Jacques Fattaccioli

Jacques Fattaccioli
Ecole Normale Supérieure de Paris | ENS

Professor

About

47
Publications
10,872
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
428
Citations
Citations since 2016
30 Research Items
259 Citations
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
Introduction
Our laboratory is specialized in sophisticated emulsion-based materials design and microfluidics. It is part of ENS - PSL, a research intensive university ranking first among the french higher education institutions in the Shanghai Ranking. Additionally, we are part of Institut Pierre-Gilles de Gennes, which is focused on microfluidic technologies.
Additional affiliations
September 2009 - present
Sorbonne Université
Position
  • Professor (Associate)
November 2006 - February 2008
The University of Tokyo
Position
  • Research Associate
September 2002 - September 2006
École Supérieure de Physique et de Chimie Industrielles
Position
  • PhD Student
Education
September 2002 - September 2006
Sorbonne Université
Field of study
  • Emulsion science and Biophysics
September 2001 - June 2002
Sorbonne Université
Field of study
  • Soft Condensed Matter and Chemical Physics
September 1998 - June 2002

Publications

Publications (47)
Article
Full-text available
Background: Plant protoplasts are basic plant cells units in which the pecto-cellulosic cell wall has been removed, but the plasma membrane is intact. One of the main features of plant cells is their strong plasticity, and their propensity to regenerate an organism from a single cell. Methods and differentiation protocols used in plant physiology...
Article
Full-text available
Phagocytosis by macrophages represents a fundamental process essential for both immunity and tissue homeostasis. It consists in the uptake of pathogenic or cellular targets larger than 0.5 μm. For the biggest particles, the phagocytic process involves a massive reorganization of membrane and actin cytoskeleton as well as an important intracellular...
Article
Full-text available
Alcanivorax borkumensis is a ubiquitous marine bacterium that utilizes alkanes as a sole carbon source. We observed two phenotypes in the A. borkumensis SK2 type strain: rough (R) and smooth (S) types. The S type exhibited lower motility and higher polysaccharide production than the R type. Full genome sequencing revealed a mutation in the S type i...
Article
This activity introduces droplet-based millifluidics and the usage of aqueous droplets as independent chemical microreactors for reaction-kinetics studies. Students build their own droplet generators from common macroscopic glassware and connecting parts to create trains of millimeter-sized droplets in which a redox reaction takes place. The activi...
Article
Full-text available
Characterized by a fluid and deformable interface, ligand-functionalized emulsion droplets are used as model probes to address biophysical, biological, and developmental questions. Functionalization protocols usually rely on the use of headgroup-modified phospholipids that are dissolved in the oil phase prior to emulsification, leading to a broad r...
Article
Full-text available
The immune synapse is the tight contact zone between a lymphocyte and a cell presenting its cognate antigen. This structure serves as a signaling platform and entails a polarization of intracellular components necessary to the immunological function of the cell. While the surface properties of the presenting cell are known to control the formation...
Article
Full-text available
Immune synapse formation is a key step for lymphocyte activation. In B lymphocytes, the immune synapse controls the production of high-affinity antibodies, thereby defining the efficiency of humoral immune responses. While the key roles played by both the actin and microtubule cytoskeletons in the formation and function of the immune synapse have b...
Preprint
Full-text available
Alcanivorax borkumensis are prominent actors in oil spill bioremediation; however, the interfacial dynamics of their biofilms and its role in oil degradation remain unclear. Longitudinal tracking of biofilm-covered oil microdroplets using microfluidics reveals a spontaneous morphological transition from a thick biofilm phenotype to a thin dendritic...
Preprint
Full-text available
Immune synapse formation is a key step for lymphocyte activation. In B lymphocytes, the immune synapse controls the production of high-affinity antibodies, thereby defining the efficiency of humoral immune responses. While the key roles played by both the actin and microtubule cytoskeletons in the formation and function of the immune synapse have b...
Preprint
Full-text available
The immune synapse is the tight contact zone between a lymphocyte and a cell presenting its cognate antigen. This structure serves as a signaling platform and entails a polarization of intra-cellular components, necessary to the immunological function of the cell. While the surface properties of the presenting cell are known to control the formatio...
Article
Full-text available
