James Butler

James Butler
University of Exeter | UoE · Medical School

BSc (Hons) MSc PhD

About

2
Publications
543
Reads
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0
Citations
Citations since 2017
2 Research Items
0 Citations
20172018201920202021202220230.00.20.40.60.81.0
20172018201920202021202220230.00.20.40.60.81.0
20172018201920202021202220230.00.20.40.60.81.0
20172018201920202021202220230.00.20.40.60.81.0
Introduction
Additional affiliations
September 2017 - July 2018
University of Plymouth
Position
  • Master's Student
June 2015 - July 2015
University of Plymouth
Position
  • Summer Scholarship Student
Education
September 2017 - July 2018
University of Plymouth
Field of study
  • Biomedical Science (Immunology)
September 2014 - June 2017
University of Plymouth
Field of study
  • Biomedical Science

Publications

Publications (2)
Article
Full-text available
Introduction. Cupriavidus pauculus is historically found in soil and water but has more recently been reported to cause human infection and death. Hospital sink traps can serve as a niche for bacterial persistence and a platform for horizontal gene trans- fer, with evidence of dissemination of pathogens in hospital plumbing systems driving nosocom...

Questions

Question (1)
Question
We are studying biofilm formation on differently coated 15mm diameter uPVC discs - these are opaque and may be white or black in colour depending on coating. The current setup includes forming biofilms on discs, rinsing to remove non-adherent cells, fixing each disc to a glass slide and then adding a drop of LIVE/DEAD BacLight mixture (Invitrogen L7012) to the centre of the disc. After incubation, the excess stain is rinsed off, a coverslip is placed on top of the disc and the edges of the coverslip fixed to the underlying slide with nail polish. After drying, the whole piece is placed in the inverted microscope coverslip side down. When imaging it is possible to see a hue of green or red depending on filter but not to resolve cells, and it is unclear whether this is just autofluorescence of the disc or coating. The opacity of the discs seems to block much of the light.
Thank you for your assistance and advice.

Network

Projects

Projects (3)
Project
Combining microbiology and nanomaterials, I am working on developing a nanocoating using silver nanoparticles for antibacterial applications in hospital sanitary plumbing environments.
Archived project
Investigate modulation of mucosal immunity by probiotic bacteria in the form of live and heat-killed bacteria, and their DNA. Focusing on pro-inflammatory cytokine production by differentiated macrophages, measured by sandwich enzyme-linked immunosorbent assay.
Archived project
Characterise the distribution of wildtype and mosaic tetracycline resistance genes in metagenomic environmental samples. Investigate a novel mosaic tetracycline resistance gene.