Izabela Sadowska-Bartosz

Izabela Sadowska-Bartosz
Rzeszów University · Laboratory of Analytical Biochemistry

Professor

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101
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Publications

Publications (101)
Chapter
The increasingly aged human population (mainly in developed countries) represents a significant scientific achievement and privilege associated with medical, social, and economic progress. However, it also poses several challenges to national health and social care systems. The uncoupling of biological evolution with the vast and fast technical pro...
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There is recent interest in a diet that can be recommended for patients suffering from cancer. In this respect, the effects were studied of the extracts of several common fruits, herbs and vegetables on the viability of two human ovary cancer cell lines (SKOV-3 and PEO1) in vitro. Normal human MRC-5 fibroblasts were used as a control cell line. The...
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A modification of the ABTS• decolorization assay for plate readers is presented. In our modification, 200 µL of ABTS solution of absorbance 1.0 at 734 nm was added with an antioxidant and decreased absorbance resulted. For comparison of antioxidant activities in the kinetic assay of absorbance decrease, concentration dependence of absorbance decrea...
Article
Anthocyanins are powerful antioxidants with numerous beneficial health effects. However, their autoxidation may produce hydrogen peroxide. Black carrots owe their colour to the high anthocyanin content. The aim of this study was to compare the antioxidant capacity of and hydrogen generation by black and orange carrots. Black carrots were found to h...
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Hydrogen peroxide (H2O2) is generated under autoxidation of some components of beverages including flavonoids and ascorbate, especially in tea and coffee. As polyphenols are also present in solid food, especially in vegetables, we checked whether hydrogen peroxide is generated during cooking of several common vegetables. The formation of hydrogen p...
Article
There are conflicting reports on the antioxidant activity of hispidulin. Antioxidant activity of hispidulin was evaluated using assays of ABTS• reduction, ferric ion reducing antioxidant power (FRAP) assay, DPPH reduction assay, and protection of erythrocyte membranes against lipid peroxidation and protein thiol oxidation. ABTS• reduction assay poi...
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It has been argued that the mol/cell metric is more universal than concentration of the toxic agent since in many cases the effect of dose expressed as mol/cell is independent of ex-perimental setup. We confirmed it for hemolysis of erythrocytes in phosphate-buffered saline induced by hypochlorite where the amount of femtomoles/cell of hypochlorite...
Article
Wines contain significant amounts of polyphenols which autoxidize upon contact with oxygen, producing hydrogen peroxide. We checked more than 40 samples of white and red wines finding, on average, production of small amounts of hydrogen peroxide within 3 h after the wines had contacted with air, though some samples did not produce detectable amount...
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Phytoestrogens are naturally occurring non-steroidal phenolic plant compounds. Their structure is similar to 17-β-estradiol, the main female sex hormone. This review offers a concise summary of the current literature on several potential health benefits of phytoestrogens, mainly their neuroprotective effect. Phytoestrogens lower the risk of menopau...
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Betalains are water-soluble pigments present in vacuoles of plants of the order Caryophyllales and in mushrooms of the genera Amanita, Hygrocybe and Hygrophorus. Betalamic acid is a constituent of all betalains. The type of betalamic acid substituent determines the class of betalains. The betacyanins (reddish to violet) contain a cyclo-3,4-dihydrox...
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The damage to SH-SY5Y cells by 6-hydroxydopamine (6-OHDA) is an established cellular model of Parkinson’s disease (PD). Redox nanoparticles are a promising tool for therapy, including neurodegenerative diseases. As pH of the brain tissue at sites affected by PD is lowered down to 6.5, we studied the effect of pH-responsive redox nanoparticles (poly...
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3-Bromopyruvic acid (3-BP) is a promising anticancer compound. Two ovary cancer (OC) cell lines, PEO1 and SKOV3, showed relatively high sensitivity to 3-BP (half maximal inhibitory concentration (IC50) of 18.7 and 40.5 µM, respectively). However, the further sensitization of OC cells to 3-BP would be desirable. Delphinidin (D) has been reported to...