With the aim to parallelize and monitor biological or biochemical phenomena, trapping and immobilization of objects such as particles, droplets or cells in microfluidic devices has been an intense area of research and engineering so far. Either being passive or active, these microfluidic devices are usually composed of arrays of elementary traps wi...
Preprint
Microfluidic Trap Arrays (MTAs) appear as very promising tools for several applications like cancer understanding and treatment or immune synapse research. The MTAs principle is the following: samples are diluted inside a fluid going through a microfluidic chamber composed of an array of a large number of traps. While following the fluid flow, samp...
Preprint
With the aim to parallelize and monitor biological or biochemical phenomena, trapping and immobilization of objects such as particles, droplets or cells in microfluidic devices has been an intense area of research and engineering so far. Either being passive or active, these microfluidic devices are usually composed of arrays of elementary traps wi...
Article
Full-text available
In this work we report on the development of mannose-coated fluorescent lipid microparticles to study the role of C-type lectin membrane receptor in phagocytosis. The micrometric droplets of Soybean oil in water emulsion were functionalized with a tailor-made fluorescent mannolipid. The amphiphilic ligand was built from a mannose unit, a lipid C11...
Preprint
Full-text available
The Shigella effector IpaA co-opts the focal adhesion protein vinculin to promote bacterial invasion. Here, we show that IpaA triggers an unreported mode of vinculin activation through the cooperative binding of its three vinculin-binding sites (VBSs) leading to vinculin oligomerization via its D1 and D2 head subdomains and highly stable adhesions...
Preprint
Full-text available
Background: One of the main features of plant cells is their strong plasticity, and their propensity to regenerate an organism from a single cell. Plant protoplasts are basic plant cells units in which the pecto-cellulosic cell wall has been removed, but the plasma membrane is intact. One of the main features of plant cells is their strong plastici...
Preprint
div> This activity introduces digital microfluidics and the usage of aqueous droplets as independent chemical microreactors for reaction kinetics studies. Students build their own droplet generator from common macroscopic glassware and connecting parts, to create trains of millimetric droplets in which a redox reaction takes place. The activity al...
Preprint
Phagocytosis by macrophages represents a fundamental process essential for both immunity and tissue homeostasis. It consists in the uptake of pathogenic or cellular targets larger than 0.5μm. For the biggest particles, the phagocytic process involves a massive reorganization of membrane and actin cytoskeleton as well as an important intracellular d...
Article
A new multi-compartment microfluidic device was developed to simultaneously desalinate and recover valuable metal ions from aqueous streams mimicking metal plating and mining tailing wastewaters. Heavy and valuable metal ions including copper, zinc, nickel, silver and zinc/copper ionic mixtures were selectively transferred from the feed solution to...
Article
Aim: Formulate nanometric oil droplets for encapsulating solid nanoparticles and assess their interactions with cells. Materials & methods: Soybean oil droplets, stabilized by Pluronic F68 surfactant, incorporating hydrophobically modified fluorescent silica, nanoparticles were obtained. Cytotoxicity over time, internalization, subsequent intrac...
Article
Full-text available
The ability of immune cells to migrate within narrow and crowded spaces is a critical feature involved in various physiological processes from immune response to metastasis. Several in-vitro techniques have been developed so far to study the behaviour of migrating cells, the most recent being based on the fabrication of microchannels within which c...
Article
Fluorescent latex beads have been widely used as tracers in microfluidics over for the last decades. They have the advantages to be density matched with water and to be easily localizable using fluorescence microscopy. We have recently synthesized silver-coated oil droplets that are both luminescent and absorptive, by first coating the oil interfac...
Research
Full-text available
ABSTRACT: Fluorescent latex beads have been widely used as tracers in microfluidics over for the last decades. They have the advantages to be density matched with water and to be easily localizable using fluorescence microscopy. We have recently synthesized silver-coated oil droplets that are both luminescent and absorptive, by first coating the oi...
Article
Polydopamine (PDA), a multifunctional biomaterial with strong adhesion and coating properties, exhibits melanin-like optoelectronic properties but is virtually devoid of intrinsic fluorescence. Herein we disclose the first PDA-based system that can develop fluorescence without chemical manipulation. The polymerization of dopamine in the presence of...
Article
Full-text available
