Irene Meester- PhD Immunology
- Professor at Universidad de Monterrey
Irene Meester
- PhD Immunology
- Professor at Universidad de Monterrey
About
45
Publications
9,736
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
393
Citations
Introduction
Currrent research interests:
1. Neuroimmunology (Fibromyalgia, Alzheimer disease etiology)
2. Sex differences in immunity (XY chromosomes and epigenetics)
3. Whole body donation to science
Current institution
Additional affiliations
August 2013 - present
Position
- professor/investigator
Description
- Teaching: embryology, histology, immunology Investigation: 1) Immune Response in (latent) TB, 2) Borreliosis 3) Late-onset Alzheimer´s Disease due to infection? 4) Fibromyalgia & chronic fatigue syndrome due to infection?
August 2013 - December 2013
Position
- teacher/instructor
Description
- Immunology & Microbiology, Cell Biology & Genetics
August 2013 - June 2014
Publications
Publications (45)
Electrospun membranes (EMs) have a wide range of applications, including use as local delivery systems. In this study, we manufactured a polyurethane Tecoflex™ EM loaded with bismuth-based lipophilic nanoparticles (Tecoflex™ EMs-BisBAL NPs). The physicochemical and mechanical characteristics, along with the antitumor and bactericidal effects, were...
Bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) are antineoplastic and antimicrobial in vitro. As a next pre-clinical step, a clinically viable dosage form for vaginal application was developed. Compendial pharmacopeial tests (mass uniformity, disintegration, and compressive mechanics) and inductively coupled plasma...
Objective
To evaluate the antitumor and antimicrobial properties of an alginate-based membrane (ABM) loaded with bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) on clinically isolated bacteria and a pancreatic cancer cell line.
Material and methods
The BisBAL NP-CPC ABM was characterized using optical and scanning...
![Post-mortem donation survey results. [#] item number within the PD...](publication/375914212/figure/fig2/AS:11431281206880051@1700967865750/Post-mortem-donation-survey-results-item-number-within-the-PD-questionnaire_Q320.jpg)
Background
Voluntary post-mortem donation to science (PDS) is the most appropriate source for body dissection in medical education and training, and highly useful for biomedical research. In Mexico, unclaimed bodies are no longer a legal source, but PDS is legally possible, although scarcely facilitated, and mostly ignored by the general population...
Background
. Voluntary post-mortem donation to science (PDS) is the most appropriate source for body dissection to medical education and training, and highly useful for biomedical research. In Mexico, unclaimed bodies are no longer a legal source, but PDS is legally possible, though scarcely facilitated, and mostly ignored by the general population...
XISTexpression occurs in human somatic cells possessing more than one X chromosome to balance gene expression and in male germline cells to ensure X chromosome inactivation. A study designed to investigate the sex-biased expression of X-linked immune genes convincingly and repeatedly found low-level XISTexpression in male leukocytes.
RNA was extrac...
Objective:
To determine the combined antitumor effect of bismuth lipophilic nanoparticles (BisBAL NP) and cetylpyridinium chloride (CPC) on human lung tumor cells.
Material and methods:
The human lung tumor cells A549 were exposed to 1-100 µM BisBAL NP or CPC, either separately or in a 1:1 combination. Cell viability was measured with the Presto...
Objective
Analyze the antitumor capacity of cetylpyridinium chloride (CPC) on human breast tumor cells, and the possible action mechanism.
Material and methods
The human breast tumor cells MCF-7 and no-tumor breast cells MCF-10A were exposed to CPC under various condition (concentration and duration). Cell viability was measured with MTT assay, th...
Aim
The objective of this study was to analyze the antitumor effect of BisBAL NP in a mouse melanoma model.
Material and Methods
The antitumor activity of BisBAL NP on murine B16-F10 melanoma cells was determined both in vitro (PrestoBlue cell viability assay and Live/Dead fluorescence) and in vivo, in a mouse model, with the following 15-day trea...
