Jaipal Panga

Jaipal Panga
Institute for Systems Biology · Moritz Lab

PhD

About

52
Publications
13,321
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Citations
Introduction
I am Jaipal Reddy Panga working as Postdoctoral Fellow (infectious disease bio-marker and proteomics) at Institute for Systems Biology, Seattle. My major focus is to discover early disease diagnosis bio-makers for Tick-borne infectious diseases. I am working on quite a few clinical isolates of lyme causing bacteria isolated from different parts of USA and doing multi-omic analysis such as genomics, transcriptomics and proteomics to understand the disease and design diagnostic markers.
Additional affiliations
June 2015 - June 2016
Indian Institute of Technology Bombay
Position
  • Researcher
January 2010 - present
Indian Institute of Technology Bombay
Position
  • Proteomic analysis of antimicrobial agents to understand the mechanism of action
January 2010 - June 2016
Indian Institute of Technology Bombay
Position
  • Researcher
Education
June 2006 - June 2008
Independent Researcher
Independent Researcher
Field of study
  • Biochemistry

Publications

Publications (52)
Article
Full-text available
Labeo rohita (Rohu) is one of the most important fish species produced in world aquaculture. Integrative omics research provides a strong platform to understand the basic biology and translate this knowledge into sustainable solutions in tackling disease outbreak, increasing productivity and ensuring food security. Mass spectrometry-based proteomic...
Article
Full-text available
Lyme disease (also known as Lyme borreliosis) is the most common vector-borne disease in the United States with an estimated 476,000 cases per year. While historically, the long-term impact of Lyme disease on patients has been controversial, mounting evidence supports the idea that a substantial number of patients experience persistent symptoms fol...
Article
Full-text available
Data-Independent Acquisition (DIA) is a method to improve consistent identification and precise quantitation of peptides and proteins by mass spectrometry (MS). The targeted data analysis strategy in DIA relies on spectral assay libraries that are generally derived from a priori measurements of peptides for each species. Although Escherichia coli (...
Article
The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for antimalarial drugs and vaccines. Here we provide the first...
Article
Research question: A close association between Kisspeptin-1 (KISS-1) and reproductive physiology has been reported, but the results on circulatory KISS-1 are ambiguous in patients with polycystic ovary syndrome (PCOS). A systematic review and meta-analysis were conducted to evaluate the association between KISS-1 and PCOS, and to test its diagnost...
Preprint
The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for anti-malarial drugs and vaccines. Here we provide the first...
Article
The association between circulating betatrophin levels and polycystic ovary syndrome (PCOS) is controversial and the studies in the literature are inconsistent. The aim of our study was to systematically review and meta-analyze all available literature comparing circulating betatrophin levels between human PCOS patients and controls. Relevant studi...
Article
The cover image represents a proteomics study of parasites isolated from infected red blood cells (RBCs). Infected RBCs are shown as dull red structures compared to the normal healthy ones which are bright red. The figure depicts the isolation of infected RBCs followed by protein extraction and mass spectrometry‐based identification of proteins. Th...
Article
Full-text available
Lyme disease is caused by spirochaetes of the Borrelia burgdorferi sensu lato genospecies. Complete genome assemblies are available for fewer than ten strains of Borrelia burgdorferi sensu stricto, the primary cause of Lyme disease in North America. MM1 is a sensu stricto strain originally isolated in the midwestern United States. Aside from a smal...
Data
Genome-scale dotplots of Bb MM1 and B31. A) Dotplot of MM1 versus B31 shows a very high degree of similarity. MM1 contains a subset of the plasmids of B31. B) Dotplot of MM1 versus itself illustrates two characteristics of Borrelia genomes. First, the cp32 family of plasmids show great similarity. Second, a large repetitive region, the vls locus, a...
Data
Nanopore sequencing reads confirm the novel SNPs in nifS and pepX. Top: The de novo assembly of the nifS gene matches the MLST nifS type 12 except for a C->T polymorphism. Eleven nanopore reads align to this region and all contain the T variant. Bottom: The de novo assembly of the pepX gene matches the MLST pepX type 1 except for a T->C polymorphis...
Data
MM1 gene members of PFam32, PFam49, PFam50 and PFam57/62 identified by spectral clustering of protein sequences. The table lists the results of SCPS analysis for the 4 PFams 32, 49, 50 and 57/62 (see Materials and methods) in MM1, N40, JD1 and B31 strains. Genes located on replicons of the same type are arranged in nearby rows within each paralogou...
Data
Coverage of MM1 sequence reads across B. burgdorferi B31 plasmids. An initial mapping of MM1 reads to the type strain B31 suggested lack of plasmids lp21, lp28-1, lp28-2, lp38, lp56 and lp5 in MM1 genome, while the presence/absence status of other plasmids in MM1 remained ambiguous. B31 plasmids are shown in each plot with X-axis and Y-axis represe...
Article
Full-text available
Borrelia burgdorferi, a bacterium in the spirochete phylum, is the causative agent of Lyme disease. B. burgdorferi has a linear chromosome with a number of circular and linear plasmids. Bacteria, including B. burgdorferi, release spherical outer membrane vesicles (OMVs) that are known to carry secretory products including metabolites, nucleic acids...
Article
Full-text available
Adenocarcinomas are cancers originating from the gland forming cells of the colon and rectal lining, and are known to be the most common type of colorectal cancers. The current diagnosis strategies for colorectal cancers include biopsy, laboratory tests, and colonoscopy which are time consuming. Identification of protein biomarkers could aid in the...
Preprint
Lyme disease is caused by spirochaetes of the Borrelia burgdorferi sensu lato genospecies. Complete genome assemblies are available for fewer than ten strains of Borrelia burgdorferi sensu stricto, the primary cause of Lyme disease in North America. MM1 is a sensu stricto strain originally isolated in the midwestern United States. Aside from a smal...