Article
We found that the oxidation of cyanidin with hydrogen peroxide, tert-butyl hydroperoxide, 2,2'-azobis(2-methylpropionamidine), 3-morpholinosydnonimine hydrochloride, sodium hypochlorite, Fe3+, and Fe2+ induced the appearance of a new fluorescence band, centered at 525-540 nm when excited at 470-490 nm. The intensity of this fluorescence was related...
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Due to the progressive aging of the society, the prevalence and socioeconomic burden of neurodegenerative diseases are predicted to rise. The most common neurodegenerative disorders nowadays, such as Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis, can be classified as proteinopathies. They can be either synucleinopathie...
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Replicative senescence is an unalterable growth arrest of primary cells in the culture system. It has been reported that aging in vivo is related to the limited replicative capacity that normal somatic cells show in vitro. If oxidative damage contributes to the lifespan limitation, antioxidants are expected to extend the replicative lifespan of fib...
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The inhibitory effects a range of synthetic and natural antioxidants on lipid peroxidation of egg yolk and erythrocyte membranes induced by a free radical generator 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) was compared, with significant differences being found between both systems. When the protection by selected antioxidants against th...
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Parkinson’s disease (PD) patients can benefit from antioxidant supplementation, and new efficient antioxidants are needed. The aim of this study was to evaluate the protective effect of selected nitroxide-containing redox nanoparticles (NRNPs) in a cellular model of PD. Antioxidant properties of NRNPs were studied in cell-free systems by protection...
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The aim of this study was to characterize the interaction of chosen catechins ((+)-catechin, (-)-epigallocatechin (EGC), and (-)-epigallocatechin gallate (EGCG)) with human erythrocytes and their protective effects against oxidative damage of erythrocytes. Uptake of the catechins by erythrocytes was studied by fluorimetry, their interaction with er...
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Nitroxides and nitroxide-containing nanoparticles (RNP) are excellent antioxidants. However, they have relatively high reduction potentials, which make them behave like oxidants or show little activity in some antioxidant assays. We found that stable nitroxyl radicals (TEMPO and 4-amino-TEMPO) has low reactivity in the test of scavenging of 2,2′-az...
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The study was aimed at evaluation of the role of secondary oxidative stress in the stress-induced premature senescence (SIPS) of human fibroblasts induced by H2O2. Two fibroblast lines were used: lung MRC-5 and ear H8F2p25LM fibroblasts. The lines differed considerably in sensitivity to H2O2 (IC50 of 528 and 33.5 μM, respectively). The cells were e...
Article
Aims: 3-Bromopyruvate (3-BP), an alkylating agent and a glycolytic inhibitor, is a promising anticancer agent, which can be efficient also against multidrug-resistant cancer cells. The aim of this study was to examine how 3-BP affects the survival and mobility of rat (MAT-LyLu and AT-2) and human (DU-145 and PC-3) metastatic prostate cancer cell l...
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Aims The aim of the study was to check whether two flavanols [(‐)‐epigallocatechin gallate and (+)‐catechin] can ameliorate oxidative stress accompanying and contributing to the lethal effects of heating (50°C) and freezing‐thawing on the yeast Saccharomyces cerevisiae. Methods and results The flavanols studied increased yeast survival during heat...
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Abstract Oxidative stress (OS) and nitrative stress (NS) accompany many diseases, including Alzheimer’s disease (AD) and Parkinson’s disease (PD). Antioxidants have been proposed to counteract OS/NS in these diseases. Nevertheless, the effects of antioxidants are limited and new, more efficient antioxidants are searched for. Redox-active nanopartic...
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Studies of 54 antioxidants revealed that 27 of them, mainly polyphenols, generated hydrogen peroxide (H2O2) when added to Dulbecco’s modified Eagle’s medium (DMEM), other media used for culture of mammalian and yeast cells and phosphate-buffered saline. The most active antioxidants were: propyl gallate (PG), (−)-epigallocatechin gallate (EGCG) and...
