Iwona Kwiecień

Iwona Kwiecień
Wojskowy Instytut Medyczny · Laboratory of Flow Cytometry and Hematology, Department of Internal Medicine and Hematology

PhD

About

70
Publications
8,020
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429
Citations
Additional affiliations
February 2017 - present
Wojskowy Instytut Medyczny
Position
  • Specialist
May 2016 - present
Independent Public Central Clinical Hospital, Banacha 1a Warsaw, Poland
Position
  • Research Assistant
September 2012 - September 2016
Medical University of Warsaw
Position
  • PhD

Publications

Publications (70)
Article
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Tobacco smoking remains the main cause of tobacco-dependent diseases like lung cancer, chronic obstructive pulmonary disease (COPD), in addition to cardiovascular diseases and other cancers. Whilst the majority of smokers will not develop either COPD or lung cancer, they are closely related diseases, occurring as co-morbidities at a higher rate tha...
Article
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Lymphocytes play a leading role in regulation of the immune system in lung cancer patients. The recognition of T cells profile may help in prediction of effectiveness of anticancer immunotherapy. The aim of the study was to determine the dominant subpopulation of CD4+ and CD8+ lymphocytes in metastatic and non-metastatic lymph nodes (LNs) of lung c...
Article
Monocytes play a role in viral biology, but little is known about the monocyte subpopulation in the course of COVID-19 disease. The aim of the study was the analysis of classical, intermediate and non-classical monocytes with expression of PD-L1 and CD62L, TIM-3 and CD86 molecules in peripheral blood (PB) to distinguish patients with SARS-CoV-2 inf...
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The evaluation of argyrophilic nucleolar organizer regions (AgNORs) uses a simple method used in research into neoplasm. Bone marrow aspirates from 70 patients with acute leukemia underwent morphological, immunophenotypic, and genetic assessment and were stained with silver nitrate. In leukemic cells, the mean AgNORs number, mean AgNORs area, and m...
Article
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Dendritic cells (DCs) play a pivotal role in the homeostasis of the immune system. The tumor microenvironment impairs the proper function of DCs. The immunomodulatory properties of DCs in lung cancer are of interest. In the present study, we analysed DCs subsets and immune cells with the expression of immunomodulatory molecules: PD-1 and PD-L1 and...
Article
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Sarcoidosis (SA) is a systemic granulomatous disorder of unknown etiology with lung and mediastinal lymph nodes (LNs) as the main location. T lymphocytes play important role in the formation of granulomas in SA, but still little is known about the role of maturation profile in the development of inflammatory changes. The aim of this study was to de...
Article
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Different subpopulations of monocytes and dendritic cells (DCs) may have a key impact on the modulation of the immune response in malignancy. In this review, we summarize the monocyte and DCs heterogeneity and their function in the context of modulating the immune response in cancer. Subgroups of monocytes may play opposing roles in cancer, dependi...
Article
Minimal residual disease (MRD) status is now considered as one of the most relevant prognostic factors in multiple myeloma (MM) while MRD negativity became an important endpoint in clinical trials. Here, we report the results of the first study evaluating the reproducibility of high-sensitivity flow cytometry MM MRD assessment in four laboratories...
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Studying the dynamics changes of neutrophils during innate immune response in coro-navirus 2019 (COVID-19) can help understand the pathogenesis of this disease. The aim of the study was to assess the usefulness of new neutrophil activation parameters: Immature Granulocyte (IG), Neutrophil Reactivity Intensity (NEUT-RI), Neutrophil Granularity Inten...
Article
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Most patients with primary immune deficiency suffer from recurrent infections; however, paradoxical autoimmune phenomena can also manifest. The aim of this study was to identify immunological markers of autoimmune phenomena associated with common variable immunodeficiency (CVID). The study included 33 adults with CVID divided into two groups: (1) t...
Article
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Lung cancer remains one of the most aggressive solid tumors with an overall poor prognosis. Molecular studies carried out on lung tumors during treatment have shown the phenomenon of clonal evolution, thereby promoting the occurrence of a temporal heterogeneity of the tumor. Therefore, the biology of lung cancer is interesting. Cancer stem cells (C...
Article
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The role of the adaptive microenvironment components in severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) infection is widely researched, but remains unclear. Studying the common dynamics of adaptive immune response changes can help understand the pathogenesis of coronavirus disease 2019 (COVID-19), especially in critical patients. The a...
Article
