J. Gerritse

J. Gerritse
Deltares

PhD

About

91
Publications
10,225
Reads
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2,263
Citations
Additional affiliations
January 2010 - present
Deltares
Position
  • Specialist microbiology
January 2003 - present
Wageningen University & Research
January 1998 - December 2010
TNO
Position
  • Scientist / project leader

Publications

Publications (91)
Article
Full-text available
The anaerobic degradation of aromatic hydrocarbons in a plume originating from a Pintsch gas tar-DNAPL zone was investigated using molecular, isotopic- and microbial analyses. Benzene concentrations diminished at the relatively small meter scale dimensions of the nitrate reducing plume fringe. The ratio of benzene to toluene, ethylbenzene, xylenes...
Article
Full-text available
A key question in microbial ecology is what the driving forces behind the persistence of large biodiversity in natural environments are. We studied a microbial community with more than 100 different types of species which evolved in a 15-years old bioreactor with benzene as the main carbon and energy source and nitrate as the electron acceptor. Usi...
Article
As the use of hydrogen gas (H2) as a renewable energy carrier experiences a major boost, one of the key challenges for a constant supply is safe and cost-efficient storage of surplus H2 to bridge periods with low energy demand. For this purpose, underground gas storage (UGS) in salt caverns, deep aquifers and oil-/gas reservoirs has been proposed,...
Preprint
As the use of hydrogen gas (H2) as a renewable energy carrier experiences a major boost, one of the key challenges for a constant supply is safe and cost-efficient storage of surplus H2 to bridge periods with low energy demand. For this purpose, underground gas storage (UGS) in salt caverns, deep aquifers and oil-/gas reservoirs has been proposed,...
Preprint
Full-text available
A key question in microbial ecology is what the driving forces behind the persistence of large biodiversity in natural environments are. We studied a microbial community with more than 100 different types of species which evolved in a 15-years old bioreactor with benzene as the main carbon and free energy source and nitrate as the electron acceptor...
Article
Full-text available
We studied the fragmentation of conventional thermoplastic and compostable plastic items in a laboratory seawater microcosm. In the microcosm, polyurethane foams, cellulose acetate cigarette filters, and compostable polyester and polylactic acid items readily sank, whereas polyethylene air pouches, latex balloons, polystyrene foams and polypropylen...
Article
Full-text available
Source zones containing tar, a dense non-aqueous phase liquid (DNAPL), can contaminate groundwater for centuries. A common occurrence of tar is at former Pintsch gas factories. Little is known about the composition and fate of contaminants dissolving from Pintsch gas tar DNAPL. In this study, we determined the composition and water-soluble characte...
Article
Denitrifying bioreactors are dependent on organic matter supply as a substrate for effective NO3 removal. In this study, the difference in removal efficiency and side effects when using different organic matter sources and dosing strategies was tested in two field experiments. The organic matter sources tested were woodchips and ethanol. The effect...
Article
EtBE is a fuel oxygenate that is synthesized from (bio)ethanol and fossil-based isobutylene, and replaces the fossil-based MtBE. Biodegradation of EtBE to harmless metabolites or end products can reduce the environmental and human health risks after accidental release. In this study, an algal-bacterial culture enriched from contaminated groundwater...
Article
Full-text available
The increasing use of biobased fuels and fuel additives can potentially change the typical fuel-related contamination in soil and groundwater. Anaerobic biotransformation of the biofuel additive ethyl tert-butyl ether (EtBE), as well as of methyl tert-butyl ether (MtBE), benzene, and tert-butyl alcohol (TBA, a possible oxygenate metabolite), was st...
Article
Full-text available
In this study, we report transcription of genes involved in aerobic and anaerobic benzene degradation pathways in a benzene-degrading denitrifying continuous culture. Transcripts associated with the family Peptococcaceae dominated all samples (21–36% relative abundance) indicating their key role in the community. We found a highly transcribed gene...
Article
Full-text available
Benzene is an aromatic compound and harmful for the environment. Biodegradation of benzene can reduce the toxicological risk after accidental or controlled release of this chemical in the environment. In this study, we further characterized an anaerobic continuous biofilm culture grown for more than 14 years on benzene with nitrate as electron acce...