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An alkylating compound, 3-bromopyruvic acid (3-3-bromopyruvic acid (BP)) is a promising anticancer agent, potentially able to act on multidrug-resistant cells. Its action has been attributed mainly to inhibition of glycolysis. This compound induces also oxidative stress at a cellular level. The effects of 3-BP on gene expression have not been studi...
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Undesired modifications of biomolecules (proteins, lipids and nucleic acids), mainly oxidation by reactive oxygen species, underlay many diseases and apparently aging. Compounds counteracting these modifications can therefore be candidates for potential drugs and aging-modifying agents. Naturally occurring compounds are especially interesting in th...
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Interaction of metal, especially iron ions with flavanols is considered as an important feature of these compounds and is believed to contribute to their both antioxidant and prooxidant properties. The aim of this study was to examine how Fe²⁺ binding to form a 4:1 (flavanol:Fe²⁺) mixtures affects the antioxidant properties of flavanols. ABTS∗ scav...
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Non-enzymatic protein modifications occur inevitably in all living systems. Products of such modifications accumulate during aging of cells and organisms and may contribute to their age-related functional deterioration. This review presents the formation of irreversible protein modifications such as carbonylation, nitration and chlorination, modifi...
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Interaction of metal, especially iron ions with catechins is considered as an important feature of these compounds and is believed to contribute to their both antioxidant and prooxidant properties. The aim of this study was to examine how Fe²⁺binding to form a 1:1 complex affects the antioxidant properties of catechins (flavan-3-ols). The following...
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3-bromopyruvic acid (3-BP) is a promising anticancer agent, potentially able to act on multidrug-resistant cells. Its action has been attributed mainly to inhibition of glycolysis. This compound induces also oxidative stress at a cellular level. The effects of 3-BP on gene expression have not been studied, although they may determine the survival o...
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Catechins (Flavan-3-ols), the main components of tea extracts, rise increasing interest due to their biological, mainly antioxidant, effects. The present study aims to investigate the capacity of selected catechins [(−)-Epigallocatechin gallate and (+)-Catechin] to protect Saccharomyces cerevisiae (two strains: wild type BY4741 and mutant BY4741Δso...
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Salivary gland tumors account for 3–6% of tumors of the head and neck. About 80% of salivary gland tumors occur in parotid glands. Oxidative stress (OS) is implicated in the origin, development, and whole-body effects of various tumors. There are no data on the occurrence of OS in the parotid gland tumors. The aim of this study was to ascertain if...
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Ribonucleotide reductase (RNR) catalyses the rate limiting step of DNA synthesis utilising a mechanism that requires a tyrosyl radical. We have previously shown that superoxide can quench protein tyrosyl radicals in vitro, either by oxidative addition, or reduction of the radical to tyrosine. Here, we observe that Saccharomyces cerevisiae strains l...
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TEMPO-phosphate has been introduced as a phosphate analogue to study phosphate transport in erythrocytes. The nitroxide is reduced intracellularly upon entering the cells, the membrane transport being the rate-limiting step of the loss of ESR signal. The use of TEMPO-phosphate is convenient and avoids the hazard of radioactivity. We studied the inh...
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Multiple sclerosis (MS) is a disease involving oxidative stress (OS). This study was aimed at examination of the effect of melatonin supplementation on OS parameters, especially oxidative protein modifications of blood serum proteins, in MS patients. The study included 11 control subjects, 14 de novo diagnosed MS patients with the relapsing-remitti...
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Antioxidant properties of five catechins and five other flavonoids were compared with several other natural and synthetic compounds and related to glutathione and ascorbate as key endogenous antioxidants in several in vitro tests and assays involving erythrocytes. Catechins showed the highest ABTS-scavenging capacity, the highest stoichiometry of F...
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Main thiols and disulfides were determined in the hemolymph of the Jamaican field cricket Gryllus assimilis at various developmental stages. On the basis of these data, redox potentials of the glutathione, cysteine and homocysteine redox systems were calculated. The concentrations of all thiols studied decreased during development (at a stage of 6...
