Isabelle Dupin

Isabelle Dupin
French Institute of Health and Medical Research | Inserm · Cardio-Thoracic Research Centre of Bordeaux

PhD

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52
Publications
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Publications

Publications (52)
Article
Full-text available
Rhinovirus (RV) infection of the bronchial epithelium is implicated in the vast majority of severe asthma exacerbations. Interestingly, the susceptibility of bronchial epithelium to RV infection is increased in persons with asthma. Bronchial smooth muscle (BSM) remodeling is an important feature of severe asthma pathophysiology, and its reduction u...
Article
Background: Patients with severe asthma show an increase in both exacerbation frequency and bronchial smooth muscle (BSM) mass. Rhinovirus (RV) infection of the bronchial epithelium (BE) is the main trigger of asthma exacerbations. Histological analysis of biopsies shows that a close connection between BE and hypertrophic BSM is a criterion for se...
Article
Résumé La bronchopneumopathie chronique obstructive (BPCO) est une maladie respiratoire caractérisée par une inflammation chronique et un remodelage bronchique, entraînant une limitation des débits d’air. Plusieurs études plaident en faveur d’un rôle néfaste des lymphocytes T CD8⁺ (LT CD8⁺) dans la physiopathologie de la maladie, néanmoins les méca...
Article
Introduction Chronic obstructive pulmonary disease (COPD) is a respiratory disease with chronic inflammation, in which CD8⁺ T cells play a key role. Since circulating and tissue fibrocytes are associated with mortality and bronchial obstruction, respectively, we investigated whether tissue fibrocytes can interact with CD8⁺ T cells, and whether the...
Article
Introduction Fibrocytes are circulating monocytes-derived cells, able to migrate to injured organs in order to differentiate into myofibroblasts-like cells. We have previously shown that their densities are increased in the lungs of Chronic Obstructive Pulmonary Disease (COPD) patients and associated to worse lung function (Eur Respir J 2019 54: 18...
Article
Introduction Asthma is a very frequent airway disease affecting 6 to 20% of the population of western European countries. The disease is characterized by an increase of bronchial smooth muscle (BSM) mass correlated with the severity of asthma. Recent clinical investigations have shown a link between BSM mass and the exacerbations rate. Exacerbation...
Article
Introduction Severe asthmatic patients are characterized by an increase in both exacerbation frequency and bronchial smooth muscle (BSM) mass. Rhinovirus (RV) infection of the bronchial epithelium (BE) is the main trigger of asthma exacerbations. A decreased distance between BE and BSM mass is associated with the severity of asthma. We hypothesized...
Article
Introduction Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease with chronic inflammation, in which CD8⁺ T cells play a key role. Since circulating and tissue fibrocytes are associated with mortality and bronchial obstruction, respectively, we investigated whether tissue fibrocytes can interact with CD8⁺ T cells, and whe...
Article
Background Bronchial smooth muscle (BSM) remodelling in asthma is related to an increased mitochondrial biogenesis and enhanced BSM cell proliferation in asthma. Since (i) mitochondria produce the highest levels of cellular energy and (ii) fatty acid beta-oxidation is the most powerful way to produce ATP, we hypothesized that, in asthmatic BSM cell...
Article
Full-text available
Background: Animal models and, in particular, mice models, are important tools to investigate the pathogenesis of respiratory diseases and to test potential new therapeutic drugs. Lung function measurement is a key step in such investigation. In mice, it is usually performed using forced oscillation technique (FOT), negative pressure-driven forced...
Article
The remodelling mechanism and cellular players causing persistent airflow limitation in chronic obstructive pulmonary disease (COPD) remain largely elusive. We have recently demonstrated that circulating fibrocytes, a rare population of fibroblast-like cells produced by the bone marrow stroma, are increased in COPD patients during an exacerbation....
Article
Full-text available
: Chronic Obstructive Pulmonary Disease (COPD) represents the 3rd leading cause of death in the world. The underlying pathophysiological mechanisms have been the focus of extensive research in the past. The lung has a complex architecture, where structural cells interact continuously with immune cells that infiltrate into the pulmonary tissue. Both...
Preprint
Full-text available
Background: The remodeling mechanism and cellular players causing persistent airflow limitation in chronic obstructive pulmonary disease (COPD) remain largely elusive. We have recently demonstrated that circulating fibrocytes, a rare population of fibroblast-like cells produced by the bone marrow stroma, are increased in COPD patients during an exa...
Article
Fibrocytes are circulating cells that have fibroblast properties. They are produced by the bone marrow stroma, and they move from the blood to injured organs using multiple chemokine pathways. They exhibit marked functional and phenotypic plasticity in response to the local tissue microenvironment to ensure a pro-inflammatory or a more resolving ph...
Article
Background: Increase of bronchial smooth muscle (BSM) mass is a crucial feature of asthma remodeling. The mechanisms of such an increased BSM mass are complex but involve enhanced mitochondrial biogenesis, leading to increased proliferation of BSM cells in asthmatic patients. The major tumor suppressor protein p53 is a key cell regulator involved...
Article
Rationale: Increased bronchial smooth muscle (BSM) mass is a key feature of airway remodeling that classically distinguishes severe from non-severe asthma. Proliferation of BSM cells involves a specific mitochondria-dependent pathway in severe asthmatic patients. However, BSM remodeling as well as mitochondrial biogenesis have not been examined in...
