
Ivo Safarik- Czech Academy of Sciences, Biology Centre
Ivo Safarik
- Czech Academy of Sciences, Biology Centre
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Publications (296)
Textile solid-phase extraction (TSPE) employing an acrylic nonwoven textile impregnated with the sulfated, negatively charged marine polysaccharide ulvan was employed for the extraction of four positively charged dyes, namely safranin O, methylene blue, malachite green and crystal violet, from aqueous solutions. The extracted dyes were analyzed by...
Here we examine theoretically the possibility of determining the
magnitude of the magnetic field in a hydrogen plasma based on the
resonant Faraday rotation of the polarization plane of light by residual
natural atoms in the plasma (or by specially introduced impurity
atoms).
Copper chitosan attached to a magnetic synthetic nonwoven textile was manufactured using a simple, rapid, and green procedure employing chitosan dissolved in diluted acetic acid and treatment with copper sulfate solution. The prepared copper-chitosan-modified textile exhibited peroxidase-mimetic activity which was subsequently used for the degradat...
Dielectric properties of native nonwoven textile as well as textile with bound biochar and multi-walled carbon nanotubes in the frequency range of 10 to 5·105 Hz and at the temperatures of 30 to 60 °C have been investigated. The capacity of native nonwoven textile has been shown to decrease with the temperature according to the Arrhenius law. The a...
Color catcher sheets, developed to prevent color runs during the washing process, have found interesting applications in (bio)analytical chemistry. Both native and modified sheets can be used as low-cost, planar optical sensors for the analysis of various dyes and as a carrier for immobilization of specific ligands. Changes of sensor color can be o...
A study of the role of diamond nanoparticles on 5CB liquid crystal composites with Fe3O4 nanoparticles is presented. Composite ferronematic systems based on the nematic liquid crystal 5CB doped with Fe3O4 magnetic nanoparticles and additionally bound to diamond nanoparticles (DNPs), of a volume concentration of 3.2 mg/mL, 1.6 mg/mL and 0.32 mg/mL,...
Montmorillonite (Mnt), belonging to the smectite group and representing a 2:1-type clay mineral with good cation-exchange and swelling capacities, is a common clay component of soils. It was observed that some Mnt preparations exhibit peroxidase-like (P-L) activity using N,N-diethyl-p-phenylenediamine sulfate salt as a substrate. Both native (non-s...
Bioassays are the main tool to decipher bioactivities from natural resources thus their selection and quality are critical for optimal bioprospecting. They are used both in the early stages of compounds isolation/purification/identification, and in later stages to evaluate their safety and efficacy. In this review, we provide a comprehensive overvi...
Fifteen macroalgae collected at the Gulf of Saros, Turkey, were tested for the dye adsorption. Best results were obtained with Ulva rigida biomass. Magnetic modification of U. rigida biomass was performed with iron oxide microparticles synthesized by microwave irradiation of ferrous sulfate solution at high pH. Both native and magnetically modified...
Розглядається можливiсть створення на основi бiнарного водного розчину полiмерних та олiгомерних молекул гiдрогелiв нового типу – ламелярно-ланцюгових – iз каркасом пiдвищеної жорсткостi. Запропоновано гiпотетичну модель такого каркасу, структурними елементами якого є олiгомернi ламели та полiмернi ланцюги. Цi елементи з’єднуються завдяки тому, що...
Liquid marbles, droplets entrapped in a solid particle shell at the air-liquid interface, can respond to stimuli such as alternating magnetic fields. In this study, we examined liquid marbles composed of the ferrofluid core covered by diverse particles, including thermo-responsive polymer microspheres. The temperature elevation induced by an altern...
The possibility of creating hydrogels of a new lamellar-chain type in the framework of increased rigidity on the basis of ternary aqueous solutions of polymeric and oligomeric molecules has been considered. A hypothetical model of such a framework whose structural elements are oligomeric lamellas and polymer chains is proposed. These elements are c...
Per-and polyfluorinated alkyl substances (PFAS) have long been known for their detrimental effects on the ecosystems and living organisms; however the long-term impact on the marine environment is still insufficiently recognized. Based on PFAS persistence and bioaccumulation in the complex marine food network, adverse effects will be exacerbated by...
Magneto-responsive textiles have emerged lately as an important carrier in various fields, including biomedical engineering. To date, most research has been performed on single magnetic fibers and focused mainly on the physical characterization of magnetic textiles. Herein, from simple woven and non-woven textiles we engineered materials with magne...
