Jan Jezbera- Austrian Academy of Sciences (OeAW)
Jan Jezbera
- Austrian Academy of Sciences (OeAW)
About
131
Publications
5,637
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,625
Citations
Current institution
Publications
Publications (131)
We studied the diversity of Limnohabitans using Reverse Line Blot Hybridization with Limnohabitans lineage-specific probes in the freshwater canyon-shaped Římov Reservoir (CZ). To examine the succession of distinct lineages we performed two studies: (i) an intensive spring sampling program at the lacustrine part of the Římov Reservoir (from ice mel...
Bacteria of the genus Limnohabitans, more precisely the R-BT lineage, have a prominent role in freshwater bacterioplankton communities due to their high rates of substrate uptake and growth, growth on algal-derived substrates and high mortality rates from bacterivory. Moreover, due to their generally larger mean cell volume, compared to typical bac...
Different bacterial strains can have different value as food for heterotrophic nanoflagellates (HNF), thus modulating HNF growth and community composition. We examined the influence of prey food quality using four Limnohabitans strains, one Polynucleobacter strain and one freshwater actinobacterial strain on growth (growth rate, length of lag phase...
Different bacterial strains can have different value as food for heterotrophic nanoflagellates (HNF), thus modulating HNF growth and community composition. We examined the influence of prey food quality using four Limnohabitans strains, one Polynucleobacter strain and one freshwater actinobacterial strain on growth (growth rate, length of lag phase...
Actinorhodopsins (ActRs) are recently discovered proteorhodopsins present in Actinobacteria, enabling them to adapt to a wider spectrum of environmental conditions. Frequently, a large fraction of freshwater bacterioplankton belongs to the acI lineage of Actinobacteria and codes the LG1 type of ActRs. In this paper we studied the genotype variabili...
Among abundant freshwater Betaproteobacteria, only few groups are considered to be of central ecological importance. One of them is the well-studied genus Limnohabitans and mainly its R-BT subcluster, investigated previously mainly by fluorescence in situ hybridization methods. We designed, based on sequences from a large Limnohabitans culture coll...
RLBH detections of probe-defined groups scored as no (no color), weak (light grey color), normal (dark grey color) and strong (black color) across all 161 habitats. For more parameters of habitats see Jezberová et al., 2010; Šimek et al., 2010; Jezbera et al., 2011 and 2012.
(DOC)
Table S1. Pairwise comparisons of aligned almost complete 16S rRNA gene sequences of newly isolated Limnohabitans strains and closely related environmental clones and other genera. The similarity is shown in the upper part, the lower part depicts the number of nucleotide differences between sequences. Newly described strains are in bold. Environmen...
The distribution and abundance of Betaproteobacteria and three of its genera - Limnohabitans (R-BT065 lineage), Polynucleobacter (including subclusters Polynucleobacter necessarius and Polynucleobacter acidiphobus/Polynucleobacter difficilis), and Methylophilus - across the epilimnia of 72 limnologically diverse freshwater habitats were investigate...
Background:
The bacterial taxon Polynucleobacter necessarius subspecies asymbioticus represents a group of planktonic freshwater bacteria with cosmopolitan and ubiquitous distribution in standing freshwater habitats. These bacteria comprise <1% to 70% (on average about 20%) of total bacterioplankton cells in various freshwater habitats. The ubiqui...
Lack of genes well known from other Burkholderiaceae bacteria.
(DOCX)
Loci used for the multilocus sequence analysis of the eleven F10 lineage strains isolated from Pond-1 (compare Fig. 5). The sequences of Pnuc_1240 were not considered for the calculation of the tree shown in Fig. 5.
(DOCX)
Experiment on growth support of Polynucleobacter bacteria by algal exudates.
(DOCX)
Bootstrap values obtained in phylogenetic reconstructions using the concatenated amino acid (AA) or nucleotide sequences (nt) of eight housekeeping genes (Fig. 8). For comparison, phylogenetic reconstructions solely based on the 16S rRNA gene sequences of the same set of organisms were performed (tree not shown). Note the strong support for node E...
Current taxonomy of Polynucleobacter bacteria. The genus Polynucleobacter currently harbors five species (Hahn et al., 2009, 2010, 2011a, 2011b, 2012). These five species represent the four Polynucleobacter tribes (PnecA to PnecD) suggested by the taxonomy in Newton et al. (2011). Some of the strains affiliated with the subspecies P. necessarius as...
