Isabel Lara

Isabel Lara
Universitat de Lleida | UDL · Department of Chemistry

BSc; MSc; PhD

About

118
Publications
48,798
Reads
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2,560
Citations
Introduction
Isabel Lara works at the Department of Chemistry of the Universitat de Lleida (UdL). Isabel's research work is focused on ripening and postharvest biology & technology of fruits.
Additional affiliations
October 2002 - December 2017
Universitat de Lleida
Position
  • Associate Professor (tenured)
Description
  • Research + teaching activity
December 1999 - October 2002
Universitat de Lleida
Position
  • Lecturer (full time)
Description
  • Research + teaching activity
March 1996 - November 1999
Universitat de Lleida
Position
  • Lecturer (part time)
Description
  • Research + teaching activity
Education
September 1993 - June 1995
Autonomous University of Barcelona
Field of study
  • Biotechnology
October 1992 - November 1999
Autonomous University of Barcelona
Field of study
  • Plant Physiology
September 1990 - September 1991
Autonomous University of Barcelona
Field of study
  • Plant Physiology

Publications

Publications (118)
Article
The composition and structure of fruit surface tissues have a noticeable influence on the postharvest storage potential of fruit, inasmuch as they behave as a barrier against drying, chemical attack, mechanical injuries and microbial infection. The cuticle is made of cutin, a biological insoluble polyester, embedded in an impermeable wax complex, a...
Article
Full-text available
Cuticles are plant structures, composed mostly by lipidic layers, synthesized by nonwoody aerial plant organs and deposited on the surface of outer epidermal cell walls. Although its significance has been often disregarded, cuticle deposition modifies organ chemistry, influences mechanical properties, and plays a central role in sensing and interac...
Article
Ripening-related firmness loss shortens considerably the storage potential of sweet cherry (Prunus avium L.), thus limiting postharvest handling, transportation, and commercialisation. The biochemical mechanisms underlying this process in cherries are not fully understood, and the mechanisms operating in a given fruit may be not extrapolated to a d...
Article
Full-text available
Olive (Olea europaea L.) growing has outstanding economic relevance in Spain, the main olive oil producer and exporter in the world. Fruit skin properties are very relevant for fruit and oil quality, water loss, and susceptibility to mechanical damage, rots, and infestations, but limited research focus has been placed on the cuticle of intact olive...
Article
Fruit cuticles influence greatly postharvest quality of produce. Yet, few published studies have addressed changes in cuticle composition and related gene expression from a postharvest perspective, particularly for fruit species within the Rosaceae family. Because physical treatments have proved effective in improving postharvest potential in some...
Article
Full-text available
The olive tree (Olea europaea L.) has been cultivated around the Mediterranean basin since ancient times, ‘Arbequina’ being one of the most widely grown varieties. To improve the knowledge on ripening-related firmness changes in olive fruit, cell wall metabolism was studied in irrigated and rain-fed ‘Arbequina’ olives grown at ‘Les Garrigues’, a Pr...
Article
Full-text available
Fresh blueberries are prone to softening and dehydration during postharvest, which limits their competitiveness when reaching the final markets. Commercial cultivars 'Duke' and 'Brigitta' exhibit contrasting softening patterns. Although 'Duke' berries usually show higher firmness levels at harvest as compared to 'Brigitta', they display higher soft...
Cover Page
Full-text available
The ripening process encompasses many molecular, biochemical and physiological alterations in the course of which fleshy fruits acquire those sensory attributes which render them edible. These modifications do not stop after harvest, and hence these produce are highly perishable commodities. A variety of postharvest strategies are applied in an att...
Article
Full-text available
The allure of the vedute, those picturesque depictions of buildings, monuments and towns which were so fashionable during the xviii and xix centuries, goes well beyond their aesthetic value. They represent a paramount source of knowledge, since they do not provide ex-ternos information uniquely, namely on the appearance of a particular building or...
Article
Full-text available
Olive oil is an essential part of the so-called “Mediterranean diet”, purportedly one of the healthiest gastronomic traditions in the world. The wax content in olive oil is regulated under European Union directives, and it is used as a purity parameter for extra-virgin and virgin olive oils. The wax profile may also help the characterization of mon...