We have developed a simple fabrication process for fluorescent silver-coated liquid particles. The synthesis involves the encapsulation of quasi-monodisperse micronic soybean oil-in-water emulsion droplets in a polydopamine shell followed by an electroless silver plating of the surface. Due to the presence of the thin silver layer, the droplets exh...
Article
Full-text available
In this paper, we investigated the localized anodization of aluminum evaporated on silicon substrate using micro scale patterns. Positive (S1818) and negative (SU-8 2035, KMPR 1010, KMPR 1035) photoresist patterns as well as SiO2 micro patterns were fabricated on aluminum substrates respectively. Then, anodization of each substrate was performed in...
Article
Full-text available
We demonstrate the active transport of liquid cargos in the form of oil-in-water emulsion droplets loaded on kinesin motor proteins moving along oriented microtubules. We analyze the motility properties of the kinesin motors (velocity and run length) and find that the liquid cargo in the form of oil droplets does not alter the motor function of the...
Article
Full-text available
Biotechnological applications of emulsions, such as micro-reactors or drug carriers, demand accurate characterization techniques, able to measure the size and biochemical content of the droplets at the individual level. Since no available characterization technique completely fulfills these needs, we extended the use of flow cytometry, which was or...
Conference Paper
Full-text available
In this paper we present the results of surface temperature control of silicon nanowires by using fluorescent thermometry at the nanometer scale. Rhodamine B is one of the stable fluorescent molecules, which rely on the characteristic of temperature-dependent change in fluorescent intensity, and it was used for nano-scale surface temperature sensin...
Conference Paper
Full-text available
Microcontact printing has been shown to be a viable lithographic technique for the fabrication of microstructures, through the deposition of molecules by conformal contact between a surface and an elastomeric stamp. However, the diffusion of the molecules on the substrate and the deformation of the stamp during the contact are severe drawbacks when...
Article
Full-text available
We have produced emulsion droplets of controlled size and composition coated by ligands, and studied the adhesion of these drops on a solid substrate coated by receptors and polymers. Using transmission, RICM and fluorescence microscopy we assess the size, contact angle and ligand density for each drop. We first show that non-specific interactions...
Conference Paper
Full-text available
A hybrid bio-electrical system for direct transportation and electrofusion of oil droplets is proposed. A motor protein system, kinesin/microtubule, was used to transport kinesin-coated oil droplets along microtubules oriented and immobilized on a glass surface. The attachment between the biotinylated-kinesin and the functionalized oil droplet was...
Article
Full-text available
We explored the ability of emulsion droplets to bind specifically solid model substrates and we study the relationship between drop composition and the contact angle. We developed a in-sisu method to graft covalently biotin and streptavidin on the O/W emulsion interface. We characterized the size distribution and the surface density of the streptav...
Thesis
Full-text available
Nous avons exploré la capacité des émulsions à adhérer de manière spécifique à des surfaces modèles solides et notamment la relation entre la composition des gouttes à leur interface et la formation d'un angle de contact macroscopique. Nous avons développé une méthode de fonctionnalisation covalente in-situ de l'interface liquide de gouttes d'émuls...
Article
Oils containing both fluorescent semiconductor and magnetic oxide nanoparticles are used to produce oil in water emulsions. This technique produces oil droplets with homogeneous fluorescence and high magnetic nanoparticle concentrations. The optical properties of the oil droplets are studied as a function of the droplet sizes for various concentrat...

Questions

Question (1)
Question
Dear all, 
After some months of procrastination, we changed 4/5 valves of our Accuri C6 (the ones that were not working), and I've started to check how the instrument is making measurements.
I have 2 issues for which I need help ;
- First, the instrument is unable to calibrate the fluidics. I thought the valve replacement would improve that point, but finally not. Do you have a hint  ?
- When I run the C6 with 0.2µm DI water, the recordings look like the attached picture. The threshold value is 80000 on the FSC-H channel. The event rate is close to 4000/s, and do not depend on the flow rate. 
Could you provide us any help ?
Thank you very much
Jacques FATTACCIOLI
UMR PASTEUR, Département de Chimie Ecole Normale Supérieure http://jacquesfattaccioli.wordpress.com https://microfluidics-master.fr Current address :  Institut Pierre-Gilles de Gennes 6 rue Jean Calvin 75005 Paris Tel/Fax : +33 1 40 79 58 92

Network

Cited By

Projects

Projects (3)
Project
Plants and Algae in Microfluidics
Project
Oil-eating bacteria
Project
The goal of the project is to use functional lipid droplets, namely emulsions, as biological and mechanical probes in the context of immunology.