Ivan C. Bustamante-Filho

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Verified

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• DVM, MSc, PhD
• Professor (Associate) at Univates

Seminal plasma is comprised of secretions originating from the accessory sex glands, epididymis, and the testis, that contribute to reproductive performance and helps to prevent premature capacitation and to protect sperm against oxidative stress for normal spermatogenesis. Detection of changes in these mechanisms will serve to determine possible c...

Human secondary hypogonadism is associated with impaired testicular function, however, little is known about its impact on sperm epididymal maturation. Endocrine disruption in the epididymis could impair the secretion of key proteins, such as β‐defensins, responsible for spermatozoa maturation during epididymal transit. This study evaluated the seq...

In the present study, we describe the proteome of porcine cauda epididymis fluid and spermatozoa by means of Multidimensional Protein Identification Technology (MudPIT). Ten sexually mature healthy boars were surgically castrated and epididymides were dissected to obtain the cauda epididymal content. Polled protein extracts of cauda epididymal flui...

Osteopontin (OPN) is a multifunctional phosphoprotein that has been linked to fertility in bulls. However, the exact mechanism by which OPN contributes to fertilization is yet unknown. The biotechnological use of OPN in bovine reproduction is promising but some gaps remain unfilled. The present work aimed: (1) to verify if the seminal plasma OPN is...

In vivo, the temperature inside preovulatory follicles of cows is approximately 1ºC lower than rectal temperature. However, standard bovine oocyte in vitro maturation (IVM) protocols use 38.5ºC based on rectal temperature. This study evaluated the effect of reducing IVM temperature to 37.5ºC on the proteomic profile of oocytes compared to the routi...

Canine mammary tumours (CMT) have histological, clinicopathological and molecular resemblances to human breast cancer (HBC), positioning them as viable models for studying the human disease. CMT initiation and progression occur spontaneously in immune‐competent animals, which challenge the suggested limitations of genetically modified mice, also en...

Canine mammary tumors (CMT) demonstrate histological, clinicopathological, and molecular resemblances to Human breast cancer (HBC), positioning them as viable models for studying the human disease. Employing CMT as an HBC model offers substantial advantages, which will bring major improvements to the drug discovery pipeline conventionally based on...

Abstract The present study was conducted to investigate the global proteome of 8-day-old equine blastocysts. Follicular dynamics of eight adult mares were monitored by ultrasonography and inseminated 24 h after the detection of a preovulatory follicle. Four expanded blastocysts were recovered, pooled, and subjected to protein extraction and mass sp...

Canine mammary tumors (CMTs) are the most common neoplasms in female dogs, with a high percentage of malignancy. Surgical excision remains the standard treatment for most cases, except for inoperable or inflammatory neoplasms. Comprehensive global research is needed to understand the nuances of CMTs in different canine populations and develop effec...

Objective Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with seminal plasma removal for cryopreservation in semen parameters. Methods Seminal samples of 26...

Boar seminal plasma (SP) proteins were associated with differences on sperm resistance to cooling at 17°C. However, information about seminal plasma proteins in boars classified by capacity of semen preservation and in vivo fertility remains lacking. Thus, the objective was to evaluate the SP proteome in boars classified by capacity of semen preser...

Sperm cells rely on different substrates to fulfil thei energy demand for different functions and diverse moments of their life. Species specific mechanism involve both energy substrate transport and their utilization: hexose transporters, a protein family of facilitative passive transporters of glucose and other hexose, have been identified in spe...

Over the years, reproductive efficiency in the swine industry has focused on reducing the sperm cell number required per sow. Recent advances have included the identification of subfertile boars, new studies in extended semen quality control, new catheters and cannulas for intrauterine artificial insemination (AI), and fixed‐time AI under commercia...

The study of the protein composition of semen (i.e., spermatozoa and seminal plasma) is not new. However, with development of proteomics technologies, our understanding of the roles of cellular and fluid proteins has expanded enormously. Today, several seminal proteins have already been suggested as biomarkers associated with semen traits (e.g., sp...

The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 25...

The aim was to evaluate the effect of a post-thaw dilution of Rhamdia quelen sperm in 1.1% NaCl (325 mOsm kg-1 ; pH 7.6; 24°C) solution on the quality and reproductive capacity. Sperm from eight males were cryopreservation in nitrogen vapor at-170°C for 18h in 0.25 mL straws in a freezing medium containing 5% fructose, 5% Powdered milk, and 10% met...