The objective of this study was to study the synergistic antitumor effect of lipophilic bismuth nanoparticles (BisBAL NPs) with the antibiotic solution Neo-Poly gramicidin on human cervical tumor cells. The effect of BisBAL NPs and Neo-Poly gramicidin solution on cervical cancer cell line (HeLa) was determined by the MTT cell viability assay and fl...
Atorvastatin (ATV) is a blood cholesterol-lowering drug used to prevent cardiovascular events, the leading cause of death worldwide. As pharmacokinetics, metabolism and response vary among individuals, we wanted to determine the most reliable metabolic ATV phenotypes and identify novel and preponderant genetic markers that affect ATV plasma levels....
Objective
As sex bias in immune function has been contributed partly to a preponderance of immune system-related genes (ISRG) on the X-chromosome, we performed a comparative study to verify this viewpoint.
Methods
The comparative study consisted of three complementary strategies:
Results
The X chromosome had less ISRG than the median of the autos...
The fibromyalgia syndrome (FMS) is characterized by chronic widespread pain, sleep disturbances, fatigue, and cognitive alterations. A limited efficacy of targeted treatment and a high FMS prevalence (2–5% of the adult population) sums up to high morbidity. Although, altered nociception has been explained with the central sensitization hypothesis,...
Background
Sex bias in immune function has been contributed in part to a preponderance of immune system-related genes (ISRG) on the X-chromosome. We verified whether ISRG are more abundant on the X chromosome as compared to autosomal chromosomes and reflected on the impact of our findings.
Methods
Consulting freely accessible databases, we perfor...
The objective of this study was to compare the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine (CHX) on human squamous cell carcinoma. BisBAL NPs were synthesized by colloidal method and characterized by energy dispersive X-ray spectroscopy in conjunction with scanning electron microscopy (EDS-SEM). The effect...
The objective of this study was to analyze the antitumor activity of a hydrogel loaded with lipophilic bismuth nanoparticles on human cervical, prostate, and colon cancer cell lines. The effect of lipophilic bismuth nanoparticles on the viability of cancer cell lines (HeLa, DU145, and HCT-116) and non-cancer lung fibroblasts (HLF; LL 47[MaDo]) was...
A ventricular aneurysm entails well-known risks for the patient such as heart failure, potentially lethal arrhythmias, and systemic embolic phenomena. The submitral or posterolateral ventricular aneurysm is a very rare variety, usually of congenital etiology, which may also have other causes, including ischemic heart disease. The present case is ab...
Purpose
The purpose of this study was to describe diabetes self-management behaviors, diabetes health care access, and health perception for Mexican adults and Hispanics residing in the Mexico-US border region.
Methods
This study used data from the Behavior Risk Factor Surveillance System (BRFSS) survey diabetes items (n = 26) to assess characteri...
Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUP...
Type 2 diabetes mellitus (T2DM) is one of the leading causes of death from worldwide non-communicable diseases. The prevalence of diabetes in the Mexico (MX)–United States border states exceeds the national rate in both countries. The economic burden of diabetes, due to decreased productivity, disability, and medical costs, is staggering and increa...
Objective:
The purpose of this study was to assess esophageal damage in patients with recessive dystrophic epidermolysis bullosa (RDEB) with or without dysphagia.
Subjects and methods:
Fourteen patients with either severe generalized or another generalized form of RDEB recruited through a research and support foundation were evaluated for obstru...
Abstract
Background
The genetic variation underlying atorvastatin (ATV) pharmacokinetics was evaluated in a Mexican population. Aims of this study were: 1) to reveal the frequency of 87 polymorphisms in 36 genes related to drug metabolism in healthy Mexican volunteers, 2) to evaluate the impact of these polymorphisms on ATV pharmacokinetics, 3) to...
Alzheimer's disease (AD) is characterized by a progressive decay of cognitive abilities, most remarkably (spatial) memory and learning. AD is diagnosed by clinical mental tests, often combined with the detection of neurobiological markers, mainly brain imaging studies and a decreased amyloid beta (Aß) level and/or increased total and hyper-phosphor...
