James E Bron

James E Bron
University of Stirling · Institute of Aquaculture

BSc, MSc, PhD

About

233
Publications
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Publications

Publications (233)
Article
Full-text available
Complex gill disorder (CGD) is an important condition in Atlantic salmon aquaculture, but the roles of the putative aetiological agents in the pathogenesis are uncertain. A longitudinal study was undertaken on two salmon farms in Scotland to determine the variations in loads of CGD-associated pathogens (Desmozoon lepeophtherii, Candidatus Branchiom...
Article
Full-text available
The pyrethroid deltamethrin (DTM) is used to treat Atlantic salmon ( Salmo salar ) against salmon louse ( Lepeophtheirus salmonis ) infestations. However, DTM resistance has evolved in L. salmonis and is currently common in the North Atlantic. This study aimed to re-assess the association between DTM resistance and mitochondrial (mtDNA) mutations d...
Article
The microsporidian Desmozoon lepeophtherii Freeman and Sommerville, 2009 is considered significant in the pathogenesis of gill disease in Atlantic salmon (Salmo salar Linnaeus, 1758). Due to the difficulty in detecting D. lepeophtherii in tissue sections, infections are normally diagnosed by molecular methods, routine haematoxylin and eosin (H&E) s...
Article
The relative rarity of the planktonic larval stages of salmon lice in comparison to other animals captured in a zooplankton assemblage is an obstacle to estimating their abundance and distribution. Due to the labour intensiveness of standard plankton sorting approaches, the planktonic stages of salmon lice remain understudied and unmonitored despit...
Article
Full-text available
Amyloodiniosis is a disease resulting from infestation by the ectoparasitic dinoflagellate Amylood-inium ocellatum (AO) and is a threat for fish species such as European sea bass (ESB, Dicentrarchus labrax), which are farmed in lagoon and land-based rearing sites. During the summer, when tem-peratures are highest, mortality rates can reach 100%, wi...
Article
Full-text available
Gill disorders have become more prevalent and widespread in finfish aquaculture in recent years. Their aetiology is often considered to be multifactorial. Effective diagnosis, control and prevention are hindered by the lack of standardised methodologies to characterise the aetiological agents, which produce an array of clinical and pathological pre...
Preprint
Full-text available
Deltamethrin (DTM) is used to treat Atlantic salmon ( Salmon salar ) against salmon lice ( Lepeophtheirus salmonis ) infestations. However, development of DTM resistance has been reported from North Atlantic L. salmonis populations, in which resistance is associated with mitochondrial (mtDNA) mutations. This study investigated the relationship betw...
Article
Full-text available
Genetic resistance to infectious pancreatic necrosis virus (IPNV) in Atlantic salmon is a rare example of a trait where a single locus (QTL) explains almost all of the genetic variation. Genetic marker tests based on this QTL on salmon chromosome 26 have been widely applied in selective breeding to markedly reduce the incidence of the disease. In t...
Article
The pyrethroid deltamethrin and the macrocyclic lactone emamectin benzoate (EMB) are used to treat infestations of farmed salmon by parasitic salmon lice, Lepeophtheirus salmonis. While the efficacy of both compounds against Atlantic populations of the parasite has decreased as a result of the evolution of resistance, the molecular mechanisms of dr...
Preprint
Full-text available
Background The genus Argulus, whose members are widely known as “fish lice”, comprises a group of obligate ectoparasites that cause argulosis. This parasitic disease affects both wild and cultured fish populations and can lead to significant mortalities. Our previous work confirmed the presence of immunomodulatory or other active products in the se...
Preprint
Full-text available
The genus Argulus comprises obligate ectoparasites known as “fish lice” that cause argulosis and significant mortalities in both wild and cultured fish populations. Our previous proteomic analysis confirmed the presence of immunomodulatory products in the secretory / excretory products (SEPs) of Argulus foliaceus (Linnaeus, 1758). In this study, hi...
Article
Full-text available
Monitoring of planktonic salmon louse (Lepeophtheirus salmonis salmonis) abundance and parameterization of key life-history traits has been hindered by labour-intensive and error-prone quantification using traditional light microscopy. Fluorescence illumination has been proposed as a means of improving visualization, but prior to this study adequat...