Aquaculture has been one of the fastest‐growing food production systems sectors for over three decades. With its growth, the demand for alternative, cheaper and high‐quality feed ingredients is also increasing. Innovation investments on providing new functional feed alternatives have yielded several viable alternative raw materials. Considering all...
Solid phase extraction, based on the use of color catcher sheets, was successfully employed for the preconcentration of food dyes from alcohol containing beverages. The photos of color catcher sheets with the adsorbed dyes were taken with a mobile phone. The smartphone-based image analysis of the photos was performed using Color Picker application....
In environmental and agronomic settings, even minor imbalances can trigger a range of unpredicted responses. Despite the widespread use of metal-based nanoparticles (NPs) and new bio-nanofertilizers, their impact on crop production is absent in the literature. Therefore, our research is focused on the agronomic effect of spray application of gold n...
The COVID-19 pandemic has hugely affected the textile and apparel industry. Besides the negative impact due to supply chain disruptions, drop in demand, liquidity problems, and overstocking, this pandemic was found to be a window of opportunity since it accelerated the ongoing digitalization trends and the use of functional materials in the textile...
Neurodegenerative disorders, including Alzheimer’s disease (AD), Parkinson’s disease (PD), or systemic amyloidosis, are characterized by the specific protein transformation from the native state to stable insoluble deposits, e.g., amyloid plaques. The design of potential therapeutic agents and drugs focuses on the destabilization of the bonds in th...
The hyperthermia efficiency itself and the heating effect are determined not only by the material properties but also by the technical-application factors. For this reason, we subjected our experimental materials to experiments in an alternating magnetic field (AMF) as well as in a rotating magnetic field (RMF). According to the known numerical mod...
A number of materials are studied in the field of magnetic hyperthermia. In general, the most promising ones appear to be iron oxide particle nanosystems. This is also indicated in some clinical trial studies where iron-based oxides were used. On the other hand, the type of material itself provides a number of variations on how to tune hyperthermia...
The forensic scenario, on which the round robin study was based, simulated a suspected intentional manipulation of a real estate rental agreement consisting of a total of three pages. The aims of this study were to (i) establish the amount and reliability of information extractable from a single type of evidence and to (ii) provide suggestions on t...
Nanoparticles can be used in numerous therapeutic procedures, including controlled drug delivery and release, thermal therapy, and photo- and sonodynamic therapy. However, such approaches remain limited due to difficulties associated with accurately controlling their therapeutic efficiency and localized consequences. Thus, we propose the use of ult...
Copper silicate (CS) bound to a nonwoven textile was prepared using a very simple procedure employing water glass as a precursor and subsequent copper sulfate treatment. Peroxidase‐like activity of the textile‐based CS derivative was detected for the first time. This new nanozyme was employed for the decolorization of three model organic dyes: Bism...
Magnetically responsive minerals were isolated from soil samples using a strong permanent magnet. The isolated materials exhibited peroxidase-like activity using N,N-diethyl-p-phenylenediamine sulfate salt as a substrate. Autoclaving and hot air treatment at 200 °C did not substantially decreased the enzyme-like activity; on the contrary thermal tr...
The current demand for alternative water sources requires the incorporation of low-cost composites in remediation technologies; these represent a sustainable alternative to more expensive, commercially used adsorbents. The main objective of this comprehensive field-scale study was to incorporate the layered double hydroxides (LDHs) into the hybrid...
Aniline (benzenamine) is a very important substance used in industry even though it is classified as very toxic. The need for a practical solution to remove it from the environment is still a current topic. Aniline adsorption from water solution was evaluated using low-cost, magnetically responsive adsorbents of different type (plant derived materi...
Tubular halloysite (HNT) is a naturally occurring aluminosilicate clay with a unique combination of natural availability, good biocompatibility, high mechanical strength, and functionality. This study explored the effects of magnetically responsive halloysite (MHNT) on the structure, morphology, chemical composition, and magnetic and mechanical pro...
Biomass is defined as organic matter from living organisms represented in all kingdoms. It is recognized to be an excellent source of proteins, polysaccharides and lipids and, as such, embodies a tailored feedstock for new products and processes to apply in green industries. The industrial processes focused on the valorization of terrestrial biomas...
Various types of biological materials including agriculture and food waste and byproducts can be used as biosorbents for dye removal from contaminated waters. Magnetic modification of biosorbents enables to prepare smart materials exhibiting response to external magnetic field. Due to the presence of magnetic particles on or within the modified bio...
