Irina Yu Yanina

• PhD
• Professor (Associate) at Saratov National Research State University

Objectives The paper focuses on the development of technology of adipose tissue optical clearing using different complex hyperosmotic optical clearing agents and tissue permeability enhancers. Methods To quantify optical clearing efficiency, reduced scattering coefficient was estimated from the ex vivo spatially resolved backreflectance measuremen...

XXVIII Международной школы для студентов и молодых ученых по оптике, лазерной физике и биофотонике, который состоится 26 сентября в 14.00 в Большой физической аудитории III корпуса СГУ имени Н.Г. Чернышевского «БИОФОТОНИКА-НОВАЯ НАУКА НА СЛУЖБЕ ЧЕЛОВЕЧЕСТВА» Лектор: Член-корреспондент РАН, заслуженный деятель науки РФ, профессор РФ 2022 г., доктор...

The main goal of SFM'24 is to present and discuss the latest advances in the field of biophysics of optical and laser technologies in biology and medicine, fine mechanics and control of optical and physiological properties of tissues and cells, coherent optics of random and ordered media, materials and environmental sciences, nonlinear dynamics of...

(1) Background: Upconversion nanoparticles (UCNPs) are a promising tool for biological tissue structure visualization and photodynamic therapy (PDT). The luminescence of such NPs is excited in the spectrum’s near-infrared (NIR) region, while the NPs luminesce in the visible region. Conjugating such NPs with photodynamic dyes that absorb their lumin...

(1) Background: The use of electronic cigarettes has become widespread in recent years. The use of e-cigarettes leads to milder pathological conditions compared to traditional cigarette smoking. Nevertheless, e-liquid vaping can cause morphological changes in lung tissue, which affects and impairs gas exchange. This work studied the changes in morp...

Laser molecular imaging enables the acquirement of information related to tissue structure and 3D chemical content. The combination of similar data enables a more accurate estimation of tissue state and, through it, more precise medical diagnostics and treatment monitoring. However, not all measured parameters are equally valuable. This obstacle re...

Adipose tissue (AT) optical properties for physiological temperatures and in vivo conditions are still insufficiently studied. The AT is composed mainly of packed cells close to spherical shape. It is a possible reason that AT demonstrates a very complicated spatial structure of reflected or transmitted light. It was shown with a cellular tissue ph...

Background and Objectives: Knowledge of the optical and thermal human tissues properties is essential for optimizing laser therapy and optical diagnostics. Most studies are carried out at room temperature of the sample. At the same time, it is known that the optical properties of biological tissues depend on temperature even in the physiological te...

Deadline for manuscript submissions: 28 February 2023

In this paper, the measurement and modeling of optical properties in the terahertz (THz) range of adipose tissue and its components with temperature changes were performed. Spectral measurements were made in the frequency range 0.25–1 THz. The structural models of main triglycerides of fatty acids are constructed using the B3LYP/6-31G(d) method and...

Optical clearing of the lung tissue aims to make it more transparent to light by minimizing light scattering, thus allowing reconstruction of the three-dimensional structure of the tissue with a much better resolution. This is of great importance for monitoring of viral infection impact on the alveolar structure of the tissue and oxygen transport....

Optical clearing of adipose tissue has not been studied enough, although it can be promising in medical applications, including surgery and cosmetology, for example, to visualize blood vessels or increase the permeability of tissues to laser beams. The main objective of this work is to develop technology for optical clearing of abdominal adipose ti...

This chapter discusses the optical clearing (OC) of adipose tissue ex vivo and in vivo, which has not yet been described but can be useful in optical diagnostics, therapy, and surgery when the site where it is necessary to carry out light exposure is covered by adipose tissue, which is typical in the case of many important internal organs. The chap...

Optical techniques and laser diagnostics are becoming integral elements in engineering and medical instrumentation. Written by leading experts from academic groups and laboratories, this Special Issue provides an international perspective on both existing applications and cutting-edge research. With a focus on advanced biophotonics applications, th...

The main goal of SFM’21 is to present and discuss recent developments and applications of optical and laser technologies in biology and medicine, precise mechanics and control of tissues and cells, coherent optics of random and ordered media, material and environmental sciences, nonlinear dynamics of laser systems, laser physics, spectroscopy and m...

Terahertz (THz) spectroscopy of skin pathologies associated with water migration and content were analyzed using the method of immersion optical clearing. It shows different behavior THz spectra for healthy and diabetic skin after application of an optical clearing agent. It method can be used to identify skin pathologies. There is a migration of w...

Background and Objectives: The object of the study was the toxicity of upconversion nanoparticles. The aim is to overview the literature on the toxicity of various types of upconversion nanoparticles and to search for their maximum permissible concentration when administered to laboratory animals in vivo. Materials and Methods: The approach used ha...

