Ivan Bochev

Institute of Biology and Immunology of Reproduction · Molecular Immunology

Premature ovarian insufficiency (POI) affects 1% of women under 40 years old and represents one of the main causes of infertility in females of childbearing age. POI is a primary ovarian defect resulting from follicular depletion and/or ovarian dysfunction caused by a compromised ovarian somatic cell compartment. Etiologically, ovarian dysfunction...

Objectives: Mesenchymal stem cells (MSCs) exist in almost all tissues. Their unique nature is completed by their immunomodulatory functions, holding promise for the treatment of many diseases. An inflammatory environment precedes the immunosuppressive abilities of MSCs and this study was intended to better understand how umbilical cord MSCs (UCMSC...

Hip replacement is one of the most effective surgical procedures in medicine. Despite the fact that the clinical results of arthroplasty are usually excellent, some implants loosen with time and require revision. To help resolve this problem, research has focused on providing conditions that have a positive biological effect on bone building elemen...

Purpose: Along with comparative investigation of the decidualization potential and IL-6 secretion by fresh and frozen ESCs, we also aimed to evaluate the effectiveness of co-culture systems based on fresh or frozen ESCs in terms of clinical pregnancy rates. Methods: Outcome analysis of a total of 215 IVF cycles with co-culture with fresh or froz...

According to the minimal criteria of the International Society of Cellular Therapy, mesenchymal stem cells (MSCs) are a population of undifferentiated cells defined by their ability to adhere to plastic surfaces when cultured under standard conditions, express a certain panel of phenotypic markers and can differentiate into osteogenic, chondrogenic...

Glioblastoma multiforme (GBM) is the most common and malignant tumor in the central nervous system. One of the contemporary hypotheses postulates that its pathogenesis is associated with the cancer stem cells (CSCs) which originate from mutations in the normal neural stem cells residing in their specific "niches." Simultaneously with its aggressive...

Gonadotropin releasing hormone (GnRH) analogues are used routinely in ART to prevent a premature luteinizing hormone (LH) surge in women un- dergoing controlled ovarian hyperstimulation. The impact of GnRH analogues used in IVF protocols on endometrial function and receptivity is still relatively unclear, aside from their main role in pituitary sup...

The aim of this study was to investigate the proliferation and osteogenic differentiation of mesenchymal stem cells in contact with porous coated titanium implant. Human mesenchymal stem cells (BM-MSCs) were obtained from bone marrow aspirate from the femoral canal during elective hip replacement. The MSCs were isolated and cultured. The resulting...

In general mesenchymal stem cells (MSCs) are considered to be promising agents for replacement of damaged tissues because of their potential to differentiate in a number of cell lineages. Moreover MSCs are potent regulators of the immune response and they are regarded as new and promising means for therapy of autoimmune diseases, graft versus host...

Mesenchymal stem cells (MSCs) are a new and promising tool for therapy of autoimmune disorders. In recent years their possibility to take part in the modulation of the immune response is discussed. The exact mechanisms for immunoregulation realized by MSCs are not clear yet, but interactions with other immunoregulatory cells may be involved in this...

The interfollicular epidermis of human skin functions as the primary barrier between the organism and the environment and provides the first line of defence against environmental damaging agents. The barrier functions of the epidermis are maintained by epidermal stem cells which self-renew and generate daughter cells that undergo terminal different...

Stem cell-based therapies are promising new treatment approaches for a large number of diseases. Since paracrine mechanisms are primarily responsible for the organprotective actions of administered stem cells their conditioned medium has the potency to treat a variety of diseases in humans even without cell transplantation. Our previous work showed...

Maternal immune response to fetal tissues is modified in such way that it favors the development of pregnancy. Human leukocyte antigen (HLA)-G, progesterone and mesenchymal stem cells (MSCs) have been identified as potent immunomodulatory agents in different experimental systems and the interactions between these three factors are studies in this p...

Both mesenchymal stem cells (MSCs) and dendritic cells (DCs) are engaged in the regulation of the immune response parallel to their numerous functions. The main objective of this study was to compare the effects of mesenchymal stem cells isolated from human adipose tissue or human bone marrow on the expression of specific cell surface markers as we...

Maternal immune response to fetal tissues is modified in such a way that it favours the development of pregnancy. HLA-G, progesterone and mesenchymal stem participate in fetomaternal systems and the interactions between these three factors are studied in this paper. We aimed to investigate the effect of progesterone on mesenchymal stem cells expres...

Recently, the interest in endothelial progenitor cells (EPCs) grows because of two possible clinical applications. First, homologous or autologous transplantation of cord blood-derived EPCs may augment postnatal neovascularization in ischemic tissues (i.e., therapeutic vasculogenesis). Second, the fact that transplanted EPCs are incorporated into s...

Mesenchymal stem cells (MSC) have been characterized as multipotent cells which are able to differentiate into several mesodermal and nonmesodermal lineage cells and this feature along with their extensive growth and comprehensive immunomodulatory properties establish them as a promising tool for therapeutic applications. Although bone marrow (BM)...

Mesenchymal stem cells (MSC) have been characterized as multipotent cells which are able to differentiate into several mesodermal and nonmesodermal lineage cells and this feature along with their extensive growth and comprehensive immunomodulatory properties establish them as a promising tool for therapeutic applications, including cell-based tissu...