Article
Background: Chronic obstructive pulmonary disease (COPD) is characterized by peribronchial fibrosis. The chronic course of COPD is worsened by recurrent acute exacerbations. Objective: The aim of the study was to evaluate the recruitment of blood fibrocytes in patients with COPD during exacerbations and, subsequently, to identify potential mecha...
Article
Full-text available
Background: Increase of bronchial smooth muscle (BSM) mass is a crucial feature of asthma remodeling. The mechanisms of such an increased BSM mass are complex but involve enhanced mitochondrial biogenesis, leading to increased proliferation of BSM cells in asthmatic patients. The major tumor suppressor protein p53 is a key cell regulator involved i...
Article
Introduction Asthma is a frequent respiratory disease characterized by bronchial hyperresponsiveness, inflammation and remodeling. Bronchial remodeling corresponds to structural modification of the bronchial wall. Increase of smooth muscle mass is a crucial feature of such a remodeling in asthma. Indeed, it correlates with the decrease in lung func...
Article
Introduction Increased bronchial smooth muscle mass is one of the key structural features of severe asthma. In adults, asthmatic airway smooth muscle cells (ASMC) demonstrate greater mitochondrial biogenesis associated with an increase in ASMC proliferation rate vs. non-asthmatic ASMC. However, to the best of our knowledge, there is no evidence tha...
Article
Introduction Asthma is a very frequent airway disease that affects 5 to 20% of the whole population worldwide. Whereas the mortality of asthma has been dramatically decreased within the last 10 years, uncontrolled asthma still induces major consequences on morbidity, quality of life, and economic burden. Asthma control determinants have been associ...
Article
Full-text available
Axon pathfinding is controlled by guidance cues that elicit specific attractive or repulsive responses in growth cones. It has now become clear that some cues such as Netrin-1 can trigger either attraction or repulsion in a context-dependent manner. In particular, it was recently found that the repellent Slit1 enables the attractive response of ros...
Article
Full-text available
The nucleus is generally found near the cell center; however its position can vary in response to extracellular or intracellular signals, leading to a polarized intracellular organization. Nuclear movement is mediated by the cytoskeleton and its associated motors. While the role of actin and microtubule cytoskeletons in nuclear positioning has been...
Article
The nucleus is generally found near the cell center; however its position can vary in response to extracellular or intracellular signals, leading to a polarized intracellular organization. Nuclear movement is mediated by the cytoskeleton and its associated motors. While the role of actin and microtubule cytoskeletons in nuclear positioning has been...
Article
Full-text available
Rationale: Severe asthma is a major public health issue throughout the world. Increased bronchial smooth muscle (BSM) mass, a characteristic feature of airway remodeling in severe asthma, is associated with resistance to high-intensity treatment and poor prognosis. In vitro, the Ca(2+)-channel blocker gallopamil decreased the proliferation of BSM...
Article
Rationale: Asthma is a frequent airway disease, and asthma control determinants have been associated with indoor allergen sensitization. The most frequent allergens are house dust mites (HDM), which act in vivo on the bronchial epithelial layer. Severe asthma has also been associated with bronchial remodeling and more specifically with increased m...
Article
Full-text available
The precise wiring of the nervous system relies on processes by which axons navigate in a complex environment and are guided by a concerted action of attractive and repulsive factors to reach their target. Investigating these guidance processes depends critically on our ability to control in space and time the microenvironment of neurons. The imple...
Article
Nerve growth cones (GCs) are chemical sensors that convert graded extracellular cues into oriented axonal motion. To ensure a sensitive and robust response to directional signals in complex and dynamic chemical landscapes, GCs are presumably able to amplify and filter external information. How these processing tasks are performed remains however po...
Article
Nerve growth cones (GCs) are chemical sensors that convert graded extracellular cues into oriented axonal motion. Ensuring a sensitive and robust GC response to directional signals requires the ability to amplify and filter external gradients. However, our knowledge of how these signal processing tasks are performed remains sparse. Here we present...
Article
The nucleus is the largest organelle in the cell and its position is dynamically controlled in space and time, although the functional significance of this dynamic regulation is not always clear. Nuclear movements are mediated by the cytoskeleton which transmits pushing or pulling forces onto the nuclear envelope. Recent studies have shed light on...
Article
Full-text available
The localization of the nucleus is precisely regulated, and defects in nuclear positioning are observed in diseases such as lissencephaly, cerebellar ataxia and dysplasia. We show here that cytoplasmic intermediate filaments are essential players in actin-dependent positioning of the nucleus. The actin retrograde flow is relayed by a flow of interm...
Article
Full-text available
Control of cell polarity is crucial during tissue morphogenesis and renewal, and depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell-cell interactions, we show that in the absence of other polarizing cues, cell-cell contacts are the main regulator of nucleus and centrosome p...

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