Jamie Bojko

Jamie Bojko
Teesside University · Department of Science and Environment

B.Sc. Genetics (IND), Ph.D.
Disease Ecologist exploring the impact of symbiotic organisms on biological invasion dynamics and native animal health.

About

68
Publications
24,812
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
716
Citations
Citations since 2017
63 Research Items
700 Citations
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
2017201820192020202120222023050100150200250
Introduction
My research involves screening for and systematically identifying the symbionts of invertebrate hosts; primarily including Crustacea and Mollusca. I work mainly with Microsporidia, bacteria and viruses, and use histological, TEM, molecular diagnostic and metagenomic methods to better understand the microbiomes of invertebrate hosts. Follow me on Twitter: @Jamiebojko
Additional affiliations
December 2019 - present
Teesside University
Position
  • Lecturer
October 2017 - October 2019
University of Florida
Position
  • Researcher
September 2013 - December 2016
University of Leeds
Position
  • PhD Student
Education
September 2009 - July 2013
University of Leeds
Field of study
  • Genetics

Publications

Publications (68)
Article
Full-text available
Background: Mangrove forests are productive ecosystems, acting as a sink for CO2, a habitat for a diverse array of terrestrial and marine species, and as a natural barrier to coastline erosion. The species that reside within mangrove ecosystems have important roles to play, including litter decomposition and the recycling of nutrients. Crustacea a...
Article
Full-text available
Microsporidian diversity is vast. There is a renewed drive to understand how microsporidian pathological, genomic, and ecological traits relate to their phylogeny. We comprehensively sample and phylogenetically analyse 125 microsporidian genera for which sequence data are available. Comparing these results with existing phylogenomic analyses, we su...
Article
Full-text available
A microsporidian parasite infecting the pheasantshell mussel, Actinonaiais pectorosa, was discovered in a freshwater system in the USA. The original description of this species placed it into the holding genus “Microsporidium”; however, the availability of ecological, environmental, histological, electron microscopy, and genetic data, suffice to pr...
Article
Full-text available
Callinectes sapidus, or the ‘blue crab’, supports an extensive east-coast USA fishery and was one of the first crustacean species in which viruses were observed. Pioneering research by Dr Phyllis Johnson led to these initial discoveries, one of which included the discovery of a virus termed “Baculovirus-A”. This virus was considered a potential mem...
Article
Full-text available
The microsporidian diversity catalogued so far has resulted in the development of several taxonomic groups, one of which is the Enterocytozoonida – a group of generalist ‘ultimate opportunists’, which infect many fished and aquacultured animals, as well as a broad suite of host taxa, including humans. In this study, we provide phylogenetic, ultrast...
Article
Many marine habitats are at risk due to increasing frequency, intensity, and persistence of harmful algal blooms. Repeated cyanobacterial armful algal blooms (cyanoHABs) in Florida Bay, USA, kill sponges, resulting in reduced filtration and loss of shelter for benthic species. The loss of these key ecosystem functions can impact disease dynamics if...
Article
Full-text available
Pollution, biological invasions and climate change pose the greatest threats to biodiversity worldwide. Few studies explore the interlink between invasive species and local pollution sites. This study describes the type and cytochemical properties of haemocytes from invasive Carcinus maenas on the southwestern Atlantic coast (Argentina). Moreover,...
Chapter
Around 57.1% of microsporidia occupy aquatic environments, excluding a further 25.7% that utilise both terrestrial and aquatic systems. The aquatic microsporidia therefore compose the most diverse elements of the Microsporidia phylum, boasting unique structural features, variable transmission pathways, and significant ecological influence. From dee...
Article
Ovipleistophora (Microsporidia) is a globally distributed genus of obligate parasites that infect fish, Crustacea, and trematodes. We report on two additional crayfish hosts, Procambarus pictus (Simms Creek, Florida) and Procambarus fallax (Santa Fe River, Florida), that exhibited signs of high-intensity microsporidian infection in the musculature....
Article
Full-text available
Crayfish are common hosts of microsporidian parasites, prominently from the genus Thelohania. Thelohania is polyphyletic, with multiple genetically distinct lineages found from freshwater and marine environments. Researchers have been calling for a revision of this group for over a decade. We provide evidence that crayfish-infecting, freshwater The...
