Isabel Pividori

Isabel Pividori
Autonomous University of Barcelona | UAB · Department of Chemistry

38.31
 · 
Grade in Biochemistry, PhD in Chemistry
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Introduction
María Isabel Pividori is tenured Professor at the Autonomous University of Barcelona (UAB), Catalonia, Spain. She leads a research team on bioanalytical chemistry and biosensing, particularly focused on the design of electrochemical biosensing devices and emerging technologies appropriate at community and primary-care level for clinical diagnosis and food safety in low resource settings.
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Projects (3)
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Project
Infectious diseases are responsible for hundreds of thousands of deaths and an enormous burden of morbidity worldwide. The accurate identification of patients requiring treatment remains a major issue to disease control, since the burden of infectious disease would reduce if appropriate diagnostic tests were more widely available in the developing world as well as in low-resource settings in the developed world. This proposal addresses the development of new platforms for diagnostics to meet the United Nations Millennium Development Goals related with global health for reducing the burden of disease. The characteristics of the new platforms that will be designed are summarized under the acronym ASSURED defined by the WHO (Affordable; Sensitive; Specific; User-friendly; Rapid and Robust; Equipment-free; and, Delivered to those who need it. The targets will be selected among the major problems facing the global health, including pathogenic bacteria occurring in food outbreaks, in order to ensure safety in food and water supplies in low resources settings, as well as major global infection disease in low-incomes countries, such as AIDS, malaria and dengue. The technology derived of this project will be also transferable to other novel biomarkers related with developed countries for personalized medicine.
Project
CeliFast is a rapid diagnostic test (RDT) for celiac disease (CD). CeliFast merges all the competitive advantages of the diagnostic test currently in the market: point of care, rapid, user-friendly, quantitative, and highly predictive. Celifast consists on a rapid in vitro diagnostic kit. The technological core of CeliFast, which is already patented, relies in a novel diagnostic biomarker for CD based on deamidated gliadin peptides coupled to magnetic particles and their integration on a magneto-actuated platform. FUNDING ACKNOWLEDGEMENTS This project was funded by LLAVORS 2014 (Grants for Innovative Projects with potential for the Productive Sector) in 50% by the European Regional Development Fund of the European Union under the Operational Programme FEDER Catalonia 2014-2020. Aquest projecte ha estat cofinançat en el marc de la convocatòria d’ajuts per a projectes innovadors amb potencial d’incorporació al sector productiu (Llavor 2014) en un 50 % pel Fons Europeus de Desenvolupament Regional de la Unió Europea en el marc del Programa operatiu FEDER de Catalunya 2014-2020.
Research
Research items (110)
Data
Supplementary information regarding the article: "Electrochemical Sensor for Alkaline Phosphatase as Biomarker for Clinical and in vitro Applications" DOI: 10.1016/j.snb.2018.10.105
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The present work evaluates the action of nitroreductase enzyme immobilized on Tosylactivated magnetic particles (MP-Tosyl) on three disperse dyes which contain nitro and azo groups. The dyes included Disperse Red 73 (DR 73), Disperse Red 78 (DR 78), and Disperse Red 167 (DR 167). The use of a magnet enabled the rapid and easy removal of the immobil...
Article
Full-text available
Alkaline phosphatase (ALP) is an important biomarker of bone diseases clinically measured in blood as a routine assay by means of optical read-outs, which when compared with electroanalytical methods present lack of miniaturization, slow response and high cost. Thus, electrochemical ALP sensors have arisen as a tool to develop disposable low cost d...
Article
This work addresses a novel, rapid and cost-effective approach for the electrochemical sensing of scombrotoxin (histamine) in fish based on magnetic molecularly imprinted polymer (magnetic-MIP). The histamine magnetic-MIP was synthesized by the core-shell method using histamine as a template, and 2-vinyl pyridine as functional monomer. The magnetic...