Article
The production of olive (Olea europaea L.) is very important economically in many areas of the world, and particularly in countries around the Mediterranean basin. Ripening-associated modifications in cell wall composition and structure of fruits play an important role in attributes like firmness or susceptibility to infestations, rots and mechanic...
Article
Barrier properties of the hydrophobic plant cuticle depend on its physicochemical composition. The cuticular compounds vary considerably among plant species but also among organs and tissues of the same plant and throughout developmental stages. As yet, these intraspecific modifications at the cuticular wax and cutin level are only rarely examined....
Cover Page
Full-text available
Editorial on the Research Topic "Physiological, Molecular and Genetic Perspectives of Chilling Tolerance in Horticultural Crops"
Article
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The plant cuticle is an extracellular barrier that protects the aerial, non-lignified parts of plants from the surrounding environment, and furthermore plays important functions in organ growth and development. The role of the cuticle in post-harvest quality of fruits is a topic currently driving a lot of interest since an increasing bulk of resear...
Article
The fungus Penicillium digitatum is the causal agent of the citrus green mould, the major postharvest diseases of citrus fruit. Lesions on the surface of infected fruits first appear as soft areas around the inoculation site, due to maceration of fruit. The macerating activity has been associated with pectinases secreted by the fungus during infect...
Article
Full-text available
Information on cell-wall metabolism during ripening of intact fruit of olive (Olea europaea L.) is scarce, despite the impact of cell-wall properties on fruit susceptibility to mechanical damage, infestations or rots. In this work, ‘Arbequina’ fruit were picked and classified into five maturity groups according to external colour. Cell-wall materia...
Article
Full-text available
Fresh Chilean blueberries take in average 20-50 days to reach overseas markets, so a better knowledge of their postharvest behavior would help maintaining their quality for longer periods. Quality of highbush blueberries (Vaccinium corymbosum L. ‘Duke’ and ‘Brigitta’) was assessed at six stages based on color: 100% green (100G), 75% green + 25% pin...
Article
The plant cuticle, protecting against uncontrolled water loss, covers olive (Olea europaea) fruits and leaves. The present study describes the organ-specific chemical composition of the cuticular waxes and the cutin and compares three developmental stages of fruits (green, turning and black) with the leaf surface. Numerous organ-specific difference...
Chapter
The cuticle is a mainly lipophilic barrier, which covers and waterproofs all the nonwoody aerial organs of the plant, including fruits. However, in addition to protecting plant organs against transpirational water loss, the cuticle exerts a range of major impacts on surface properties. In the case of fruit, four major aspects of quality modulated b...
Article
Full-text available
The cuticular layer plays an important role in protecting fruits against water loss and invasive microorganisms and insects, both during on-tree development and postharvest storage. There is also experimental evidence that cuticle composition and structure may be a relevant factor accounting for firmness and other textural attributes. Waxes are imp...
Article
Full-text available
‘Sweetheart’ cherries (Prunus avium L.) have sweet flavour, bright red colour and a distinctive heart shape, which contributes to their wide commercial acceptance. They display also comparatively high firmness and long shelf-life potential. Enzyme-catalysed disassembly of cell walls has been generally targeted as the main factor accounting for ripe...
Article
Full-text available
‘Somerset’ is a dark-red, sweet cherry (Prunus avium L.) cultivar displaying remarkable firmness levels, with concomitantly longer shelf-life potential in comparison to other cultivars. It is generally accepted that fruit firmness depends mainly on the composition, structure and interconnections among cell wall polysaccharides. However, the biochem...
Article
Full-text available
Fresh blueberries are very susceptible to mechanical damage, which limits postharvest life and firmness. Softening and susceptibility of cultivars " Duke " and " Brigitta " to developing internal browning (IB) after mechanical impact and subsequent storage was evaluated during a 2-year study (2011/2012, 2012/2013). On each season fruit were careful...
Article
This work aimed at evaluating the effects of sous-vide cooking parameters, such as time and temperature and their interactions, on textural attributes of ‘Mondial Gala’ and ‘Granny Smith’ apple cultivars. For this, different response surface methodology-based models were developed. This methodology proved a suitable means for the assessment of chan...
