text available
Auxin affects almost all of the growth and development processes in plants. The auxin signal transduction pathway involves a number of proteins, among which the key elements are: TAAR auxin receptors (TIR1 and AFB1-3), AUX/IAA auxin response repressors and Auxin Response Factor (ARF) transcription factors. The activity of genes encoding some compon...
Article
Full-text available
The great agronomic potential of Lupinus luteus, species widely cultivated in many European countries as well as Australia, is strongly affected by premature and excessive generative organ abscission, mainly flowers. The unwanted process takes place in a specialized group of cells, called abscission zone (AZ). During their development they become c...
Conference Paper
Yellow lupine like the rest belonging to the family Fabaceae (Fabaceae L.), has important practical significance. Development of lupines flowers and fruit and are crucial for the productivity of these plants. Phytohormone auxin (IAA) plays an important role in the regulation of plant ontogeny, including apical dominance, the fruit maturation and ag...
Poster
Yellow lupine like the rest belonging to the family Fabaceae (Fabaceae L.), has important practical significance. Development of lupines flowers and fruit and are crucial for the productivity of these plants. Phytohormone auxin (IAA) plays an important role in the regulation of plant ontogeny, including apical dominance, the fruit maturation and ag...
Article
Full-text available
Twenty days-long stratification of Scots pine seeds results in the seeds germinating in similar high percentage in darkness as in light. The period of stratification enough to overcome the "barrier" of light is connected with simultaneous increase of gibberellin contents, especially with the appearance of the new groups of gibberellins qualitativel...
Article
Full-text available
Increasing the number of flowers and pods set, as well as reducing the intensity of their abortion, is of crucial importance for the yielding of leguminous plants. This study examined the effects of the type of soil used and mineral fertilization applied on the generative development of the traditional and self-completing (restricted branching) cul...
Poster
Yellow lupin (Lupinus luteus L.) is an important legume crop characterized by high content of seed proteins. The crucial stage for lupin productivity, the formation and development of flowers and seeds, is often associated with the abscission of flowers. Plant development processes are determined by a number of proteins and phytohormones, whose lev...
Article
Interactions between indole-3-acetic acid (IAA), abscisic acid (ABA), and ethylene (ET) in the photoperiodic flower induction of a short-day (SD) plant Pharbitis nil were investigated. It was shown that both IAA and ABA applied just before and during the first half of the 16-h-long inductive night inhibited flower induction in P. nil. Ethylene is a...
Article
Full-text available
It has been previously suggested that 1-O-IAGluc growth stimulation occurs as the effect of its hydrolysis into a free IAA. In present experiments castanospermine, a known β-glucosidase inhibitor, was included. 1-O-IAGluc in the presence of castanospermine stimulated growth of corn coleoptiles segments even stronger then free IAA. So, it seems that...
Article
Full-text available
The endogenous content of IAA in the cotyledons of Pharbitis nil is low before and during the first half of the inductive 16-h-long dark period. From the 8th to the 12th hour the level of IAA increased and then again was going down at the end of a dark period. Exogenous IAA applied to the cotyledones before and during the first half of the inductiv...
Article
Full-text available
The study examined the influence of light and auxin on the transcription level of PnACO3, a gene involved in ethylene production, in relation to the inhibitory effect of ethylene on flower induction in the short-day plant Pharbitis nil (=Ipomoea nil). Exogenous auxin was shown to increase the level of PnACO3 mRNA, with the effect depending on the e...
Poster
Plant hormones auxin have been implicated in almost every aspects of plant development. In the past few years, it was shown that TIR1 (TRANSPORT INHIBITOR RESPONSE1) and some close homologs act as auxin receptors in Arabidopsis thaliana. TIR1 is an integral component of the SKP1/CULLIN/F-BOX PROTEIN (SCF)TIR1 complex that mediates the degradation o...
Poster
The formation and development of flowers and seeds of lupines are crucial for the productivity of the plants. This stage of development, however, is associated with undesirable phenomenon, which is the flowers abscission. The physiological studies and field tests show that to the moment the seeds reach full maturity, on plant stay only pods on the...
Poster
Phytohormone auxin is a key player during pattern formation, organogenesis, and various physiological processes. Auxin signaling and plant development depend on a family of partially redundant F-box receptors of the TAAR (TRANSPORT INHIBITOR RESPONSE 1 / AUXIN SIGNALING F-BOX ROTEIN 2 AUXIN RECEPTOR) clade. They act as a part of the ubiquitin ligas...
Article
Full-text available
MicroRNAs regulate gene expression by guiding the cleavage or attenuating the translation of target mRNAs. In Arabidopsis thaliana, the subset of class II TCP genes (plant-specific group of transcription factors) contains an miR319-binding site. One of them, AtTCP4, regulates negatively leaf growth and positively leaf senescence. In addition, miR31...
Article
Full-text available
Abscisic acid is one of the plant hormones that determines normal growth and development, i.e. seeds ripening and germination, stomata opening and closure, flowering and stress responses. An appropriate level of endogenous ABA plays a key role in the regulation of most of these processes. Its content in a particular tissue is a balance between the...
Poster
The plant hormone auxin regulates a broad spectrum of developmental processes. Recent advances have been made in the understanding of auxin response pathways, primarily by the characterization of genes encoding Auxin Response Factors (ARF) in Arabidopsis. In addition, expression of some of ARFs is regulated by microRNAs (miRNAs). In A. thaliana, AR...
