Izzet burçin satıcıoğlu

• Doctor of Veterinary Medicine
• Assoc. Prof. at Bursa Uludag University

Three bacterial strains, Mu-43T, Mu-80T, and Mu-86T, were isolated from the 2021 and 2022 mucilage event in the Marmara Sea and were taxonomically characterized. 16S rRNA gene sequence analysis confirmed that these strains belong to the genus Microbacterium. A polyphasic approach involving genomic and phenotypic analysis was employed to determine t...

This study aimed the identification of anaerobic bacteria isolated from blood cultures and the determination of antibacterial susceptibility of the isolates. The study material comprised of 5,282 blood samples taken between 2018 and 2020. The samples were incubated in a BacT/ALERT system. The species identification of the isolates was performed by...

This study explores the biotechnological potential of Planococcus strain S3-L1, isolated from Horseshoe Island in Antarctica during the 6th Turkish Antarctic Expedition in 2022. Marine bacteria inhabiting polar environments play essential roles in ecosystem dynamics and biogeochemical cycles, largely due to their unique adaptations. The Planococcus...

This study employed a One Health approach to assess the potential impact of mucilage on human health by characterizing the prevalence and antimicrobial resistance (AMR) profiles of Escherichia coli and Clostridium perfringens strains isolated during the 2021 mucilage event in the Marmara Sea, Türkiye. Mucilage, a gelatinous organic substance exacer...

Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to Vibrio contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of Vibrio spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-eat stuffed mussels (RTE-SMs) through genome analysis, assessing health risks. The result...

Studies on preventing antimicrobial resistance in aquaculture emphasise the need to responsibly and prudently use antimicrobials, selecting those most effective in controlling and/or reducing mortalities caused by vibriosis. In this study, the distribution of the antimicrobial susceptibility of 28 Vibrio anguillarum isolates from Turkish fish farms...

Campylobacter jejuni is a causative agent of gastroenteritis in humans worldwide, and wild and domestic poultry carry of this bacterium in their gastrointestinal tract. Molecular studies to determine the pathogenicity, origin, and epidemiological relationships among C . jejuni isolates from poultry such as chicken, turkey, and goose consumed as hum...

Our study focused exclusively on analyzing Escherichia coli (E. coli) contamination in fresh raw mussels and ready-to-eat (RTE) stuffed mussels obtained from authorized and regulated facilities. However, it is critical to recognize that such contamination represents a significant public health threat in regions where unauthorized harvesting and sal...

Flavobacterium psychrophilum , a devastating fish pathogen, is responsible for bacterial cold‐water disease (BCWD), also known as rainbow trout fry syndrome. F. psychrophilum is the main causative agent of outbreaks in rainbow trout farms, especially at early live stages. In the present study, we aimed to characterize F. psychrophilum Turkish isola...

Two yellow-coloured strains, F-29 T and F-340 T , were isolated from fish farms in Antalya and Mugla in 2015 and 2017 during surveillance studies. The 16S rRNA gene sequence analysis showed that both strains belong to the genus Flavobacterium . A polyphasic approach involving a comprehensive genome analysis was employed to ascertain the taxonomic p...

This study was aimed at the comparison of three different methods used for the identification of anaerobic bacteria isolated from blood cultures and the determination of the antibacterial susceptibilities of the isolates. The study material comprised of 5282 blood samples. The samples were incubated in a BacT/ALERT system. The species identificatio...

Background: The ever-increasing popularity of home aquariums, most often involving freshwater varieties, has exploded in recent years partially due to the Coronavirus pandemic and related to stay-at-home public health precautions for social distancing. With this ever-increasing popularity of aquariums as a hobby, and whether this involves freshwate...

Outbreaks of bacterial infections in aquaculture have emerged as significant threats to the sustainable production of rainbow trout ( Oncorhynchus mykiss ) worldwide. Understanding the dynamics of these outbreaks and the bacteria involved is crucial for implementing effective management strategies. This comprehensive review presents an update on ou...