Article
Up to date, tissue regeneration of large bone defects is a clinical challenge under exhaustive study. Nowadays, the most common clinical solutions concerning bone regeneration involve systems based on human or bovine tissues, which suffer from drawbacks like antigenicity, complex processing, low osteoinductivity, rapid resorption and minimal accele...
Article
Interferon-γ is a proinflammatory cytokine, and its production is related with effective host defense against intracellular pathogens. Therefore, the level of interferon-γ is considered a good biomarker for intracellular infections. It is also useful for the assessment, treatment progression and follow-up of non-communicable diseases, including can...
Article
Disperse Orange 37 (DO37) is an efficient azo dye for dyeing synthetic textile materials owing to its resistance to degradation that may also be harmful to humans as DO37 is not entirely eliminated in wastewater treatment. In this paper, we demonstrate that DO37 is bleached by reduced glutathione (GSH) in a reaction catalyzed by glutathione-s-trans...
Article
Interferon-γ is a proinflammatory cytokine, and its production is related with effective host defense against intracellular pathogens. Therefore, the level of interferon-γ is considered a good biomarker for intracellular infections. It is also useful for the assessment, treatment progression and follow-up of non-communicable diseases, including can...
Article
This study addresses the rational design of a magnetic molecularly imprinted polymer (magnetic-MIP) for the selective recognition of the hormone levothyroxine. The theoretical study was carried out by the density functional theory (DFT) computations considering dispersion interaction energies, and using the D2 Grimme's correction. The B97-D/def2-SV...
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Full-text available
The determination of 1-chloro-2,4-dinitrobenzene (CDNB) was used as a proof-of-concept to a simple analytical practical configuration applying magnetic molecularly imprinted particles (mag-MIPs). Mag-MIPs were captured from an emulsion by a home-made magneto-sensor (where a small magnet was entrapped by a graphite-epoxy composite) and then, this se...
Article
Full-text available
Magnetic molecularly imprinted polymer (mag-MIP) were developed for the analysis of ametryn (4-N-ethyl-6-methylsulfanyl-2-N-propan-2-yl-1,3,5-triazine-2,4-diamine), a popular triazine herbicide. Iron oxide (Fe3O4) nanoparticles were synthetized, and then modified with tetraethoxysilane (TEOS) and 3-mercaptopropyltrimethoxysilane (MPS). Then MIPs' p...
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Full-text available
Magnetic molecularly imprinted polymers (MMIPs) have become a research hotspot due to their two important characteristics: target recognition and magnetic separation. This paper presents the preparation, characterization, and optimization of an MMIP for the preconcentration of disperse red 73 dye (DR73) and its subsequent efficient degradation by p...
Article
Molecularly imprinted polymers (MIPs) in combination with magnetic nanoparticles, in a core@shell format, were studied for selective detection of 1-chloro-2,4-dinitrobenzene (CDNB), a powerful allergenic substance. Magnetic nanoparticles were prepared by the co-precipitation method and mixed with oleic acid (OA). This material was then encapsulated...
Article
This work addresses the design of an Ebola diagnostic test involving a simple, rapid, specific and highly sensitive procedure based on isothermal amplification on magnetic particles with electrochemical readout. Ebola padlock probes were designed to detect a specific L-gene sequence present in the five most common Ebola species. Ebola cDNA was ampl...
Chapter
Molecular imprinting has become one of the most effective techniques in developing biomimetic material and, within these approaches, molecularly imprinted polymers (MIP) have proven their potential in synthetic recognition. Their advantages, when compared to biological material (like enzymes and antibodies), include high chemical and thermal stabil...
Article
The analysis of dyes in hair samples can provide useful information for forensic purposes. The present work describes a novel method for the extraction of dye from a hair sample and its determination using a composite electrode to pre-concentrate carboxyl-functionalized magnetic nanoparticles (CFMP) employed to collect the dye in solution, hence in...
