Irina Alexandrova

• Burdenko Neurosurgery Institute

The search for new potential biomarkers for the diagnostics of post-neurosurgical bacterial meningitis is required because of the difficulties in its early verification using results of the routine laboratory and biochemical analyses of the cerebrospinal fluid (CSF). The goal of the study was to determine the contents of the aromatic metabolites an...

Klebsiella pneumoniae is an increasingly important hospital pathogen. Classical K. pneumoniae (cKp) and hypervirulent K. pneumoniae (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of K. pneumoniae resulted in the generation of hybrid hvKP-MDR strains. K. pneumoniae distinct isolates (n = 70) belonged to 20 sequenc...

The purpose of this study was the identification of genetic lineages and antimicrobial resistance (AMR) and virulence genes in Klebsiella pneumoniae isolates associated with severe infections in the neuro-ICU. Susceptibility to antimicrobials was determined using the Vitek-2 instrument. AMR and virulence genes, sequence types (STs), and capsular ty...

Multidrug resistance (MDR) and hypervirulence (hv) have been long considered distinct evolutionary traits for Klebsiella pneumoniae (Kp), a versatile human pathogen. The recent emergence of Kp strains combining these traits poses a serious global threat. In this article, we describe the phenotypic and genomic characteristics of an MDR hvKp isolate,...

Between February-2013 and November-2015 a nosocomial outbreak due to hypervirulent carbapenemase-producing Klebsiella pneumoniae (Kpn) occurred in the neurosurgical ICU in Moscow, Russia. Kpn isolate MAR14456 representing this outbreak was isolated in February-2014 from postoperative wound in a patient who later developed multiple abscesses, fatal...

Background The impact of infection prevention and control (IPC) programs in limited resource countries such as Russia are largely unknown due to a lack of reliable data. The aim of this study is to evaluate the effect of an IPC program with respect to healthcare associated infection (HAI) prevention and to define the incidence of HAIs in a Russian...

The antibacterial resistance and virulence genotypes and phenotypes of 148 non-duplicate Klebsiella pneumoniae strains collected from 112 patients in Moscow hospitals in 2012–2016 including isolates from the respiratory system (57%), urine (30%), wounds (5%), cerebrospinal fluid (4%), blood (3%), and rectal swab (1%) were determined. The majority (...

The juvenile nasal angiofibroma (JNA) surgeries are very often complicated by massive intraoperative blood loss. In order to compensate it the intraoperative blood reinfusion of autologous erythrocytes from operative field by cell-saver is very often used. But the high probability of chronic inflammation of blocked sinuses in patients with JNA rais...

We report genome sequences of two NDM-1 metallo-β-lactamase-producing multidrug-resistant Klebsiella pneumoniae isolates of sequence type 147 (ST147) from one hospital. The genomes are highly similar and differ in prophage located in the chromosome of K. pneumoniae KPB-1470/16 and in the additional plasmid-carrying blaOXA-48 gene in K. pneumoniae K...

Aim: to determine the incidence rate and risk factors for drainage-associated meningitis in neurocritical care patients. Material and methods: The prospective study included 539 patients who spent more than 48 h at the Department of Neurocritical Care and underwent external ventricular drainage. The incidence rate and risk factors for drainage-a...

Aim: To determine the incidence rate and risk factors of nosocomial meningitis in patients with complicated postoperative period. Material and methods: We report the results of prospective observation of the course of bacterial meningitis in patients with complicated postoperative period at neurosurgical intensive care unit in 2010-2014. Result...

Background The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a great problem of healthcare worldwide. Study of the spread for blaOXA-48-like genes coding epidemically significant carbapenemases among hospital pathogens is important for the regional and global epidemiology of antimicrobial resistance. Methods Antibacterial resistant...

Data collected in electronic patient records (EPR) databases may be used for different goals besides their direct purpose - information support for medical staff in their everyday activities. One of the most promising spheres is a monitoring of various processes induced in medical facility. In our studies EPR serves as a basis for healthcare associ...

Clin Microbiol Infect 2012; 18: E214–E217 An Escherichia coli isolate co-producing VIM-4 metallo-β-lactamase and CTX-M-15 extended spectrum β-lactamase was recovered from the urine of a patient with head trauma in Moscow, Russia. The blaVIM-4 and blaCTX-M-15 genes were carried, respectively, by transmissible plasmids of IncW and IncI1 groups. The n...

This work is a report on the development of a method of hybridization analysis on DNA microarrays for the simultaneous identification and typing of carbapenemase-encoding genes. These enzymes are produced by the microorganisms that are responsible for causing infectious diseases. The method involves several steps, including DNA extraction from clin...

Nosocomial infections and antimicrobial resistance (AR) are highly important problems that impact the morbidity and mortality of hospitalized patients as well as their cost of care. The goal of this paper is to demonstrate our analysis of AR by applying a number of various data mining (DM) techniques to real hospital data. The data for the analysis...