Conference Paper
Full-text available
Uncertainties are present about the mechanisms of cathodic protection (CP) and its effectiveness to limit or completely stop Microbiologically Influenced Corrosion (MIC). The goal of this research was to improve the understanding of the mechanisms of CP by determining the interactions between corrosion and local chemical parameters, such as pH, und...
Article
Full-text available
p>Benzene is an aromatic compound and harmful for the environment. Biodegradation of benzene can reduce the toxicological risk after accidental or controlled release of this chemical in the environment. In this study, we further characterized an anaerobic continuous biofilm culture grown for more than 14 years on benzene with nitrate as electron ac...
Patent
Full-text available
The present invention relates to a system for detecting microbial contamination of a liquid specimen comprising a device for concentrating micro-organisms from a liquid specimen, having (i) a hypobaric chamber, (ii) a filter housing comprising a liquid-permeable bed of an adsorbent material and adapted for being fluidly connected to said hypobaric...
Article
Full-text available
The genomes of the Betaproteobacteria Alicycliphilus denitrificans strains BC and K601(T) have been sequenced to get insight into the physiology of the two strains. Strain BC degrades benzene with chlorate as electron acceptor. The cyclohexanol-degrading denitrifying strain K601(T) is not able to use chlorate as electron acceptor, while strain BC c...
Data
List of genes involved in the citric acid cycle and acetate metabolism in A. denitrificans strains BC and K601T. (DOCX)
Data
List of genes involved in degradation of aromatic and acyclic compounds in A. denitrificans strains BC and K601T. (DOCX)
Data
Major cellular fatty acids of A. denitrificans strains BC and K601T. Data of a previous study of strain K601T [6] are included. (DOCX)
Data
Location of the 16S and 23S rRNA genes in the genomes of A. denitrificans strains BC and K601T. (DOCX)
Data
List of genes involved in anaerobic respiration in A. denitrificans strains BC and K601T. (DOCX)
Article
An anaerobic microbial community was enriched in a chemostat that was operated for more than 8 years with benzene and nitrate as electron acceptor. The coexistence of multiple species in the chemostat and the presence of a biofilm, led to the hypothesis that benzene-degrading species coexist in a syntrophic interaction, and that benzene can be degr...
Article
An anaerobic microbial community was enriched in a chemostat that was operated for more than 8 years with benzene and nitrate as electron acceptor. The coexistence of multiple species in the chemostat and the presence of a biofilm, led to the hypothesis that benzene-degrading species coexist in a syntrophic interaction, and that benzene can be degr...
Article
Full-text available
Alicycliphilus denitrificans strain BC and A. denitrificans strain K601T degrade cyclic hydrocarbons. These strains have been isolated from a mixture of wastewater treatment plant material and benzene-polluted soil and from a wastewater treatment plant, respectively, suggesting their role in bioremediation of soil and water. Although the strains ar...
Article
Groundwater is a major source for drinking water, because of its good microbial quality in its natural state as compared with fresh surface water. Nevertheless, it may be contaminated with pathogenic microorganisms, especially viruses, and that may hamper drinking water production. The two most significant processes controlling virus mobility in th...
Article
River systems are exposed to anthropogenic disturbances, including chemical pollution and eutrophication. This may affect the phylogenetic diversity as well as the abundance of various functional groups within sediment-associated microbial communities. To address such potential effects, mesocosms filled with Ebro delta sediment covered with river w...
Article
Full-text available
Objectives of this work are to investigate effects of pH and ionic strength (IS) on virus transport in saturated soil and to develop a quantitative relationship for these effects. A series of 50-cm column experiments with clean quartz sand under saturated conditions and with pH values of 5, 6, 7, 8, and IS values of 1, 10, and 20 mM were conducted....
Article
Quantitative analysis of genes that code for Dehalococcoides 16S rRNA and chloroethene reductive dehalogenases TceA, VcrA and BvcA was done on groundwater sampled from 150 monitoring wells spread over 11 chlorinated ethene polluted European locations. Redundancy analysis (RDA) was used to relate molecular data to geochemical conditions. Dehalococco...