Fluorescent and magnetic nano- and microparticles have already been used for wide range of
(bio)applications. Multimodal imaging as well as simultaneous control and monitoring of intracellular
movements profit from the combination of these functionalities. Accordingly, several methods have been
developed for the synthesis of magnetic fluorescent pa...
A polymer brush system of a neutral polymer poly (ethylene glycol) with a molecular weight of Mw = 20 kDa on silicon substrates in an aqueous medium was studied by the specular neutron reflectometry. Structural changes in the density profile of a polymer brush caused by the interaction of polymer chains with micelles of the anionic surfactant dodec...
The determination of contaminants of emerging concern (CECs) in environmental samples has become a challenging and critical issue. The present work focuses on miniaturized analytical strategies reported in the literature for the determination of CECs. The first part of the review provides brief overview of CECs whose monitoring in environmental sam...
Abiotic and biotic remediation of chlorinated ethenes (CEs) in groundwater from a real contaminated site was studied using biochar-based composites containing nanoscale zero-valent iron (nZVI/BC) and natural resident microbes/specific CE degraders supported by a whey addition. The material represented by the biochar matrix decorated by isolated iro...
Coastal countries have traditionally relied on the existing marine resources (e.g., fishing, food, transport, recreation, and tourism) as well as tried to support new economic endeavors (ocean energy, desalination for water supply, and seabed mining). Modern societies and lifestyle resulted in an increased demand for dietary diversity, better healt...
Coastal countries have traditionally relied on the existing marine resources (e.g., fishing, food, transport, recreation, and tourism) as well as tried to support new economic endeavors (ocean energy, desalination for water supply, and seabed mining). Modern societies and lifestyle resulted in an increased demand for dietary diversity, better healt...
Coastal countries have traditionally relied on the existing marine resources (e.g., fishing, food, transport, recreation, and tourism) as well as tried to support new economic endeavors (ocean energy, desalination for water supply, and seabed mining). Modern societies and lifestyle resulted in an increased demand for dietary diversity, better healt...
Magnetic textile solid phase extraction, based on the use of magnetically separable color-catching sheets, was successfully employed for the preconcentration of water-soluble dyes methylene blue and crystal violet from water solutions. The photos of color-catching sheet squares with the adsorbed dye were taken with a mobile phone. The computer-base...
The presence of heavy metals in environmental waters having an important place in the industrial waste is a major threat
to viability. Heavy metals are transported to humans through the ecological cycle, damaging many tissues and organs. In recent years,
agricultural and food waste can be used to remove heavy metals. At the present study, magnetica...
Magnetically responsive materials have been successfully used for preconcentration of enormous amount of target analytes from diluted solutions or suspensions. In this short review chapter we will present the basic information about the newly developed preconcentration procedure called magnetic textile solid-phase extraction. This simple, low-cost...
Novel method for rapid magnetic modification of diamagnetic powder and high aspect ratio materials has been developed. This procedure is based on thorough mixing of diamagnetic materials with suspensions of magnetite nano-and microparticles in highly volatile organic solvents (namely n-pentane or petroleum ether) followed by a drying step. Due to t...
The determination of contaminants of emerging concern (CECs) in environmental samples has become a challenging and critical issue. The present work focuses on miniaturized analytical strategies reported in the literature for the determination of CECs. The first part of the review provides brief overview of CECs whose monitoring in environmental sam...
Magnetically responsive acrylic nonwoven textile impregnated with negatively charged κ-carrageenan was used as an adsorbent for the extraction of cationic dyes using magnetic textile solid phase extraction (MTSPE). Polysaccharide modified textile squares were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy;...
Applications of nanomaterials to biological systems have received increased interest in the past decades. In particular, bacterial cellulose nanofibers can be utilized in a broad range of applications. Biomaterials have been produced on large-scale with high reproducibility by an inspiring method such as molecular imprinting. Herein, for the first...
Magnetic fluids (ferrofluids) have found many important applications in various areas of bio-sciences, biotechnology, medicine, and environmental technology. In this review, we have summarized the relevant information dealing with a magnetic modification of diamagnetic materials using different types of ferrofluids. Special attention is focused on...
Microwave synthesized iron oxide nanoparticles and microparticles were used to prepare a magnetically responsive biosorbent from Rhytidiadelphus squarrosus moss for the rapid and efficient removal of Co²⁺ ions and thioflavin T (TT). The biocomposite was extensively characterized using Fourier transformed infrared (FTIR), XRD, SEM, and EDX technique...
