Ivânia Esteves- PhD Biological Sciences, Cranfield University, UK
- Researcher at University of Coimbra
Ivânia Esteves
- PhD Biological Sciences, Cranfield University, UK
- Researcher at University of Coimbra
Nematology researcher, University of Coimbra, Portugal
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Introduction
I am currently developing research activities in the NEMATO-lab, Centre for Functional Ecology (CFE), at the Department of Life Sciences,University of Coimbra (UC), Portugal. The majority of the research since my PhD has been centered in finding alternative strategies to manage plant-parasitic nematodes. At present, I am coordinating an FCT project on the tropical root-knot nematode, Meloidogyne luci and I am involved in projects focusing studies on root-lesion nematodes infecting potato crops.
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Publications (49)
Nesta revisão evidencia-se o elevado potencial de utilização de nemátodes entomopatogénicos (NEPs) como agentes de proteção biológica na redução das populações de insetos fitófagos que podem causar estragos e/ou insetos vetores de doenças, em produção sustentável de alimentos, como alternativa ou complemento aos produtos fitofarmacêuticos. Descober...
Nesta revisão evidencia‑se o elevado potencial de utilização de nemátodes entomopatogénicos (NEP) como agentes de proteção biológica na redução das populações de insetos fitófagos que causam estragos e/ou insetos vetores de doenças, em produção sustentável de alimentos, como alternativa ou complemento aos produtos fitofarmacêuticos. Descobertos em...
Os nemátodes-das-galhas radiculares (NGR) são uma ameaça às culturas hortícolas em todo o mundo. O fungo Purpureocillium lilacinum (Thom) Luangsa-ard, oubraken, Hywel-Jones & Samson (=Paecilomyces lilacinus) reduz a infeção por NGR, pelo que a sua aplicação é vista como uma promissora alternativa aos nematodicidas de síntese química.
Plant-parasitic nematodes are highly damaging pests responsible for heavy losses in a considerable number of plant crops. Common pest management strategies rely on the use of synthetic chemical nematicides, which have led to serious concerns regarding their impact on human health and the environment. The essential oils (EOs) obtained from aromatic...
RESUMO O solo é um sistema dinâmico e um nicho de biodi-versidade que oferece suporte, refúgio e substrato para diferentes organismos. Quando saudável, o solo sustenta uma elevada biodiversidade, produ-tividade agrícola/florestal e contribui para vários serviços dos ecossistemas. Em solos alterados pelo homem é fundamental monitorizar a sua qualida...
The pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease and is considered an A2 quarantine organism by the European Plant Protection Organisation. In Europe, this nematode has been reported in Pinus pinaster, P. radiata, and P. nigra. In May 2024, severe wilting symptoms were observed in P. sylvestris trees...
The grey maize weevil, Tanymecus dilaticollis, is a polyphagous species, which is among the most important pests of maize
in Southeastern Europe. The efficacy of commercial products with two species of entomopathogenic nematodes (EPNs),
Steinernema carpocapsae and Heterorhabditis bacteriophora, was investigated against adults of the grey maize weev...
Os nemátodes fitoparasitas (NFP) podem alimentar-se de diversas partes das plantas, compreendendo as raízes, podendo parasitar inúmeras culturas, incluindo a videira (Vitis vinifera L.), afetando a qua-lidade e o volume da colheita e a longevidade das cepas. Em Portugal, apesar de não existirem dados oficiais sobre o impacto dos NFP na videira, as...
The root-knot nematode (RKN) Meloidogyne luci presents a threat to the production of several important crops. This nematode species was added to the European Plant Protection Organization Alert list in 2017. The scarce availability of efficient nematicides to control RKN and the phasing out of nematicides from the market have intensified the search...
Plant-parasitic nematodes (PPN), “the unseen enemies” of plants are a threat to the potato (Solanum tuberosum) production affecting their quality and yield. The most destructive species belong to the genera Globodera (Heteroderidae), Meloidogyne (Meloidogynidae), Pratylenchus/Nacobbus (Pratylenchidae), Ditylenchus (Anguinidae), and Nanidorus/Paratr...
The scarce availability of efficient and eco-friendly nematicides to control root-knot nematodes (RKN), Meloidogyne spp., has encouraged research toward the development of bionematicides. Naphthoquinones, juglone (JUG) and 1,4-naphthoquinone (1,4-NTQ), are being explored as alternatives to synthetic nematicides to control RKN. This study expands th...