Conference Paper
The plant hormone auxin regulates a broad spectrum of developmental processes. Recent advances have been made in the understanding of auxin response pathways, primarily by the characterization of genes encoding Auxin Response Factors (ARF) in Arabidopsis. In addition, expression of some of ARFs is regulated by microRNAs (miRNAs). In A. thaliana, AR...
Article
Full-text available
Gibberellins (GA), as one of the most important phytohormones, control different aspect of plant growth and development such as seed germination, stem elongation and floral induction. Although identified more than a hundred and thirty GA, only a small number of them are biological active. Many non-bioactive GA are present in plant tissues as precur...
Article
Full-text available
Jasmonates are plant hormones involved in many growth and development processes. They also participate in plant defense responses. Current progress in the study on biosynthesis and signaling of jasmonates has contributed to the understanding of the mechanisms regulating concentration of these hormones in the cell. Sustaining a proper level of jasmo...
Article
Signaling pathways, and specifically the signaling pathway of calcium, have been widely implicated in the regulation of a variety of signals in plants. Calcium-dependent protein kinases (CDPKs) are essential sensor-transducers of calcium signaling pathways, the functional characterization of which is of great interest because they play important ro...
Article
Little work was done so far with phytochrome from Pharbitis nil. Purification of phyA from this plant has been exceptionally difficult. Labile phytochrome was presented in too small amount to obtain either absorption spectra or enough protein to produce antibodies. Monoclonal antibodies mAP5, MAC 50, 52, 198 recognized Pharbitis nil labile phytochr...
Article
Full-text available
In this study, the first ACC oxidase (PnACO1) cDNA from model short-day plant Pharbitis nil was isolated. The expression pattern of PnACO1 was studied under different conditions (photoperiod and auxin), an adequate balance of which determines P. nil flowering. It was shown that the gene was transcribed in all the examined organs of the 5-day-old se...
Article
Streszczenie: Gibereliny (GA), jako jedne z podstawowych fitohormonów, kontrolują tak ważne procesy wzrostu i rozwoju roślin jak kiełkowanie nasion, wydłużanie łodyg i indukcję kwitnienia. Z ponad stu trzydziestu różnych GA zidentyfikowanych u roślin, grzybów i bakterii tylko nieliczne – GA1, GA3, GA4, GA5, GA6, GA7 – wykazują aktywność biologiczną...
Article
Gibberellins (GAs), as one of the most important phytohormones, control different aspect of plant growth and development including seed germination, stem elongation and flower induction. Among more than a hundred and thirty GAs identified from plants, fungi and bacteria only a small number of them, such as GA(1), GA(3), GA(4), GA(5), GA(6) and GA(7...
Article
Full-text available
Application of gibberellic acid (GA3) on the cotyledons of 5-d-old Pharbitis nil reversed the inhibitory effect of both abscisic acid (ABA) and ethylene on flowering. Application of GA3 slightly decreased ethylene production and did not affect the endogenous ABA content in the cotyledons during the night. However, it reversed the stimulating effect...
Article
Full-text available
In this study using biochemical approaches we identified two calcium-dependent protein kinases (CDPKs) named PnCDPK52 and PnCDPK56 in soluble protein extracts from seedlings of Pharbitis nil. Both enzymes phosphorylated the specific substrate histone III-S in the presence of Ca2+ and cross-reacted with antibodies against the CDPK. PnCDPKs exhibited...
Article
Full-text available
Interactions between methyl jasmonate (JA-Me) and ethylene in the photoperiodic flower induction of short-day plant Pharbitis nil were investigated. Both JA-Me and gaseous ethylene applied during the inductive long night caused a decrease in the number of flower buds generated by P. nil. Application of ethylene did not affected niether the level of...
Article
Full-text available
Flowering of plants is controlled by hormones among which both stimulators and inhibitors are present. The role of abscisic acid (ABA) in flower induction of the short day plant Pharbitis nil was shown in our experiments through exogenous applications and endogenous level determination of the hormone in cotyledons of seedlings grown under special l...
Article
Precise control of flowering time and generative organs development is essential for successful plants reproduction. Recent years showed that microRNA is one of the factors involved in regulation of generative development in plants. MicroRNA are 21 bp length regulatory molecules. They are involved in target genes silencing by inactivating they expr...
Article
Full-text available
Precise control of flowering time and generative organs development is essential for successful plants reproduction. Recent years showed that microRNA is one of the factors involved in regulation of generative development in plants. MicroRNA are 21 bp length regulatory molecules. They are involved in target genes silencing by inactivating they expr...
Article
Calcium-dependent protein kinases (CDPKs) are central calcium signal decoders. Here we report the isolation and characterization of the Japanese Morning Glory’s (Pharbitis nil) CDPK gene, termed PnCDPK1. The full-length cDNA of 1943bp contains an open reading frame for PnCDPK1 consisting of 514 amino acid residues and a calculated molecular mass of...
Article
Full-text available
In this work we studied interactions between auxin and ethylene in the inhibition of photoperiodic flower induction in SDP Pharbitis nil. To this end, PnACS2 was cloned and its expression was studied under various photoperiodic conditions, as well as under the influence of auxin. The full-length PnACS2 cDNA consists of 1,841bp and encodes for 472 a...
Article
Guanylyl cyclases (GCs) are enzymes involved in the biosynthesis of cyclic guanosine monophosphate (cGMP). Here we report the cloning and characterization of a new guanylyl cyclase, designated PnGC-1, from Pharbitis nil. This gene encodes a protein of 286 amino acids, with a calculated molecular mass of 32kDa. The predicted amino acid sequence cont...