Poster
Full-text available
Olive (Olea europaea L.) growing in Spain has outstanding economic relevance. The properties of these fruit are largely affected by internal and external factors such as genotype, maturity stage, cultural practices and environmental conditions. The ripening process of olive fruit is poorly understood, and the biochemical mechanisms underlying impor...
Article
Full-text available
http://fst.sagepub.com/content/21/6/416.abstract The biochemical processes underlying firmness loss of sweet cherry (Prunus avium L.) fruit are poorly understood. Studies on cell wall metabolism of sweet cherry have been generally undertaken during on-tree development or at harvest maturity, while published reports on postharvest changes are scarc...
Article
This research focuses on the effect of maturity stage and storage conditions on quality and consumersatisfaction of ‘Big Top®’ nectarines. At harvest time, fruit were graded in three categories according tothe IADindex (index of absorbance difference = A670− A720) based on Vis spectroscopy. Physicochemicalparameters (soluble solids concentration, t...
Article
Full-text available
Two sweet cherry cultivars ('New Star' and 'Sweet Heart') were chosen to explore the impact of passive modified atmosphere packaging on the eating quality and health-promoting properties of fruit. Packaged and unpackaged fruits were stored at 0 ºC for 15 or 30 days, followed by zero or three days at 20 ºC, after which the analyses were undertaken....
Article
Full-text available
Morus nigra L. (black mulberry) and Morus alba L. (white mulberry) display high concentrations of health-promoting compounds, particularly phenolics. However, no published studies have addressed the changes in the content of phenolic compounds during frozen storage, a widely used form of preservation of these fruit in the Turkish countryside. This...
Conference Paper
Se cosecharon melocotones ‘October Sun’ en madurez comercial y se trataron con aire caliente (50 ºC, 45 min) o CO2 (30 kPa, 48 h), tras lo cual se conservaron a 0 ºC durante 14 días, seguidos de 5 días a 20 ºC para simular su período de comercialización. Se aislaron enzimáticamente las cutículas a partir de la piel del fruto, y se analizó su compos...
Conference Paper
Full-text available
‘Celeste’ cherry fruit were dipped in 3% (w/v) CaCl2 (2 min, room temperature) and then kept for 14 days at 0 °C, followed by 3 days at 20 ºC to simulate shelf life. Calcium treatment significantly decreased firmness loss and decay incidence, but did not affect weight loss. After storage, levels of the hemicellulose-containing cell wall fraction in...
Conference Paper
Full-text available
Se aislaron muestras de cutícula de cerezas ‘Somerset’ recolectadas en su punto óptimo de madurez y conservadas 28 días a 0 °C, y se analizó su composición en ceras y monómeros de cutina. En el momento de cosecha, la cutícula del fruto contenía un 23% y un 41% de ceras y cutina, respectivamente, siendo los ácidos triterpenoides y los monómeros de t...
Article
Cuticle composition and structure may be relevant factors affecting the storage potential of fruits, but very few studies have analyzed fruit cuticle composition from a postharvest perspective. In this work, the chemical composition of waxes and cutin (major cuticular components) was analyzed in cuticle samples isolated from 'Celeste' and 'Somerset...
Article
Although postharvest quality of fruit produce is greatly impacted by cuticle composition, structure and properties, very few published studies have analyzed fruit cuticle composition from a postharvest perspective. In this work, the chemical composition of waxes and cutin, major cuticular components, was analyzed in fruit cuticle samples isolated f...
Article
Full-text available
Purpose of review: This paper reviews studies on the effects of preharvest spray treatments on the postharvest quality and storage potential of fruits, with the objective of summarising the main effects in each case and identifying major topics requiring further research. Findings: The literature survey shows that most of the studies on preharvest...
Article
Para evitar el rápido deterioro de las cerezas en la fase poscosecha es conveniente aplicar tratamientos que frenen la maduración del fruto, y, en consecuencia, que ayuden a mantener su calidad y contenido en compuestos bioactivos durante el almacenamiento y distribución comercial. En el presente trabajo se ha pretendido comprobar la eficacia de la...
Article
Full-text available
Sweet cherries are very perishable, owing to rapid softening rates associated to significant deterioration of fruit. Ripening-related firmness loss is generally attributed to enzyme-catalysed modifications in cell wall composition and structure, but some experimental evidence suggests that non-enzymic mechanisms may be also involved in the process....
