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Ioannis Papasotiriou

Ioannis Papasotiriou
Research Genetic Cancer Centre International GmbH · Molecular Medicine

MD, PHD

About

136
Publications
23,554
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681
Citations
Citations since 2017
70 Research Items
474 Citations
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2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
Introduction
Colon carcinoma Biomarkers in Lung and Liver metastases prediction Drug development in NSCLC Biomarkers and durable targets identification in solid tumors
Additional affiliations
January 2012 - present
University of Thessaly

Publications

Publications (136)
Article
Full-text available
Antisense therapy is widely used as an alternative therapeutic option for various diseases. RNA interference might be effective in infections, through the degradation of messenger RNA and, therefore, translation process. Hence, proteins essential for microorganisms and viruses’ proliferation and metabolism are inhibited, leading to their eliminatio...
Article
Full-text available
Over the past few decades, drug discovery has greatly improved the outcomes for patients, but several challenges continue to hinder the rapid development of novel drugs. Addressing unmet clinical needs requires the pursuit of drug targets that have a higher likelihood to lead to the development of successful drugs. Here we describe a bioinformatic...
Article
At Research Genetic Cancer Centre, we have developed a novel method for the production of human monoclonal antibodies against a specific antigen of our choice (c-met) using isolated human blood cells. By mimicking nature, dendritic, CD4 and CD19 cells from healthy volunteers were driven towards Th2 immunity. Cell activation was succeeded by a cytok...
Article
Background This study presents preliminary results concerning the effectiveness of a novel immunotherapy in cancer. The proposed adoptive cellular therapy product contains a mixture of effector immune cells, specifically macrophages, NK cells, dendritic cells, cytotoxic T lymphocytes and monoclonal antibody producing plasma cells. Methods The resu...
Article
The objective of this study is the assessment of the cytotoxic effect of fenbendazole and its commercially available formulation, which is used for its antihelmintic properties. The formulation was tested for its efficacy as well as the determination of the ingredients with proliferation assays and analytical techniques. HPLC, LC-MS and NMR confirm...
Article
Full-text available
Background Patients with unresectable recurrent rectal cancer (RRC) or colorectal cancer (CRC) with liver metastases, refractory to at least two lines of traditional systemic therapy, may receive third line intraarterial chemotherapy (IC) and targeted therapy (TT) using drugs selected by chemosensitivity and tumor gene expression analyses of liquid...
Article
Patients with cancer require efficient treatment approaches as the mortality rate due to their disease is high. Conventional therapies, like chemotherapy and radiation, have severe side effects. Drug discovery is focusing on the development of alternative strategies that could have beneficial effects to the patients. Cellular therapies are potentia...
Article
Full-text available
After two years since the outbreak, the COVID-19 pandemic remains a global public health emergency. SARS-CoV-2 variants with substitutions on the spike (S) protein emerge increasing the risk of immune evasion and cross-species transmission. Here, we analyzed the evolution of the S protein as recorded in 276,712 samples collected before the start of...
Article
Full-text available
Background/aim: An early evaluation concerning the effectiveness of supportive oligonucleotide therapy (SOT) in cancer as a monotherapy and in combination with other types of treatment. Patients and methods: This study evaluated the clinical condition and performance status (Karnofsky-Index) of 95 patients, post-SOT administration. Furthermore,...
Article
A new method for analyzing brain complex dynamics and states is presented. This method constructs functional brain graphs and is comprised of two pylons: a) Operational Architectonics (OA) concept of brain and mind functioning. b) Network Neuroscience. In particular, the algorithm utilizes OA framework for a non-parametric segmentation of EEGs, whi...
Article
Full-text available
Gastric cancer is one of the most common and deadly cancers worldwide. Screening tests as well as tools for prediction of treatment outcomes and prognosis have been developed, but they have many limitations. The integration of liquid biopsy provided new aspects in screening and diagnosis of gastric cancer. In the present study, we used different te...
Article
Full-text available
Colorectal cancer is one of the most common types of cancer, and it can have a high mortality rate if left untreated or undiagnosed. The fact that CRC becomes symptomatic at advanced stages highlights the importance of early screening. The reference screening method for CRC is colonoscopy, an invasive, time-consuming procedure that requires sedatio...
Article
Full-text available
The objective of this study is the assessment of the cytotoxic effect of a commercially available fermented soy beverage Haelan 951, which is a supplement widely used by cancer patients. The main ingredient of the product is soy that is rich in flavonoids, especially isoflavone. The formulation was tested for its efficacy as well as the determinati...
Article
Full-text available
Natural compounds are used as pharmaceutical agents in modern drug development because they exhibit antioxidant, antimicrobial and anti-tumor properties. The extracts of Boswellia sacra have been used in the present study in order to determine the in vitro cytotoxic effect they might demonstrate against human cancer cell lines. The compounds of the...