Article
The ectoparasite protozoan Amyloodinium ocellatum (AO) is the causative agent of amyloodiniosis in European seabass (ESB, Dicentrarchus labrax). There is a lack of information about basic molecular immune response mechanisms of ESB during AO infestation. Therefore, to compare gene expression between experimental AO-infested ESB tissues and uninfest...
Article
Full-text available
Background Gyrodactylus salaris Malmberg, 1957 is an OIE (Office International des Epizooties)-listed parasitic pathogen and had until the current study been reported from 19 countries across Europe, although many of these records require confirmation. The last comprehensive evaluation regarding the distribution of G. salaris , however, was made in...
Article
Full-text available
Background: Domestication is the process by which organisms become adapted to the human-controlled environment. Since the selection pressures that act upon cultured and natural populations differ, adaptations that favour life in the domesticated environment are unlikely to be advantageous in the wild. Elucidation of the differences between wild an...
Article
Full-text available
Background: The pyrethroid deltamethrin is used to treat infestations of farmed salmon by parasitic salmon lice, Lepeophtheirus salmonis (Krøyer). However, the efficacy of deltamethrin for salmon delousing is threatened by resistance development. In terrestrial arthropods, knockdown resistance (kdr) mutations of the voltage-gated sodium channel (N...
Article
Full-text available
Infectious pancreatic necrosis virus (IPNV) infection has been a major problem in salmonid aquaculture. Marker-assisted selection of individuals with resistant genotype at the major IPN quantitative trait locus (IPN-QTL) has significantly reduced mortality in recent years. We have identified host miRNAs that respond to IPNV challenge in salmon fry...
Article
Full-text available
Routine gill swabbing is a non-destructive sampling method used for the downstream qPCR detection and quantitation of the pathogen Neoparamoeba perurans, a causa-tive agent of amoebic gill disease (AGD). Three commercially available swabs were compared aiming their application for timelier AGD diagnosis (Calgiswab® (calcium alginate fibre-tipped),...
Article
Full-text available
Background: Gill health is one of the main concerns for Atlantic salmon aquaculture, and Amoebic Gill Disease (AGD), attributable to infection by the amoeba Neoparamoeba perurans, is a frequent cause of morbidity. In the absence of preventive measures, increasing genetic resistance of salmon to AGD via selective breeding can reduce the incidence o...
Article
Full-text available
Background: It is hypothesised that being a blood-feeding ectoparasite, Argulus foliaceus (Linnaeus, 1758), uses similar mechanisms for digestion and host immune evasion to those used by other haematophagous ecdysozoa, including caligid copepods (e.g. sea louse). We recently described and characterised glands associated with the feeding appendages...
Article
Full-text available
Neutrophils release nuclear chromatin decorated with antimicrobial proteins into the extracellular milieu as an innate immune defence mechanism to counter invading microbes. These chromatin structures, called extracellular traps (ETs) and released by a process called NETosis, have been detected in mammals, certain invertebrates and some fish specie...
Article
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Genomic selection enables cumulative genetic gains in key production traits such as disease resistance, playing an important role in the economic and environmental sustainability of aquaculture production. However, it requires genome-wide genetic marker data on large populations, which can be prohibitively expensive. Genotype imputation is a cost-e...
Article
Full-text available
Background: The salmon louse (Lepeophtheirus salmonis) infests farmed and wild salmonid fishes, causing considerable economic damage to the salmon farming industry. Infestations of farmed salmon are controlled using a combination of non-medicinal approaches and veterinary drug treatments. While L. salmonis has developed resistance to most availabl...
Poster
Full-text available
Amyloodinium ocellatum (AO) (Brown, 1931) is an ectoparasite protozoan belonging to the phylum Dinoflagellata with worldwide distribution and characterised by a very low species specificity. Amyloodiniosis represents a threat for European sea bass (Dicentrarchus labrax) farmed in lagoon and land-based rearing sites in the Mediterranean, especially...