Several methodologies for the preparation of nanofibrous materials exist. Electrospinning is currently the most popular technique due to its versatility and simplicity. Nanofibrous materials prepared in such a way are widely studied in medicine and material engineering. Polyvinyl butyral (PVB) nanofibers were generated by a rod-shaped spinning-elec...
Magnetic nano- and microparticles have been successfully used in many areas of algae biotechnology, especially for harvesting of algal biomass, separation of algal biologically active compounds, immobilization of algal cells, removal of important xenobiotics using magnetically modified algae, or for the preparation of magnetic catalysts; alternativ...
Marine organisms produce a vast diversity of metabolites with biological activities useful for humans, e.g., cytotoxic, antioxidant, anti-microbial, insecticidal, herbicidal, anticancer, pro-osteogenic and pro-regenerative, analgesic, anti-inflammatory, anti-coagulant, cholesterol-lowering, nutritional, photoprotective, horticultural or other benef...
Microalgae, macroalgae and seagrass biomass can be considered as functional materials enabling various biotechnology and environmental technology applications. Using an appropriate magnetic modification, smart materials exhibiting a rapid response to an external magnetic field can be obtained. Such composite materials can be easily and selectively...
Various materials have been extensively investigated to mimic the structures and functions of natural enzymes. We describe discovery of a new catalytic property in the group of biochar-based carbonaceous materials, which are usually produced during biowaste thermal processing under specific conditions. The tested biochars exhibited peroxidase-like...
Bisphenol A was efficiently adsorbed on biochar magnetically modified with microwave-synthesized magnetic iron oxide particles. The adsorption followed the Langmuir isotherm model, and the maximum adsorption capacity was 77.4 mg per gram of magnetic biochar at 282.15 K. Kinetics of sorption process followed the pseudo-second-order kinetic model, an...
Textile modified with methyltrioctylammonium thiosalicylate ionic liquid was successfully used for Magnetic textile solid phase extraction of copper ions. After extraction process the modified textile changed color to gray and black. The textile photos were employed for image analysis using HSB and RGB color spaces. It was shown that values of satu...
Novel modern easily feasible methods for direct evaluation of a new, simple preconcentration analytical procedure have been developed. Two types of smartphone image analysis applications (ON Color Measure and Color Lab) were evaluated to obtain RGB and HSV color spaces data for the quantification of Magnetic textile solid phase extraction of colore...
Poly(glycidyl methacrylate) (PGMA) is prone to modifications with different functional groups, magnetic fluids or direct coupling with biological molecules. The purpose of this research was to synthesize new magnetically responsive particles with peroxidase-like activity. Poly(glycidyl methacrylate-co-ethylene dimethacrylate) [P(GMA-EDMA)] particle...
Magnetic derivative of green alga Cymopolia barbata was prepared. • Microwave-synthesized magnetic iron oxide particles was used for modification. • Magnetic Cymopolia barbata biomass was used for safranin O removal. A R T I C L E I N F O Keywords: Cymopolia barbata Adsorption Magnetic adsorbent Microwave synthesis Safranin O A B S T R A C T The pu...
The magnetic modification of four different electrospun nanofibrous textiles, based on polyamide, polyvinyl alcohol, polycaprolactone and polyurethane, is reported. The simple spray modification procedure employing two types of magnetic fluids was used. Magnetic modification led to the deposition of magnetic iron oxide nanoparticles on the surface...
Magnetic textile solid phase extraction, based on the use of magnetically modified non-woven textile impregnated with chitosan, was successfully employed for the preconcentration of acid food dyes from water solutions. The photos of textile squares with the adsorbed dye were taken with a mobile phone. The image analysis of the photos was performed...
Comprehensive processing of Japonochytrium sp., one of marine fungi considered as a perspective source of functional lipids, was tested as an example of circular economy. Residual biomass after extraction of lipids (omega-3 polyunsaturated fatty acids and triacylglycerols) was magnetically modified using methanol suspension of microwave-synthesized...
Magnetically responsive materials have found many important applications in analytical chemistry. In this short review the basic information about Magnetic solid phase extraction and Magnetic textile solid phase extraction is given. These analytical techniques enable to preconcentrate target biologically active compounds or pollutants from water sa...
Magnetically responsive materials have found many important applications in analytical chemistry. In this short review the basic information about Magnetic solid phase extraction and Magnetic textile solid phase extraction is given. These analytical techniques enable to preconcentrate target biologically active compounds or pollutants from water sa...