Article
Due to the increasing need of rapid tests for application in low resource settings, WHO summarized their ideal features under the acronym ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, Delivered to those who need it). In this work, two different platforms for the rapid and simultaneous testing of the food...
Article
Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is their stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic p...
Article
The Acquired Immune Deficiency Syndrome (AIDS) affect the life of millions of people around the world. Although rapid and low cost screening tests are widely available for the diagnosis of HIV infection, the count of CD4+ T lymphocytes remains a drawback in the areas mostly affected by the HIV, being this control imperative for assessing the deteri...
Article
Conductance was measured in two different double strands DNA (both with 20 bases), the more conducting poly(dG)-poly(dC) (ds-DNAc) and the less conducting poly(dA)-poly(dT) (ds-DNAi), by means of Electrochemical Capacitance Spectroscopy (ECS). The use of ECS approach, exemplified herein with DNA nanowires, is equally a suitable and a time-dependent...
Chapter
Infectious diseases are responsible for hundreds of thousands of deaths and an enormous burden of morbidity worldwide. Although most of these major infectious diseases are treatable, the accurate and rapid identification of the pathogens remains a major issue to disease control, since the incidence of infectious disease would be reduced if appropri...
Article
This paper addresses a sensitive method for the detection of mycobacteria in hemodialysis water samples based on a magneto-actuated immunoassay with optical readout. In this approach, micro (2.8 μm) sized magnetic particles were modified with an antibody against the lipoarabinomannan (LAM) located in the mycobacterial cell wall. The system relies o...
Article
Simultaneous detection of Salmonella enterica, Listeria monocytogenes and Escherichia coli based on triple-tagging multiplex PCR and electrochemical magneto genosensing on silica magnetic particles is reported. A set of tagging primers were selected for the specific amplification of yfiR (375bp), hlyA (234bp) and eaeA (151bp), being one of the prim...
Article
Full-text available
Some diuretic substances are controlled and monitored by the World Anti-Doping Agency as prohibited substances for use by athletes, such as triamterene (TRT). Thus, this work describes a voltammetric method based on graphite-epoxy composite electrode modified by tosyl-functionalized magnetic particles (GECE/MPs-To) for determination of TRT diuretic...
Article
A magneto-genosensing approach for the detection of the three most common pathogenic bacteria in food safety, such as Salmonella, Listeria and Escherichia coli is presented. The methodology is based on the detection of the tagged amplified DNA obtained by single-tagging PCR with a set of specific primers for each pathogen, followed by electrochemic...
Article
The counting of CD4(+) T lymphocytes is a clinical parameter used for AIDS diagnosis and follow-up. As this disease is particularly prevalent in developing countries, simple and affordable CD4 cell counting methods are urgently needed in resource-limited settings. This paper describes an electrochemical magneto-actuated biosensor for CD4 count in w...
Article
Nanocomposite materials are being increasingly used in biosensing applications as they can significantly improve biosensor performance. Here we report the use of a novel impedimetric genosensor based on gold nanoparticles graphite-epoxy nanocomposite (nanoAu-GEC) for the detection of triple base mutation deletion in a cystic-fibrosis (CF) related h...
Article
Full-text available
Infectious diseases affect the daily lives of millions of people all around the world, and are responsible for hundreds of thousands of deaths, mostly in the developing world. Although most of these major infectious diseases are treatable, the early identification of individuals requiring treatment remains a major issue. The incidence of these dise...
Article
Full-text available
This review discusses the current state of the art of Salmonella detection. In this perspective, emphasis is given to the recent developments in biosensors, in particular to electrochemical immunosensors, genosensors and phagosensors. Different aspects of the biosensors development has been summarised and discussed in detail. The integration of new...
Article
Full-text available
An amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized enzyme and a magnetic graphite-epoxy composite (m...
Article
A new electrochemical immunosensor has been developed to detect paraquat (PQ) pesticide residues in food samples. The immunosensor presented uses electrochemical nanoprobes prepared by labelling the PQ specific antibodies with CdS nanoparticles (CdSNP) and antigen biofunctionalized magnetic μ-particles. Electrochemical measurements are performed us...
