Ioannis StringlisAgricultural University of Athens · Laboratory of Phytopathology
Ioannis Stringlis
PhD in Plant-Microbe Interactions
About
37
Publications
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Introduction
During my bachelor and MSc internships, I studied the interaction between a beneficial Fusarium and soil borne pathogens of tomato and eggplant and the role of GPCRs in the biology and pathogenicity of soilborne pathogen Verticillium dahliae. During my PhD, I explored how roots perceive elicitors and beneficial rhizobacteria and the role of root exudation on root microbiome assembly. As a postdoc, I study the role of root defenses and the metabolomic changes during colonization. In parallel, I have keen interest on how plants can shape their microbiome via the exudation of secondary metabolites.
Additional affiliations
November 2017 - present
May 2012 - October 2017
February 2010 - April 2012
Education
September 2003 - December 2008
Publications
Publications (37)
Belowground, microbe-associated molecular patterns (MAMPs) of root-associated microbiota can trigger costly defenses at the expense of plant growth. However, beneficial rhizobacteria, such as Pseudomonas simiae WCS417, promote plant growth and induce systemic resistance without being warded off by local root immune responses. To investigate early r...
Significance
Plant roots nurture a large diversity of soil microbes via exudation of chemical compounds into the rhizosphere. In turn, beneficial root microbiota promote plant growth and immunity. The root-specific transcription factor MYB72 has emerged as a central regulator in this process. Here, we show that MYB72 regulates the excretion of the...
Plants live in close association with a myriad of microbes that are generally harmless. However, the minority of microbes that are pathogens can severely impact crop quality and yield, thereby endangering food security. By contrast, beneficial microbes provide plants with important services, such as enhanced nutrient uptake and protection against p...
Approximately 29% of all vascular plant species are unable to establish an arbuscular mycorrhizal (AM) symbiosis. Despite this, AM fungi (Rhizophagus spp.) are enriched in the root microbiome of the non‐host Arabidopsis thaliana and Arabidopsis roots become colonized when AM networks nurtured by host plants are available. Here, we investigated the...
Coumarins are a family of plant-derived secondary metabolites that are produced via the phenylpropanoid pathway. In the past decade, coumarins have emerged as iron-mobilizing compounds that are secreted by plant roots and aid in iron uptake from iron-deprived soils. Members of the coumarin family are found in many plant species. Besides their role...
The soil is vital for life on Earth and its biodiversity. However, being a non-renewable and threatened resource, preserving soil quality is crucial to maintain a range of ecosystem services critical to ecological balances, food production and human health. In an agricultural context, soil quality is often perceived as the ability to support field...
The soil, where plant roots grow, is a complex ecosystem with millions of microbial inhabitants residing in really high densities per gram of soil. These microbes, together with the functions they encode, are known as the microbiome. Plant microbiome members are as diverse as they are numerous. They can be detrimental, neutral or beneficial for the...
Growth-promoting bacteria can boost crop productivity in a sustainable way. Pseudomonas simiae WCS417 is a well-studied bacterium that promotes growth of many plant species. Upon colonization, WCS417 affects root system architecture resulting in an expanded root system. Both immunity and root system architecture, are controlled by root-cell-type sp...
The Green Revolution during the 50s and 60s was a milestone in the history of mankind. Based on the principles “higher yields, more food, less poverty and hunger,” it radically transformed agriculture and dramatically increased global food production (Khush, 2001). Despite the success, intensive agricultural practices that include the exhaustive us...
Bacterial flagellin is a potent host immune activator. Parys et al. (2021) and Colaianni et al. (2021) dissected effects of flagellin epitope variants on host immune detection and bacterial motility. They report in this issue of Cell Host & Microbe that Arabidopsis-associated bacterial microbiota differentially evolved flg22 variants that allow tun...
Background
Since the 1980s, numerous mutualistic Pseudomonas spp. strains have been used in studies on the biology of plant growth-promoting rhizobacteria (PGPR) and their interactions with host plants. In 1988, a strain from the Pseudomonas fluorescens group, WCS417, was isolated from lesions of wheat roots growing in a take-all disease-suppressiv...
Pseudomonas simiae WCS417 is a root-colonizing bacterium with well-established plant-beneficial effects. Upon colonization of Arabidopsis roots, WCS417 evades local root immune responses while triggering an induced systemic resistance (ISR) in the leaves. The early onset of ISR in roots shows similarities with the iron deficiency response, as both...
Plants shape their rhizosphere microbiome by secreting root exudates into the soil environment. Recently, root-exuded coumarins were identified as novel players in plant–microbiome communication. Beneficial members of the root-associated microbiome stimulate coumarin biosynthesis in roots and their excretion into the rhizosphere. The iron-mobilizin...