The vulnerability of cownose rays (Rhinoptera bonasus) to anthropogenic pressures, including overfishing and habitat degradation, underscores the urgent need for comprehensive conservation efforts to safeguard this species and maintain the ecological equilibrium of marine ecosystems. Still, the susceptibility of cownose rays to Vibrio bacteria unde...

Helicobacter pylori is a pathogen associated with gastroduodenal diseases. This study aimed; (i) to investigate H. pylori presence by invasive tests in adult dyspeptic patients, (ii) to determine antibiotic susceptibility and genotypic characteristics of the H. pylori isolates, and (iii) to investigate the relationship between the H. pylori genotyp...

Brucellosis is a chronic disease caused by Brucella species with a wide range of hosts, from marine mammals to terrestrial species, but with strict host preferences. With the zoonotic character, the prevalence of human brucellosis cases is a reflection of animal infections. This study aimed to identify 192 Brucella isolates obtained from various so...

Epidemiological cut-off value (ECV) analysis for commonly used antimicrobials in aquaculture have not been established for many aquatic pathogens, including Pseudomonas. This study was the first to examine the categorization of 92 aquatic Pseudomonas isolates by calculating seven antimicrobials ECVs using two analytical methods: normalized resistan...

Bacteria of the genus Flavobacterium are recovered from a large variety of environments. Among the described species, Flavobacterium psychrophilum and Flavobacterium columnare cause considerable losses in fish farms. Alongside these well-known fish-pathogenic species, isolates belonging to the same genus recovered from diseased or apparently health...

Plesiomonas shigelloides is the only species in its genus, and has zoonotic importance due to the serious implications resulting from the consumption of contaminated seafood. This is the first report on the genomic features of the whole-genome sequence (WGS) of P. shigelloides strain V-78, recovered from diseased rainbow trout, Oncorhynchus mykiss....

Plesiomonas shigelloides is the only species in the genus and has zoonotic importance due to its serious implications resulting from the consumption of contaminated seafood. This is the first report on the genomic features of the whole-genome sequence (WGS) of P. shigelloides strain V-78 recovered from diseased rainbow trout, Oncorhynchus mykiss. T...

This study was conducted to determine the overall genetic diversity, as well as prevalence and mechanisms of resistance to quinolone antibiotics of 178 Campylobacter jejuni isolated from humans, cattle, dogs, and chickens in Turkey. Multilocus sequence typing (MLST) and E-test were performed for genotyping and antimicrobial susceptibility testing,...

The strain M-43T was isolated from the Oncorhynchus mykiss from a fish farm in Mugla, Turkey. Pairwise 16S rRNA gene sequence analysis was used to identify strain M-43T. The strain was a member of the genus Myroides sharing the highest 16S rRNA gene sequence identity levels of 98.7%, 98.3%, and 98.3% with the type strains of M. profundi D25T, M. od...

The cownose ray, Rhinoptera bonasus, is currently listed as vulnerable and threatened species by the IUCN Red List due to overfishing and bycatch in worldwide resulted declines of the steep population declines of 30-49% in only 43 years. We aimed to present suitable dissection methods for a necropsy of cownose ray with a large, rhomboid-shaped wing...

Four strains, designated as C-2, C-17T, C-39T and Ch-15, were isolated from farmed rainbow trout samples showing clinical signs during an investigation for a fish-health screening study. The pairwise 16S rRNA gene sequence analysis showed that strain C-17T shared the highest identity level of 98.1% with the type strain of Chryseobacterium piscium L...

Bacteria of the genus Flavobacterium are recovered from a large variety of environments. Among the described species, Flavobacterium psychrophilum and Flavobacterium columnare are causing considerable losses in fish farms. Alongside these well-known fish-pathogenic species, isolates belonging to the same genus recovered from diseased or apparently...

