Isabelle Jeusette

• Doctor of Veterinary Medicine
• Affinity-Petcare

The objective of this study was to evaluate the effects of ovariectomy (i) and ad libitum feeding (ii) on energy intake, body weight (BW), body composition, thyroid status, leptin and ghrelin plasma concentrations. Four young adult female Beagle dogs were fed a maintenance diet for 6 weeks prior to ovariectomy, then 6 months after. Food allowance w...

The objective of this study was to evaluate, in dogs, the effects of obesity and weight loss on plasma total ghrelin and leptin concentrations. Twenty-four Beagle dogs, 12 control lean and 12 obese dogs of both genders and aged between 1 and 9 years, were used for the experiments. Mean body weight was 12.7+/-0.7 kg for the lean group and 21.9+/-0.8...

The aim of this study was (i) to evaluate the effect of ovariectomy on daily energy requirement in Beagle dogs, and (ii) to evaluate the effects of ad libitum feeding with a high energy diet on energy consumption, body weight gain and blood metabolites in these spayed dogs. Four young adult female Beagle dogs were used. Ovariectomy induced a signif...

OBJECTIVE To determine whether consumption of a single dental treat with specific mechanical properties and active ingredients would provide a 24-hour effect on dental plaque bacteria and halitosis in dogs. ANIMALS 10 dogs of various breeds from a privately owned colony that had received routine dental scaling and polishing 4 weeks before the study...

Objective : To evaluate (1) the effects of three mild stressors on urinary cortisol - a stress marker - in cats and to evaluate (2) the effects on this parameter of a new prescription diet (diet US) with L-tryptophan, lemon balm, oligofructose and fish peptides compared with a control prescription diet (Diet U) with L-tryptophan alone. Methods : T...

Twenty-six cats were fed either a control or an inulin-supplemented diet (0.6%) for six weeks with measurements of fecal short-chain fatty acids (SCFAs) and microbiota. In the second phase, cats were fed their respective diets for another six weeks and were then primo-vaccinated against the feline leukemia virus (FeLV) at days 0 and 21. Serum anti-...

Introduction Escherichia coli is the most common uropathogen in humans, dogs and cats. Dietary consumption of cranberry ( Vaccinium macrocarpon ) is known to be associated with a reduction in uropathogenic E. coli (UPEC) adhesion to human and canine urinary epithelial cell lines, but this has not been shown in cats. Material and Methods Six neuter...

Different starch-to-protein ratios were compared among neutered and spayed domiciled cats. Male and female obese and non-obese cats were fed kibble diets ad libitum for four months high in starch (HS(38%CP): starch 32%, protein 38%; dry-matter basis) or high in protein (HP(55%CP): starch 19%, protein 55%) but similar in energy and fat in a crossove...

Introduction Escherichia coli is the most common pathogen isolated from the urine of dogs with urinary tract infections (UTIs). While there are many studies in humans investigating the potential for the prevention of UTIs by dietary consumption of cranberry, few analogous studies have been carried out in dogs. Material and Methods Eight dogs, four...

The aim of this study was to determine the clinical efficacy of a dental chew with mechanical and chemical properties in 2 toy dog breeds. Eight Yorkshire terriers and 9 Chihuahuas participated in a crossover design trial. Gingivitis, dental plaque, calculus, and volatile sulfur compounds (VSC) in the breath were assessed after 4 weeks and 9 weeks,...

Fecal microbiota evaluation in lean and overweight neutered cats I. Jeusette, A. Salas, M. Compagnucci, Ll. Vilaseca, C. Torre Affinity Petcare, R&D, Barcelona, Spain. Introduction: A previous study has shown the existence of a lean (L) and overweight (OW) phenotype in neutered male and female cats at one year of age, explained at least in part...

Introduction: Subnormal cobalamin concentrations have been described in cats with gastrointestinal disease1. Current cobalamin supplementation protocols in cat are parenteral repeated injections, although a recent publication suggests the effectivity of oral supplementation in cats2. Serum cobalamin concentration does not necessarily reflect cellul...

In developed countries, dogs and cats frequently suffer from obesity. Recently, gut microbiota composition in humans has been related to obesity and metabolic diseases. This study aimed to evaluate changes in body composition, and gut microbiota composition in obese Beagle dogs after a 17-week body weight (BW) loss program. A total of six neutered...