Chapter
Full-text available
La maduración de frutos engloba numerosos procesos fisiológicos controlados muy estrictamente a nivel genético, que conducen a una serie de modificaciones en las propiedades físicas y organolépticas del fruto (color, sabor, olor, textura) que, al ser captadas por los sentidos del consumidor, determinan la sensación producida en éste. Desde el punto...
Article
Full-text available
Los mecanismos enzimáticos de degradación de las paredes celulares, que resultan en cambios en la firmeza, la textura y la calidad general del fruto durante su vida post-cosecha, han sido históricamente el objetivo preferente de los estudios sobre estos procesos. No obstante, el desensamblaje de esta estrucutra extracelular parece depender asimismo...
Article
Full-text available
In this work, ‘Rich Lady’ peaches were picked at commercial maturity, stored at 2°C and 92% RH under air or CA (3 kPa O2:10 kPa CO2) for 3 or 15 days, and subsequently kept in air 1 day at 7°C to simulate refrigerated transport. After cold storage, samples were placed at 20°C, and fruit firmness, cell wall composition and some cell wall-degrading e...
Article
Full-text available
The emission of aroma volatile compounds and the activity of some related enzymes were monitored throughout fruit maturation of ‘Rich Lady’ peaches (Prunus persica L. Batsch) with the general purpose of studying the development of the ability to produce aroma volatiles, and of identifying which enzymes may act as control points for the ripening-rel...
Article
Full-text available
‘Rich Lady’ peaches were harvested at commercial maturity and stored at 20, 12, 4 or -0.5°C for up to three weeks, with the general purpose of broadening the understanding of storage temperature influence on the development of fruit flavour, a major quality attribute. Stored fruit were analysed weekly for the emission of aroma volatile compounds, a...
Article
Full-text available
This work assessed the effects of harvest date, storage conditions and poststorage ripening period on fruit quality and aroma emission by ‘Belletardie®’ peach. Fruits were harvested on two dates (18th September, 21st September) and stored in air or controlled atmosphere (3 kPaO2/10 kPaCO2). After three weeks of storage at 0°C plus 0 and 7 days of r...
Article
Full-text available
In order to monitor ripening-related cell wall modifications, samples of the ‘Snow Queen’ nectarine cultivar were picked periodically around the commercial harvest date. Yields of insoluble cell wall materials (CWM) in the flesh tissue decreased significantly during the sampling period in parallel to increased yields of the soluble fraction, indica...
Conference Paper
Full-text available
Commercialisation and shelf life of peach (Prunus persica L. Batsch) is largely limited by weight loss, decay and high softening rates. These alterations are connected to rapid firmness loss after harvest, which leads to high susceptibility to mechanical damage and infections, and restricts storage potential and marketing possibilities. In this stu...
Article
Full-text available
‘Golden Reinders’ apples harvested at commercial maturity were dipped in 2% (w/v) CaCl2 and stored for 4 or 7 months at 1°C and 92% RH under air. Cell wall composition and some related enzyme activities were determined thereafter in order to assess the effects of calcium treatment on fruit firmness and cell wall metabolism throughout the commercial...
Article
Full-text available
In this work, samples of both intact and cracked ‘Snow Queen’ nectarines were commercially harvested in order to elucidate if there were differences in cell wall composition and some cell wall-related enzyme activities. Results showed that the amount of cell wall material was similar in both cases. In contrast, yields of fractions containing covale...
Article
Over two subsequent seasons, emission of volatile compounds, consumer acceptability and quality parameters were analyzed in“Fuji” apples. In both seasons, apples were harvested at the same maturity stage and stored for 19 or 30 weeks at 1C and 92% relative humidity under an ultralow oxygen (ULO) atmosphere (1% O2 + 1% CO2) or under an ULO atmospher...
Article
Full-text available
Dado que la mayor parte de la producción de manzana está destinada a su frigoconservación durante períodos relativamente largos, los frutos suelen cosecharse antes de alcanzar la plena madurez, con objeto de conseguir niveles de firmeza superiores y por tanto una mejor aptitud para su manipulación y conservación a largo plazo. Desafortunadamente, e...