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Myasthenia gravis (MG) is an autoimmune disease caused by production of antibodies against acetylcholine receptors of the neuromuscular junction (Ab). The aim of this study was to ascertain if oxidative stress accompanies MG by estimation of the several independent parameters of oxidative damage, mainly the levels of oxidative modifications of bloo...
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Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe(2+) complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe(2+) in the Fenton reac...
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Myasthenia gravis (MG) is an autoimmune disease caused by production of antibodies against acetylocholine receptors of the neuromuscular junction (Ab). As oxidative damage is involved in inflammatory and autoimmune-mediated diseases, we studied oxidative modifications of blood plasma proteins in patients with MG. The group studied consisted of 50 M...
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Vast evidence supports the view that glycation of proteins is one of the main factors contributing to aging and is an important element of etiopathology of age-related diseases, especially type 2 diabetes mellitus, cataract and neurodegenerative diseases. Counteracting glycation can therefore be a means of increasing both the lifespan and healthspa...
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Aims: Cancer cells, due to the Warburg effect, are more dependent on glycolysis than normal cells, so glycolytic inhibitor 3-bromopyruvic acid (3-BP) was proposed as a promising candidate for anticancer therapy. Overexpression of multidrug transporters is the main reason of resistance of cancer cells to chemotherapy. As the activity of multidrug t...
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The aim of this study was to compare the antioxidant activities of six atypical antipsychotic drugs: clozapine (CLZ), quetiapine, olanzapine (OLA), risperidone, ziprasidone, aripiprazole (ARI), as well as a typical antipsychotic drug, haloperidol. Several tests of antioxidant activity were used: protection of thiol groups against oxidation by perox...
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Background: 3-Bromopyruvic acid (3-BP), a glycolytic inhibitor and a promising anticancer compound, induces oxidative stress and depletes cells of glutathione (GSH). The causes of GSH loss remain unclear. The aim of this study was to ascertain whether 3-BP forms a conjugate with glutathione. Methods: GSH was incubated with various amounts of 3-B...
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Glycation is the cause of diabetic complications and contributes to the development of other diseases and aging. Numerous exogenous compounds have been tested for their anti-glycating activity. The aim of this study was to answer the question, which endogenous compounds at physiological concentrations can effectively prevent glycation. A set of end...
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Novel approaches to cancer chemotherapy employ metabolic differences between normal and tumor cells, including the high dependence of cancer cells on glycolysis ("Warburg effect"). 3-Bromopyruvate (3-BP), inhibitor of glycolysis, belongs to anticancer drugs basing on this principle. 3-BP was tested for its capacity to kill human non-invasive MCF-7...
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One of the main mechanisms of etiopathogenesis of diabetes and diabetic complications is an increase in the level of glycation of proteins due to increased concentrations of glucose and other reactive metabolites. The level of protein glycation is elevated also in other diseases such as multiple sclerosis or cystic fibrosis [1]. The search for new...
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One of the main mechanisms of etiopathogenesis of diabetes and diabetic complications is an increase in the level of glycation of proteins due to increased concentrations of glucose and other reactive metabolites. The level of protein glycation is elevated also in other diseases such as multiple sclerosis or cystic fibrosis [1]. The search for new...
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Numerous data point to the occurrence of oxidative stress in cystic fibrosis, which is conditioned by both increased generation of reactive oxygen species, especially in the lung due to inflammation and to decreased levels of antioxidants, especially fat-soluble vitamins, caused by malabsorption. To compensate for the lack of antioxidants, various...
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Nitroxides are promising compounds for prevention of undesired protein modifications. The aim of this study was to compare the efficiency of 11 nitroxides, derivatives of 2,2,6,6-tetramethylpiperidine-1-oxide (TEMPO) and 2,2,5,5-tetramethylpirrolidine-1-oxyl (PROXYL) in prevention of nitration and oxidation of model compounds and human serum albumi...
Chapter
Oxidative, nitrosative/nitrative, and chlorinative stress, due to overproduction of reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive chlorine species (RCS), respectively, inflicts damage to all classes of biomolecules – lipids, proteins, nucleic acids, and carbohydrates – and depletes low-molecular-weight antioxidants. T...