Article
Purpose: Plasmodium vivax is the most geographically widespread species responsible for malaria in humans. Our study focused on identifying highly expressed parasite proteins using a shotgun proteomics approach. Experimental design: Parasites (P. vivax) were isolated from 7 patient samples using saponin lysis. Protein extracts from these parasit...
Chapter
Mass spectrometry (MS) is the method of choice for both qualitative and quantitative high-throughput proteome analysis. In the early years, mass spectrometry was used only for small molecule analysis. However, advances in ionization sources, mass analyzers, and mass detectors made MS the central force in proteomics technologies. Starting from its u...
Chapter
The innovations in genome sequencing technologies have emanated in better understanding of biosystems leading to the dawn of the “omics” era. Proteomics has been an integral interface in the post-genomic era, and has allowed researchers to explore other omics-based platforms like metabolomics, transcriptomics, phenomics, etc. In pursuit of obtainin...
Article
Full-text available
Proteomics is at the epicenter of post-genomics biotechnologies that are currently driving the next generation system science. Moreover, proteomics is a truly global science. The 6th Annual Meeting of Proteomics Society, India (PSI) and International Conference on “Proteomics from Discovery to Function” held from December 7–9, 2014, was a transform...
Article
Given the diverse range of transcriptional and post-transcriptional mechanisms of gene regulation, the estimates of the human proteome is likely subject to scientific surprises as the field of proteomics has gained momentum worldwide. In this regard, the establishment of the "Human Proteome Draft" using high-resolution mass spectrometry (MS), tissu...
Article
Full-text available
Curcumin is a natural dietary compound with antimicrobial activity against various gram positive and negative bacteria. This study aims to investigate the proteome level alterations in Bacillus subtilis due to curcumin treatment and identification of its molecular/cellular targets to understand the mechanism of action. We have performed a comprehen...
Article
Full-text available
Abstract Infectious diseases and increasing antibiotic resistance among diverse classes of microbes are global health concerns and a prime focus of omics systems science applications in novel drug discovery. Plumbagin is a plant-derived naphthoquinone, a natural product that exhibits antibacterial activity against gram-positive bacteria. In the pre...
Article
Unlabelled: The rapid emergence of microbial drug resistance indicates the urgent need for development of new antimicrobial agents. Bacterial cell division machinery is considered as a promising antimicrobial target. Totarol is a naturally existing diterpenoid, which has the ability to restrain bacterial growth by perturbing the cell division. The...
Article
Full-text available
Abstract Metadata refer to descriptions about data or as some put it, "data about data." Metadata capture what happens on the backstage of science, on the trajectory from study conception, design, funding, implementation, and analysis to reporting. Definitions of metadata vary, but they can include the context information surrounding the practice o...
Article
Full-text available
The success of a proteomic experiment largely depends on the quality and quantity of the protein extract. Currently, various protocols are available for extraction of proteins from different types of samples; however, further optimization is required for every new sample type. Hence, a common protein extraction protocol is desirable. In the present...
Article
Full-text available
Web-based educational resources have gained enormous popularity recently and are increasingly becoming a part of modern educational systems. Virtual Labs are E-learning platforms where learners can gain the experience of practical experimentation without any direct physical involvement on real bench work. They use computerized simulations, models,...
Article
Analysis of different biological fluids that contain an archive of information regarding the pathophysiological conditions of a patient is very useful for biomarker discovery and early disease diagnosis. In recent years there are intense efforts to analyze various biological fluids and identify panels of protein markers for different types of cance...
Article
Full-text available
The Millennium Summit in 2000 established the Millennium Development Goals (MDGs), which were agreed upon by 193 countries and 23 international organizations to combat extreme poverty and other pressing global priorities for human development. In the December 2011 issue of CPPM, Borda-Rodriguez and Huzair present an analysis of the close ties and s...
Article
Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenesis makes serum an attractive sa...
Article
Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenesis makes serum an attractive sa...
Article
Availability of genome sequence of human and different pathogens has advanced proteomics research for various clinical applications. One of the prime goals of proteomics is identification and characterization of biomarkers for cancer and other fatal human diseases to aid an early diagnosis and monitor disease progression. However, rapid detection o...
Article
Full-text available
The field of proteomics has undergone rapid advancements over the last decade and protein microarrays have emerged as a promising technological platform for the challenging task of studying complex proteomes. This gel-free approach has found an increasing number of applications due to its ability to rapidly and efficiently study thousands of protei...

Questions

Questions (3)
Question
I want to measure the concentration of curcumin in DMSO. So for the same I need the molar extinction coefficient for curcumin in DMSO.
Question
Can anyone advice on how to use the "The SEED: an Annotation/Analysis Tool Provided by FIG" database? I have a data to annotate with this database.
Question
Is it possible to get more than one protein identification from single 2-DE spot. If so, please suggest some papers where these kinds of results are published......

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Projects

Projects (2)
Archived project
Project
Biomarker discovery for early diagnosis of infectious diseases using advanced proteomic tools such as SRM, SWATH