Irene Villar

CRAG Centre for Research in Agricultural Genomics | CRAG · Department of Plant Genetics

Resistance to Cucumber mosaic virus (CMV) in melon (Cucumis melo L.) has been described in several exotic accessions and is controlled by a recessive resistance gene, cmv1, that encodes a Vacuolar protein sorting 41 (CmVPS41). cmv1 prevents systemic infection by restricting the virus to the bundle sheath cells, preventing viral phloem entry. CmVPS4...

Resistance to Cucumber mosaic virus (CMV) in melon has been described in several exotic accessions. It is controlled by a recessive resistance gene, cmv1, which encodes a Vacuolar Protein Sorting 41 (CmVPS41). Cmv1 prevents systemic infection by restricting the virus to the bundle sheath cells, preventing viral phloem entry. CmVPS41 from different...

Legumes express two major types of hemoglobins: symbiotic (leghemoglobins) and non-symbiotic (phytoglobins). The latter are categorized into three classes according to phylogeny and biochemistry. Using knock-out mutants, we show that all three phytoglobin classes are required for optimal vegetative and reproductive development of Lotus japonicus. F...

The recently identified nonsymbiotic hemoglobin gene MtGlb1-2 of the legume Medicago truncatula possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing one and two hemes, respectively. Unexpectedly,...

Among legumes (Fabaceae) capable of nitrogen-fixing nodulation, several Aeschynomene spp. use a unique symbiotic process that is independent of Nod factors and infection threads. They are also distinctive in developing root and stem nodules with photosynthetic bra-dyrhizobia. Despite the significance of these symbiotic features, their understanding...

[This corrects the article DOI: 10.3389/fpls.2020.600336.].

Among legumes (Fabaceae) capable of nitrogen-fixing nodulation, several Aeschynomene spp. use a unique symbiotic process that is independent of Nod factors and infection threads. They are also distinctive in developing root and stem nodules with photosynthetic bradyrhizobia. Despite the significance of these symbiotic features, their understanding...

In plants, symbiotic hemoglobins act as carriers and buffers of O2 in nodules, whereas nonsymbiotic hemoglobins or phytoglobins (Glbs) are ubiquitous in tissues and may perform multiple, but still poorly defined, functions related to O2 and/or nitric oxide (NO). Here, we have identified a Glb gene of the model legume Medicago truncatula with unique...

Legume nodules have two types of hemoglobins: symbiotic or leghemoglobins (Lbs) and nonsymbiotic or phytoglobins (Glbs). The latter are categorized into three phylogenetic classes differing in heme coordination and O2 affinity. This review is focused on the roles of Lbs and Glbs in the symbiosis of rhizobia with crop legumes and the model legumes f...

Symbiotic hemoglobins provide O2 to N2‐fixing bacteria within legume nodules, but the functions of nonsymbiotic hemoglobins or phytoglobins (Glbs) are much less defined. Immunolabeling combined with confocal microscopy of the Glbs tagged at the C‐terminus with green fluorescent protein was used to determine their subcellular localizations in Arabid...

An efficient plant regeneration protocol has been established for two commercial Populus hybrid clones, MC (Populus × euramericana) and UNAL (Populus × interamericana). The culture of internode segments on Murashige and Skoog (MS) medium with 0.5 μM α-naphthalene acetic acid (NAA) and 4 μM N⁶-benzyladenine for 7 weeks (2 weeks in absence of activat...

El cáncer es el término que se utiliza para englobar un conjunto de enfermedades que se caracterizan por el crecimiento descontrolado de célular alteradas molicularmente por mutaciones o modificaciones epigenéticas. En la presente revisión describimos algunas terapias dirigidas que se están utilizando actualmente en clínica.