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Peroxynitrite, formed in vivo in a reaction between nitric oxide and superoxide radical anion, is a compound of profound pathophysiological importance. The formation of peroxynitrite is intensified in many diseases, including neurodegenerative ones, and its reactions appear to contribute to the development of these diseases. Therefore, prevention o...
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Peroxynitrite anion (ONOO - ) is a potent oxidizing and nitrating agent. A primary effect of ONOO - on proteins is nitration of tyrosine residues. Nitration of tyrosine residues has been implicated in the pathophysiology of various, particularly neurodegenerative, disorders and aging, therefore search for agents preventing or limiting protein modif...
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The aim of the study was to compare the effects of ascorbic acid (AA) in vitro in the absence and in the presence of cell-dependent recycling. In a cell-free system, AA enhanced glycoxidation of bovine serum albumin (BSA) by glucose and induced BSA glycation in the absence of sugars. On the other hand, AA did not affect erythrocyte hemolysis, glyca...
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The role of metal (especially) iron ions has been postulated to play a prominent role in protein glycation, suggesting antiglycating effectiveness of metal chelators. However, this rule may not apply to all model glycation systems. We found that metal chelators are not effective in prevention of glycation of bovine serum albumin (BSA) in vitro, and...
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Ascorbic acid (AA) has been reported to be both pro-and antiglycating agent. In vitro, mainly proglycating effects of AA have been observed. We studied the glycation of bovine serum albumin (BSA) induced by AA in vitro. BSA glycation was accompanied by oxidative modifications, in agreement with the idea of glycoxidation. Glycation was inhibited by...
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Exposure to high glucose concentrations in vitro is often employed as a model for understanding erythrocyte modifications in diabetes. However, effects of such experiments may be affected by glucose consumption during prolonged incubation and changes of cellular parameters conditioned by impaired energy balance. The aim of this study was to compare...
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Non-enzymatic protein glycosylation (glycation) contributes to many diseases and aging of organisms. It can be expected that inhibition of glycation may prolong the lifespan. The search for inhibitors of glycation, mainly using in vitro models, has identified natural compounds able to prevent glycation, especially polyphenols and other natural anti...
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Abundant evidence points to a significant role of nitric oxide (NO) and the main enzyme producing NO in the central nervous system (NOS-1) in the pathogenesis of schizophrenia. Effects of NO on dopamine release in the striatum and prefrontal cortex have been reported indicating that NO is an important modulator of dopaminergic activity. Further, NO...
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Peroxynitrite is believed to contribute to pathogenesis of various diseases. Search for drugs and nutraceuticals able to limit peroxynitrite reactions is thus of interest. This study aimed to compare the capacity of 16 flavonoids and several other compounds present in food to prevent peroxynitrite reactions in three different test systems, based on...
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Abstract Seven nitroxides of different structure were studied for the ability to prevent glycoxidation of bovine serum albumin incubated with three monosaccharides (glucose, fructose and ribose). Glycoxidation was estimated by fluorimetric parameters of protein modifications (formation of Advanced Glycation Endproducts - AGEs, dityrosine, N'-formyl...
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The aim of this study was to compare the kinetics of the glycoxidation of bovine serum albumin (BSA) as a model protein by three sugars: glucose, fructose and ribose, using fluorometric measurements of the content of advanced glycation end products (AGEs), protein-bound fructosamine, dityrosine, N'-formylkynurenine, kynurenine, tryptophan, the cont...
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Glycoxidation belongs to posttranslational protein modifications which underlie pathological sequelae of diabetes and other diseases, and contribute to aging. Search for efficient inhibitors of glycoxidation is therefore of considerable importance. We studied the effect of various polyphenols on the glycoxidation of bovine serum albumin (90 uM) inc...
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Abstract Proteins are subject to various posttranslational modifications, some of them being undesired from the point of view of metabolic efficiency. Prevention of such modifications is expected to provide new means of therapy of diseases and decelerate the process of aging. In this review, modifications of proteins by reactive nitrogen species an...