A R T I C L E I N F O Keywords: Chitosan Magnetic textile solid phase extraction Nonwoven textile Acid food dyes A B S T R A C T Chitosan impregnated, polyacrylonitrile nonwoven textile formed from micrometer sized fibres (diameter 15 μm), was used for a newly developed analytical procedure named Magnetic Textile Solid Phase Extraction (MTSPE). Thi...
Magnetic derivative of green alga Cymopolia barbata was prepared. • Microwave-synthesized magnetic iron oxide particles was used for modification. • Magnetic Cymopolia barbata biomass was used for safranin O removal. A R T I C L E I N F O Keywords: Cymopolia barbata Adsorption Magnetic adsorbent Microwave synthesis Safranin O A B S T R A C T The pu...
Microalgae accumulate a variety of compounds that form the basis for potential marketable products as whole cell biomass or as fractions after an extraction process. A cascading biorefinery approach aims at recovering and separating multiple algae compounds to valorise the complete biomass and to improve the economics of the value chain. This chapt...
The temperature-dependent biosynthesis of gold nanoparticles (AuNP) using diatom cells of Diadesmis gallica was successfully performed. The resulting biosynthesis product was a bionanocomposite containing AuNP (app. 20 nm) subsequently anchored on the silica surface of diatomaceous frustules. As-prepared nanogold-biosilica composite was tested as c...
The microbial synthesis of biominerals offers a potentially sustainable green solution for the production of a wide range of industrially relevant functional nanomaterials. Metal-reducing bacteria are of particular relevance, as they can enzymatically reduce a wide spectrum of high oxidation state metals and metalloids, forming cell-templated nanom...
Non-magnetic TiO2 powder and magnetically modified TiO2 powder were employed for sorption experiments; the magnetically modified TiO2 particles allow magnetic separation of the sorbents with already adsorbed ions of pollutants. FeSO4.7H2O was used for synthesis of magnetically responsive TiO2. A detailed study of the adsorption/desorption process w...
Two clay minerals of the similar 2: 1 layer structure and chemical composition, vermiculite and montmorillonite, were studied using a wide spectrum of experimental methods in their original states and the magnetically modified states after mixing with microwave-synthesized iron oxide particles. This magnetic modification led to different microstruc...
A new preconcentration procedure named ''Magnetic textile solid phase extraction'' was developed. A piece of nonwoven textile (fabric) was used for the immobilization of copper phthalocyanine dye and employed for the adsorption of crystal violet and safranin O from large volumes of sample. The bound dye was eluted into small volume of methanol. The...
The fabrication of magnetically modified elec-trospun nanocomposite fibers based on a naturally-derived biocompatible and biodegradable polysaccharide chitosan (CS) and the hydrophilic and biocompatible poly(vinylpyrrolidone) (PVP) is reported herein. The anchoring of magnetic nanoparticles (MNPs) onto the surfaces of the electrospun PVP/CS fibers...
Manganese dioxide microparticles were adsorbed on non-woven fabric fibers. This low-cost composite material was used as a recoverable catalyst for hydrogen peroxide decomposition. Magnetically responsive composite catalyst was prepared by the insertion of a steel staple in the fabric material.
A recently developed preconcentration procedure “magnetic textile solid-phase
extraction”, based on the use of a magnetically modified textile impregnated with chitosan,
was successfully employed for the preconcentration of blue fountain ink dye
Acid blue 93 from extremely diluted solutions. This procedure enabled to detect ink
dye released from in...
Silver nanoparticles released from a variety of consumer products represent a hazard for the environment. Their removal using appropriate low-cost adsorbents is a progressive way of economical treatment of the polluted environment, especially water sources. Magnetically responsive materials form a progressive group of ad-sorbents, that is why magne...
The annual food and agricultural waste production reaches enormous numbers. Therefore, an increasing need to valorize produced wastes arises. Waste materials originating from the food and agricultural industry can be considered as functional materials with interesting properties and broad application potential. Moreover, using an appropriate magnet...
in the biomedical field and they have been extensively studied in drug delivery [1-11] and tissue engineering [12-16] applications , due to their highly hydrated 3D architecture, biodegradability/biocompat-ibility, tunable mechanical properties, and stimuli responsiveness. During the last years, researchers have been focusing on new synthetic strat...