Retrospective study about energy intake, body composition and biochemistry in junior neutered cats with lean or overweight phenotype at one year of age I. Jeusette1, A. Salas1, S. Fernandez2, M. Compagnucci1, Ll. Vilaseca1, A. Tvarijonaviciute3, J. Ceron3, C. Torre1 1Affinity-Petcare, R&D, Barcelona, Spain. 2DOC’S veterinary clinic- Premià, Barce...

Background A link between lipid metabolism and disease has been recognized in cats. Since hepatic lipidosis is a frequent disorder in cats, the aim of the current study was to evaluate liver and plasma lipid dimorphism in healthy cats and the effects of gonadectomy on lipid profiling. From six female and six male cats plasma and liver lipid profile...

Background Research in cats has shown that increased fermentation-derived propionic acid and its metabolites can be used as alternative substrates for gluconeogenesis, thus sparing amino acids for other purposes. This amino acid sparing effect could be of particular interest in patients with kidney or liver disease, where this could reduce the kidn...

Background Feline hepatic lipidosis (FHL) is a common cholestatic disease affecting cats of any breed, age and sex. Both choline deficiency and low hepatic phosphatidylethanolamine N-methyltransferase (PEMT) activity are associated with hepatic lipidosis (HL) in humans, mice and rats. The PEMT expression is known to be upregulated by oestrogens, pr...

Fat digestibility is decreased in old cats for unknown reasons. Subclinical gastrointestinal diseases and pancreatic dysfunction, both related to ageing, can affect food digestibility. The aim of the present study was to elucidate the prevalence of subnormal cobalamin concentration and pancreatic disease in old cats and study the relationship betwe...

The purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. Subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disea...

Fat digestibility was shown to present an age-related decline in cats (Harper, 1998) for unknown reasons. Subclinical gastrointestinal diseases can affect food digestibility. Serum fPLI and vitamin B12 concentration are markers of subclinical pancreatitis or gastrointestinal diseases. The aim of this study was to evaluate food digestibility in appa...

Introduction Feline chronic pancreatitis occurrence increases with age (De Cock et al., 2007). Blood vitamin B 12 has been proposed as a marker of gastroenteric or pancreatic disease in cats (Simpson et al., 2001). The aim of this study was to determine whether a correlation between age and vitamin B 12 deficiency in cats exists and if this could b...

Estrogen inhibition of food intake diminishes with neutering, often resulting in unhealthy weight gain. A potent hop PE, 8‐prenylnaringinen (8PN), may be a suitable source of estrogen‐replacement to stem excessive post‐neutering food intake. Ad libitum‐fed, adult (3.9–4.3 y), neutered males (n=6) of 105–148% of pre‐neuter weight (4.7–7.0 kg) receiv...

The effects of the dietary inclusion of two levels of short-chain fructooligosaccharides (sc-FOS) on weight loss, biochemical parameters and serum haptoglobin concentration were investigated in twelve experimental obese Beagle dogs. Dogs were randomised into two groups and submitted to a weight loss program (WLP): the control group (C) received a c...

Among obesity-associated disorders, low-grade inflammation has been described. The putative therapeutic properties of citrus and curcumin polyphenols could be associated with their anti-inflammatory properties. Two diets supplemented either with hesperidin (0.05 %) and naringin (0.1 %) from citrus extract or with highly bioavailable curcumin from C...

Periodontal disease in cats is highly prevalent, and its aetiology is associated to bacteria located in the subgingival microbiota, being Porphyromonas sp. the most prevalent genus. The conventional technique to sample the subgingival microbiota is the use of cotton swabs over the mucosa and teeth; however the use of subgingival paper points could...

To compare in overweight cats the effects of feeding moderate-energy diets with moderate fat content but with saturated fat (beef tallow), saturated fat plus citrus flavanones, or monounsaturated fat (olive oil) on plasma lipids and urinary F2-isoprostane concentrations. 20 overweight cats with mean+/-SD body weight of 5.2+/-0.2 kg and mean body co...

The objectives of this clinical study were firstly, to assess the effects of breed/genetic group on body composition in dogs using Dual-X-ray absorptiometry (DEXA) as the reference method and secondly, to check, in clinical field conditions, if methods easy to apply in veterinary practice [bioelectrical impedance (BIA), morphometric equations, body...