The present study was conducted to characterize the major proteome of pre‐implantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2‐6 mm), matured and fertilized in vitro, and cultured until day six. Proteins were extracted separately from three pools of 45 embryos and separately run in SDS‐PAGE. Proteins from...

The use of technologies in assisted reproduction, such as the use of centrifugation, has been reported as a tool that allows separating the best portion of sperm from cryopreserved milt. The main of this work was to compare two separation techniques, differential and density gradient, on thawed Rhamdia quelen milt, based on sperm functionality and...

O estresse oxidativo é caracterizado pelo desequilíbrio entre a produção de espécies reativas de oxigênio e a atuação de sistemas antioxidantes da própria célula e tecido. O espermatozoide de mamíferos apresenta metabolismo oxidativo e por isso é um potencial produtor destas espécies reativas que podem causar danos a estrutura lipídicas, proteínas...

Os primeiros estudos de espermatozoides com citometria de fluxo com espermatozoides iniciaram no final da década de 1970. Com os avanços tecnológicos, hoje contamos com equipamentos com alta sensibilidade e eficiência que, em conjunto com amplo catálogo de sondas fluorescentes, podemos mensurar com alta precisão características celulares. Como exem...

Secondary hypogonadism is a consequence of congenital or acquired diseases that affect the hypothalamus and/or pituitary gland, impairing secretion of gonadotrophin-releasing hormone (GnRH). Androgen deficiency resulting from reduced GnRH secretion is likely to have disrupting effects in epididymal epithelial cells, impairing the sperm maturation p...

Background Hypothermic storage at 5 °C has been investigated as an alternative to promote the prudent use of antibiotics for boar artificial insemination doses. However, this temperature is challenging for some ejaculates or boars. Objective The present study aimed to identify putative biomarkers for semen resistance to hypothermic storage at 5 °C...

Male infertility or sub-fertility is frequently associated with disruption of the hypothalamic-pituitary-testis (HPT) axis events, like secondary hypogonadism. However, little is known how this condition affects the proteomic composition of the epididymal fluid. In the present study, we evaluated the proteomic changes in the cauda epididymal fluid...

Seminal plasma (SP) contributes to sperm physiology and metabolism, prevents premature capacitation, and protects sperm against oxidative stress. In order to evaluate the impact of heat stress in the semen of tropically adapted Brangus breed and in their seminal plasma proteome, we studied the effects of scrotal insulation for 72 h. Semen samples f...

The modern pig industry relies on extensive use of artificial insemination with cooled semen. It is important that semen doses maintain their quality during processing, transport and storage before insemination to guarantee maximum fertility rates. However, ejaculates may respond differently to liquid preservation at 17°C, despite the optimal quali...

In beef cattle breeding systems, mating taaes place during the hottest months of the year and bulls are subject to environmental variations that may interfere ith their fertility and herd reproductive effectiveness. Detection of changes in these mechanisms ill serve to determine possible causes of the decrease in the seminal production. e evaluated...

A adequada analise do sêmen na espécie suína é fator chave para a obtenção da máxima eficiência reprodutiva. Com o avanço tecnológico na avaliação andrológica, a implementação sistemas computadorizados de analise seminal (motilidade espermática, concentração, morfologia e vitalidade celular) já é realidade em muitas centrais de produção de doses in...

The potential of seminal plasma as a relevant source for molecular markers associated with sperm maturation and seminal traits is not well described during heat stress. The goals of this study was to identify bulls with higher resistance to heat stress could improve the reproductive evaluation and management in breeding systems located in tropical...

Cancer is one of the largest causes of morbimortality in the world and due to its prevalence the discovery of novel anticancer drugs in of great importance. The past century brought great advances in the field of plant and microbiology research, with the development of several compounds used in cancer treatment protocols. Many successful anticancer...

The identification of biomarkers associated with seminal traits could aid in the selection of higher quality ejaculates and benefit the swine industry. The objective of this study was to identify boar seminal plasma proteins associated to sperm motility and morphology. Twenty ejaculates from fifteen adult boars from a commercial boar stud were used...

Despite the development of efficient boar semen extenders, there is still room for improvement of new formulas using new molecules that could increase fertilization outcomes and substitute toxic cryoprotectants and antibiotics. The goal of this work was to evaluate if the essential oils from the leaves of Myrrhinium atropurpureum and Cymbopogon cit...