Article
Volatile compounds emitted by apple (Malus×domestica Borkh.) fruit change during maturation and ripening, and hence maturity stage at harvest is a key factor determining fruit aroma. In this work, production of aroma volatile compounds and some related enzyme activities were monitored weekly in ‘Golden Reinders’ apples during 7 weeks prior to comme...
Article
‘Golden’ is the most cultivated apple group in Europe. In recent years, new mutants have emerged which improve both aspects of production and sensory attributes. In this work, emission of volatile compounds and the activity of lipoxygenase system–related enzymes in ‘Golden Reinders®’ apples were analysed after 19 and 30 weeks under ultra low oxygen...
Article
Calcium treatment and storage under ultra-low oxygen (ULO) conditions are common post-harvest practices aimed at delaying ripening-related softening of apple (Malus × domestica Borkh.) fruit, but the biochemical mechanisms underlying these effects have not been determined conclusively to date. In this study, commercially mature ‘Golden Reinders’ ap...
Article
To improve apple firmness during the postharvest chain, detailed knowledge of the biochemistry underlying ripening-related cell wall disassembly, a very complex event, is required. Apple softening is reportedly mediated by calcium loss from the middle lamella, and accordingly calcium applications are expected to preserve fruit firmness. In this wor...
Article
All efforts to improve fruit quality are rewarded when consumers are satisfied after tasting the fruit. Apples are often stored under controlled atmosphere conditions to preserve them over time, but this frequently results in a loss of flavor. The aim of this work, which was based on two seasons, was to evaluate the influence of a period of short-t...
Article
The effects of aminoethoxyvinylglycine (AVG; ReTain®) and 1-methylcyclopropene (1-MCP; SmartFresh™) on the biosynthesis of aroma volatiles and overall quality in 'Delbarde Estivale', an early apple variety, are reported for the first time. Apple fruit were treated with AVG 3 weeks before commercial harvest, following standard procedures. 1-MCP was...
Article
Full-text available
The effects of aminoethoxyvinylglycine (AVG; ReTain®) and 1-methylcyclopropene (1-MCP; SmartFresh™) on the biosynthesis of aroma volatiles and overall quality in 'Delbarde Estivale', an early apple variety, are reported for the first time. Apple fruit were treated with AVG 3 weeks before commercial harvest, following standard procedures. 1-MCP was...
Article
Full-text available
Softening of peach (Prunus persica L. Batsch) fruit is generally rapid at ambient temperature, which limits considerably the commercial life of the produce. Modifications in the cell wall are believed to underlie changes in fruit firmness and texture. In this work, the effects of controlled atmosphere (CA) storage and 1-methylcyclopropene (1-MCP) o...
Article
Apple (Malus × domestica Borkh.) fruit intended for long-term storage are frequently harvested commercially before becoming fully ripe, often resulting in poor aroma development. Since postharvest calcium dips have proved effective for the enhancement of flavor-related volatile esters after cold storage of apples, this study was undertaken in order...
Article
Peach fruit is highly perishable, which drastically restricts storage potential and marketing possibilities. Although aroma is a very important attribute for sensory quality of peach, post-harvest procedures, aimed at extending commercial availability of fruit, have focused preferentially on other quality aspects. In this work, we were interested i...
Article
Full-text available
‘Fuji Kiku-8’ apples, harvested at commercial maturity, were dipped in calcium chloride (2% w/v) and stored at 1°C and 92% RH for 4 or 7 months under air plus 7 days at 20°C. The calcium concentration was higher in treated fruit, showing that the treatment was effective in introducing calcium into the tissues. In general, calcium treatment led to e...
Article
Full-text available
‘Fuji Kiku-8’ and ‘Golden Reinders’ apples, harvested at commercial maturity, were dipped in calcium chloride (2% w/v) and stored at 1°C and 92% RH for 4 or 7 months under air. The calcium concentration was higher in treated fruit, showing that the treatment was effective in introducing calcium into the tissues. A number of sensory attributes were...
Article
Full-text available
The aim of this study was to assess the effect of AVG- and 1-MCP treatment on the postharvest behaviour of ‘Delbarde Estivale’ apple kept at room temperature, with special emphasis on the biosynthesis of odor volatiles. Fruits were harvested at the proper time, and subjected to AVG (ReTain®) treatment 3 weeks before harvest date. Another lot of fru...