Article
River systems are exposed to anthropogenic disturbances, including chemical pollution and eutrophication. This may affect the phylogenetic diversity as well as the abundance of various functional groups within sediment-associated microbial communities. To address such potential effects, mesocosms filled with Ebro delta sediment covered with river w...
Article
Full-text available
Quantitative analysis of genes that code for Dehalococcoides 16S rRNA and chloroethene-reductive dehalogenases TceA, VcrA, and BvcA was done on groundwater sampled from 150 monitoring wells spread over 11 chlorinated ethene polluted European locations. Redundancy analysis was used to relate molecular data to geochemical conditions. Dehalococcoides...
Article
Full-text available
Aromatic hydrocarbons are widespread in nature and often contribute to the pollution of soils, sediments, and groundwater. The contamination of soil with mobile aromatic compounds, generally termed BTEX (benzene, toluene, ethylbenzene, xylene) is observed at many industrial sites, especially those associated with the petrochemical industry. In situ...
Chapter
IntroductionSorption of Micro-organics in GroundwaterBiodegradation of Micro-organics in GroundwaterAromatic HydrocarbonsMethyl Tert-Butyl Ether (MTBE)Chlorinated AliphaticsChlorinated AromaticsSubstituted PhenolsPesticidesOther Organic CompoundsConclusions and OutlookReferences
Article
Insight into the pathways of biodegradation and external factors controlling their activity is essential in adequate environmental risk assessment of chlorinated aliphatic hydrocarbon pollution. This study focuses on biodegradation of 1,2-dichloroethane (1,2-DCA) in microcosms containing sediment sourced from the European rivers Ebro, Elbe and Danu...
Article
Full-text available
AquaTerra is one of the first environmental projects within the 6th Framework programme by the European Commission. It began in June 2004 with a multidisciplinary team of 45 partner organizations from 13 EU countries, Switzerland, Serbia, Romania and Montenegro. Results from sampling and modeling in 4 large river basins (Ebro, Danube, Elbe and Meus...
Article
Full-text available
AquaTerra is one of the first environmental projects within the 6th Framework program by the European Commission. It began in June 2004 with a multidisciplinary team of 45 partner organizations from 13 EU countries, Switzerland, Serbia, Romania and Montenegro. Results from sampling and modeling in four large river basins (Ebro, Danube, Elbe and Meu...
Article
Full-text available
A bacterium, strain BC, was isolated from a benzene-degrading chlorate-reducing enrichment culture. Strain BC degrades benzene in conjunction with chlorate reduction. Cells of strain BC are short rods that are 0.6 microm wide and 1 to 2 microm long, are motile, and stain gram negative. Strain BC grows on benzene and some other aromatic compounds wi...
Article
A stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. The benzene degradation rate was 1.65 mM benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. Denaturing gradient gel electrophoresis of par...
Article
The pathway of 2-chloroethanol degradation in the denitrifying Pseudomonas stutzeri strain JJ was investigated. In cell-free extracts, activities of a phenazine methosulfate (PMS)-dependent chloroethanol dehydrogenase, an NAD-dependent chloroacetaldehyde dehydrogenase, and a chloroacetate dehalogenase were detected. This suggested that the 2-chloro...
Article
An anaerobic mixed culture enriched over 16 transfers (1/10) from Saale river sediment reductively dehalogenated 1,2,4- and 1,2,3-trichlorodibenzo-p-dioxin (TrCDD) to di- and monochlorinated congeners in the presence of pyruvate (or lactate) and fumarate as cosubstrates. Besides TrCDD, tetrachloroethene and 1,2,3-trichlorobenzene were dechlorinated...
Article
Full-text available
The biodegradation of a mixture of benzene, toluene, ethylbenzene, xylene, (BTEX) and methyl-tert-butyl ether (MTBE) was studied in soil microcosms. Soil inoculation with the toluene-metabolising fungus Cladophialophora sp. strain T1 was evaluated in sterile and non-sterile soil. Induction of biodegradation capacity following BTEX addition was fast...