The genus Vibrio includes important pathogenic species for human and aquatic organisms such as Vibrio cholera, V. parahaemolyticus, V. vulnificus, V. anguillarum or V. harveyi. At present, Vibrionaceae family consists of >190 described species, classified into nine genera. Vibrio are widespread in shellfish, finfish and marine ecosystems and show r...

A strain, S-1T was isolated from rainbow trout (Oncorhynchus mykiss) exhibiting clinical symptoms of lens atrophy, inappetence, visual impairment and growth retardation. The strain was identified as representing a member of the genus Shewanella on the basis of the results of 16S rRNA gene sequence analysis. The neighbor-joining phylogenetic tree ba...

The isolation of Pseudomonas haemolytica from different habitats as well as its distribution over a wide geographical area, possible reservoir role for antibiotic resistance and zoonotic potential, all require the detailed characterization of P. haemolytica strains. In the present study, we describe 18 phenotypically similar strains isolated from t...

In the evolving biosphere, pathogenic microorganisms that cause disease may undergo phenotypic changes. While some of these changes result in new variants or mutants, others lead to the emergence of novel pathogens. Such phenotypic changes as well as advances in technology and analytical methods, and the identification of genomic sequences of micro...

Helicobacter pylori is a Gram-negative bacterium that colonizes approximately half of the world's population and is associated with chronic gastritis, peptic ulcer, and gastric malignancy. 1 The genotypes of H. pylori strains isolated from human gastric mucosa in different localizations of the world show phylogeographic differentiation. Therefore,...

Eleven Gram-negative, curved and S-shaped, oxidase activity positive, catalase activity negative bacterial isolates recovered from faeces of Anatolian ground squirrel ( Spermophilus xanthoprymnus ) in the city of Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR revealed that these isolates belonged to...

A total of 26 Gram-negative, motile, gently curved, and rod-shaped isolates were recovered, during a study to determine the faeco-prevalence of Helicobacter spp. in urban wild birds. Pairwise comparisons of the 16S rRNA gene sequences indicated that these isolates belonged to the genus Helicobacter and phylogenetic analysis based on the 16S rRNA ge...

In the present study, we sampled sea bream and sea bass, weighing 400-450g, for routine microbiological, hematological, macroscopical and histopathological examinations. Samples were taken from seemingly healthy fish, which displayed increased feed conversion rates, signs of cold shock and slow movement. Routine bacteriological analyses included ba...

Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to characterize Bacillus anthracis isolates recovered from a variety of hosts and environment...

During a study aiming at isolation and genomic characterization of non-pathogenic fish symbionts, strain F-328T was isolated from skin mucus of healthy-seeming rainbow trout (Oncorhynchus mykiss) and subjected to polyphasic characterization, including a comprehensive genome analysis. Strain F-328T was Gram stain negative, none-gliding, strictly aer...

In this study, it was aimed to present the results of microbiological, cytological, histopathological, and immunohistochemical analyses of ocular samples from an Antarctic (Ardley Island, King George Island) Gentoo penguin chick (Pygoscelis papua) with a pyogranulomatous lesion in the right eye. Samples were taken from both the healthy left eye and...

Seventy-four Gram-negative, motile, slightly curved rod-shaped, microaerophilic, oxidase-positive and catalase-negative isolates, recovered from fecal samples of the Anatolian ground squirrel (Spermophilus xanthoprymnus) in Kayseri, Turkey, were subjected to a polyphasic taxonomic study. Results of a genus-specific PCR indicated that all isolates b...

Two yellow-pigmented isolates, F-60T and F-392, were isolated from the internal organs of an apparently healthy rainbow trout (Oncorhynchus mykiss). The strains were identified as members of the genus Flavobacterium based on the results of 16S rRNA gene sequence analysis. Strains F-60T and F-392 had the highest 16S rRNA gene sequence identity level...

Cells of strains P66T , V1 and W15Feb18 are Gram-stain-negative short rods and motile by one polar flagellum. Strain P66T was isolated from rainbow trout (Oncorhynchus mykiss) cultivated at a fish farm in Turkey. Strain V1 was isolated from sand of an intertidal shore on the Galicia coast in Spain and strain W15Feb18 was isolated from water collect...

