Isabel Natalia Sierra Garcia

Isabel Natalia Sierra Garcia
University of Aveiro | UA · Centre for Environmental and Marine Studies (CESAM)

PhD.

About

36
Publications
16,320
Reads
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782
Citations
Introduction
PhD in Genetics and Molecular Biology focused in environmental microbiology. My research goals are directed to understanding metabolic diversity of microbial communities in natural or man-made environments and their abilities to adapt in response to environmental changes.
Additional affiliations
March 2009 - August 2018
Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA) - UNICAMP
Position
  • Research Associate
April 2014 - March 2015
Newcastle University
Position
  • PhD Student -internship
January 2006 - January 2007
Corporación para Investigaciones Biológicas
Position
  • Research Assistant

Publications

Publications (36)
Article
Full-text available
Microorganisms have shown their ability to colonize extreme environments including deep subsurface petroleum reservoirs. Physicochemical parameters may vary greatly among petroleum reservoirs worldwide and so do the microbial communities inhabiting these different environments. The present work aimed at the characterization of the microbiota in bio...
Article
Full-text available
Current knowledge of the microbial diversity and metabolic pathways involved in hydrocarbon degradation in petroleum reservoirs is still limited, mostly due to the difficulty in recovering the complex community from such an extreme environment. Metagenomics is a valuable tool to investigate the genetic and functional diversity of previously uncultu...
Article
Metagenomics has been successfully applied to isolate novel biocatalysts from diverse environments and functional screening has been used as the primary technique to identify clones carrying the desired traits. This work aimed at the construction and screening of a metagenomic library as a source of potential hydrocarbon biodegraders. A library of...
Article
Full-text available
Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more li...
Article
Aims This study investigated the bacterial communities in the rhizosphere of two traditional Portuguese olive cultivars, Cobrançosa and Negrinha de Freixo in relation to soil properties. Additionally, we aimed to isolate and identify bacteria with potential for biocontrol and other plant growth promoting traits from these rhizosphere communities....
Article
Full-text available
Plant-growth-promoting bacteria (PGPB) have beneficial effects on plants. They can promote growth and enhance plant defense against abiotic stress and disease, and these effects are associated with changes in the plant metabolite profile. The research problem addressed in this study was the impact of inoculation with PGPB on the metabolite profile...
Article
Aims Climate change is endangering olive groves. Farmers are adapting by exploring new varieties of olive trees and examining the role of microbiomes in plant health. The main objectives of this work were to determine the primary factors that influence the microbiome of olive trees and to analyze the connection between the rhizosphere and endospher...
Preprint
Full-text available
This study assessed the impact of inoculation with plant growth-promoting bacteria (PGPB) on stress-resistance and growth-promoting metabolites in Salicornia europaea L. across controlled and field conditions. Salicornia europaea seeds, inoculated with Brevibacterium casei EB3 and Pseudomonas oryzihabitans RL18, were grown in controlled laboratory...
Article
Full-text available
Plant growth-promoting bacteria (PGPB) can revolutionize sustainable agriculture by improving crop yields and resilience in the face of climate change and soil degradation. However, one of the challenges of using PGPB is identifying strains that can colonize and establish beneficial relationships with plant hosts and microbiomes. This study examine...
Article
Full-text available
Photodynamic inactivation (PDI) is an emerging therapeutic approach that can effectively inactivate diverse microbial forms, including vegetative forms and spores, while preserving host tissues and avoiding the development of resistance to the photosensitization procedure. This study evaluates the antifungal and sporicidal photodynamic activity of...
Article
The utilization of lignocellulosic biomasses for biogas production can slow down the biogas generation process. This study aimed to develop an effective inoculant composed of cattle manure rumen for the anaerobic digestion of maize silage. Four different proportions of cattle manure and rumen were tested as inoculants (IN) in this biomass. Biogas c...
Article
Aim: We aim at understanding the effect of domestication on the endophytic microbiome and metabolome of Salicornia europaea and collecting evidence on the potential role of microbial populations and metabolites in the adaptation of plants to different ecological contexts (wild vs crops). Methods and results: Samples were collected from a natural...
Article
Aim: The objective of the work was to assess the effect of biostimulation with selected plant growth-promoting bacteria on growth and metabolite profile of Salicornia europaea. Methods and results: S. europaea seeds were inoculated with different combinations of plant growth-promoting bacteria Brevibacterium casei EB3, Pseudomonas oryzihabitans...
Article
Full-text available
Antimicrobial photodynamic therapy (aPDT) has been explored as an innovative therapeutic approach because it can be used to inactivate a variety of microbial forms (vegetative forms and spores) without causing significant damage to host tissues, and without the development of resistance to the photosensitization process. This study assesses the pho...
Article
Full-text available
The idea of agricultural sustainable in the EU is based on, both minimizing interference with the soil system as well as diversifying crop rotation what relates to the limited cultivation system (changed from plow to no-plowing tillage) as well as organic fertilization is often abandoned. Taking above into account, our goal was determined of the st...
