Ivan Andreevich Denisov

Siberian Federal University · Department of Biophysics

This article presents a new approach to determining the viscosity of Newtonian fluid. The approach is based on the analysis of the secondary Dean flow in a curved channel. The study of the flow patterns of water and aqueous solutions of glycerin in a microfluidic chip with a U-microchannel was carried out. The advantages of a microfluidic viscomete...

The rapid detection of pollutants in water can be performed with enzymatic probes, the catalytic light-emitting activity of which decreases in the presence of many types of pollutants. Herein, we present a microfluidic system for continuous chemoenzymatic biosensing that generates emulsion droplets containing two enzymes of the bacterial biolumines...

The essential advantages of microfluidic studies are the excellent visualization of the processes of oil displacement from the porous medium model, simple cleaning, and the possibility of the repeated use of the microfluidic chip. The present article deals with the process of oil displacement by suspension flooding using a microfluidic chip, simula...

Microfluidics is a rapidly growing area that provides innovations in biotechnology, medical diagnostics, and life science. Along with the commercial solutions, there are open-source projects aimed to make such technologies more affordable and flexible for end users. In this article, we developed an open-source, low-cost, and easy-to-use microfluidi...

This paper presents the results of testing microfluidic technology for oil displacement problems using cheap and quickly manufactured chips made of polymethylmethacrylate (PMMA) by milling. The oil displacement process from a microfluidic chip simulating a homogeneous porous medium is studied. The microfluidic chip was manufactured by milling of po...

Early detection of pollutants in wastewater, water coming out of treatment facilities, drinking water, and water for agricultural needs is a challenging problem. Effective water quality monitoring requires development of new methods for express detection of pollutants. Enzymes from bioluminescent bacteria can be used for the development of new expr...

Here we discuss the concepts of “biological quantity” and “nominal property” within the framework of the problem of biological measurements based on new specific results of biological analysis using a microfluidic platform and chips developed by our team earlier. It was shown that based on different microfluidic platforms it is possible to develop...

Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhi...

In the present study, we demonstrate the use of a disposable luciferase‐based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve...

A number of approaches have been proposed and tested to transfer enzymatic reactions into the functional elements of microfluidic chips on the example of the bienzyme bioluminescent reaction involving NAD(P)H:FMN-oxidoreductase and luciferase. Measurement of the catalytic activity of these enzymes (under the influence of pollutants) is the basis of...

Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to...

Biosensors for rapid environmental pollution detection can be designed with biomodule based on the bacterial bioluminescent system. Usually this method returns total value of toxicity and does not allow to distinguish pollutants types. Herein we demonstrate the classification of pollutants by the kinetic analysis utilizing artificial neural network...

Dissolution and mixing of flavin mononucleotide (FMN), which activates a luminescent reaction, were considered in various designs of microfluidic chip for pollution analysis of liquid samples. The aim was to determine the velocity mode of fluid flow ensured the uniform distribution of the FMN in the reaction chamber. Simulation of concentration dis...

The electrophysical properties of nanoporous carbon composites consisting of a nanometer-sized pyrolytic carbon matrix and nanodiamonds have been analyzed. It has been shown that the power-law dependence of the electrical resistivity on the thickness of the pyrolytic carbon layer D on a log-log scale has an inflection for D = 1 Å. It has been found...

The electrical and magnetic properties of the nanodiamond composites comprising nanodiamond, pyrolytic carbon, and nanosized pores were studied. The composites are p-type semiconductors and their resistance decreases by 12 orders of magnitude as the pyrocarbon-to-diamond ratio γ increases from 0 to 80 wt %. Evidence for paramagnetic properties of t...

The existence and localization of collective electron states for nanodiamond particles were studied both by solving a one-particle one-dimensional Schr\"odinger equation in the Kronig-Penney potential and by ab initio computations of ground state wavefunctions of diamondoids C78H64, C123H100 and C211H140 at the DFT R-B3LYP/6-31G(d,p) level of theor...

The physical properties of bulk composite materials consisting of nanodiamond, pyrolytic carbon, and nanopores were investigated. Samples were irradiated in a channel of the reactor by fast neutrons (E > 0.5MeV) in ampoules with helium and in an aqueous medium. The dependences of the electrical transport properties of materials with different compo...

Nanodiamond exhibits unpaired electrons in magnetization, EPR, NMR and Auger relaxation. Wave functions and eigenenergies of a bound electron in a nanodiamond crystal have been calculated. It has been proved by using quantum mechanical analysis that unpaired electrons are self-condition of a nanodiamond as a limited crystal according to Tamm theory...

The growing availability of complete genomic sequences from diverse species has brought about the need to scale up phylogenomic analyses, including the reconstruction of large collections of phylogenetic trees. Here, we present the third version of PhylomeDB (http://phylomeDB.org), a public database for genome-wide collections of gene phylogenies (...

The new biotechnical approach to ecological monitoring and medical devices design using biosensor systems based on luciferase enzyme is described. Main scientific, engineering and technological principles of PCR-free nanoparticles-based biobarcode amplification assay are given.

Magnetic properties of composites from nanodiamond and pyrocarbon (NDC) with the factor γ = mass ratio of sp2/sp3 phases have been studied. Solid bulk porous NDC is made as described in [1]. NDC is semiconductor with the electrical conductivity has changed by 12 orders of magnitude that are controlled by the ratio γ from 0 to 80 % [2]. NDC has intr...