The root-knot nematode (RKN) Meloidogyne luci is included in the Alert List of the European Plant Protection Organization, because it has potential negative impacts on economically important crops. Identification of plant species/cultivars resistant to M. luci is important for its management. Susceptibility of 35 commercial plant species/cultivars,...
Potato cyst nematodes (PCN), Globodera spp., cause damage to potatoes in more than 60 countries and several management strategies, including the application of chemical nematicides, are commonly used for their control. However, due to stringent regulations in Europe several nematicides have been, or are being, removed from the market due to their p...
The root lesion nematode Pratylenchus penetrans is an important plant-parasitic nematode of potato. In this study, the susceptibility of commercial potato cultivars to P. penetrans was assessed. Nematode penetration was evaluated in cultivars Agria, Camel, Kennebec, Laura, Royata, and Stemster at 1, 3, 7, and 15 days after inoculation (DAI) with 75...
Sweet potato, Ipomoea batatas L., is a tuberous root vegetable rich in low glycemic sugars, vitamins and fibers (Galvão et al., 2021). Although it is widely cropped and consumed in tropical regions, in Europe consumer demand is growing exponentially (CBI, 2021). In Portugal, the production area of sweet potato increased from 588 ha in 2011 to 954 h...
Simple Summary
The root knot nematode Meloidogyne luci has been identified in various countries around the world parasitizing economically important crops. Due to its potential to cause serious damage to agriculture, the need for an accurate diagnosis at the species level has become mandatory. In the present study, a specific amplification product...
The root lesion nematode Pratylenchus penetrans parasitizes a wide range of economic important crops, including potato (Solanum tuberosum L.). Damage by P. penetrans impacts not only the potato yield but can also reduce the tuber quality. Detailed information on tuber infection by P. penetrans is scarce for most cultivars and nematode molecular det...
Pratylenchus penetrans, one of the most detrimental root-lesion nematode species, greatly reduces the production in numerous important agronomic crops (e.g., corn, potato), ornamental plants (e.g., lily, roses) and fruit trees (e.g., almond, cherry orchards). In the EU, P. penetrans has been reported as the most damaging species associated with pot...
The root lesion Pratylenchus penetrans is an economically important pest affecting a wide range of plants. The morphometry of five P. penetrans isolates, parasitizing potato roots in Portugal, was compared and variability within and between isolates was observed. Of the 15 characters assessed, vulva position (V%) in females and the stylet length in...
Root-lesion nematodes, Pratylenchus spp., and root-knot nematodes, Meloidogyne spp., can occur concomitantly in potato fields and the interactions between the two groups of plant parasitic nematodes (PPN) may represent a threat to the potato crop. The in vitro reproductive fitness of 3 Portuguese isolates of P. neglectus isolated from potato fields...
The nematophagous fungus Pochonia chlamydosporia (Clavicipitaceae) is a facultative parasite of major plant-parasitic nematodes pests such as cyst (Globodera spp., Heterodera spp.), root-knot (Meloidogyne spp.), false root-knot (Nacobbus spp.) and reniform (Rotylenchulus reniformis) nematodes. The potential of P. chlamydosporia as a biological cont...
Production of Pochonia spp. in laboratory conditions has facilitated studies on its biology, abundance, dispersion, rhizosphere colonization, host preference, and isolate virulence. Research in biological control of nematodes requires suitable, standardized methods. In this chapter we review the commonest, non-molecular, standard in vitro culture m...
Culturing on agar is a common method used in fungal isolation, cultivation, preservation, microscopic examination, biochemical, and physiological characterization. The present chapter reviews research carried out on Pochonia chlamydosporia culturing under in vitro conditions, which have made possible the production of mycelium, conidia and chlamydo...
In 2013, during a field survey conducted in Portugal on potato, Solanum tuberosum L., an unusual esterase (EST) phenotype was detected in a root-knot nematode (RKN) from potato roots collected in Coimbra. This Portuguese isolate was purified and maintained on tomato, S. lycopersicum L., and studied by morphological, biochemical and molecular charac...
Naphthoquinones exhibit important biological activities and are present in walnut husks residues in significant amounts. However, their potential as alternatives to synthetic nematicides has not been fully explored. This work aimed to assess the effects of pure naphthoquinones (juglone; 1,4-naphthoquinone; plumbagin) on the root-knot nematode Meloi...
The root-lesion nematode Pratylenchus thornei is a widely distributed and important parasite of cereals and legumes. As migratory endoparasites, P. thornei are difficult to manage because they enter and leave host roots and may remain quiescent inside roots or in soil when conditions are unfavourable for plant growth. The number of available nemati...