Research Proposal
Full-text available
We propose the generation of a new species within the Gammanudivirus genus to hold ‘Dikerogammarus haemobaphes nudivirus’ (Allain et al. 2020) – Gammanudivirus dikhaemobaphedis. The species name is based on the name of the host Dikerogammarus haemobaphes (Amphipoda). The genome of the virus is a circular molecule consisting of 119,695bp and encodes...
Article
Full-text available
Bacterial pathogens are a long-standing threat to the longevity and survival of crustacean hosts. Their presence and continuing emergence require close monitoring to understand their impact on fished, cultured, and wild crustacean populations. We report the presence of a new bacterial pathogen belonging to the Anaplasmataceae (Alphaproteobacteria:...
Article
Invasive non-native amphipods (Crustacea) are becoming a model system in which to explore the impact and diversity of invasive parasites - parasites that are carried along an invasion route with their hosts. Gammarus varsoviensis is a freshwater amphipod species that has a recently explored invasion history. We provide a histopathological survey fo...
Article
Full-text available
Biological invasions are a driving force for biodiversity decline, worldwide. These dynamic systems often include the transference of symbiotic or pathogenic organisms that display their own threat to local fauna. Alternatively, parasites introduced during an invasion can help to control the invasive host population and limit ecological damage. To...
Article
Full-text available
In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, a...
Article
Full-text available
This study provides a broad phylogenetic analysis for the Eubrachyura, with the inclusion of three new Panopeidae mitochondrial genomes: Eurypanopeus depressus (flatback mud crab) (15,854bp), Panopeus herbstii (Atlantic mud crab) (15,812bp) and Rhithropanopeus harrisii (Harris, or ‘white-fingered’ mud crab) (15,892bp). These new mitogenomes were an...
Article
Full-text available
Invasive Non-Native Species (INNS) can co-transport externally and internally other organisms including viruses, bacteria and other eukaryotes (including metazoan parasites), collectively referred to as the symbiome. These symbiotic organisms include pathogens, a small minority of which are subject to surveillance and regulatory control, but most o...
Article
Full-text available
Invasive Non-Native Species (INNS) can co-transport externally and internally other organisms including viruses, bacteria and other eukaryotes (including metazoan parasites), collectively referred to as the symbiome. These symbiotic organisms include pathogens, a small minority of which are subject to surveillance and regulatory control, but most o...
Article
Full-text available
The Ponto-Caspian region is the main donor of invasive amphipods to freshwater ecosystems, with at least 13 species successfully established in European inland waters. Dikerogammarus spp. and Pontogammarus robustoides are among the most successful, due to their strong invasive impact on local biota. However, genomic knowledge about these invaders i...
Article
In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, a...
Article
Full-text available
Multiple enveloped viruses with rod-shaped nucleocapsids have been described, infecting the epithelial cell nuclei within the hepatopancreas tubules of crustaceans. These bacilliform viruses share the ultrastructural characteristics of nudiviruses, a specific clade of viruses infecting arthropods. Using histology, electron microscopy and high throu...
Article
Say’s mud crab, Dyspanopeus sayi (Brachyura: Panopeidae) is a native shallow subtidal and inter-tidal inhabitant of the Atlantic coastline of North America and an invasive species in the Mediterranean and Black Seas. Little is known about the microparasites of this host and the broader Panopeidae. We describe a novel microsporidian parasite infecti...
Article
Biological invasions have the capacity to introduce non-native parasites. This study aimed to determine whether the invasive green crab population, Carcinus spp., on the Southwestern Atlantic coast of Argentina harbours any symbionts, and whether these may spillover or spillback between native crabs, Cyrtograpsus altimanus and C. angulatus. Macrosc...
Preprint
Invasive Non-Native Species (INNS) can co-transport externally and internally other organisms including viruses, bacteria and other eukaryotes (including metazoan parasites), collectively referred to as the symbiome. These symbiotic organisms include pathogens, a small minority of which are subject to surveillance and regulatory control, but most o...
Article
Full-text available
Aquatic invasions are a major ecological and socioeconomic concern. Management of invasive aquatic populations requires a robust understanding of the effectiveness and suitability of control methods. In this review, we consider multiple control options for the management of invasive aquatic amphipods, exploring their efficacy and application constr...