Article
Full-text available
Amoebic gill disease (AGD) is emerging as one of the most significant health challenges affecting farmed Atlantic salmon in the marine environment. It is caused by the amphizoic amoeba Neoparamoeba perurans, with infestation of gills causing severe hyperplastic lesions, compromising overall gill integrity and function. This study used histology, tr...
Preprint
Full-text available
Background: Gill health is one of the main concerns for Atlantic salmon aquaculture, and Amoebic Gill Disease (AGD), attributable to infection by the amoeba Neoparamoeba perurans, is a frequent cause of morbidity. In the absence of preventive measures, increasing genetic resistance of salmon to AGD via selective breeding can reduce the incidence of...
Article
Full-text available
Two aqueous fixation methods (modified Davidson's solution and modified Davidson's solution with 2% (w/v) Alcian blue) were compared against two non‐aqueous fixation methods (methacarn solution and methacarn solution with 2% (w/v) Alcian blue) along with the standard buffered formalin fixation method to (a) improve preservation of the mucous coat o...
Article
Full-text available
The spotted sea hare Aplysia dactylomela Rang, 1828 is reported from four locationsin Libyan coastal waters. The direct observation reported here resulted from a field survey of sea snails in the coastal environs of Tripoli, to the west of the country, while the remaining records result from postings made on the Facebook™ social media website, made...
Article
Full-text available
The present paper documents the further spread of the invasive, non-indigenous sea snail Haminoea cyanomarginata Heller & Thompson, 1983 in the Mediterranean Sea. Since its original description in the Red Sea, this sea slug has been subsequently reported since 2001 from various locations throughout the Central, Eastern, and Western Mediterranean Se...
Article
Background Parasitic salmon lice (Lepeophtheirus salmonis) cause high economic losses in Atlantic salmon farming. Pyrethroids, which block arthropod voltage‐gated sodium channels (Nav1), are used for salmon delousing. However, pyrethroid resistance is common in L. salmonis. The present study characterised Nav1 homologues in L. salmonis in order to...
Article
Current sea louse models attempt to estimate louse burdens on wild and cultured salmon by predicting the production and distribution of lice larvae and estimating the risk of transmission. While physical characteristics of water bodies and weather can be accurately modelled, many aspects of sea lice biology require further parameterization. The aim...
Article
Full-text available
Background: The salmon louse, Lepeophtheirus salmonis, is an ectoparasitic copepod which feeds on the mucus, skin and blood of salmonid fish species. The parasite can persist on the surface of the fish without any effective control being exerted by the host immune system. Other ectoparasitic invertebrates produce compounds in their saliva, excreti...
Article
Ciliates have been reported as pathogens of many species of economically important bivalves. Mussel protozoan X (MPX), is an uncharacterised intracellular ciliate of mussels and has been widely reported in Mytilus spp. around the world. In order to characterise this ciliate, Mytilus edulis samples were collected from a site on the West coast of Sco...
Article
Full-text available
With increasing interest in the use of triploid salmon in commercial aquaculture, gaining an understanding of how economically important pathogens affect triploid stocks is important. To compare the susceptibility of diploid and triploid Atlantic salmon (Salmo salar L.) to viral pathogens, fry were experimentally infected with Salmonid alphavirus s...
Data
Skeletal muscle pathology. Pathological changes were observed in the intraperitoneally challenged fish. Inflammatory infiltration and degenerative changes were specifically present in the red muscle. (PDF)
Data
RT-qPCR result of SAV infected fish. The mean Cp value, mean estimated SAV copy number and infection states; positive (+ ve) and negative(-ve) for each individual fish. (PDF)
Data
Liver pathology. Severely damaged liver tissues stained with Haematoxylin and Eosin (H&E), periodic acid-schiff (PAS) and anti-human CD3 polyclonal antibody (CD3). The histological presentation suggest an ongoing peroxidative type of tissue damage and also possible virus induced hepatitis. (PDF)
Poster
AGD caused by Paramoeba perurans (Fig. 1), has become a significant problem for the Atlantic salmon farming industry worldwide1. Accuracy and sensitivity of its diagnosis is critical to timely treatment in a commercial situation. Calcium alginate fibre-tipped swabs offers an advantage, compared to the current tools used in the field, due to its c...
