How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
Izuddin Rahman currently works at the Department of Biotechnology, Institute for Agricultural Research and Development Malaysia. Izuddin does research in Biotechnology.
The use of enzymes in detergent formulations is becoming popular due to the concerns about the environment. T1 lipase (E.C. 18.104.22.168) was evaluated for its stability and performance in dishwashing along with other common components of an automatic dishwashing detergent. Therefore, the process of formulating the detergent would depend on the stabilit...
I performed Tricine SDS PAGE according to the Nature protocol. Included in the gel is 4% stacking, 10% spacer, and 16% resolving. I intended to separate peptides lower than 10kDa. Therefore, I used the Vivaspin 10kDa membrane filter. I loaded around 30 ug of protein. However, there were smaller than 10kDa bands appear within the retentate of the 10kDa filter. How is this possible and how to resolve this? Attached is the photo. Thank you in advance everyone.