Irmgard Derakhshifar

Irmgard Derakhshifar
Österreichische Agentur für Gesundheit und Ernährungssicherheit | AGES · Department of Apiculture and Bee Protection

About

46
Publications
3,307
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712
Citations
Citations since 2017
3 Research Items
286 Citations
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20172018201920202021202220230204060
20172018201920202021202220230204060

Publications

Publications (46)
Conference Paper
Full-text available
Bee viruses pose a considerable risk for the health of honeybee colonies all over the world. In this project we aim to collect representative data for eight bee viruses in Austrian honeybee colonies to identify the most problematic virus species. Additionally, we want to raise the awareness of beekeepers for virus infections and to encourage them t...
Article
Full-text available
Austrian beekeepers frequently suffered severe colony losses during the last decade similar to trends all over Europe. This first surveillance study aimed to describe the health status of Austrian bee colonies and to analyze the reasons for losses for both the summer and winter season in Austria. In this study 189 apiaries all over Austria were sel...
Article
Full-text available
To evaluate the performance of the molecular methods used by national reference laboratories (NRLs) for the identification of Nosema species in bee samples, an inter-laboratory comparison (ILC) was organised in 2015. A total of 20 EU NRLs and 1 non-European NRL participated in this ILC. The specificity of the methods was tested on various Nosema sp...
Article
Full-text available
Genetic diversity of 214 Paenibacillus larvae strains from Austria was studied. Genotyping of isolates was performed by polymerase chain reaction (PCR) with primers corresponding to enterobacterial repetitive intergenic consensus (ERIC), BOX repetitive and extragenic palindromic (REP) elements (collectively known as rep-PCR) using ERIC primers, BOX...
Article
Full-text available
Honeybees originating from 10 different countries (Austria, Poland, Germany, Hungary, Slovenia, Nepal, Sri Lanka, the United Arab Emirates, Canada, and New Zealand) located on four continents were analyzed for the presence of deformed wing virus (DWV) nucleic acid by reverse transcription-PCR. Two target regions within the DWV genome were selected...
Article
Full-text available
The occurrence, prevalence, and distribution patterns of acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Kashmir bee virus (KBV), and sacbrood virus (SBV) were investigated in 90 Austrian honeybee colonies suffering from symptoms of depopulation, sudden collapse, paraly...
Article
Full-text available
PCR assays were developed for the direct detection of Paenibacillus larvae in honey samples and compared with isolation and biochemical characterization procedures. Different primer pairs, designed from the 16S rRNA and the metalloproteinase precursor gene regions, and different DNA extraction methods were tested and compared. The sensitivity of th...
Article
Full-text available
Sacbrood virus (SBV) infects larvae of the honeybee (Apis mellifera), resulting in failure to pupate and death. Until now, identification of viruses in honeybee infections has been based on traditional methods such as electron microscopy, immunodiffusion, and enzyme-linked immunosorbent assay. Culture cannot be used because no honeybee cell lines a...

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