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Background: The efficacy of immune checkpoint inhibitors (ICIs) remains unexpected and in some patients the resistance to anti-programmed death-1 (anti-PD-1) and anti-programmed death ligand 1 (anti-PD-L1) agents is observed. One of possible explanation may be PD-L2 activity. PD-1 ligands: PD-L1 and PD-L2 are present on cancer cells but also, not...
Article
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Identification of patients with activation of the immune system which indicates the presence of infection is essential, especially in the times of the global coronavirus 2019 (COVID-19) pandemic. The aim of the present study was to evaluate the reactive lymphocytes (RE-LYMP) parameter in COVID-19 and to correlate it with activation lymphocytes mark...
Article
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Cell response to novel coronavirus disease 19 (COVID-19) is currently a widely researched topic. The assessment of leukocytes population and the maturation of both B and T lymphocytes may be important in characterizing the immunological profile of COVID-19 patients. The aim of the present study was to evaluate maturation of B and T cells in COVID-1...
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Background The Mindray BC‐6200 is a new automatic hematology analyzer that quantifies the parameters of blood morphology and leukocyte differential in five populations (5‐Diff). The aim of the study was to evaluate the BC‐6200 and compare it with the Siemens ADVIA 2120i analyzer. Materials and Methods The comparison between BC‐6200 and ADVIA 2120i...
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Background Sarcoidosis is a multisystemic granulomatous disease with still unknown etiology. Our previous studies showed a significantly higher percentage of CD34 + cells in the peripheral blood in patients with sarcoidosis (SA) compared to the control group. The objective of the present study was to characterized of the CD34 + cell population in p...
Article
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(1) The cells from the monocyte line play an important role as regulators of cancer development and progression. Monocytes present pro- and anti-tumor immunity and differentiation into macrophages. Macrophages are predominant in the lung cancer environment and could be evaluated by bronchoalveolar lavage fluid (BALF). (2) The aim of the study was a...
Article
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Over the past decade, immune checkpoint inhibitors have revolutionized the treatment of non-small cell lung cancer (NSCLC). Unfortunately, not all patients benefit from PD-(L)1 blockade, yet, the PD-L1 tumor cell expression is the only approved biomarker, and other biomarkers have been investigated. In the present study, we analyzed the presence of...
Article
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The overexpression of programmed death-1 (PD-1) and cytotoxic T cell antigen 4 (CTLA-4) receptors on T cells are among the major mechanisms of tumor immunoevasion. However, the expression pattern of these receptors on T cell subpopulations of a different activation status and at different sites is poorly characterized. Thus, we analyzed the express...
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Introduction: M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF). Aim of the study: To find differences between BALF from lung affected by cancer (clBALF) and hlBALF from th...
Article
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Objective: Local cytokine milieu (especially Th2 inflammatory type) secreted into the asthmatic airways affect the alternative activated macrophages polarization (M2). TSLP and IL-33 are important alarmins of allergic response associated with Th2 inflammation. The aim of the study was to investigate the expression of the receptors for epithelial de...
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Purpose Obstructive sleep apnea syndrome (OSAS) is associated with alterations in immune system which may lead to serious complications. The aim of this study was to explore lymphocyte populations in OSAS with special attention to the Fas-positive cells. Methods Fifty-one patients with confirmed OSA and 20 healthy subjects were investigated. The O...
Article
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A balance between tumor invasion and immune defence system is widely investigated. Objective . The aim of this study was to evaluate lymphocyte phenotype in lymph nodes (LNs) of patients with lung cancer in relation to the presence of metastases. Methods . We investigated 364 LNs resected by transcervical extended mediastinal lymphadenectomy (TEMLA...
Article
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Regulatory T cells (Tregs) play an important role in the suppression of the immune response in lung cancer. Cytotoxic T-lymphocyte antigen 4 (CTLA-4) expressed on T lymphocytes is capable of downregulating cytotoxic T cells and is constitutively expressed on Tregs. Little is known about the population of Tregs with two forms of CTLA-4: surface (s)...
Article
There are many mechanisms that allow to escape tumour cells from immune surveillance. It is known that T regulatory cells (Tregs) play a crucial role in the inhibition of immune response. Th17 cells are defined by production of IL17A and have pleiotropic function. The Tregs and Th17 interaction in local environment of lung cancer remain unclear. Th...
Article