Article
Full-text available
Despite the fact that COVID-19 vaccines are already available on the market, there have not been any effective FDA-approved drugs to treat this disease. There are several already known drugs that through drug repositioning have shown an inhibitory activity against SARS-CoV-2 RNA-dependent RNA polymerase. These drugs are included in the family of nu...
Article
Full-text available
The objective of this study is to improve and optimize the formulation of Genistein in capsules in order to result in a better pharmacokinetic profile comparing to existing commercial products. In order to do this, five different formulations of Genistein capsules were developed and examined by reviewing their disintegration and dissolution propert...
Article
Full-text available
Objectives: Circulating tumour cells (CTCs) from liquid biopsies provide an exceptional opportunity to obtain real-time tumour information and are under current investigation in several cancers, including cutaneous melanoma, but face significant drawbacks in terms of non-standardised methodology, low viable cell numbers and accuracy of CTC identif...
Article
Full-text available
The aim of this study is to evaluate the potential health effects of Tegaran Formula Zhen-Hua, a nutritional supplement used mainly by cancer patients. Its active ingredients and cytotoxicity was assessed with analytical methods and viability assays, respectively. The analytical methods consisted of dissolution, disintegration, HPLC, LC/MS, GC/MS a...
Article
Full-text available
Background: Although cisplatin is used for the treatment of more than half of cancer patients, its use is restricted by serious side effects as well as the development of cisplatin-resistant cancer cells, limiting its use. In RGCC we have synthesized an intermediate molecule in an ERK inhibitor synthesis process. Aims and objectives: The aim of...
Article
Full-text available
Emergence of coronaviruses poses a threat to global health and economy. The current outbreak of SARS-CoV-2 has infected more than 28,000,000 people and killed more than 915,000. To date, there is no treatment for coronavirus infections, making the development of therapies to prevent future epidemics of paramount importance. To this end, we collecte...
Article
Full-text available
Circulating tumour cells (CTCs) from liquid biopsies are under current investigation in several cancers, including epithelial ovarian cancer (EOC) but face significant drawbacks in terms of non-standardised methodology, low viable cell numbers and accuracy of CTC identification. In this pilot study, we report that chemosensitivity assays using liqu...
Article
Full-text available
Background Hepatic artery infusion (HAI) and drug selection by liquid biopsy precision oncotherapy are under investigation for the multidisciplinary treatment of unresectable colorectal liver metastases (CRCLM) in progression after systemic therapy. Here, we compare the safety and efficacy of third-line HAI followed by target therapy with drug regi...
Article
Full-text available
Although monoclonal antibodies are promising, a truly fully human antibody is yet to be produced. Current human antibodies have the human sequence, but are produced in either transgenic animals or in phages. The aim of this paper was to produce a truly human antibody directed against an epitope of our choice, secreted by human plasma cells. The tar...
Article
Full-text available
Objective: The aim of the present study is to analyze four herbal dietary supplements containing genistein by liquid chromatography, quantitative nuclear magnetic resonance (qNMR), and diffusion ordered spectroscopy (DOSY) 1H NMR. Methods: Quantification of the active ingredient, genistein, is carried out by high-performance liquid chromatography (...
Article
Full-text available
Background Third line innovative systemic treatments and loco-regional chemotherapy by hypoxic pelvic perfusion (HPP) have both been proposed for the treatment of unresectable not responsive recurrent rectal cancer (URRC). In the present study, we have compared the safety and efficacy of HPP/target therapy, using drug regimens selected by liquid bi...
Article
Full-text available
Despite the fact that there are several anticancer drugs available, cancer has evolved using different pathways inside the cell. The protein tyrosine phosphatases pathway is responsible for monitoring cell proliferation, diversity, migration, and metabolism. More specifically, the SHP2 protein, which is a member of the PTPs family, is closely relat...
Article
Full-text available
In this brief survey, various aspects of cancer complexity and how this complexity can be confronted using modern complex networks’ theory and gene expression datasets, are described. In particular, basic features of cancer as a complex system are noted, while the importance of gene expression data in cancer research and in reverse engineering of g...
Article
Full-text available
Background Cancer of unknown primary origin (CUP) represents a metastatic cancer with unidentified primary origin. An efficient cancer treatment algorithm is based on detection and characterization of the tumor’s origin. CUP is characterized by chromosomal instability; therefore the detection of chromosomal aberrations might contribute in tumor cha...
Article
Full-text available
e14656 Background: Lung cancer is the second most common type of cancer in both genders; however is the leading cause of cancer deaths. Adenocarcinoma constitutes more than 60% of non-small cell lung cancer (NSCLC), and accounts for approximately 40% of lung cancers. Therefore, early prediction, prognosis of the disease and treatment strategy is of...
Article
Full-text available