Article
Antimicrobial photodynamic inactivation (PDI) has been explored as an innovative therapeutic and phytosanitary approach because it can be used to inactivate microbial vegetative forms and spores, without significant development of resistance to photosensitization and collateral damage to host tissues. The objective of this study was to assess the p...
Article
Full-text available
Citrus are economically important fruit crops to which infectious diseases like citrus canker caused by Xanthomonas citri subs. citri, citrus variegated chlorosis caused by Xylella fastidiosa, “huanglongbing” associated with the presence of Candidatus liberibacter species, anthracnose caused by Colletotrichum gloeosporioides and citrus black spot c...
Article
Full-text available
Microorganisms inhabiting subsurface petroleum reservoirs are key players in biochemical transformations. The interactions of microbial communities in these environments are highly complex and still poorly understood. This work aimed to assess publicly available metagenomes from oil reservoirs and implement a robust pipeline of genome-resolved meta...
Preprint
Full-text available
Microorganisms inhabiting subsurface petroleum reservoirs are key players in biochemical transformations. The interactions of microbial communities in these environments are highly complex and still poorly understood. This work aimed to assess publicly available metagenomes from oil reservoirs and implement a robust pipeline of genome-resolved meta...
Article
Full-text available
The fungus Lasiodiplodia theobromae is one of the main causal agents of trunk canker and dieback of grapevine. The objective of this work was to evaluate the efficiency of photodynamic inactivation (PDI) of L. theobromae with synthetic and natural photosensitizers and irradiation with either sunlight or artificial photosynthetically active radiatio...
Article
Full-text available
A Correction to this paper has been published: https://doi.org/10.1038/s41587-021-00898-4.
Article
Full-text available
Root−associated microbial communities play important roles in the process of adaptation of plant hosts to environment stressors, and in this perspective, the microbiome of halophytes represents a valuable model for understanding the contribution of microorganisms to plant tolerance to salt. Although considered as the most promising halophyte candid...
Article
Full-text available
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
Full-text available
Petroleum is a very complex and diverse organic mixture. Its composition depends on reservoir location and in situ conditions and changes once crude oil is spilled into the environment, making the characteristics associated with every spill unique. Polycyclic aromatic hydrocarbons (PAHs) are common components of the crude oil and constitute a group...
Article
Microbial biodegradation of hydrocarbons in petroleum reservoirs has major consequences in the petroleum value and quality. The identification of microorganisms capable of in-situ degradation of hydrocarbons under the reservoir conditions is crucial to understand microbial roles in hydrocarbon transformation and the impact of oil exploration and pr...
Article
Full-text available
Methanogenesis is an ancient metabolism of key ecological relevance, with direct impact on the evolution of Earth’s climate. Recent results suggest that the diversity of methane metabolisms and their derivations have probably been vastly underestimated. Here, by probing thousands of publicly available metagenomes for homologues of methyl-coenzyme M...
Article
The taxonomic and functional diversity of three different biological reactors (fluidized bed reactor, FBR; up-flow anaerobic sludge blanket reactor, UASB; and expanded granular sludge bed reactor, EGSB) used for commercial laundry wastewater treatment was investigated using metagenome shotgun sequencing. Metagenomes were sequenced on the Illumina H...
Article
Microbial degradation of petroleum is a worldwide issue, which causes physico-chemical changing in its compounds, diminishing its commercial value. Biosurfactants are chemically diverse molecules, that can be produced by several microorganisms, and can enable the microbial access to hydrocarbons. In order to investigate both microbial activities, f...
Article
Full-text available
Indigenous bacteria isolated from a crude oil sample from a deep water reservoir in the Pampo Sul Oilfield (Campos Basin-RJ, Brazil) were previously classified as strains of B. pumilus. However, their enzymatic activities with fluorogenic probes and rates of petroleum biodegradation were completely different. Some of the bacteria depleted n-alkanes...
Article
Full-text available
Colombia is considered one of the countries with the greatest biological diversity, but very little of this diversity has been explored to identify biologically active substances. Secondary bacterial metabolites may exhibit activity to plant and animal pathogens and represent alternative biotechnologies for industry. The objective of this study was...
Article
Metagenomics has been successfully applied to isolate novel biocatalysts from diverse environments and functional screening has been used as the primary technique to identify clones carrying the desired traits. This work aimed at the construction and screening of a metagenomic library as a source of potential hydrocarbon biodegraders. A library of...
Article
Full-text available
Although diverse bacteria capable of degrading petroleum hydrocarbons have been isolated and characterized, the vast majority of hydrocarbon-degrading bacteria, including anaerobes, probably remain undiscovered [1]. This limitation imposed by conventional culturing techniques can be circumvented by the metagenomic approach, which is based on cultur...

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