Despite the development of efficient boar semen extenders, there is still room for improvement of new formulas using new molecules that could increase fertilisation outcomes and substitute cryoprotectants and antibiotics. The goal of this work was to evaluate if the essential oils from the leaves of Myrrhinium atropurpureum and Cymbopogon citratus...

Although boar semen productivity is affected by seasonality, its effects are not equal among different regions which raise concerns regarding the profitability of boar stud farms. Therefore, the goals of this study were (i) to evaluate the seasonal effect on semen production in a commercial boar stud farm located in a subtropical climate re- gion a...

The epididymis is a complex organ where spermatozoa acquire motility and ability to fertilize the egg. Epididymal maturation lasts 1 or 2 weeks and exposes the immature spermatozoa to a sequentially modified milieu, promoting intense interactions with secretions by the epididymal epithelium. Sperm surface modifications in response to interactions w...

Hypogonadism is defined as the inadequate gonadal production of testosterone. Low serum testosterone leads to infertility by impairing spermatogenesis and reducing sperm count, however, the impact of hypogonadism in epididymal sperm maturation is poorly understood. From the testis, spermatozoa are transported into the epididymis where they find a s...

Transient endometritis is a normal consequence of breeding and results from uterine contamination with both semen and bacteria. The modulation of the inflammatory response with the use of isoflupredone has been proposed as efficient for the treatment of endometritis by increasing pregnancy rates. The aim o fthe current study was to determine the ef...

Introduction: Type 2 diabetes mellitus (DM2) is the most common metabolic disease. Objective: Toinvestigate the presence of the SstI polymorphism of the C-III apolipoprotein and verify the associationwith the lipid profile in a diabetic sample from Southern Brazil. Materials and methods: Sixty-fivepatients with DM2 were subjected to anthropometric...

Introduction: Physiological mechanisms of scrotal thermoregulation are crucial to allow metabolic functions for normal spermatogenesis. Detection of changes in these mechanisms will serve to determine possible causes in seminal reduction. Seminal plasma is comprised of secretions originating from the accessory sex glands, epididymis, and the testis...

The use of butafosfan in combination with cobalamin modulates many cellular metabolic functions in several species. Its use enhances productive and reproductive performance and reduces stress responses in animals. Despite all these attributes, so far there have been no controlled studies to evaluate the effects of butafosfan and cobalamin on the qu...

This study aims to verify the predominance of contamination with pathogenic microorganisms in dairy herds. In order to validate the initially used methodology, an artificial contamination was conducted in commercially acquired whole UHT milk, with strains of Listeria monocytogenes, Streptococcus agalactiae and Escherichia coli, in final concentrati...

This study aims to verify the predominance of contamination with pathogenic microorganisms in dairy herds. In order to validate the initially used methodology, an artificial contamination was conducted in commercially acquired whole UHT milk, with strains of Listeria monocytogenes, Streptococcus agalactiae and Escherichia coli, in final concentrati...

A growing body of evidence suggests that chaperones are of great importance to control protein quality in spermatogenesis, i.e. the right conformation and function for a given purpose (Van Lith et al., 2007). PDILT, a member of the protein disulfide isomerase (PDI) family is associated to the quality control of important proteins like ADAM3, calmeg...

Milkborne transmission of Shiga toxin- producing Escherichia coli (STEC) has raised considerable concern due to recent outbreaks worldwide and poses a threat to public health. The aim of this study was to develop a sensitive and specific multiplex PCR assay to detect the presence of STEC in bovine raw milk. To identify E. coli (ATCC 25922) contamin...

The acidic Seminal Fluid Protein (aSFP), a 12.9 kDa protein is a maker for bovine semen freezability possibly due to its antioxidant activity and effect on sperm mitochondrial function. However, its precise function on sperm preservation during freezing-thaw is poorly understood. The use of recombinant DNA technology allows new approaches on the st...

The pork is an important source of animal protein for the world, however it’s associated to food poisoning outbreaks. One of the causes of such episodes is the contamination by Escherichia coli, that can be found in the intestinal tract and environment of pigs slaughtered for production of “in natura” and industrialized meat. In the context of food...

Bovine mastitis is the most important udder pathology and have a significant economic impact in the dairy industry. Its etiology is associated to problems with health and milking management. However, there are animals with resistance or susceptibility to mastitis, even when environmental factors are controlled. Recently, some molecular markers were...