Article
A bacterium that uses 2-chloroethanol as sole energy and carbon source coupled to denitrification was isolated from 1,2-dichloroethane-contaminated soil. Its 16 S rDNA sequence showed 98% similarity with the type strain of Pseudomonas stutzeri (DSM 5190) and the isolate was tentatively identified as Pseudomonas stutzeri strain JJ. Strain JJ oxidize...
Article
Historic spillages of chlorinated hydrocarbons at a vinyl chloride plant in the Rotterdam Botlek area in The Netherlands have lead to contamination of the underlying aquifer. The principal contaminant is 1,2-dichloroethane (1,2-DCA). The contamination is temporarily contained by a pump-and-treat system. A field trial was carried out to investigate...
Article
Biofouling is a serious problem in industrial recirculating cooling systems. It damages equipment, through biocorrosion, and causes clogging and increased energy consumption, through decreased heat transfer. In this research a fixed-bed biofilter was developed which removed assimilable organic carbon (AOC) from process water, thus limiting the majo...
Article
Full-text available
Uses of a mixture of six volatile fatty acids (VFAs) including acetic, propionic, butyric, isobutyric, valeric and isovaleric acids as electron donors for the reductive dechlorination of chloroethenes have been investigated by both microcosm and column studies. The fates of tetrachloroethene (PCE), cis-dichloroethene (cis-DCE) and vinyl chloride (V...
Chapter
Full-text available
Degradation of chlorinated aromatic and aliphatic organic compounds in natural environments depends on the interplay of a multitude of both abiotic and biotic factors. Hence the extent and rate of these processes can only be understood if, in addition to monitoring the individual microbial processes under the prevailing environmental conditions, de...
Article
Desulfitobacterium strain PCE1 is able to use tetrachloroethene and chloroaromatics as terminal electron acceptors for growth. Cell extracts of Desulfitobacterium strain PCE1 grown with tetrachloroethene as electron acceptor showed no dehalogenase activity with 3-chloro-4-hydroxyphenylacetate (Cl-OH-phenylacetate) and other ortho-chlorophenolic com...
Article
A two-member co-culture consisting of the dehalorespiring Desulfitobacterium frappieri TCE1 and the sulphate-reducing Desulfovibrio sp. strain SULF1 was obtained via anaerobic enrichment from soil contaminated with tetrachloroethene (PCE). In this co-culture, PCE dechlorination to cis-dichloroethene was due to the activity of the dehalorespiring ba...
Article
Full-text available
The dissolved hydrogen concentrations under various redox processes were investigated based on batch experiments. Chloroethenes including tetrachloroethene (PCE), cis-dichloroethene (cis-DCE) and vinylchloride (VC) were respectively used as culture substrates. For each chloroethene, a series of bottles were prepared with the additions of different...
Article
Historic spillages of chlorinated hydrocarbons at a vinyl chloride plant in the Rotterdam–Botlek area in The Netherlands has lead to deep-seated pollution of the underlying aquifer. The principal pollutant is 1,2-dichloroethane (1,2-DCA). As a temporary measure, the contamination is being contained using a pump and treat system. In the long term, i...
Article
Full-text available
Strain TCE1, a strictly anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene (PCE) and trichloroethene (TCE), was isolated by selective enrichment from a PCE-dechlorinating chemostat mixed culture. Strain TCE1 is a gram-positive, motile, curved rod-shaped organism that is 2 to 4 by 0.6 to 0.8 microm and has approximate...
Article
Full-text available
An investigation on the biotransformation of 1,2-dichloroethane (1,2-DCA) in anaerobic groundwater on an industrially polluted site and in the laboratory revealed that reductive, fermentative, and oxidative pathways are involved in the natural attenuation of 1,2-DCA. The stimulation of reductive or oxidative 1,2-DCA transformation in anaerobic aqui...
Article
The plume in the aquifer of an industrial cleaning company's site in Arnhem, the Netherlands, was investigated for redox conditions, and compounds which drive the anaerobic dechlorination of chlorinated aliphatic hydrocarbons (CAH). This study was conducted as the volatile CAH-contaminated site was proven to have a high potential for natural attenu...