Article
Invasive crayfish and the introduction of non-native diseases pose a significant risk for the conservation of endangered, white-clawed crayfish (Austropotamobius pallipes). Continued pollution of waterways is also of concern for native species and may be linked with crayfish disease dynamics. We explore whether crayfish species or environmental qua...
Article
A positive, single-stranded RNA virus is identified from the transcriptome of Probopyrinella latreuticola Gissler, 1882; a bopyrid isopod parasite of the Sargassum shrimp, Latreutes fucorum Fabricius, 1789. The viral sequence is 13,098 bp in length (including polyA), encoding four open reading frames (ORF). ORF-1 encodes a polyprotein, with three c...
Article
Invasive non-native species (INNS) pose a risk as vectors of parasitic organisms (Invasive Parasites). Introducing invasive parasites can result in ecological disturbances, leading to biodiversity loss and native species illness/mortality, but occasionally can control INNS limiting their impact. Risks to human health and the economy are also associ...
Article
Full-text available
The Nudiviridae are a family of large double-stranded DNA viruses that infect the cells of the gut in invertebrates, including insects and crustaceans. The phylogenetic range of the family has recently been enhanced via the description of viruses infecting penaeid shrimp, crangonid shrimp, homarid lobsters and portunid crabs. Here we extend this by...
Article
Cirolana westbyi n. sp., a novel species in the ‘Cirolana parva-group’ is described from the Turneffe Atoll, Belize using an integrated molecular and morphological approach. The 18S ribosomal RNA gene for C. westbyi n. sp. and also for Cirolana parva Hansen, 1890 are provided, in addition to new primers, developed for the Cirolanidae 18S gene. The...
Article
Full-text available
Among the many Callinectes spp. across the western Atlantic, the blue crab C. sa pidus has the broadest latitudinal distribution, encompassing both tropical and temperate climates.Its life history varies latitudinally, from extended overwintering at high latitudes to year-round activity in tropical locations. Callinectes sapidus reovirus 1 (CsRV1)...
Article
Full-text available
The blue crab Callinectes sapidus is one of the most widely studied marine crustaceans due to its high economic value and ecological significance. Despite extensive research on the blue crab in North America, many questions remain about the distribution and abundance of the species in the subtropics and tropics. In many places, C. sapidus is sympat...
Article
Crayfish are a vital ecological asset in their native range but can be highly damaging as invasive species. Knowledge of their diseases, including high levels of research on Aphanomyces astaci (crayfish plague), show that disease plays a vital role during crayfish invasions. Microsporidian diseases in crayfish are less studied but are thought to ha...
Article
Full-text available
Recent genomic and metagenomic studies have led to a dramatic expansion of the known diversity of nucleocytoplasmic large DNA viruses (NCLDVs) of eukaryotes, which include giant viruses of protists and important pathogens of vertebrates, such as poxviruses. However, the characterization of viruses from nonmodel hosts still lags behind. We sequenced...
Article
Full-text available
The amphipod Dikerogammarus haemobaphes is a high-risk carrier of parasites that impact wildlife in its non-native range. Studies using the mitochondrial genes, Cytochrome Oxidase Sub-Unit 1 (cox1) and small-subunit ribosomal RNA gene (16S), provide some nucleotide detail for understanding the evolution and phylogeography of this species. Despite t...
Article
Ovipleistophora diplostomuri (Microsporidia) is an obligate parasite of fish and trematodes in the US. In April 2019, an individual crayfish, Procambarus bivittatus (Escambia River, Florida), with a high-intensity microsporidian infection was delivered to the Emerging Pathogens Institute. Histological analysis determined that infection was restrict...
Article
Full-text available
A single-stranded DNA (ssDNA) virus is presented from a metagenomic data set derived from Alphaproteobacteria-infected hepatopancreatic tissues of the crab Eurypanopeus depressus. The circular virus genome (4,768 bp) encodes 14 hypothetical proteins, some similar to other bacteriophages (Microviridae). Based on its relatedness to other Microviridae...
Article
The Trematoda are a group of phylogenetically diverse metazoan parasites that exhibit complex life cycles that often pass through invertebrate and vertebrate hosts. Some trematodes influence their host's behaviour to benefit transmission. Their parasitic influence may impact host population size by inhibiting an individual's reproductive capacity....