Silver nanoparticles released from a variety of consumer products represent a hazard for the environment. Their removal using appropriate low-cost adsorbents is a progressive way of economical treatment of the polluted environment, especially water sources. Magnetically responsive materials form a progressive group of ad-sorbents, that is why magne...
The interaction of amyloid β-peptide (Aβ) with the iron-storage protein ferritin was
studied in vitro. We have shown that Aβ during fibril formation process is able to reduce
Fe(III) from the ferritin core (ferrihydrite) to Fe(II). The Aβ-mediated Fe(III) reduction yielded a two-times-higher concentration of free Fe(II) than the spontaneous formati...
Background:
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction cycle to another, as high centri...
The work explores preparation of new biocompatible magnetic halloysite nanotubes (MHNTs) reinforced polycaprolactone/gelatine nanofibres. MHNTs were prepared by mixing halloysite nanotubes (HNTs) with microwave-synthesized magnetic iron oxide particles suspension. Electrospun nanofibres comprising 8 wt % poly ɛ-caprolactone (PCL) and 8 wt % gelatin...
The work explores preparation of new biocompatible magnetic halloysite nanotubes (MHNTs) reinforced polycaprolactone/gelatine nanofibres. MHNTs were prepared by mixing halloysite nanotubes (HNTs) with microwave-synthesized magnetic iron oxide particles suspension. Electrospun nanofibres comprising 8 wt % poly ɛ-caprolactone (PCL) and 8 wt % gelatin...
Non-magnetic TiO 2 powder and magnetically modified TiO 2 powder were employed for sorption experiments; the magnetically modified TiO 2 particles allow magnetic separation of the sorbents with already adsorbed ions of pollutants. FeSO 4 .7H 2 O was used for synthesis of magnetically responsive TiO 2. A detailed study of the adsorption/desorption p...
Smart materials of biological origin are attracting a lot of attention nowadays, especially as catalysts, carriers or adsorbents. Among them, magnetically modified biomaterials are especially important due to their response to external magnetic field. This report demonstrates that naturally occurring micrometer sized, high aspect ratio material (na...
Thanks to the content of various high-value products (antioxidants, vitamins, fatty acids, oils, polysaccharides, etc.) and simple cultivation in large scale, microalgae have become an increasingly popular and utilized group of photosynthetic organisms with wide range of possible use in diverse areas of biosciences, biotechnology, food technology,...
Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was magnetically modified using perchloric acid stabilized magnetic fluid. Magnetic bacterial cellulose (MBC) was used as a carrier for the immo-bilization of affinity ligands, enzymes and cells. MBC with immobilized reactive copper phthalocyanine dye was an efficient adsorbent f...
A simple, one-pot process for the preparation of magnetically responsive yeast-based biocatalysts was developed. Saccharomyces cerevisiae, Candida utilis and Kluyveromyces lactis cells were successfully incorporated into chitosan gel magnetically modified with poly(methacrylic acid)-stabilized magnetic fluid (PMAA-FF) during its formation. Magnetic...
Protein transformation from its soluble state into amyloid aggregates is associated with amyloid-related diseases. Amyloid deposits of insulin fibrils have been found in the sites of subcutaneous insulin application in patients with prolonged diabetes. Using atomic force microscopy and ThT fluorescence assay we have investigated the interference of...
The goal of this study was to assess the biosorption of Amido black 10B dye from aqueous solutions on magnetically modified sheaths of Leptothrix sp. in a batch system. The magnetic modification of the sheaths was performed using both microwave synthesized iron oxide nano-and microparticles and perchloric acid stabilized ferrofluid. The native and...
Prokaryotic and eukaryotic cells can interact with various types of magnetic nanoparticles and microparticles. Due to the presence of magnetic particles on the cell surface, in protoplasm or in intracellular organelles, magnetically modified cells can be rapidly, easily, and selectively separated from desired environments by means of magnetic separ...
Cytokinins (CKs), pivotal plant hormones, play crucial roles in plant growth and development. However, their isolation and quantification are usually challenging because of their extremely low levels in plant tissues (pmol/g fresh weight). We have developed a simple microscale magnetic immunoaffinity-based method for selective one-step isolation of...
A simple, one-pot process for the preparation of magnetically responsive yeast-based biocatalysts was developed. Saccharomyces cerevisiae, Candida utilis and Kluyveromyces lactis cells were successfully incorporated into chitosan gel magnetically modified with poly(methacrylic acid)-stabilized magnetic fluid (PMAA-FF) during its formation. Magnetic...