Article
This work explores the use of the phage P22 in a phagomagnetic immunoassay for the rapid detection of Salmonella. The covalent attachment of wild-type phages was performed on two different magnetic carriers: carboxyl-activated magnetic nanoparticles (300 nm) and tosyl-activated magnetic microparticles (2.8 μm). The bacteria were captured and precon...
Article
A magneto immunofluorescence assay for the detection of anti-transglutaminase antibodies (ATG2) in celiac disease was developed. The ATG2 were recognized by transglutaminase enzyme immobilized on the magnetic beads and then the immunological reaction was revealed by antibodies labeled with peroxidase. The fluorescent response of the enzymatic react...
Article
This work presents the first study and development of an electronic tongue analysis system for the monitoring of nitrogen stable species: nitrate, nitrite and ammonium in water. The electronic tongue was composed of an array of 15 potentiometric poly(vinyl chloride) membrane sensors sensitive to cations and anions plus an artificial neural network...
Article
A new electrochemical magnetoimmunosensor (EMIS) has been developed for the screening of residues of sulfonamide antimicrobials in honey samples. The immunosensor is able to detect up to ten different sulfonamide congeners at levels below the action points established in some European countries (25 μg kg−1) after a hydrolysis step in which the sulf...
Article
An electrochemical magneto immunosensor for the detection of low concentrations of paraquat (PQ) in food samples has been developed and its performance evaluated in a complex sample such as potato extracts. The immunosensor presented uses immunoreagents specifically developed for the recognition of paraquat, a magnetic graphite-epoxy composite (m-G...
Article
An electrochemical magneto immunosensor for the detection of anti-transglutaminase antibodies (ATG2) in celiac disease was developed. The immunological reaction is performed on magnetic beads (MBs) as a solid support in which the transglutaminase enzyme (TG2) is covalently immobilized (TG2-MB) and then ATG2 were revealed by an antibody labeled with...
Article
An approach based on the electrochemical detection of the horseradish peroxidase enzymatic reaction by means of square wave voltammetry was developed for the determination of phenolic compounds in environmental samples. First, a systematic optimization procedure of three factors involved in the enzymatic reaction was carried out using response surf...
Article
This paper addresses the use of bacteriophages immobilized on magnetic particles for the biorecognition of the pathogenic bacteria, followed by electrochemical magneto-genosensing of the bacteria. The P22 bacteriophage specific to Salmonella (serotypes A, B, and D1) is used as a model. The bacteria are captured and preconcentrated by the bacterioph...
Article
A new electrochemical immunosensor has been developed to detect sulfonamide antibiotic residues in food samples. The immunosensor presented uses immunoreagents specifically developed for the broad recognition of the sulfonamide antibiotic family, a graphite composite electrode (GEC), biofunctionalized magnetic μ-particles and electrochemical nanopr...
Conference Paper
Full-text available
A very simple and rapid method for the detection of S. enterica is reported. In this approach, the bacteria were captured and preconcentrated with magnetic particles through an immunological reaction. A second polyclonal antibody labeled with peroxidase was used for the electrochemical immunosensing based on a magneto-electrode. Different nano and...
Article
An electrochemical magneto biosensor for the rapid determination of biotin in food samples is reported. The affinity reaction was performed on streptavidin-modified magnetic microbeads as a solid support in a direct competitive format. The biotinylated horseradish peroxidase enzyme (biotin-HRP) competes with free biotin in the sample for the bindin...
Chapter
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Article
The electrochemical detection for horseradish peroxidase-cosubstrate-H(2)O(2) systems was optimized. o-Phenilendiamine, phenol, hydroquinone, pyrocatechol, p-chlorophenol, p-aminophenol and 3,3'-5,5'-tetramethylbenzidine were evaluated as cosubstrates of horseradish peroxidase (HRP) enzyme. Therefore, the reaction time, the addition sequence of the...