Article
Full-text available
This study provides morphological, ultrastructural and phylogenetic characterization of 2 novel species of Haplosporidia (Haplosporidium echinogammari n. sp. and H. orchestiae n. sp.) infecting amphipods of the genera Echinogammarus and Orchestia collected in southwestern England. Both parasites infect the connective tissues associated with the dig...
Article
Full-text available
Carcinus maenas is in the top 100 globally invasive species and harbours a wide diversity of pathogens, including viruses. We provide a detailed description for a novel bunyavirus (Carcinus maenas Portunibunyavirus 1) infecting C. maenas from its native range in the Faroe Islands. The virus genome is tripartite, including large (L) (6766bp), medium...
Article
Full-text available
Amphipods are a group of globally abundant Crustacea present throughout terrestrial, marine and freshwater ecosystems. These organisms host a highly diverse systematic assemblage of parasites and pathogens, which are closely linked to the host’s evolution and ecological niche. Such symbioses have been found to affect the behaviour, physiology and o...
Article
The Trematoda are a group of phylogenetically diverse metazoan parasites that exhibit complex life cycles that often pass through invertebrate and vertebrate hosts. Some trematodes influence their host’s behaviour to benefit transmission. Their parasitic influence may impact host population size by inhibiting an individual’s reproductive capacity....
Chapter
Humans pollute the marine environment biologically, chemically, and physically, which can potentially drive or facilitate the emergence, proliferation, or impact of disease. Here, we synthesize what is known about the effect of biological (e.g., wastewater), chemical (e.g. pharmaceuticals), and physical (e.g., sound/light) pollution on marine disea...
Article
Fluctuating asymmetry (FA), defined as random deviations from perfect bilateral symmetry, is assumed to reflect developmental instability. FA is predicted to increase in response to environmental stress, including parasite infection. In addition, based on theory we predict a higher FA in sexually selected traits, due to their greater sensitivity to...
Article
Crustaceans suffer from diseases that can alter their survival and ecology with additional economic consequences for fisheries and aquaculture. Many parasites have been described from crustaceans and with the advent of novel technologies such as next generation sequencing, the discovery of novel parasites has become increasingly efficient. Molecula...
Article
With over 10,000 species of amphipod currently described, this order is one of the most diverse groups of freshwater and marine Crustacea. Members of this group are globally present, and many are keystone species and ecosystem engineers within their respective ecologies. As with most organisms, disease is a key factor that can alter population size...
Preprint
Full-text available
The Caribbean spiny lobster Panulirus argus is susceptible to infection by Panulirus argus Virus 1 (PaV1), the only virus known to naturally infect any lobster species. However, P. argus is able to mitigate PaV1 transmission risk by avoiding infected individuals. White Spot Syndrome Virus (WSSV) has a particularly wide host range. WSSV has not been...
Article
The Caribbean spiny lobster Panulirus argus is susceptible to infection by Panulirus argus Virus 1 (PaV1), the only virus known to naturally infect any lobster species. However, P. argus is able to mitigate PaV1 transmission risk by avoiding infected individuals. P. argus may also be susceptible to another lethal virus, White Spot Syndrome Virus (W...
Article
Invasive and non-native species can pose risks to vulnerable ecosystems by co-introducing bacterial pathogens. Alternatively, co-introduced bacterial pathogens may regulate invasive population size and invasive traits. We describe a novel candidate genus and species of bacteria (‘Candidatus Aquirickettsiella gammari’) found to infect Gammarus fossa...
Article
Full-text available
The green crab, Carcinus maenas, is an invader on the Atlantic coast of Canada and the USA. In these locations, crab populations have facilitated the development of a legal fishery in which C. maenas is caught and sold, mainly for use as bait to capture economically important crustaceans, such as American lobster (Homarus americanus). The paucity o...
Article
Parasites, including macroparasites, protists, fungi, bacteria and viruses, can impose a heavy burden upon host animals. However, hosts are not without defences. One aspect of host defence, behavioural avoidance, has been studied in the terrestrial realm for over 50 years, but was first reported from the aquatic environment approximately 20 years a...
Article
Full-text available
ABSTRACT: Dikerogammarus haemobaphes is a non-native amphipod in UK freshwaters. Studies identify this species as a low-impact invader in the UK, relative to its cousin Dikerogammarus villosus. It has been suggested that regulation by symbionts (such as Microsporidia) could explain this difference of impact. The effect of parasitism upon D. haemoba...
