Isabel Carrasquinho

Instituto Nacional de Investigação Agrária e Veterinária (INIAV) | INRB · Unidade de Sistemas Agrários e Desenvolvimento

Cork oak (Quercus suber L.) is an ecologically and economically important evergreen tree species native to the Mediterranean region and widespread in southwest Europe and northwest Africa. An improved genome assembly of cork oak using a combination of Illumina and PacBio sequencing is presented in this study. The assembled genome contains 2351 scaf...

Background and Aims. In ancient grapevine varieties, the experimental design of field trials is crucial to providing a reliable evaluation of quantitative traits. The main purposes of this study are to demonstrate the benefits of the resolvable row-column design (RCD) for quantifying intravarietal variability and performing polyclonal selection and...

The work was carried out in two areas of the clonal orchard - Area I, with 4 hectares, and Area II, with 3 hectares - installed between 1970 and 1980. In Area I, 286 trees from 43 clones were identified for resin; in Area II, 284 trees from 36 clones were identified. The dendrometric variables of the trees were measured, the resin from the 2021 cam...

Pine wilt disease (PWD) is a severe environmental problem in Eastern Asia and Western Europe, devastating large forest areas and causing significant economic losses. This disease is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, a parasitic migratory nematode that infects the stem of conifer trees. Here we review what is curren...

Purpose of Review Recognizing that in the context of global change, tree genetic diversity represents a crucial resource for future forest adaptation, we review and highlight the major forest genetics research achievements of the past decades in biodiversity-rich countries of the Mediterranean region. For this, we conducted a bibliometric analysis...

Pinewood nematode (PWN, Bursaphelenchus xylophilus) is the causal agent of pine wilt disease (PWD), which severely affects Pinus pinaster stands in southwestern Europe. Despite the high susceptibility of P. pinaster, individuals of selected half-sib families have shown genetic variability in survival after PWN inoculation, indicating that breeding...

The pinewood nematode (PWN) Bursaphelenchus xylophilus is the causal agent of the pine wilt disease (PWD) and represents one of the major threats to conifer forests. The detection of the PWN in Portugal, associated with Pinus pinaster, increased the concern of its spread to European forests. Despite its susceptibility to PWD, genetic variability fo...

Pine wilt disease (PWD), caused by the plant–parasitic nematode Bursaphelenchus xylophilus, has become a severe environmental problem in the Iberian Peninsula with devastating effects in Pinus pinaster forests. Despite the high levels of this species' susceptibility, previous studies reported heritable resistance in P. pinaster trees. Understanding...

The pinewood nematode (PWN) is the causal agent of pine wilt disease, a pathology that affects conifer forests, mainly Pinus spp. PWN infection can induce the expression of phytohormone-related genes; however, changes at the early phytohormone level have not yet been explored. Phytohormones are low-abundance metabolites, and thus, difficult to quan...

Background and Aims The development of an efficient clonal selection process requires the study of genotype‐by‐environment (G × E) interaction. This work aims to evaluate the variability of the G × E interaction among genotypes and to identify the less sensitive ones. Methods and Results The approach involves the fitting of mixed models to yield d...

In 1933, Joaquim Vieira Natividade established an experimental field with oaks and related genera at Mata Nacional do Vimeiro, Portugal. This experimental area includes the only Portuguese plots with the hybrid Quercus x hispanica hybrid ‘Lucombeana’ (Quercus cerris x suber) with seeds obtained from the Cambridge Botanic Gardens.In 1962-1965, their...

Key message This paper presents a greenhouse study for assessing the genetic variation in maritime pine (Pinus pinaster Aiton) in response to pinewood nematode (Bursaphelenchus xylophilus (Steiner et Buhren) Nickle), which is a causal agent of pine wilt disease. Fifteen out of 96 half-sib families were selected as less susceptible. This experiment...

Cork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork...

Cork oak is the main cork-producing species worldwide, and plays a significant economic, ecological and social role in the Mediterranean countries, in particular in Portugal and Spain. The ability to produce cork is limited to a few species, hence it must involve specific regulation mechanisms that are unique to these species. However, to date, the...

Atendendo ao lugar de destaque que o sobreiro detém em Portugal e à importância económica da cortiça, o INIAV tem coordenado vários projetos de investigação, para aprofundar o conhecimento sobre a genética desta espécie, como contributo para a avaliação precoce da qualidade do tecido suberoso e seleção juvenil e atempada das árvores boas produtoras...

Background and AimsThe evaluation of genotype-by-environment interaction and the genotypic correlations between important economic traits are two relevant issues of the methodology of grapevine selection that remain insufficiently explored. The aim of this study is to provide methodological tools to: (i) assess the genotype-by-environment interacti...

This study aimed to model the cone production weight of stone pine (Pinus pinea L.) trees using two approaches: classic linear mixed modelling and Bayesian mixed modelling. The field data were collected in the Setúbal Peninsula, the main production area in Portugal, where 51 plots for monitoring cone production per tree during three production peri...

Background Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long li...

Pinus pinea L. (Mediterranean stone pine) is an important forest species not only for its economically relevant kernel production, but also for environmental protection. The detection of genetic variability is an essential issue for Mediterranean forest species for conservation and improvement programs. Based on data collected for several years at...

The pine nut, the edible kernel of the Mediterranean stone pine, Pinus pinea, is one of the world's most expensive nuts. Although well known and planted since antiquity, pine nuts are still collected mainly from natural forests in the Mediterranean countries, and only recently has the crop taken the first steps to domestication as an attractive alt...

The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a serious quarantine pest first detected in Portugal and Europe in 1999. It is the causal agent of pine wilt disease (PWD). A resistance breeding programme has been initiated to contribute to control the evolution of the disease. Five hundred and four adult maritime pine, Pinus pinaster, t...

This work addresses to the genetic analysis and selection in populations where the whole genotypic value is transmitted through generations, using grapevine yield data as a case study. Several models were applied to different types of data sets. The individual and mean year yield and the balanced and unbalanced data resulting from various experimen...

The Pinewood nematode Bursaphelenchus xylophilus (PWN) was found to be responsible for maritime pine (Pinus pinaster) death for the first time in Europe in 1999, in a Portuguese forest south of Lisbon. It is the causal agent of the Pine Wilt Disease (PWD) and a quarantine organism in the European Union with approximately €80 million spent from 1999...

• Multivariate statistical analysis was used to define different developmental stages for stone pine (Pinus pinea L.) considering tree size and cone production, without site-specific information. • This was achieved in two steps. First, trees from permanent plots were classified using cluster analysis in five different stages. Second, discriminant...

The aim of this investigation was to evaluate the possibilities of vegetative propagation techniques of adult cork oak trees so as to develop an approach applicable to a genetic improvement breeding program. Bud grafting and cuttings were used to multiply and mass propagate selected cork oak trees. Forty-six cork oak trees were reproduced from clon...

1 UTL. Instituto Superior Agronomia. Centro de Estudos Florestais. Tapada da Ajuda 1349-017 LISBOA 2 EnerForest. Pólo Industrial da Portucel. Apartado 55, Mitrena, 2961-861 SETÚBAL 3 INRB. L-INIA. Unidade de Recursos Genéticos, Ecofisiologia e Melhoramento de Plantas. Quinta do Marquês, Av. da República, 2780-159 OEIRAS Resumo. O projecto Gestão Mu...