Article
Full-text available
This work reports the application of a Bio-Electronic Tongue (BioET) system made from an array of enzymatic biosensors in the analysis of polyphenols, focusing on major polyphenols found in wine. For this, the biosensor array was formed by a set of epoxy-graphite biosensors, bulk-modified with different redox enzymes (tyrosinase and laccase) and co...
Article
Gliadin is a constituent of the cereal protein gluten, responsible for the intolerance generated in celiac disease. Its detection is of high interest for food safety of celiac patients, since the only treatment known until now is a lifelong avoidance of this protein in the diet. Therefore, it is essential to have an easy and reliable method of anal...
Conference Paper
This work reports the application of a BioElectronic Tongue (BioET) in the analysis of polyphenols. For this, a voltammetric BioET formed by an array of epoxy‐graphite biosensors, bulk‐modified with different redox enzymes, aimed to the simultaneous determination of different polyphenols was used. Departure information was the set of voltammograms...
Article
Magneto immunoassay-based strategies for the detection of Plasmodium falciparum histidine-rich protein 2 (HRP2) related to malaria are described for the first time by using magnetic micro- and nanoparticles. The covalent immobilization of a commercial monoclonal antibody toward the HRP2 protein in magnetic beads and nanoparticles was evaluated and...
Article
A novel conducting silver nanocomposite (nanoAg-GEC) material for electrochemical sensing of substituted anilines based on silver nanoparticles is presented. For the first time, the in situ growth of silver hexacyanoferrate nanoparticles (nanoAgHCF) on the surface of the silver nanocomposite electrodes was achieved. The surface morphology of the na...
Article
The growth of zinc hexacyanoferrate (ZnHCF) hybrid film on the surface of graphite-epoxy composite (GEC) electrodes was demonstrated by cyclic voltammetry. Surface morphology of the hybrid film was investigated by using scanning electron microscopy. The effect of the type of monovalent cations on the redox behaviour of hybrid film was also studied....
Article
This paper describes a model for the analysis of the quality of teaching and learning in higher education. Empirically validated through an extensive student‐focused survey, this model puts forward a concept of quality that challenges that which is commonly used in institutional analysis. Research indicates that quality, rather than being a simple...
Article
We report on the integration of micro and nanostructured materials in biosensing devices to obtain analytical nano-bio-systems with improved performance. The simplest case of electrochemical biosensing presented here is based on graphite microparticles to obtain a graphite-epoxy composite. The integration of the bioreceptor within the composite pro...
Article
In this work we report the use of an impedimetric genosensor for the model detection of H1N1 swine flu correlated DNA sequence. An oligonucleotide DNA probe, complementary to the target H1N1 virus sequence, was immobilized onto the electrode surface by covalent binding. Two different protocols, i.e. direct hybridization with the DNA target and a sa...
Article
First results are presented of a new voltammetric electronic tongue which employs modified epoxy-graphite electrodes. This analytical tool has been applied to qualitative wine analysis, performing the classification of wine varieties, as well as recognition of the oxygenation effect. In the same way, studies related to the detection of some defects...
Article
Full-text available
SUMMARY: A highly sensitive assay for rapidly screening-out Mycobacterium bovis in contaminated samples was developed based on electrochemical genosensing. The assay consists of specific amplification and double-tagging of the IS6110 fragment, highly related to M. bovis, followed by electrochemical detection of the amplified product. PCR amplificat...
Article
A very simple and rapid method for the detection of Salmonella in milk is reported. In this approach, the bacteria are captured and preconcentrated from milk samples with magnetic beads through an immunological reaction. A second polyclonal antibody labeled with peroxidase is used as serological confirmation with electrochemical detection based on...
Article
A rapid and sensitive method for the detection of food pathogenic bacteria is reported. In this approach, the bacteria are captured and preconcentrated from food samples with magnetic beads by immunological reaction with the specific antibody against Salmonella. After the lysis of the captured bacteria, further amplification of the genetic material...
