Jamila Bernardi

Genetic markers can be used in seeds and in plants to distinguish drug-type from fiber-type Cannabis Sativa L. varieties even at early stages, including pre-germination when cannabinoids are not accumulated yet. With this aim, this paper reports sequencing results for tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBD...

Kernel size in cereal is an important agronomic trait controlled by the interaction of genetic and environmental factors. The endosperm occupies most of the kernel area; for this reason, the endosperm cells dimension, number and metabolic content strongly influence kernel properties. This paper presents the transcriptomic and metabolomic analysis o...

Maize is a staple food source in the world, whose ancient varieties or landraces are receiving a growing attention. In this work, two Italian maize cultivars with pigmented kernels and one inbred line were investigated for untargeted phenolic profile, in vitro antioxidant capacity and resistance to Fusarium verticillioides infection. “Rostrato Ross...

Interest in using polyphenols as modulators of the activity of digestive enzymes is rapidly increasing. The main purpose of this study was to investigate the role of phenolic compounds characterising pigmented maize flours in the modulation of in vitro starch digestibility. Flours from three different pigmented maize varieties were evaluated under...

The compounds, significantly different among the three samples (one-way ANOVA, multiple testing correction, p-value 0.05), are reported with the corresponding P-value.

The results of the volcano plot analysis reporting, for each pairwise comparison, the compounds characterized by a fold change higher than 2.

A full list of the 260 phenolic compounds is provided.

Vanilla is a flavoring recovered from the cured beans of the orchid genus Vanilla. Vanilla ×tahitensis is traditionally cultivated on the islands of French Polynesia, where vanilla vines were first introduced during the nineteenth century and, since the 1960s, have been introduced to other Pacific countries such as Papua New Guinea (PNG), cultivate...

Molecular markers are useful tools for measuring the genetic diversity among agricultural species. In plants, microsatellites are still the most used markers for germplasm characterization, conservation, and traceability purposes, while in the livestock sector, although having represented the standard for at least two decades, they are still used o...

Leaves of globe artichoke are food industry by-products gaining interest due to their therapeutic and nutraceutical potential. The total phenolics, flavonoids and flavonols content, as well as radical scavenging capacity and reducing antioxidant power were determined in leaves of nineteen artichoke cultivars. An untargeted analysis based on high-re...

There is a growing interest among consumers and researchers in the globe artichoke [Cynara cardunculus L. subsp. scolymus (L.) Hegi] leaf extract due to its nutraceutical and therapeutic properties. The application of an abiotic stress such as salinity can activate the stress-signaling pathways, thus enhancing the content of valuable phytochemicals...

Principal Component Analysis (PCA) of artichoke phenolic metabolites in KCl stressed and control plants.

Unsupervised hierarchical cluster analysis of artichoke metabolites in KCl stressed and control plants.

Raw data from metabolomic analysis (phenolic compounds in the first sheet and plant metabolites in the second), together with identification scores and composite spectrum.

The dek18 mutant of maize was previously classified as a collapsed kernel mutant named cp*-931A, which has a decreased auxin content in kernels. Molecular and functional characterization of this mutant line offers the possibility to better understand auxin biology during maize seed development. Seeds of the dek18 mutants are smaller compared to wil...

The seed represents the unit of reproduction of flowering plants, capable of developing into another plant, and to ensure the survival of the species under unfavorable environmental conditions. It is composed of three compartments: seed coat, endosperm and embryo. Proper seed development depends on the coordination of the processes that lead to see...

Bsister proteins form a clade of MADS-box transcription factors that originated 300 million years ago, after ferns diverged but before Angiosperms and Gymnosperms lineages did. Thus, Bsister proteins have been found in both Gymnosperm and Angiosperm species such as paddy oat (Gnetum gnemon), ginkgo, yew (Taxus baccata), rape seed, rice, maize, whea...

Citrus fruits are an important nutritional source for human health and have immense economic value. Fruit development and ripening are key processes in the production of the phytonutrients, which are essential for a balanced diet and for disease prevention. The anthocyanins are responsible for red pigmentation in the flesh of sweet orange and one o...

Flavonoids are the most common antioxidant compounds produced in plants. In this study, two wild types and two independent mutants of Medicago truncatula with altered anthocyanin content in leaves were characterized at the phenotype, metabolite profile, gene structure and transcript levels. Flavonoid profiles showed conserved levels of dihydroflavo...

Sweet orange [Citrus sinensis (L.) Osbeck] represents the most important Citrus species, followed by clementine (C. clementina Hort. ex Tan.). Citrus species and genotypes are difficult to recognize as they have a moderate level of diversity due to nucellar selection, vegetative propagation and origin by single spontaneous mutation. Despite the lar...

The phytohormone auxin (IAA) plays a fundamental role in vegetative and reproductive plant development. Here, we characterized a seed-specific viable maize mutant, defective endosperm18 (de18) that is impaired in IAA biosynthesis. de18 endosperm showed large reductions of free IAA levels and is known to have ~40% less dry mass, compared with De18....

Fusarium ear rot is one of the most important diseases of maize, that is of concern because Fusarium verticillioides produces the mycotoxins known as fumonisins. F. verticillioides can be transmitted either through infected silks or seed-to-kernel. In order to better understand the virulence of F. verticillioides, the effect of the fungus on the de...

A flesh-specific oligonucleotide custom array was designed to study gene expression during blood orange ripening. The array included 301 probes derived from a subtracted SSH library, a cDNA-AFLP collection, and a set of regulatory genes from the HarvEST Citrus database. The custom array was hybridized using fruits of 'Moro', a blood cultivar, and '...

A flesh-specific oligonucleotide custom array was designed to study gene expression during blood orange ripening. The array included 301 probes derived from a subtracted SSH library, a cDNA-AFLP collection, and a set of regulatory genes from the Harvest citrus database. The custom array was hybridized using samples of Moro, a pigmented cultivar, an...

Protocols for efficient co-transformation of potato internodes with genes contained in separate plasmids or gene cassettes (i.e., linear PCR fragments comprising a promoter-gene-terminator) using particle bombardment were established. Twenty-eight out of 62 (45%) and 11 out of 65 (17%) plants transformed with a plasmid containing the selectable mar...

We have tested amplified fragment length polymorphism (AFLP) technology, in comparison with isoenzyme analysis, for the simultaneous detection of inter- and intraspecific cell line cross-contaminations (CCCs) in the cell line collection held at the Istituto Zooprofilattico della Lombardia e dell'Emilia Romagna. Isoenzyme analysis identified four ca...

As in human research, also in livestock species the use of continuous cell cultures is an important tool for the study of physiological and tissue developmental processes, as well as for immunological, virological and toxicological assays. This widespread use of animal cell cultures needs that quality control tests are systematically performed in o...

Reliable methods for tracing animal products to breeds or their region of origin would allow a certification of regional products. This would contribute to consumers confidence and to the exploitation of local genetic resources in marginal rural areas. Different methods can be envisaged to assign individuals to breeds or populations exploiting mole...

Sweet orange [Citrus sinensis (L.) Osbeck] represents the most important Citrus species cropped in the world followed by Clementine (C. clementina Hort. ex Tan.) in Mediterranean areas. Citrus species and genotypes are difficult to recognize as they have moderate level of diversity due to nucellar selection, vegetative propagation and the origin by...