Article
Full-text available
Parasitic infections by the salmon louse, Lepeophtheirus salmonis (Krøyer), cause huge economic damage in salmon farming in the northern hemisphere, with combined treatment costs and production losses in 2014 having been estimated at US$ 350 million for Norway (annual production 1.25 million tonnes). The control of L. salmonis relies significantly...
Data
Geographic origin of salmon lice used to establish strains. Modified from a map created by N. Sinegina, used under a Creative Commons Attribution-Share Alike 4.0 License (http://www.supercoloring.com/silhouettes/scotland-map, accessed 9/5/2017). (PDF)
Data
Bioassay raw data. The table compiles the raw data used to calculate EC50 values shown in Table 1. (CSV)
Data
Actual deltamethrin concentration in L. salmonis bioassays. Bars show the average and standard deviation of results obtained for water samples taken at the beginning and the end of exposures (n = 3). (PDF)
Data
PCR primers to amplify L. salmonis mtDNA sequences. (CSV)
Data
Sequencing primers to sequence the above PCR products. (CSV)
Data
Full pedigree of L. salmonis subjected to mtDNA sequencing. For parasites derived from the crosses show in Fig 1 that were subjected to mtDNA sequencing, information is provided about strain origin of P0 animals and pedigree of F1 and F2 animals. (CSV)
Data
Deltamethrin susceptibility of L. salmonis F1 parasites derived from crosses. Symbols show the average percentage of parasites (n = 10–15) that were rated “affected” after 30 min of exposure to indicated deltamethrin concentrations and 24 h of recovery. (PDF)
Data
List of mtDNA sequencing reads. The table contains sample information and trimming coordinates for sequencing reads available under https://github.com/pseudogene/lice-mitochondria. (CSV)
Article
Full-text available
Genomic selection uses genome-wide marker information to predict breeding values for traits of economic interest, and is more accurate than pedigree-based methods. The development of high density SNP arrays for Atlantic salmon has enabled genomic selection in selective breeding programmes, alongside high-resolution association mapping of the geneti...
Article
Full-text available
Cyprinid herpesvirus 3 (CyHV-3), also called koi herpesvirus (KHV), is the aetiological agent of a fatal disease in carp and koi (Cyprinus carpio L.), referred to as koi herpesvirus disease. The virus contains at least 40 structural proteins, of which few have been characterised with respect to their immunogenicity. Indirect immunofluorescence assa...
Article
Full-text available
Background Due to selective breeding, domesticated and wild Atlantic salmon are genetically diverged, which raises concerns about farmed escapees having the potential to alter the genetic composition of wild populations and thereby disrupting local adaptation. Documenting transcriptional differences between wild and domesticated stocks under contro...
Article
Full-text available
Salmonid alphavirus (SAV) is an enveloped, single-stranded, positive sense RNA virus belonging to the family Togaviridae. It causes economically devastating disease in cultured salmonids. The characteristic features of SAV infection include severe histopathological changes in the heart, pancreas and skeletal muscles of diseased fish. Although the p...
Article
Full-text available
Background Sea lice have significant negative economic and welfare impacts on marine Atlantic salmon farming. Since host resistance to sea lice has a substantial genetic component, selective breeding can contribute to control of lice. Genomic selection uses genome-wide marker information to predict breeding values, and can achieve markedly higher a...
Article
Full-text available
Statement of relevance: Infections by sea lice cause significant costs to the global salmon farming industry, which have been estimated to exceed €300 million per year worldwide. Control of sea lice still relies to a significant extent on chemical delousing; however, chemical control is threatened by resistance formation. Resistance can be combate...
Article
Full-text available
The social media network Facebook™ was used to gather information on the occurrence and geographical distribution of dusky grouper dermatitis, a skin lesion affecting the dusky grouper, Epinephelus marginatus. Dusky grouper are common targets for spear fishermen in the Mediterranean and by monitoring spearfishing activity in Libyan waters, it was p...