The main goal of the Symposium is to present and discuss recent developments and applications of optical and laser technologies in medicine and biology, precise mechanics and control of tissues and cells, coherent optics of random and ordered media, material and environmental sciences, nonlinear dynamics of laser systems, laser physics, spectroscop...

The effect of glycerol solutions of different concentrations and exposure times on the optical clearing efficiency in intact pig skin has been studied in the analysis of Raman spectra recorded at different depths. It has been found that a solution of 80% glycerol and 20% dimethylsulfoxide penetrated through the stratum corneum during 45 min. The in...

The differences in the luminescence intensities of up-conversion β-NaYF 4 : Er ³⁺ , Yb ³⁺ particles synthesised by the hydrothermal method under various synthesis conditions are studied. The results of the study lead to the conclusion that in order to achieve the maximum luminescence intensity in such particles, it is necessary to use ammonium fluo...

The main objective of this work is to quantify the impact of photodynamic/photothermal treatment by using visible LED and NIR laser irradiation through the skin of subcutaneous fat in vivo followed up by tissue sampling and histology. The optical method may provide reduction of regional or site‐specific accumulations of abdominal or subcutaneous ad...

Conferences and Workshops of VII Symposium on Optics & Biophotonics: Ø Optical Technologies in Biophysics & Medicine XXI Ø Laser Physics and Photonics XXI Ø Spectroscopy and Molecular Modeling XX Ø Electromagnetics of Microwaves, Submillimeter&Optical Waves XIX Ø Nanobiophotonics XV Ø Internet Biophotonics XII Ø Microscopic and Low-Coherence Method...

Photoluminescence spectra of the up-conversion nanoparticles NaYF4 : Y³⁺, Er³⁺ arranged under a layer of an adipose tissue have been recorded in a wide temperature range. It is shown that if nanoparticle temperature is determined from the photoluminescence spectra, an error arises related to spectrum distortion due to light scattering in a sample....

The skin is the biggest organ of human organism playing an important physiological function of barrier between the organism and environment. The main skin functions are regulation of the water loss, insulation and thermo-regulation as well as protection of the internal organs from external mechanical impact and penetration of pathogens. In this way...

Nowadays, dynamically developing optical (photonic) technologies play an ever-increasing role in medicine. Their adequate and effective implementation in diagnostics, surgery, and therapy needs reliable data on optical properties of human tissues, including skin. This paper presents an overview of recent results on the measurements and control of t...

Enzymatic destruction of adipose tissue has been achieved by encapsulation of lipase into the polymeric microcapsules. Adipose tissue destruction was delayed while lipase is encapsulated comparing to the direct lipase action as demonstrated by optical microscopy and optical coherence tomographyin in vitro studies. Raman spectroscopy confirms that t...

This study presents refractive index measurements of human and porcine adipose tissues and lipid droplet content in the visible and near-infrared. The coefficients of the Sellmeier formula were calculated for approximation of tissue dispersion. For the first time, to the best of our knowledge, the phase transition temperatures and temperature incre...

Dear colleagues, The Organizing Committee of SFM’18 inviting you to attend the 22nd Annual Saratov Fall Meeting 2018. For participation, it is necessary to fill up the attached registration forms before August 1, 2018 and send it via e-mail to Polina Timoshina (Symposium) timoshina2906@mail.ru . At this stage, we need a title and a short abstra...

Dear colleagues, The Organizing Committee of SFM’18 inviting you to attend the 22nd Annual Saratov Fall Meeting 2018. For participation, it is necessary to fill up the attached registration forms before August 1, 2018 and send it via e-mail to Irina Yanina (School) irina-yanina@yandex.ru. At this stage, we need a title and a short abstract of y...

The luminescence spectra of upconversion nanoparticles (UCNPs) and ZnCdS nanoparticles (ZnCdSNPs) were measured and analyzed in a wide temperature range: from room to human body and further to a hyperthermic temperature resulting in tissue morphology change. The results show that the luminescence signal of UCNPs and ZnCdSNPs placed within the tissu...

The luminescence spectra of ZnCdS nanoparticles (ZnCdS NPs) were measured and analyzed in a wide temperature range: from room to human body and further to a hyperthermic temperature resulting in tissue morphology change. The results show that the luminescence signal of ZnCdS NPs placed within the tissues is reasonably good sensitive to temperature...

Delivery and spatial localization of upconversion luminescent microparticles [Y2O3:Yb, Er] (mean size ∼1.6 μm) and quantum dots (QDs) (CuInS2/ZnS nanoparticles coated with polyethylene glycol-based amphiphilic polymer, mean size ∼20 nm) inside rat skin was studied in vivo using a multimodal optical imaging approach. The particles were embedded into...