Soil is composed of different types of particles which are either natural or of anthropogenic origin. Anthropogenic particles are often related to the presence of heavy metals and thus provide information on soil quality. Magnetic parameters can detect the presence of such particles and may be used as a proxy for environmental pollution. This study...
Root-lesion nematodes (RLN), Pratylenchus spp., are economically important plant-parasitic nematodes (PPN) of several crops, including potato. In Portugal, potato cyst nematodes (PCN), Globodera spp., are a frequent problem and root-knot nematodes (RKN), Meloidogyne spp., have been found coexisting with PCN. Although the diversity and pathogenicity...
Studies on the use of Pochonia chlamydosporia isolates as biological control agents of root-knot nematodes (RKN, Meloidogyne spp.), showed that their efficacy relies on selected key factors such as fungal proliferation in soil, establishment in the rhizosphere, and ability to parasitize RKN eggs. A pot experiment was conducted to evaluate the perfo...
The nematophagous fungus Pochonia chlamydosporia var. chlamydosporia is one of the most studied biological control agents against plant (semi-) endo-parasitic nematodes of the genera Globodera, Heterodera, Meloidogyne, Nacobbus and, more recently, Rotylenchulus. In this paper we present highlights from more than three decades of worldwide research...
Pochonia chlamydosporia, a widespread fungal parasite of potato cyst nematodes (PCN), Globodera spp., and root-knot nematodes (RKN), Meloidogyne spp., has been studied as a biological control agent. Three Portuguese isolates (Pc1, Pc2, Pc3) obtained from PCN eggs and two non-native isolates (Vc10, Pc280) were characterised using ERIC-PCR and screen...
The biocontrol potential of Pochonia chlamydosporia, a fungus with parasitic activity against economically important plant-parasitic nematodes, can be influenced by abiotic factors such as water availability. The objective of this study was to evaluate the effects of different water stress regimes on in vitro growth, sporulation, germination and pa...
The nematophagous fungus Pochonia chlamydosporia (Clavicipitaceae) can parasitize eggs of nematodes including cyst (Globodera spp., Heterodera spp.), root-knot (Meloidogyne spp.), false-root knot (Nacobbus spp.) and reniform (Rotylenchulus reniformis) nematodes. Its potential as a biological control agent has been the subject of numerous studies ai...
The effects of a host plant on reproduction/abundance of fungal populations in relation to soil nutrients released by plants in the rhizosphere were studied. Abundance in the soil and potato rhizosphere of the fungi Paecilomyces lilacinus, Monographella cucumerina (CABI 380408) and Pochonia chlamydosporia var. chlamydosporia (Pc280, potato cyst nem...
For the first time, the specific activities of chitinases, esterases, lipases and a serine protease (VCP1) produced by different isolates of the nematophagous fungus Pochonia chlamydosporia were quantified and compared. The isolates were grown for different time periods in a minimal liquid medium or media supplemented with 1 % chitin, 0.2 % gelatin...
The Cuban strain IMI SD 187 of the nematophagous fungus Pochonia chlamydosporia var. catenulata is a potential biological control agent for the root knot nematodes. There are reports about the controlling action of these fungi on cyst nematodes. However, isolate specificity according to the original host is also known. The aim of this work was to s...
For the first time, the effects of varying osmotic and matric potential on fungal radial growth and accumulation of polyols were studied in three isolates of Pochoniachlamydosporia. Fungal radial growth was measured on potato dextrose agar modified osmotically using potassium chloride or glycerol. PEG 8000 was used to modify matric potential. When...
Un aspecto importante en el desarrollo de un microorganismo como agente potencial de control biológico es disponer de un método de conservación adecuado para la cepa seleccionada, sin afectación de sus características. Entre los métodos más usados se encuentra los subcultivos seriados. El objetivo de este trabajo fue evaluar el efecto de esta práct...
DETECTION OF PROTEASES PRODUCED BY Pochonia chlamydosporia IN SOLID
MEDIUM
ABSTRACT: The detection of protease production is an important aspect for selecting some biological
control agents, because of the role these enzymes play in the infectious process. The aim of this work
was to modify a method for detecting proteases in solid medium supplemen...
STABILITY FOR Pochonia chlamydosporia var. catenulata IMI SD STRAIN (KAMYSCHO EX BARRON & ONIONS) ZARE & W. GAMS. PART II. BIOCHEMICAL INDICATOR
ABSTRACT: A fundamental aspect to develop a Biological Control Agent (BCA) is the genetic stability for the selected isolate for mass rearing and its application. Virulence can be attenuated by serial
sub...
