Ivan Lozada Lawag

University of Western Australia | UWA · School of Allied Health

This paper presents the findings of a comprehensive review on common bee pollen processing methods which can impact extraction efficiency and lead to differences in measured total phenolic content (TPC) and radical scavenging activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) data. This hampers the c...

The aim of this review is to provide a comprehensive overview of the large variety of phenolic compounds that have to date been identified in a wide range of monofloral honeys found globally. The collated information is structured along several themes, including the botanical family and genus of the monofloral honeys for which phenolic constituents...

This study reports on the development and validation of a HPTLC-derived database to identify phenolic compounds in honey. Two database sets are developed to contain the profiles of 107 standard compounds. Rich data in the form of Rf values, colour hues (H°) at 254 nm and 366 nm, at 366 nm after derivatising with natural product PEG reagent, and at...

This study reports on the total phenolic content and antioxidant activity as well as the phenolic compounds that are present in Calothamnus spp. (Red Bell), Agonis flexuosa (Coastal Peppermint), Corymbia calophylla (Marri) and Eucalyptus marginata (Jarrah) honeys from Western Aus-tralia. The honey's total phenolic content (TPC) was determined using...

The Folin-Ciocalteu assay is a widely used method for measuring the total phenolic content (TPC) in honey, but it can be affected by the presence of reducing sugars in honey, which can lead to interference and an over-estimation of its TPC. To optimize the Folin-Ciocalteu assay for honey analysis, the effect of pH on the assay was investigated. A n...

This study critically investigates the aluminium chloride–based colorimetric determination of the total flavonoid content (TFC) of honey. Following a comprehensive review of the recent literature reporting the use of the assay in the determination of TFC in honey, 10 honeys of different botanical origins were investigated using the colorimetric met...

This study is the first to report on the presence of oestrogenic compounds in different clover flower nectar samples, in bee-deposited nectars collected from hive combs (unripe honey) and in mature honeys harvested from the same hives. The clover species investigated were two red clover (Trifolium pratense) cultivars, bred specifically for high iso...

The aim of this study was to examine a collection of 79 honeys derived from plants endemic to several Western Australian unique bioregions for bioactivity and physicochemical characteristics. For physicochemical analyses, total phenolic content, high performance thin layer chromatography (HPTLC) fingerprints, pH, Brix, colour and hydrogen peroxide...

Variation in the antibacterial potency of manuka honey has been reported in several published studies. However, many of these studies examine only a few honey samples, or test activity against only a few bacterial isolates. To address this deficit, a collection of 29 manuka/Leptospermum honeys was obtained, comprising commercial manuka honeys from...

Honeys produced by Apis mellifera from different flowering plants can vary widely in their characteristics. Some floral sources yield honeys with especially high antibacterial and/or antioxidant activity, which may be candidates for further evaluation as therapeutic agents. This study investigated 115 honeys harvested from three Western Australian...

Despite its cultural and nutritional importance for local Aboriginal people, the unusual insect honey produced by Western Australian honeypot ant (Camponotus inflatus) has to date been rarely investigated. This study reports on the honey’s physicochemical properties, its total phenolic, major sugars and 5-hydroxymethylfurfural contents, and its ant...

This study reports on the analysis of eleven Jarrah (Eucalyptus marginata) honeys, of which nearly half (n = 5) were re-classified as Blackbutt (E. patens) honey on the grounds of the predominant flower pollen identified by melissopalynology. Based on a comprehensive analysis of the honeys’ physico- and phytochemical characteristics and antioxidant...

The bark of Ceriops tagal has been used in the Philippines for folk treatment of diabetes mellitus (DM). Through an α-glucosidase inhibition assay guided isolation, constituents from the detannified methanolic bark extract were isolated and identified to be methyl 2,4-dihydroxy-3,6- dimethyl benzoate (1), isopimar-7-ene-15S,16-diol (2), betulinic a...

Background Rhizophora mucronata (Rhizophoraceae) is being used for the treatment of different disorders including diabetes in coasts of indian Ocean and the West-Pacific since long. However, there is scarcity of scientific data to validate local claims on the use of R mucronata as antidiabetic as well as lack of data about its acute toxic effect. A...

Solid waste generation had been increasing and had been a serious problem in the Philippines due to population growth and urbanization. Schools had been a major contributor of the said residual wastes that are being collected. There are a lot of environmental advocacies that were attributed to prper waste segregation and solid waste management but...

Antidesma bunius Spreng. (Phyllantaceae), Averrhoa bilimbi L. (Oxalidaceae), Biophytum sensitivum (L.) DC. (Oxalidaceae), Ceriops tagal (Perr.) C.B. Rob. (Rhizophoraceae), Kyllinga monocephala Rottb. (Cyperaceae), and Rhizophora mucronata Lam. (Rhizophoraceae) are used as remedies to control diabetes. In the present study, these plants were screene...

The study that was pursued aimed to screen ethnopharmacologically used antidiabetic plants found in the Philippines for their potential yeast alpha glucosidase enzyme inhibitory activity. Initially during the preliminary phase of this research, 148 plants were shortlisted as plants found in the Philippines as antidiabetic remedies. The plants were...

Diabetes is one of the ten (10) most prevalent chronic diseases not only in the Philippines but also in the whole world. Thus, a survey on the plants found in the Philippines with antidiabetic property/activity was conducted in order to exhaust the natural remedies present in the country that can help in controlling the disease. Thorough and rigid...

glucosidase enzyme inhibition serve as a potent medication in diabetes management and other carbohydrate mediated diseases as they delay glucose release from dietary origin. As a result, postprandial blood glucose is maintained at a lower level [1]. This study aims to isolate compounds from Antidesma bunius Linn. Spreng, an ethnopharmacological pla...

The inhibitors of α –glucosidase serve as a potent medication in diabetes management and other carbohydrate mediated diseases as they delay the release of glucose from dietary origin, and as a result, postprandial blood glucose is maintained at a lower level [1]. This study aims to screen ethnopharmacologically used plants as remedies for diabetes...

Diabetes is one of the ten (10) most prevalent chronic diseases not only in the Philippines but also in the whole world. It is characterized by a high glucose level in the blood (hyperglycemia) and primarily concerned with carbohydrate metabolism. In order to prevent hyperglycemia from affecting other body systems, the postprandial glucose level mu...