The putative therapeutic properties of curcuma and citrus could be associated with their antioxidant and anti‐inflammatory properties. Our aim was therefore to examine the effects of citrus or curcuma extract supplementation on body condition and inflammatory state in obese cats. Eight obese cats participated in this study that was conducted as a c...

Polyphenol compounds may explain most of the health-related beneficial effects of plants and vegetables, mainly through their antioxidant properties. The aim of the study was to assess the main changes on leukocyte gene expression of dogs caused by intake of three natural polyphenol-rich extracts and to compare them with caloric restriction. 20 fem...

The objective of this study was to assess the effect of excess body weight (BW) on blood cholesterol, triacylglycerol (TAG), acute phase proteins, and on urinary F2-Isoprostane in healthy cats. Seven lean and 20 overweight cats fed ad libitum with the same food were investigated. The results show that excess BW is associated with increased oxidativ...

The objective of this study was to compare the effects of two diets on weight loss in two groups of seven obese beagles matched for age and gender. The first group received a high-protein diet (45%, 50%, and 5% metabolizable energy [ME] from protein, fat, and carbohydrate, respectively; 4483 kcal ME/kg dry matter [DM]), and the second group receive...

A high antioxidant capacity of plant polyphenols (PPs) has been observed both in vitro and in vivo in humans and experimental animals. The objective of this study was to evaluate the effects of oral PPs on dogs' blood antioxidant status. Twenty adult female beagles were divided into five groups: citric extract (CE), green tea extract (GTE), grape s...

To determine effects of obesity and diet in dogs on plasma lipid and lipoprotein concentrations by assaying plasma leptin and ghrelin concentrations and determining total plasma cholesterol and triglyceride concentrations as well as the concentrations of cholesterol and triglycerides in various lipoprotein classes (ie, very-low-density, low-density...

A family of five privately owned rough collie dogs was referred for corneal lipidosis and also suffered from hypercholesterolaemia. The hypercholesterolaemia was characterised by an increase in the alpha-2 high density lipoprotein-1 band and was due to an increase in the cholesterol content of the very low density lipoprotein, low density lipoprote...

The effects of weight loss on hormonal and biochemical blood parameters were measured monthly [carnitine, creatinine, urea, free T4 (fT4), total T4 (TT4), plasma alkaline phosphatases (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), potassium and total proteins] or bimonthly [cholesterol, triglycerides, non-esterified fatty...

This synthesis aims to summarize lipids metabolism in the dog. The different aetiology and diagnostic of primary or secondary hyperlipidaemia in dog are reviewed. The nutritional treatment and more particularly the use of dietary fibres are discussed.

Obesity and weight loss have been shown to alter thyroid hormone homeostasis in humans. In dogs, obesity is the most common nutritional problem encountered and weight loss is the cornerstone of its treatment. Therefore, it is important to clarify how obesity and weight loss can affect thyroid function test results in that species. The objectives of...

The multi-enzymatic method with constant pH described by Dufour-Etienne et al. (Rec. Méd. Vét. 168, 789-796, 1992) was tested using 17 industrial dry dog foods and two ingredients, sodium caseinate and beef meat, which were used as references. The quantity of sodium hydroxide added to the enzymatic solution was measured after incubation times of 2,...

This paper is the second part of a review dealing with the cat nutrition at different stages of the cycle of life. In the first part, nutrients requirements of adult cat at maintenance were defined. Overweight cat, kitten and older cat are studied in this second synthesis. Optimum and minimum needs of cat at different life stages are compared, and...

This paper is the first of a review in 2 parts dealing with the cat nutrition at different stages of the cycle of life and premium commercial products. The paper presents the energy and nutrients requirements of adult cat at maintenance, the ingredients commonly used in petfood and the flavouring agents. Finally, the study of the composition of var...

This synthesis aims to summarize the specific dietary requirements for prevention or nutritional treatment of feline lower urinary tract diseases and overall urolith's formation. Since aetiologies are diverse and the epidemiological knowledge are changing within the recent year, empirical treatment of the pathology isn't anymore advisable. Symptoma...

This paper aims to present the nutritional requirements of cats suffering from chronic renal failure, the most common condition in renal pathology. Indeed, the concept consisting in a reduced protein content diet is a little out of date. Renal anatomy and function are reviewed as well as the main renal pathologies. Incidence, symptoms, diagnosis an...