Recessive dystrophic epidermolysis bullosa (R-DEB) is caused by mutations in the COL7A1 gene. The most common mutation reported in Mexican families is the c.2470insG mutation, normally detected by DNA sequencing. We report a faster and more economical high-throughput genotyping method to detect the c.2470insG mutation using specific TaqMan probes i...
Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vi...
Nocardia brasiliensis (Nb) is a facultative intracellular pathogen that may cause actinomycetoma when immune response is unable to control the pathogenic invasion. We used comparative real-time PCR to evaluate the expression level of molecules indicative of either classical or alternative activation of macrophages, as well as of cytokines involved...
Nocardia brasiliensis (Nb) is a facultative intracellular pathogen that may cause actinomycetoma when immune response is unable to control the pathogenic invasion. We used comparative real-time PCR to evaluate the expression level of molecules indicative of either classical or alternative activation of macrophages, as well as of cytokines involved...
A variety of materials are use to stimulate the alveolar bone regeneration, among them, some growth factors. Objectives: In this work we analyzed the osteoinductive capacity of BMP2 and TGF-beta, the growth of bone tissue and production of mineralization factors (SPARC and osteocalcin) was measured. Methods: A rat model of a calvarial defect was cr...
Foamy cells (FC) have been described in various infectious diseases, and can be usurped by intracellular pathogens for survival. In a mouse model of experimental actinomycetoma induced by Nocardia brasiliensis, FC have been observed at day 3 after inoculation, remaining constant during acute inflammation, and expanding in surface area during chroni...
TGF‐beta is involved in several cellular processes, including acting as a growth factor.
Objectives
The aim of this study was to investigate the effect of TGF‐beta on bone regeneration and osteocalcin production.
Methods
Calvarial osteotomy defects were created in adult male Sprague‐Dawley rats, they received TGF‐beta in an absorbable collagen sp...
There are several ways of analyze the ways of learning, one of the most accepted is the VARK questionnaire. In this work we investigated the preferred way of learning of students of the course of physiology of the faculty of odontology of the UANL of Monterrey, Mexico. The VARK questionnaire was apply to students of the course of physiology (n= 323...
Background:
Cytokines communicate between the cells of the immune system and its targets to maintain homeostasis after injury or pathogenic events. They are involved in almost any pathological situation imaginable.
Objective:
To verify the importance of cytokines as biomarkers in current preclinical (aetiopathogenic, development of new therapies...
Actinomycetoma, caused by the intracellular bacterium Nocardia brasiliensis, is characterized by an infiltration of several inflammatory cell populations. To explore aspects of the immune response in the pathogenesis of these bacteria we injected 10(6) CFU in footpads of BALB/c mice. After 1, 2, 3, 4, 7, 30 and 90 days immunohistochemistry was perf...
In the pond snail Lymnaea stagnalis, the growth regulating system consists of (1) about 200 neuroendocrine light green cells, located in four clusters in the cerebral ganglia, and (2) the paired canopy cells, located in the lateral lobes. These cells express genes encoding the molluscan insulin-related peptides (MIPs). Six MIP genes have previously...
A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP II cDNA. The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putati...
A cDNA clone encoding molluscan insulin-related peptide (MIP) II was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP cDNA (renamed MIP-I cDNA). The MIP-II cDNA encodes a preprohormone resembling the organization of preproinsu...
Questions
Questions (2)
The 11/18 tender point criterium was at the center of the ACR-1990 criteria for fibromyalgia. The ACR 2010/2011 and its 2016 revision is a dichotomous definition centered around chronic (> 3 months) widespread pain in 4/5 regions, and does not consider the 11/18 tender points any longer. But in practice, 1) is the tender point count still executed? 2) is it considered in establishing the diagnosis?
Thank you in advance for you reply.
In order to extract DNA from blood on FTA cards, Chelex-100 (BioRad) and FTA purification reagent (Whatman) seem to be the simplest, easiest, and, importantly, best-performing methods. I would appreciate a recommendation.
I found info on the principle of action of Chelex 100, but couldnot find any such information for the Whatman reagent. If anybody knows, please share your knowledge. Any responders, Thank you in advance.