Results of the assimilation experiments performed with 93 substrates. Substrates that did not support growth of the strain are highlighted in red. Substrates yielding growth efficiencies ≥5% are highlighted in green. Growth efficiencies were not determined for five of the listed substrates.
(DOCX)
Loci used for the multilocus sequence analysis shown in Fig. 8.
(DOCX)
Genomes and strains considered for the multilocus sequence analysis presented in Fig. 8.
(DOCX)
Search for genes involved in adaptations to a planktonic lifestyle.
(DOCX)
The subspecies Polynucleobacter necessarius asymbioticus (> 99% 16S rRNA similarity) has a cosmopolitan distribution and a ubiquitous occurrence in lentic freshwater habitats. We tested if the observed ubiquity of these free-living planktonic freshwater bacteria results from a euryoecious (generalist) adaptation of P. n. asymbioticus strains, or fr...
Two bacterial strains, II-B4(T) and II-D5(T), isolated from the meso-eutrophic freshwater Římov reservoir (Czech Republic), were characterized phenotypically, phylogenetically and chemotaxonomically. Both strains were chemo-organotrophic, facultatively anaerobic, non-motile rods, with identical DNA G+C contents of 59.9 mol%. Their major pola...
A chemo-organotrophic, aerobic, non-motile strain, MWH-BRAZ-DAM2D(T), isolated from a freshwater pond in Brazil, was characterized phenotypically, phylogenetically and chemotaxonomically. Phylogenetic analysis of 16S rRNA gene sequences indicated affiliation of the strain with the genus Limnohabitans (Comamonadaceae, Betaproteobacteria). 16S rRNA g...
The distribution of the phylogenetically narrow R-BT065 cluster (Betaproteobacteria) in 102 freshwater lakes,
reservoirs, and various ponds located in central Europe (a total of 122 samples) was examined by using a
cluster-specific fluorescence in situ hybridization probe. These habitats differ markedly in pH, conductivity,
trophic status, surface...
The distribution of the phylogenetically narrow R-BT065 cluster (Betaproteobacteria) in 102 freshwater lakes, reservoirs, and various ponds located in central Europe (a total of 122 samples) was examined by using a cluster-specific fluorescence in situ hybridization probe. These habitats differ markedly in pH, conductivity, trophic status, surface...
We present a survey on the distribution and habitat range of Polynucleobacter necessarius ssp. asymbioticus (PnecC), a numerically and functionally important taxon in the plankton of freshwater systems. We systematically sampled stagnant freshwater habitats in a heterogeneous 2000 km(2) area, together with ecologically different habitats outside th...
A chemo-organotrophic, aerobic, facultatively anaerobic, non-motile strain, MWH-C5(T), isolated from the water column of the oligomesotrophic Lake Mondsee (Austria), was characterized phenotypically, phylogenetically and chemotaxonomically. The predominant fatty acids of the strain were C(16 : 1)omega7c/omega6c, C(16 : 0), C(12 : 1) and C(8 : 0)-3O...
Actinobacteria often constitute a large fraction of the bacterioplankton in freshwater systems. Cultivation-independent methods have revealed that the so-called acI lineage frequently represents the most numerous taxon among assemblages of freshwater Actinobacteria and even among total freshwater bacterioplankton. Bacteria affiliated with this uncu...
Availability of phosphate for phytoplankton and bacteria and of glucose for bacteria was studied in mesocosms using NW Mediterranean coastal water with added phosphate, glucose, or a combination of both. We observed an initial increase of bacterial production in all mesocosms, a con- tinuous increase of particulate P only in the P-enriched mesocosm...
We compared the influences of grazing by the bacterivorous nanoflagellate Poterioochromonas sp. strain DS on ultramicrobacterial Actinobacteria affiliated with the Luna-2 cluster and ultramicrobacterial Betaproteobacteria of the species Polynucleobacter cosmopolitanus. These bacteria were almost identical in size (<0.1 μm3) and shape. Predation on...
Microbial rhodopsins are membrane proteins that utilize a retinal chromophore to harvest sunlight for energetic and photosensory functions. Recently, a group of novel rhodopsin sequences named 'actinorhodopsins' (ActRs) was hypothesized to exist among uncultured planktonic Actinobacteria. ActRs were discovered by mining metagenomic data obtained du...