There are many mechanisms that allow to escape tumour cells from the immune surveillance. It is known that T regulatory cells (Tregs) play a crucial role in the inhibition of immune response. Th17 cells are defined by production of IL17A and have pleiotropic function. The role of Th17 cells and Tregs and Th17 interaction in local environment of lun...
Article
The aim of the study was to compare the presence of regulatory T cells (Tregs) in the local lung cancer environment versus systemic immune response based on the examination of bronchoalveolar lavage fluid (BALf) and peripheral blood (PB) from the same patient. 35 patients with lung cancer were investigated. Flow cytometry method with panel of antib...
Article
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The role of bronchoalveolar lavage fluid (BALf) examination in differential diagnosis of interstitial lung diseases (ILD) was established. Currently, functional polarization into M1 (pro-inflammatory) and M2 (anti-inflammatory) subpopulations is emphasized. The aim of our study was to compare the proportion of M1 and M2 in BALf of patients with dif...
Poster
Regulatory T cells (Tregs) play an important role in anti-cancer response. Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) is a molecule expressed on T lymphocytes which is capable to down-regulate cytotoxic T cells and is constitutively expressed on Tregs. Little is known about the lung population of Tregs bearing CTLA-4 in lung cancer. There are two fo...
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Forkhead transcription factor (FoxP3) associated with regulatory T cells is involved in the anti-cancer immune response regulation. The aim of study was to evaluate lymphocyte phenotype and the expression of FoxP3 in lymph nodes (LN) of patients with lung adenocarcinoma in relation to presence of metastases. 82 lymph nodes station 7 resected by Tra...
Article
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NKT cells form an important subset of lymphocytes with immunoregulatory properties which participate in the pathophysiology of cardiovascular and respiratory disorders. The aim of our study was to assess how the oxygenation-reoxygenation cycles and coexisting disorders with OSAS (the obstructive sleep apnoea syndrome) affect the distribution of NKT...
Article
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Breathing disorders during sleep increase the risk of many serious complications and pathophysiological abnormalities. The obstructive sleep apnoea syndrome (OSAS) and related comorbidities are accompanied by systemic inflammation. In our conducting study on immune response in OSAS we observed significant changes related to comorbidities. Thus we c...
Article
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Perforin plays an essential role in cytotoxicity of natural killers (NK) and CD8+ lymphocytes. Cytotoxicity of T and NK cells is one of the mechanisms of destruction of cells in Hashimoto's disease (HD). The aim of this study was analysis of the expression of perforin in CD8+, CD4+, and NK cells and cytotoxic abilities of these cells in children wi...
Article
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Background: Obstructive sleep apnoea syndrome (OSAS) brings risk of serious complications. The study objective was to assess elements of the cellular immune response in the course of OSAS. Methods: Peripheral blood (PB) lymphocytes: T, B, NK, NKT-like, Th, Tc, and HLA DR+ T cells were evaluated by flow cytometry of 48 OSA patients; the concentra...
Article
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INTRODUCTION Sarcoidosis (SA) is a granulomatous disease which most often affects the lung. The role of alveolar macrophages (AMs) in SA granuloma formation was established. Recently, the two macrophage populations are being described: M1 and M2. In our studies we focused on the influence of tobacco smoking on SA. As the number of AMs in the lung o...
Article
Full-text available
Introduction: Sarcoidosis is a granulomatous disease, which most often affects the lungs. The role of alveolar macrophages (AMs) in granuloma formation in sarcoidosis has been established. Recently, 2 macrophage populations have been described: M1 and M2. In our styudy, we focused on the effect of tobacco smoking on sarcoidosis. The number of AMs...
Article
Full-text available
Lung cancer is the main cause of cancer death worldwide. An advanced stage of the disease at the time of diagnosis, observed in the majority of cases, does not allow for introduction of radical treatment or makes the treatment ineffective. Lung cancer as a solid tumour with a very low antigenicity escapes immune surveillance, and cytotoxic cells at...
Article
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Perforin is a glycoprotein responsible for pore formation in cell membranes of target cells. Perforin is able to polymerize and form a channel in target cell membrane. Many research groups focus on the role of perforin in various diseases, immune response to bacterial and viral infections, immune surveillance and immunopathology. In addition, perfo...
Article
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Lung cancer is a leading cause of cancer deaths. As a solid tumor with low antigenicity and heterogenic phenotype lung cancer evades host immune defense. The cytotoxic anticancer effect is suppressed by a complex mechanism in tumor microenvironment. The population of regulatory T cells (Tregs) plays a crucial role in this inhibition of immune respo...