Jakob Zubek

Jakob Zubek
Bielefeld University · CeBiTec - Center for Biotechnology

Master of Science

About

4
Publications
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Citations

Publications

Publications (4)
Poster
Full-text available
Final project poster from team Bielefeld-CeBiTec 2018 (nanoFactory) for the presentation at the iGEM Giant Jamboree 2018 from October 24th to 28th in Boston, Massachusetts, USA.
Presentation
Full-text available
A set of presentation slides to give an overview on the topics Biosecurity, Dual Use and Dual Use Research of Concern (DURC). We encourage everyone to use the open source slides to spread awareness and prevent issues concerning this topics.
Poster
Full-text available
Nanoparticles can be used for a wide range of applications. Especially if used in the medical field they need to be free from toxic chemicals, which are used in the conventional way of nanoparticle formation. Therefore, our goal is to use synthetic biology to create a way of generating environmentally friendly nanoparticles. Our Escherichia coli ta...
Poster
Full-text available
Nanoparticles can be used for a wide range of applications. Especially if used in the medical field they need to be free from toxic chemicals, which are used in the conventional way of nanoparticle formation. Therefore, our goal is to use synthetic biology to create a way of generating environmentally friendly nanoparticles. Our Escherichia coli ta...

Questions

Questions (3)
Question
Dear ResearchGate Community,
I guess the title says everything. For any scientific article I'll be very thankful.
With warm regards
Jakob Zubek
Question
Dear ResearchGate community,
what factors do I have to take in count to determine the prtoein expression rate of a certain promotor?
I thought of the
-RNA polymerase rate which binds to the promotor,
-the average NTP concentration under normal circumstances in a certain chassis (E.coli)
-the rate of translation from mRNA to Protein by ribosomes.
So the questions are:
1. Did I missed a point?
2. All of those factors are pretty much constants in one chassis, so is there maybe a certain name for the "expression constant for promotors" I miss or maybe a library of certain expression rates?
Thank you for your help.
Jakob Zubek
Question
Hello there,
i search for a trustworthy online database for spectroscopic data like f.e. the wavelength dependent of Ferric (III) acetate.
Any suggestions?
Thank you and greetings
Jakob Zubek

Projects

Project (1)
Project
Copper, silver and gold - metals are essential for our daily life but resources are dwindling. Industrial mining of metals and electronic waste cause pollution of the environment. Therefore, we established new approaches to recover valuable resources through synthetic biology. By enhancing bacterial abilities to scavenge metal ions from the environment we generated nanoparticles. We optimized Escherichia coli to accumulate metal ions as copper and iron by overexpression of dedicated importers and silencing of exporters while reducing the effects of oxidative stress. To gather nanoparticles from various metal ions we engineered the iron storage protein ferritin. Recycled into nanoparticles the metals could be used for various applications as demonstrated by printing electronics. Considering Dual Use aspects we decided to extract metal ions from pit water instead of dissolving electronics directly. Therefore, in close collaboration with leading experts we developed a customized cross-flow bioreactor for the mining industry.