Thesis
Full-text available
This is a full text by request for my thesis. Please see published papers for citable information. Invasive species are one of the foremost damaging environmental problems for biodiversity and conservation, and can affect human health and man-made structures. They pose a great challenge for pest management, with little known about their control an...
Article
Full-text available
According to the Convention on Biological Diversity, by 2020 invasive alien species (IAS) should be identified and their impacts assessed, so that species can be prioritized for implementation of appropriate control strategies and measures put in place to manage invasion pathways. For one quarter of the IAS listed as the “100 of the world's worst”...
Article
Full-text available
Background: Whilst vastly understudied, pathogens of non-native species (NNS) are increasingly recognised as important threats to native wildlife. This study builds upon recent recommendations for improved screening for pathogens in NNS by focussing on populations of Gammarus roeselii in Chojna, north-western Poland. At this location, and in other...
Article
Parahepatospora carcini n. gen. n. sp., is a novel microsporidian parasite discovered infecting the cytoplasm of epithelial cells of the hepatopancreas of a single Carcinus maenas specimen. The crab was sampled from within its invasive range in Atlantic Canada (Nova Scotia). Histopathology and transmission electron microscopy were used to show the...
Article
Full-text available
Littorina saxatilis is a common intertidal gastropod on shores of the North Atlantic, and an important study species for evolutionary investigations. Its congener L. arcana is much less widely distributed, but both species are common at Old Peak, Yorkshire, UK. The parasite profiles of L. saxatilis and L. arcana from this shore were determined hist...
Article
Full-text available
Dikerogammarus haemobaphes, the 'demon shrimp', is an amphipod native to the Ponto-Caspian region. This species invaded the UK in 2012 and has become widely established. Dikerogammarus haemobaphes has the potential to introduce non-native pathogens into the UK, creating a potential threat to native fauna. This study describes a novel species of mic...
Article
Full-text available
Dikerogammarus villosus, an invasive amphipod, has recently been detected in UK freshwaters. To assess the potential for pathogen introduction with the invader, a year-long histopathology survey of the D. villosus population inhabiting the initial site of detection (Grafham Water, Cambridgeshire, UK) was conducted. Additional samples were collected...
Data
Full-text available
Thelohania contejeani is a microsporidian parasite causing porcelain disease in British freshwater crayfish. This parasite poses a current threat to the endangered white claw crayfish; Austropotamobius pallipes. The transmission cycle of this parasite has not been fully described. The transmission of T. contejeani has been previously shown to occur...

Questions

Questions (15)
Question
Hey everyone,
I was wondering if anyone has tested/published some crustacean focussed 18S primers that provide an amplicon size of around 1kb to use for phylogenetics?
Many thanks!
Question
I need to download BioLinux but down want to wipe my laptop. I have been told I can download onto a USB or external hard drive but I cant find a definitive method that walks me through the process.
Any help is great,
Jamie
Question
Hi,
I am currently looking into the development of Bt toxins for crustacean control and have come across zero articles that attempt to develop Bt toxins that target, and induce mortality in, Crustacea. 
Some information is available around Crustacea as non-targets for Bt toxin developed specified for insect control but I am specifically interested in this toxin being targeted towards Crustacea.
Does anyone know of any literature or company activities that could be developing Bt toxins that specifically target Crustacean pests?
Thank you,
Jamie
Question
Hi everyone,
Today I put on a MiSeq run using Nextera XT library preparation to be run on a V3 600 cartridge. After ~20 cycles it has given me a low cluster density of 473K/mm2. The quality however is fantastic (98.9% over 30).
The sample consisted of 6 uniquely barcoded libraries that were equalised in 24ul before adding to PhiX 6ul and HT1. I checked all samples using QF and my individual concentrations of DNA were between 0.6 and 3.5 ng/ul. is this a little low and part of the issue?
Does anyone know why this low cluster density may have come about so I can trouble shoot this in the future? So far I have looked into the NaOH concentration and confirmed this is not the case.
Thanks for any help you can provide,
Jamie
Question
Hi there,
I am interested in doing some parasitology study on these amazing, giant amphipods. Its a bit of a longshot but if anyone out there is able to get hold of some I would be very interested in looking at them. Species or location does not need to be specific. Any will do.
Any questions please post on here or contact me directly via my profile.
Thanks,
Jamie Bojko