Efeito da suplementação nutricional em parâmetros seminais de cachaços com baixa produção de doses de sêmen 25.28 (4,04) meses e peso médio de 300 kg. Os animais receberam 500 g/dia de suplementação nutricional comercial, juntamente com a ração de rotina. Foram avaliados 199 ejaculados pré-suplementação e 215 ejaculados após 1 mês de suplementação....

Efeito da suplementação nutricional em parâmetros seminais de cachaços com baixa produção de doses de sêmen 25.28 (4,04) meses e peso médio de 300 kg. Os animais receberam 500 g/dia de suplementação nutricional comercial, juntamente com a ração de rotina. Foram avaliados 199 ejaculados pré-suplementação e 215 ejaculados após 1 mês de suplementação....

Resumo: O suíno macho em regime de coleta de sêmen possui uma demanda nutricional distinta de animais em sistema de ganho de peso e engorda. A manutenção da espermatogênese requer um aporte energético e proteico em diferentes níveis, bem como possui outros macro e micro elementos de caráter limitantes. Apesar da disponibilização no mercado de suple...

A carne suína representa importante fonte de proteína animal para o mundo, pórem vem sendo associada a surtos de toxinfecção alimentar. Uma das causas destes surtos é a contaminação por Escherichia coli, encontrada no trato intestinal e ambiente dos suínos abatidos para produção de carnes in natura e industrializadas. No contexto de segurança alime...

Sperm maturation in the epididymis is an obligatory process that spermatozoa must suffer in order to acquire the ability to fertilize the egg. During epididymal transit both morphological and biochemical changes occur in the sperm cell and these modifications are tightly controlled by each segment of the epididymal tubule. Protein secretions by epi...

The epididymis is an essential organ for male fertility because is where the post-testicular sperm maturation occurs. Sperm maturation is mediated by protein secretion in the lumen of epididymal tubules, and the result of this process is the transformation of a cell almost inert in a motile gamete with fertilizing capacity. The proteins required fo...

The working life of males in a boar stud is influenced by different factors, but mainly by its semen doses productivity. When a boar presents a significant reduction in doses produced per ejaculate per month, the Stud considers discarding it. Despite the efforts made to guarantee a good nutrition, housing and management, some boars show a reduction...

Milk is the food most consumed worldwide and high nutritional value. Considering the marketing of raw milk and high risk of contamination, this study investigated the presence of Staphylococcus aureus in samples of raw bovine milk, from dairy farm at the state of Rio Grande do Sul (RS), Brazil. We used the technique of Polymerase Chain Reaction (PC...

A mastite bovina é a principal patologia da glândula mamária e a maior causadora de prejuízos na produção leiteira. Sua etiologia é quase sempre relacionada a problemas de manejo sanitário e de ordenha. Entretanto, observa-se a existência de animais com maior ou menor resistência a mastite, mesmo quando fatores ambientais são controlados. Com a fin...

A inseminação artificial é largamente utilizada na suinocultura tecnificada, sendo um dos fatores responsáveis pelos significativos resultados econômicos no setor. Para a obtenção de resultados positivos com a utilização desta tecnologia, é de extrema importância a produção de doses inseminantes (DI) de alta qualidade espermática e livre de microrg...

Cooled-shipped semen is a routine method in horse breeding management. However, when stored over more than 24 h at 5°C, sperm motion characteristics decline significantly. Loss of motility and fertilizing ability has in part been attributed to lipid peroxidation of the sperm plasma membrane caused by the generation of reactive oxygen species during...

Recent studies have demonstrated that PDI family members are involved in a variety oh physiological and pathological processes, acting in catalyzes, isomerization and reduction of protein disulfide bonds, mostly in endoplasmic reticulum (ER) (Benham, 2012). They play an important role in fertility by guaranteeing the correct conformation and functi...

Considering the possible incidence of Listeria monocytogenes in raw foods and their pathogenicity and health risk, this study aimed to compare techniques for extraction of bacterial DNA from milk samples and investigate the presence of L. monocytogenes by Polymerase Chain Reaction (PCR) in raw milk. We tested four different extraction protocols (ge...