Streptococcus species are of major concern for both warm-water and cold-water salmonid species, and have zoonotic importance. While Lactococcus garvieae, associated with warm-water streptococcosis, causes thousands of tons of mortalities, Vagococcus salmoninarum, the causative agent of cold-water streptococcosis, leads to chronic disease and devast...

Identification of novel Flavobacterium species from fish samples has growing importance due to their notorious effects on freshwater fish species. During the course of studying farmed rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) samples exhibiting clinical signs in Rize, Turkey, two isolates were recovered, and their taxonomic positions were...

In the present study, we describe a pathogenic incidence of Shewanella sp. S-1 infection in cultured rainbow trout (Oncorhynchus mykiss, Walbaum, 1792), as evidenced by lens atrophy, inappetence, visual impairment, and growth retardation. Strain S-1 was identified using basic microbiological tests, the BD Phoenix™ rapid diagnostic kit, the MALDI-TO...

In a study carried out between 2013-2018 in fish farms in Turkey, several putative novel species were isolated. The 16S rRNA nucleotide sequences of fourteen strains of Gram-negative rods, which were isolated from asymptomatic and symptomatic rainbow trouts (Onchorhynchus mykiss), placed them under the genus Pseudomonas. The similarity values of th...

Six Gram negative, motile bacteria were isolated from rainbow trout ( Oncorhynchus mykiss ). The 16S rRNA sequence similarity values grouped them in the Pseudomonas mandelii (strains P49, P50 T , 154a T and P154b), Pseudomonas fluorescens (strain P115 T ) and Pseudomonas koreensis (strain P155 T ) phylogenetic subgroups in the genus Pseudomonas . T...

During a study on culturable microorganisms from fish farms, four yellow-pigmented gram negative, rod shaped isolates, F-47T, F-339T, F-380 and F-400, were recovered from rainbow trout samples exhibiting clinical signs. Based on 16S rRNA gene sequence analysis, the strains were identified as members of the genus Flavobacterium. Strains F-47T and F-...

In this study, it was aimed to present the results of microbiological, cytological, histopathological, and immunohistochemical analyses of ocular samples from an Antarctic (Ardley Island, King George Island) Gentoo penguin chick ( Pygoscelis papua ) with pyogranulomatous panophthalmitis in the right eye. Samples were taken from both the healthy lef...

The prevalence of Pseudomonas species in freshwater fish and water from aquaculture farms in Turkey was determined on a monthly basis, in the period covering 2013–2017 and two seasons of 2018. The farms included in the study were located in six different regions of Turkey. A total of 90 Pseudomonas strains were isolated from water and diseased and...

Fish diseases caused by bacterial genera belonging to the family Flavobacteriaceae, especially Tenacibaculum, Flavobacterium, and Chryseobacterium, are responsible for losses in wild and farmed fish around the world. In the last decade, the genus Chryseobacterium has rapidly grown in parallel with numerous novel Chryseobacterium species described f...

In recent years, species in the Chryseobacterium genus have emerged as opportunistic fish pathogens that can cause death in fish in many countries. In the last decade, C. aahli, C. oncorhynchi, C. chaponense, and C. piscico-la have been reported to cause systemic infections in fish. In the present study, Chryseobacterium sp. C-204 was iso-lated fro...

Modern aquaculture enables effective means for intensive aquaculture production under “controllable” conditions. This rapidly growing industry, however, has experienced relatively severe disease problems owing to a lack of control of the microbiota in rearing systems. In the present study, we identified the opportunistic pathogens and some members...

Numerous Pseudomonas species can cause diseases in humans, animals, and aquatic organisms. Pseudomonas species have been considered one of the most common bacterial infectious agents among cultured fish species and are considered to cause stress-related diseases of freshwater fishes, especially under culture conditions. In this study, Pseudomonas s...