Observation of temperature-mediated phase transitions between lipid components of the adipose tissues has been performed by combined use of the Abbe refractometry and optical coherence tomography. The phase transitions of the lipid components were clearly observed in the range of temperatures from 24°C to 60°C, and assessed by quantitatively monito...

The goal of this study is to quantify the impact of the in vivo photochemical treatment of rats with obesity using indocyanine green (ICG) dissolved in saline or dispersed in an encapsulated form at NIR laser irradiation, which was monitored by tissue sampling and histochemistry. The subcutaneous injection of the ICG solution or ICG encapsulated in...

In the frameworks of developing ecophotonics, we consider the possibility of applying luminescence spectroscopy for monitoring conditions of aquatic organisms, aimed at the study and prognosis of the effect of human activity and climate changes on the environment. The method of luminescence spectroscopy in combination with anti-Stokes luminophores...

We have developed a method for delivery of biocompatible CaCO3 microcontainers (4.0 ± 0.8 µm) containing Fe3O4 nanoparticles (14 ± 5 nm) into skin in vivo using fractional laser microablation (FLMA) provided by a pulsed Er:YAG laser system. Six laboratory rats have been used for the microcontainer delivery and weekly monitoring implemented using an...

The goal of this work is to study kinetics of optical properties of abdominal fat tissue in the course of temperature change and to design the model of this process. The offbeat optical properties of adipose tissues in norm and at thermal action were recently found (Irina Y. Yanina, Valery V. Tuchin, Valery A. Doubrovsky, Alexander V. Bykov, “The off...

Delivery of upconversion microparticles [Y2O3:Yb, Er] and quantum dots (CuInS2/ZnS coated with PEG-based amphiphilic polymer) into rat skin using the fractional laser microablation has been studied in vivo. Luminescence spectroscopy, optical coherence tomography, confocal microscopy, and histochemical analysis were used for visualization of nanopar...

The goal of this work is to quantify impact of in vivo photochemical treatment using indocyanine green (ICG) or encapsulated ICG and NIR laser irradiation through skin of rat with obesity by the follow up tissue sampling and histochemistry. After 1 hour elapsed since 1-min light exposure samples of rat skin with subcutaneous tissue of thickness of...

Temperature dependence of the fluorescence spectrum of ZnCdS nanoparticles introduced into 200-500 μm thick fat tissue slices in vitro was studied. The heating of the samples from the room to physiological temperature results in stronger (in depth) and faster tissue morphology change. This can help to detect location of nanoparticles during fat cel...

A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical propertie...

A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical propertie...

The effect of IR-laser irradiation on adipose tissue sensitized by indocyanine green is studied in vitro. Experiments and statistical analysis are used to show that wavelength-selective irradiation leads to an increase in the homogeneity of optical images of adipose cells with time. The transmission coefficient that is averaged over the image area...

Delivery of upconversion microparticles [Y2O3:Yb, Er] and quantum dots (CuInS2/ZnS coated with PEG-based amphiphilic polymer) into rat skin using the fractional laser microablation has been studied in vivo. Luminescence spectroscopy, optical coherence tomography, confocal microscopy, and histochemical analysis were used for visualization of nanopar...

Monitoring of temperature-mediated adipose tissue phase transitions were studied in vitro using an Abbe refractometer. The 1-2-mm thick porcine fat tissues slices were used in the experiments. The observed change in the tissue was associated with several phase transitions of lipid components of the adipose tissue. It was found that overall heating...

Morphological changes of the adipose tissue at phototreatment are studied in vitro using optical coherence tomography. The 200 to 600 μm fat tissue slices are used in the experiments. The observed change in the tissue structure was associated with fat cell lipolysis and destruction caused by the photodynamic effect. It is found that overall heating...

Structures that can be interpreted as cytoplasm droplets leaking through the membrane are experimentally detected on the membranes of adipocytes using optical digital microscopy. The effect of an aqueous alcohol solution of brilliant green on the amount and sizes of structures is studied. It is demonstrated that the optical irradiation of the adipo...

The dependence of the spatial resolution and field of view of an optical microscope equipped with a CCD camera on the objective magnification has been experimentally investigated. Measurement of these characteristics has shown that a spatial resolution of 20–25 px/μm at a field of view of about 110 μm is quite realistic; this resolution is acceptab...

Destructive fat tissue engineering could be realized using the optical method, which provides reduction of regional or site-specific accumulations of subcutaneous adipose tissue on the cell level. We hypothesize that light irradiation due to photodynamic and selective photothermal effects may lead to fat cell lypolytic activity (the enhancement of...