The acidic Seminal Fluid Protein (aSFP), a 12.9 kDa protein is a maker for bovine semen freezability possibly due to its antioxidant activity and effect on sperm mitochondrial function. However, its precise function on sperm preservation during freezing-thaw is poorly understood. The use of recombinant DNA technology allows new approaches on the st...

Butaphosphan is a phosphoric acid compound that is available in many countries in combination with vitamin B12. Studies in cattle, horses, swine, broilers, and mice have shown these products to improve general health status by stimulating feed intake, immune system, and digestive function. There is also evidence that butaphosphan and cyanocobalamin...

The aim of this study compared the use of two commercial equine semen extenders supplemented with chicken egg yolk or Rhea americana egg yolk as a lipid source. Ejaculates from six Criollo stallions were visually and microscopically evaluated. Semen samples were divided in two aliquots and diluted in centrifugation extender. Each aliquot was mixed...

Endometrial cytology is a fast and reliable way to assess the uterus status breeding or insemination. Ultrasound examination may detect the presence of uterine luminal fluid (IUF), which suggests susceptibility to persistent endometritis. The aims of this study were (1) to test three different protocols for endometrial cytology sampling and (2) to...

Testis and epididymis are the sites of formation and maturation of spermatozoa and must be well balanced with reactive oxygen species (ROS) and antioxidant agents. The excess of ROS lead to severe cell damage such as membrane lipid peroxidation and DNA fragmentation. On the other hand, an excess of ROS scavengers could inhibit key cell signaling pa...

Cooled-shipped semen is a routine method in horse breeding management. However, when stored over more than 24 h at 5°C, sperm motion characteristics decline significantly. Loss of motility and fertilizing ability has in part been attributed to lipid peroxidation of the sperm plasma membrane caused by the generation of reactive oxygen species during...

Research on molecular markers related to reproductive traits provided new insights on male physiology. The proteomic approach allowed a broader view of expression and function of proteins and their association with several biological phenomena. The freezability-related acidic Seminal Fluid Protein (aSFP) is a 14 kDa protein with remarkable antioxid...

The aim of this work was to determine if an artificial photoperiod program starting in early or late summer can delay the onset of autumn transition. Two experiments were performed in the southern hemisphere. Thirteen and seventeen nonlactating mares were used in the first and second experiments, respectively. In the first experiment, 8 mares were...

Phytomonas wilt or Hartrot is a fatal disease of palm (Arecaceae) species including Cocos nucifera (coconut) and is caused by a phloematic trypanosomatid, a promastigote parasite that inhabits phloem sieve elements of disease palms. In the present work, we described the morphology of the interaction between a phloematic trypanosomatid (Phytomonas s...

Cryopreservation exposes spermatozoa to stressful conditions, leading to reduced cell viability. Several studies propose that overproduction of reactive oxygen species and decreased antioxidant capacity of semen may increase the damaging effects of the technique. The objective of this work was to evaluate the influence of a skim milk-egg yolk based...

Animal reproduction biotechnics are developing day after day. However, almost every applied and studied technology are basicly cellular stressful procedures. Cryopreservation is the most used technic for gamette conservation, although in some species this methodology is not efficiently applied. Apoptosis has been recognized as the one of the major...

During semen cryopreservation, sperm cells were submitted to several deleterious events leading to membrane damage which result in fertility decrease. This study was designed to compare the effects of two freezing techniques (conventional and automated), and the use of two commercial extenders as cryoprotectants (FR-5® and Botu-Crio®) on total and...

Durante o processo de criopreservação de sêmen, os espermatozóides sofrem alguns danos que resultam na diminuição da fertilidade deste. O presente estudo foi realizado com o objetivo de avaliar os efeitos da utilização combinada de duas curvas de congelamento com dois diluentes comerciais (FR-5® e Botu-Crio®) sobre a criopreservação de sêmen eqüino...

The aim of this study was to investigate the presence of persisting mating-induced endometritis in maiden mares compared with the incidence in barren mares and to test the efficiency of a certain intra-uterine treatment. Seventy maiden Thoroughbred mares aged between 3 and 8 years and 69 barren mares were studied. Mares were examined daily by means...

As biotécnicas aplicadas à reprodução animal desenvolvem-se a cada dia. Entretanto, as tecnologias empregadas e estudadas atualmente envolvem processos de grande estresse celular. A criopreservação é a técnica mais utilizada na conservação de gametas, embora esta metodologia ainda não esteja totalmente dominada em algumas espécies. A apoptose vem s...