Twelve isolates recovered from rainbow trout weighing 1-4 grams showing signs of anorexia, exophthalmos, darkening, and dorsal fin erosion were used in our study. In addition to conventional microbiological tests, comprehensive phenotypic characterization has been performed using the Biolog GEN III microplate. Molecular identification and character...

Acinetobacter species are ubiquitous and can be found in different environmental sources such as hydrocarbon contaminated areas, activated sludge, sewage, and vegetables, animals, and humans. Members belonging to the genera Acinetobacter has also been found to be dominant in the intestinal microbiota of fish. During the last decade, some genus memb...

Pseudomonas es el género que presenta el mayor número de especies dentro de las bacterias Gram negativas. Se distinguen varios grupos filogenéticos, siendo el de Pseudomonas fluorescens el mayoritario. Se ha realizado un estudio de la presencia de especies del género Pseudomonas en centros de acuicultura de Turquía entre los años 2013 a 2018. Se ha...

Aim: Members of the genus Chryseobacterium have emerged as opportunistic fish pathogens on multiple continents. During the last ten years alone, numerous novel Chryseobacterium spp. was described and recovered from systemically infected fishes exhibiting clinical disease signs worldwide. In this study, C-2 isolate was recovered from rainbow trout...

The present study was aimed at determining antimicrobial susceptibility by a CLSI standard microdilution testing protocol and detecting the resistance genes of motile Aeromonas species isolated from cultured fish. The importance of the minimum inhibitory concentrations was assessed based on statistically determined epidemiological cut‐off values ca...

A study of 91 isolates from fish farms in Turkey showed that isolates P7T, P11, P24b, P29, P72, P73 and P158 belonged to the genus Pseudomonas according to 16S rRNA nucleotide sequence analysis. The analysis of the sequences of the RNA polymerase sigma factor gene (rpoD) located these strains in the Pseudomonas fluorescens lineage of species within...

Bacterial agents must be genotypically analyzed for vaccinations, effective control programs, and antimicrobial resistance genes that could transfer from aquaculture settings to terrestrial ecosystems and humans. Therefore, we investigated the prevalence, genotypic characterization, and antimicrobial resistance of Lactococcus garvieae for two years...

Introduction: The aquatic environment can serve both as a natural reservoir of antibiotic resistance genes and the spreading of bacteria and antimicrobial resistance genes to environment. Plasmid-mediated quinolone resistance (PMQR) genes represent an important challenge to the effectiveness of quinolones in the treatment of human and animal infect...

Introduction: The genus Pseudomonas is one of the most complex Gram-negative bacterial genera as it currently is the genus with the largest number of species. While Pseudomonas cause severe disease for humans, the incidence or transmission route not known very well from aquaculture to humans. Recently, there are many possible novel Pseudomonas spec...

The antimicrobial susceptibilities of Turkish F. psychrophilum isolates were investigated using a Clinical and Laboratory Standards Institute (CLSI) standard microdilution testing protocol. The significance of the minimum inhibitory concentrations was determined by applying empirically and statistically determined epidemiological cut-off values cal...

The rainbow trout production increased more than 100% in the last decade and total rainbow trout production was shown as 107.013 tons according to 2016 statics. According to Federation of European Aquaculture Producer, Turkey was determined as the biggest rainbow trout producer into the European Countries in 2016. Together with high production capa...

Turkey was the largest rainbow trout producer of the European countries in 20161, and the reason for this production is mainly attributed to its egg and fry production. Flavobacterium psychrophilum cause the highest rates of mortality in the starting to feeding stages of the fish2. Diversity among F. psychrophilum isolates was reported at different...

Flavobacterial diseases in fish are caused by multiple bacterial species within the family Flavobacteriaceae and are responsible for devastating losses in wild and farmed fish stocks around the world. With the recent advances in molecular biology, several novel genera within the family Flavobacteriaceae (e.g., Chryseobacterium, Elizabethkingia and...