Temporal changes in structure and refractive-index distribution of adipose tissue at photodynamic/photothermal treatment were studied with OCT in vitro. Ethanol–water solutions of indocyanine green (ICG) and brilliant green (BG) were used for fat tissue staining. CW laser diode (808 nm) and LED light source (442 and 597 nm) were used for irradiatio...

The changes in optical transmittance of human adipose cell layers sensitized by indocyanine green (ICG) as a result of photodynamic action were found and studied. It was revealed experimentally that due to the selective action of laser radiation on fat tissue sensitized by ICG the spatial distribution of its optical transmittance becomes more homog...

Histological slices of skin samples with the subcutaneous adipose tissue after photothermal/photodynamic treatment are analyzed. In the case of subcutaneous indocyanine green injection and 808-nm diode laser exposure of the rat skin site in vivo, the greatest changes in tissue condition were observed. Processes were characterized by dystrophy, necr...

The "specific structures" on the fat cells' membranes in vitro as a result of photodynamic treatment was registered. These structures were identified as cytoplasm/oil microdrops flowed out through the pores in the membranes. The impact of Brilliant Green dissolved in water-ethanol solutions and irradiation by a LED lamp on the quantity and size of...

The influence of bacterial lectin on photochemically induced fat cell lipolysis was studied. Resulting capsules were tested for ICG absorption by optical spectra measurements. To separate released and encapsulated ICG supernatant was removed and capsules were redispered in pure deionized water. Supernatant and capsule suspension spectra were measur...

Changes in the light transmission coefficient of human adipose cells as a result of photodynamic action have been detected and investigated. Based on the statistical processing of digital micrographs of thin tissue slices, the kinetics of processes occurring in the adipose tissue has been quantitatively estimated. It has been suggested that the eff...

Time variation of the adipose tissue refractive index under photodynamic treatment was studied using OCT. Fat tissue slices 200-500 μm thick were used in in vitro experiments. To stain the fat tissue we used water-ethanol solutions of indocyanine green (ICG) and brilliant green (BG) with the concentration 1 mg/ml and 6 mg/ml, respectively. The CW l...

The effect of a change in the optical properties of human adipose tissue cells in vitro after photodynamic action was studied experimentally. The study of kinetics of this process was carried out based on the digital microscopy of thin layers of tissue. The statistical computer processing of the obtained microimages has allowed one to quantitativel...

Lipid peroxidation (LP) of blood serum of laboratory animals after in vivo photodynamic treatment was investigated. To determine changes in LP the standard colorimetric test OXYSTAT was used. The results indicate an increase in the intensity of free radical generation in tissues induced by photodynamic treatment.

Fat tissue engineering by near-infrared laser treatment is investigated in vitro. For the quantitative estimation of efficiency of treatment and consequential processes on the cellular level, a computational method for optical image analysis is proposed. The method is based on the fractal dimension data analysis of fat cell structure. The conducted...

Histological slices of skin samples with the subcutaneous adipose tissue after laser irradiation at different doses are analyzed. These data may be used at carrying out of the analysis of histological slices of skin samples with the subcutaneous adipose tissue after photodynamic therapy. The obtained data are important for safe layer-by-layer dosim...

Photodynamic therapy with temperature control is a new approach for treatment of obesity and cellulite. Cell death can occur under the action of various physical, chemical and biological factors. Depending on the inductor, this is apoptosis or necrosis. These two forms of cell death differ on the biochemical and morphological levels. Biochemical ch...

Changes in optical properties of human adipose tissue cells after photodynamic exposure in vitro were found and investigated. Analysis of the kinetics of the process was realized by means of photomicrography of the object investigated. The statistical computer processing of digital photos obtained gave us an opportunity to estimate quantitatively t...

The effect of optical properties changes of adipose tissue cells in vitro as a result of photoaction was found and investigated. The experimental study of photo-induced post action upon the cells of fat tissue by means of digital microscopy was fulfilled. The computer processing of digital photos obtained gave an opportunity to estimate quantitativ...

We study experimentally the optical characteristics of adipose tissue in vitro in its dye sensitization—by indocyanin green and brilliant green. We conducted experiments on a PerkinElmer Lambda 950 spectrophotometer in the spectral range of 250–900 nm. From an analysis of the measured absorption spectra, we determine the shifts in the maximum of th...

Cellulite is considered as a disease of the subcutaneous fat layer that appears mostly in women and consists of changes in fat cell accumulation together with disturbed lymphatic drainage, affecting the external appearance of the skin. The photodynamic and selective photothermal treatments may provide reduction the volume of regional or sitespecifi...

Destructive fat tissue engineering using photodynamic and photothermal action is investigated in vitro. Samples of fat tissue were processed by a photosensitizer indocyanine green (ICG) and irradiated by a diode laser (805 nm). Computational methods of photo analysis are proposed for the quantitative estimation of the efficiency of actions on the c...