Analysis and comparison of gene expression profile among molecules, correlated with essential and crucial biological processes, is of primary importance in cancer research, since it provides significant info regarding the resistance to chemo/ radiotherapy, risk for relapse or prediction of metastasis etc. In this study, gene expression profile is u...
Article
Full-text available
The Ras–Raf–MEK1/2–ERK1/2 pathway is an attractive target for the development of anticancer agents because of the high prevalence of ERK activation in human cancers. However, resistance is often developed despite initial clinical response, most likely because of activation of cross-talk pathways. In Research Genetic Cancer Center (RGCC), we are in...
Article
Full-text available
Short gold nanorods were synthesized (average length 28.08 nm, average aspect ratio 3.54), which were functionalized with folic acid (FA) and 8-mercaptooctanoic acid (MOA) or 11-mercaptoundecanoic acid (MDA) and loaded with paclitaxel (PCT). FA was conjugated to the nanorods in order to render them targetable for cancer cells overexpressing folate...
Article
Full-text available
In Research Genetic Cancer Center (RGCC), we are in the process of synthesizing a novel ERK inhibitor. We have currently synthesized an intermediate molecule, RGCC169, that needed to be tested to confirm we are using the appropriate synthetic pathways. Because of the limited solubility the compound exhibits, a strategy had to be devised for the fre...
Poster
Introduction Genistein is a natural flavonoid in which the B ring is linked to the heterocyclic ring at the C3 and can occur in foods both in free and esterified form. Dietary supplements containing genistein have positive health effects in areas including prevention of breast, colon and prostate cancers, cardiovascular disease and post-menopausal...
Poster
Introduction: Breast cancer if one of the most common types of malignancy regarding females. In the recent years a new method for detecting malignancies has emerged. It involves detecting circulating tumor cells (CTCs) in the peripheral blood using flow cytometry. The present study makes use of a multiparameter flow cytometric panel and magnetic be...
Article
Full-text available
Background Minimizing the effects of transportation on the properties of biological material is a major challenge for the scientific community. The viability of cells is important in cases where their study is urgent for evaluation of treatment response or for the study of cancer progression. Circulating tumor cells (CTCs) constitute a cell subpopu...
Article
e13001 Background: A variety of chromosomal aberrations underlie developmental abnormalities and cancer. Use of techniques such as microarrays provides an opportunity to perform gene expression analysis. A recent approach is the use of array based comparative genomic hybridization (aCGH) to detect genomic abnormalities in human tumors. The aim of t...
Article
Full-text available
Title Bacterial detection in cancer samples. Background Bacterial contamination follow up is an integral part of quality control for blood sample handling and processing. Blood sample contamination is promoted by inappropriate blood drawing practices or laboratory environments. This study investigated the presence of Gram-positive bacteria in the s...
Article
Full-text available
Panagiotis Apostolou, Ioannis Papasotiriou Department of Molecular Medicine, Research Genetic Cancer Centre S.A. (R.G.C.C. S.A.), Florina, Greece Abstract: Checkpoint kinase 2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to th...
Article
Full-text available
Circulating tumor cells (CTCs) are a population of tumor-derived cells that detach from the primary tumor and initiate metastasis. However, the mechanisms of this process are still unknown. This phenomenon renders CTCs as a valuable resource for prognosis and diagnosis of cancer. The involvement of stemness transcription factors and markers, such a...
Article
Full-text available
Long interspersed nuclear element 1 (LINE-1 or L1) belongs to the non-long terminal repeat (non-LTR) retrotransposon family, which has been implicated in carcinogenesis and disease progression. Circulating tumor cells (CTCs) are also known to be involved in cancer progression. The present study aimed to compare the L1 expression between circulating...
Data
Ethical Approval of the present study. (JPG)
Data
Raw Data of PCR and FISH Experiments. (XLSX)
Poster
Full-text available
Relationshib between TMX-2 expression and stemness phenotype in breast carcinoma
Poster
Full-text available
L1 -retrotransosition and cancer stemm cell like phenotype
Article
Background/aim: We developed a novel platform to produce fully human antibodies that could be used as tools in cancer research. This study was based on maturation of B-cells into plasma cells and the subsequent secretion of fully human antibodies against a 9-mer peptide. Materials and methods: A peptide was introduced to dendritic cells, followe...
Poster
Full-text available
Gene expression pattern on CTCs in pancreatic carcinoma cases
Article
Full-text available
Circulating tumour cells (CTCs) are becoming very welcomed in the field of oncology, and they are also promising in the clinical field, in the area of drug development. Proof of concept that CTCs may correlate with the prognosis of cancer has been completed for many types already. Also, the CTCs may carry information that is relevant to the disease...
Article
Full-text available
A novel hybrid consisting of quinazoline and adenine moieties has been synthesized as a precursor of a potential biologically active target compound. The structure of 9-(4-methoxyquinazolin-2-yl)-9H-purin-6-amine (2) was characterized and confirmed using the following spectroscopic methods: LC-UV-MS, 1H-NMR, 13C-NMR and HSQC-NMR.
Poster
Full-text available
Differemces between primary breast carcinoma and brain metastases due to CSCs profile