Bu çalışmada, gökkuşağı alabalıklarından izole edilen altı L. anguillarum izolatı ve bir referans suş fenotipik ve genotipik özellikler açısından incelendi. İzolatların fenotipik özelliklerinin belirlenmesinde klasik mikrobiyolojik testler ve API 20 E strip testi kullanıldı. İzolatların moleküler identifikasyonu amiB genine dayanan L. anguillarum t...

Fecal contaminants are a major public concern that directly affect human health in the fish production industry. In this study, we aimed to determine the fecal coliform, spoilage bacteria, and antimicrobial-resistant bacterial contamination in rainbow trout (Oncorhynchus mykiss) farms. Fish were sampled from rainbow trout farms that have a high pro...

In the field of fish physiology, the species‐specific parameters of blood, biochemistry and hormones are especially unknown. The main reason for this are difficulties of blood drawing from some fish species and non‐standardized blood reference values that change for fish weight, water temperature and other environmental variables. Information and i...

Aim: The identification, prevalence and phylogenetic relationships of motile Aeromonas spp. which isolated from different fish and might be pathogenic species for fish were aimed in the present study. Materials and Methods: A total of 99 motile Aeromonas isolates and 3 reference species (ATCC) isolated from different water temperatures, different m...

Aim: The main objective of the rainbow trout (Oncorhynchus mykiss Walbaum, 1792) is to carry interest by ensuring that the fish they cultured are as close to market weight as possible. In this context, the production of the cultured fish, which first started in 1980s in our country, showed a rapid development in the last fifteen years and today it...

Turkey was the largest rainbow trout producer of the European countries in 2016, and the reason for this production is mainly attributed to its egg and fry production. Flavobacterium psychrophilum cause the highest rates of mortality in the starting to feeding stages of the fish. In the present study, twenty‐five F. psychrophilum isolates recovered...

The aims of this study were to determine the prevalence and phylogenetic relationship of motile Aeromonas spp. that might be pathogenic species for rainbow trout in infected/mix infection cases (based upon different outbreaks on fish farms). A total of 99 motile Aeromonas isolates (and three reference strains) were analysed that were isolated from...

Abstract Fish farms are established generally on the stream or dam lakes regardless of if the water supply is mixed with waste water, urban water, and other farm water. Also there is the most important factor is other terrestrial animal could use this water or contaminated it. Aquaculture waters could be polluted when urban wastes mixing of farm wa...

Aquaculture has become an important candidate as an animal protein source through its growth over the last decade. Based upon a report from the Food and Agriculture Organization of the United Nations, it is the fastest growing sector of the food industry, yet the pathogenicity of many biological agents involved in aquaculture is still unknown. In t...

Aquaculture has growing much more than 100% at the last decade. Rainbow trout is the most economically important fish among the aquatic species. Bacterial agent, especially, Yersinia ruckeri and Lactococcus garvieae have limited freshwater fish production all over the world. There are many reports about identification of bacterial pathogens in aqua...

Motile Aeromonads isolate were identified with gyrB gene region and all gene were accepted in GenBank database A. sobria, A. salmonicida, A. media, Aeromonas sp., A. bestiarum, A. veronii, A. allosaccharophila, A. hydrophila, A. eucrenophila, A. hydrophila subsp. hydrophila, A. hydrophila subsp. anaerogenes, A. dhakensis, A. hydrophila subsp. dhake...

ABSTRACT: In this study, we compared 142 Yersinia ruckeri isolates collected between 2013 and 2016 from 6 different regions of Turkey. A total of 18 different genogroups were found, though most of the isolates clustered into the same genogroup as serotype O1. As immunization of fish with inactivated Y. ruckeri by injection, immersion or feeding pro...

Infectious pancreatic necrosis virus (IPNV) is a common pathogen of rainbow trout (Oncorhynchus mykiss). In this report, the prevalence of IPNV in rainbow trout farms in Turkey was investigated. Sequence and phylogenetic analyses of VP2 from 30 viruses from rainbow trout, one from a local salmonid species (Salmo trutta macrostigma) and one from com...