Article
Full-text available
p> Preguntarse por la calidad del aprendizaje en Educación Superior (E.S.) no es una cuestión nada simple. Como toda apreciación de naturaleza evaluadora, fundamentalmente depende de los referentes empleados en hacerlo y del modo cómo se realiza. Ello convierte a dicha noción en un concepto complejo y controvertido debido a las distintas aproximaci...
Article
Double-tagged DNA coming from PCR amplification of a Salmonella spp. sample was detected by an electrochemical impedimetric genosensor based on avidin bulk-modified graphite-epoxy biocomposite (Av-GEB). The double-tagging PCR strategy provided the amplicon with both biotin and digoxigenin (DIG) moieties. The immobilization of the double-tagged DNA...
Article
A novel electrochemical immunosensing strategy for the detection of antibodies to tissue transglutaminase (tTG) in human serum is presented. The proposed immunosensor consists of the immobilization by physical adsorption of tTG from guinea pig liver on graphite-epoxy composite (GEC) electrodes. After the reaction with the human serum (containing th...
Article
A novel material for electrochemical biosensing based on rigid conducting gold nanocomposite (nano-AuGEC) is presented. Islands of chemisorbing material (gold nanoparticles) surrounded by nonreactive, rigid, and conducting graphite epoxy composite are thus achieved to avoid the stringent control of surface coverage parameters required during immobi...
Article
An immunoassay-based strategy for folic acid in vitamin-fortified milk with electrochemical detection using magneto sensors is described for the first time. Among direct and indirect competitive formats, best performance was achieved with an indirect competitive immunoassay. The immunological reaction for folic acid (FA) detection was performed, fo...
Article
Disposable magnetic DNA sensors using an enzyme-amplified strategy for the specific detection of a gene related to the Enterobacteriaceae bacterial family, based on the coupling of streptavidin-peroxidase to biotinylated lacZ gene target sequences, has been developed. A biotinylated 25-mer capture probe was attached to streptavidin-modified magneti...
Article
A very sensitive assay for the rapid detection of pathogenic bacteria based on electrochemical genosensing has been designed. The assay was performed by the PCR specific amplification of the eaeA gene, related with the pathogenic activity of Escherichia coli O157:H7. The efficiency and selectivity of the selected primers were firstly studied by usi...
Article
This chapter presents a procedure to perform an in situ polymerase chain reaction (PCR) amplification of the IS200 insertion sequence of Salmonella spp. with double labeling of the amplicon on magnetic bead primers and to quantify the double-labeling amplification products growth on magnetic beads by using an electrochemical strategy based on magne...
Article
This chapter presents a method for the purification of the specific anti-sulfonamide antibody, for the construction of a magneto graphite–epoxy composite (m-GEC) electrode, for the immobilization of the specific anti-sulfonamide antibody on Tosyl-modified magnetic beads, and for the quantification of sulfonamide antibiotics in milk based on its imm...
Article
Graphite–polymer composites (GEC)-based platforms are useful and versatile transducer materials for electrochemical DNA genosensing of amplicon food pathogens. GEC materials present a low non-specific adsorption either for DNA probes or enzyme labels. They do not require blocking steps to minimize the non-specific adsorption on the free sites of th...
Article
Traditional microbial screening methods have insufficient sensitivities to meet new regulations and classical physicochemical techniques, such as chromatographic methods and mass spectrometry, which are often precluded because of the level of experience, skills, and cost involved. The development of biosensors is a growing area, in response to the...
Article
This chapter presents a procedure (i) to perform a polymerase chain reaction (PCR) amplification of the IS200 insertion sequence of Salmonella spp, (ii) to construct a graphite–epoxy composite (GEC) electrode, and (iii) to identify Salmonella spp. by using an electrochemical strategy based on GEC electrode. Amplification of the salmonella genome in...