Ishan Agrawal- Ph.D.
- PostDoc Position at University of Oxford
Ishan Agrawal
- Ph.D.
- PostDoc Position at University of Oxford
Postdoctoral Research Fellow
About
11
Publications
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Publications
Publications (11)
Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymeriza...
Dopamine (DA) is central to neurological functions like motivation, movement and reward (Klein et al. 2019). While its role in diseases like Parkinson’s, Alzheimer’s and schizophrenia are well studied, its role in regulating immune functions have just started to unfold. DA receptors are present on almost all immune cells and they regulate innate an...
Gliomas are the most prevalent primary brain tumors with immense clinical heterogeneity, poor prognosis and survival. Gliomas arise from glial cells and are heavily infiltrated with innate immune cells. In India high-grade gliomas or Glioblastomas (GBM) account for 59.5% of CNS tumours. A key challenge for clinical management of GBM is its highly h...
Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed in...
Microglia are resident innate immune cells of the central nervous system (CNS). Microglia play a critical role during development, in maintaining homeostasis, and during infection or injury. Several independent research groups have highlighted the central role that microglia play in autoimmune diseases, autoinflammatory syndromes and cancers. The a...
NeuroscienceObtaining Human Microglia from Adult Human Brain Tissue doi: 10.3791/61438 Published: August 30, 2020
DOI
Ishan Agrawal1, Shivanjali Saxena1, Preethika Nair1, Deepak Jha2, Sushmita Jha1
1Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, 2Department of Neurosurgery, All India Institute of Medical Sci...
In 1907, Alois Alzheimer observed, as he quoted, development of “numerous fibers” and “adipose saccules” in the brain of his diseased patient Auguste Deter. The neurodegenerative disease became known as Alzheimer’s disease (AD) and is the most common cause of dementia worldwide. AD normally develops with aging and is mostly initiated because of the...
Apoptosis associated speck like protein containing CARD (ASC) is widely researched and recognized as an adaptor protein participating in inflammasome assembly and pyroptosis. It contains a bipartite structure comprising of a pyrin and a caspase recruitment domain (CARD) domain. These two domains help ASC function as an adaptor molecule. ASC is enco...
Hypoxia is a pathological condition known to occur in several diseases including stroke and certain cancers. The development and testing of a portable hypoxia chamber remains a challenge. Hypoxia simulating devices typically consist of a sealed chamber that is placed inside the existing laboratory incubator at 37⁰ C. Existing devices for simulating...
Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed in...
Gliomas are the most prevalent primary brain tumors with immense clinical heterogeneity, poor prognosis and survival. The nucleotide-binding domain, and leucine-rich repeat containing receptors (NLRs) and absent-in-melanoma 2 (AIM2) are innate immune receptors crucial for initiation and progression of several cancers. There is a dearth of reports l...
Questions
Question (1)
I have been doing a western blot with the transfer condition of 350 mA (about 100 V) for 1 hour 10 mins at 4 degree. The transfer works fine when I am doing only one transfer at a time. I wanted to know whether the transfer condition will vary if I do two transfers at a time i.e. transferring proteins from two gels to the membrane in the same apparatus. Thank you.
