Jan Gebauer

Jan Gebauer
Veterinary Research Institute, Brno | VUVEL · Immunology

PhD

About

10
Publications
19,774
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33
Citations

Publications

Publications (10)
Article
Economic importance of common carp (Cyprinus carpio L.) increases every year. Viral diseases are major threat for carp aquaculture and cause significant economic losses. Koi herpesvirus (KHV) is one of the most serious carp diseases. Current study is focused on confirmation of possible differences in early immune response to KHV depending on level...
Article
Distinct monocyte subpopulations have been previously described in healthy pigs and pigs experimentally infected with Actinobacillus pleuropneumoniae (APP). The CD163+ subpopulation of bone marrow (BM), peripheral blood (PB) and lung monocytes was found to play an important role in the inflammatory process. The inflammation is accompanied by elevat...
Article
Dendritic cells (DCs) represent a heterogeneous group of major antigen-presenting cells, responding to different stimuli in their microenvironment. They are able to activate naïve T-cells and drive their polarization towards effector types. However, the effect of different Th-polarizing cytokine microenvironment on porcine DCs remains poorly descri...
Article
In Glässer’s disease outbreaks, Glaesserella (Haemophilus) parasuis has to overcome the non-specific immune system in the lower respiratory tract, the alveolar macrophages. Here we showed that porcine alveolar macrophages (PAMs) were able to recognize and phagocyte G. parasuis with strain-to-strain variability despite the presence of the capsule in...
Article
Dirofilaria repens and Dirofilaria immitis are the most common filarial species affecting humans in Europe. Dirofilaria repens causes subcutaneous or ocular infection, whereas D. immitis is responsible mainly for the pulmonary form. In this report, we present the first human case of periorbital dirofilariasis in the Czech Republic. A 58-year-old wo...
Article
Glaesserella (Haemophilus) parasuis is a part of the normal flora of the respiratory tract of pigs. However, under certain conditions it can also induce severe systemic disease with high morbidity and mortality leading to gross economic losses in the pig industry. The most prevalent serovars in pig herds in the Czech Republic are the virulent serov...
Article
Full-text available
Background Salmonella enterica serovar Typhimurium is one of the most common enteropathogenic bacteria found in pigs in Europe. In our previous work, we demonstrated the protective effects in suckling piglets when their dams had been vaccinated with an S. Typhimurium-based inactivated vaccine. This study is focused on a procedure leading to serolog...
Article
Full-text available
Background Lactoferrin (LF) is an 80 kDa glycoprotein which is known for its effects against bacteria, viruses and other pathogens. It also has a high potential in nutrition therapy and welfare of people and a variety of animals, including piglets. The ability to bind lipopolysaccharide (LPS) is one of the described anti-inflammatory mechanisms of...
Article
Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Derby are the most common serovars of Salmonella enterica ssp. enterica found in pigs in Europe. We previously observed that suckling piglets of sows vaccinated with an S. Typhimurium-based inactivated vaccine are protected against homologous strain challenge. To develop this v...

Questions

Questions (4)
Question
How does one build an experiment using TMT-sixplex when you have 6 different conditions (one of them is untreated control, which the others are related to) and 5 biological replicates in each condition (30 samples). Is it possible to pool samples for each group and perform one analysis, or to do 5 different sets of 6-plexes?
What software is needed for processing such types of data you prefer? Thanks for your suggestions.
Question
I was quite disappointed by low specificity of Ni-NTA for binding His-tagged proteins. Many other proteins have affinity to nickel because of the metal-binding motifs in their structure. Any advice on how to increase specificity only for real His-tag (for purification as well as detection)? What about experiences with anti-His-tag antibodies?

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