Looking forward includes looking back every now and then. In 2007 David Weller looked back at 30 years of biocontrol of soil-borne pathogens by Pseudomonas and signified that the progress made over decades of research provides a firm foundation to formulate current and future research questions. It has been recognized for more than a century that s...
Plants host a mesmerizing diversity of microbes inside and around their roots, known as the microbiome. The microbiome is composed mostly of fungi, bacteria, oomycetes and archaea that can be either pathogenic or beneficial for plant health and fitness. To grow healthy, plants need to surveil soil niches around the roots for the detection of pathog...
The root microbiome consists of commensal, pathogenic, and plant-beneficial microbes [1]. Most members of the root microbiome possess microbe-associated molecular patterns (MAMPs) similar to those of plant pathogens [2]. Their recog- nition can lead to the activation of host immunity and suppression of plant growth due to growth- defense tradeoffs...
Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial...
Plants roots host myriads of microbes, some of which enhance the defense potential of plants by activating a broad-spectrum immune response in leaves, known as induced systemic resistance (ISR). Nevertheless, establishment of this mutualistic interaction requires active suppression of local root immune responses to allow successful colonization. To...
Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial...
Figure S1. Alignment of flg22 peptides from flagellin of P. simiae WCS417 (flg22417) and P. aeruginosa PO1 (flg22Pa).
Figure S2. Venn diagrams of DEGs shared between Arabidopsis responses to flg22Pa or chitin in different studies.
Dataset S1. DEGs of Arabidopsis thaliana (AGI numbers of DEGs; FDR < 0.05; > twofold) in response to P. simiae WCS417, flg22417, flg22Pa or chitin treatment at four consecutive time points.
Dataset S3. Shared DEGs between WCS417, flg22417, flg22Pa and chitin datasets shown in Figure 2b (shared DEGs between WCS417, flg22417, flg22Pa and chitin treatments).
Table S1. List of primers used in this study.
Dataset S2. Lists of DEGs following flg22Pa and chitin treatment from this study (after filtering out genes not present in microarray probesets) and from the studies of Zipfel et al. (2004) (flg22Pa), Beck et al. (2014) (flg22Pa) and Wan et al. (2008) (chitooctaose) that were used for the comparisons presented in Figure S2.
Dataset S4. Upregulated and downregulated DEGs only in response to flg22417 selected from the comparison with WCS417 (Figure 5) and the GO processes they are involved in.
Dataset S5. DEGs in roots 4 h after IAA treatment, DEGs from this study after root exposure to WCS417, their overlapping genes and the processes they are involved in.
Suppressive composts represent a sustainable approach to combat soil-borne plant pathogens and an alternative to the ineffective chemical fungicides used against those. Nevertheless, suppressiveness to plant pathogens and reliability of composts is often inconsistent with unpredictable effects. While suppressiveness is usually attributed to the com...
Iron is an essential nutrient for most life on Earth because it functions as a crucial redox catalyst in many cellular processes. However, when present in excess iron can lead to the formation of harmful hydroxyl radicals. Hence, the cellular iron balance must be tightly controlled. Perturbation of iron homeostasis is a major strategy in host-patho...
Background
Plant growth-promoting rhizobacteria (PGPR) can protect plants against pathogenic microbes through a diversity of mechanisms including competition for nutrients, production of antibiotics, and stimulation of the host immune system, a phenomenon called induced systemic resistance (ISR). In the past 30 years, the Pseudomonas spp. PGPR stra...
Several bacterial and fungal strains have been evaluated as biocontrol agents (BCAs) against Verticillium dahliae. In these studies, the BCAs were applied as a root drenching inoculum; however, this application method may have an adverse effect on the native, beneficial for the plants, microbial community. In the present study, it was evaluated whe...
Verticillium wilt is a devastating disease of a wide range of herbaceous and woody plant hosts. It is incited by the soilborne fungus Verticillium dahliae. Management strategies are mainly focused on preventive measures. In a previous study, the efficacy of a non-pathogenic Fusarium oxysporum strain, designated as F2, isolated from a suppressive co...
Projects
Projects (2)
The aim of the project is to understand what are the early molecular events in plant roots following recognition of beneficial microbes. Ultimate goal is to elucidate how this transcriptional reprogramming can lead to growth promotion and induced systemic resistance.
Our aim is to understand how exudates released from roots in response to various environmental stresses can affect the structure and the activity of the microbiome. That will deepen our understanding in the mechanisms employed by microbes in order to establish an association with plant roots and compete for the same resources with other microbes in the rhizosphere. In a next step, selected strains can be tested for their ability to ameliorate abiotic stresses and even help plants combat various pathogens.
